Restriction map for pFN21AB6202
 (Circular) (Six-base) MAPSORT of: pFN21AB6202.seq  Check: 6278  from: 1  to: 6838



pFN21AB6202  6838 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:27  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4648     278 
   Size:         53      83     186     936    3112    2468

  Fragments arranged by size: 

                3112    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    4181    1937 
   Size:       2244    4594
 
Acc65I G'GTAC_C

Cuts at:   4159    4159 
   Size:       6838
 
AclI AA'CG_TT

Cuts at:   1563    4965    5338    1563 
   Size:       3402     373    3063

  Fragments arranged by size: 

                3402    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2095    2218    2295    3124    4901    6285    6553    1632 
   Size:        463     123      77     829    1777    1384     268    1917

  Fragments arranged by size: 

                1917    1777    1384     829     463     268     123      77
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6641
 
AflIII A'CryG_T

Cuts at:   1203    5737    6714    1203 
   Size:       4534     977    1327

  Fragments arranged by size: 

                4534    1327     977
 
AgeI A'CCGG_T

Cuts at:   5669    5669 
   Size:       6838
 
AhdI GACnn_n'nnGTC

Cuts at:   5567    5567 
   Size:       6838
 
AleI CACnn'nnGTG

Cuts at:   1166    3441    3750    1166 
   Size:       2275     309    4254

  Fragments arranged by size: 

                4254    2275     309
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6838
 
AlwNI CAG_nnn'CTG

Cuts at:   2044    2134    3525    4581    6153    2044 
   Size:         90    1391    1056    1572    2729

  Fragments arranged by size: 

                2729    1572    1391    1056      90
 
ApaLI G'TGCA_C

Cuts at:   3769    4895    6051    3769 
   Size:       1126    1156    4556

  Fragments arranged by size: 

                4556    1156    1126
 
ApoI r'AATT_y

Cuts at:   1493    2020    2460    2817    2901    3237    3489    3573    3657 
   Size:        527     440     357      84     336     252      84      84

Cuts at:   3657    3741    3805    3993    4147    4373    4525    6634    1493 
   Size:         84      64     188     154     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     527     440     357     336     252     226
                 188     154     152      84      84      84      84      64
            
 
AseI AT'TA_AT

Cuts at:    160    1032    5392     160 
   Size:        872    4360    1606

  Fragments arranged by size: 

                4360    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6838
 
AvaI C'yCGr_G

Cuts at:   1870    1943    4163    1870 
   Size:         73    2220    4545

  Fragments arranged by size: 

                4545    2220      73
 
BamHI G'GATC_C

Cuts at:   4168    4535    4168 
   Size:        367    6471
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    4159    5614     618 
   Size:        325     217     627    2372    1455    1842

  Fragments arranged by size: 

                2372    1842    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2181    4157    1391 
   Size:        790    1976    4072

  Fragments arranged by size: 

                4072    1976     790
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    5867
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    5029    5063    1903 
   Size:         34      50      34    3008      34    3678

  Fragments arranged by size: 

                3678    3008      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4730    5940    4730 
   Size:       1210    5628
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6838
 
BfrBI ATG'CAT

Cuts at:   3263    3649    3263 
   Size:        386    6452
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5449     136 
   Size:        107     122      71     631     605    3777    1525

  Fragments arranged by size: 

                3777    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6833    6833 
   Size:       6838
 
BlpI GC'TnA_GC

Cuts at:   4255    4255 
   Size:       6838
 
Bme1580I G_kGCm'C

Cuts at:   1790    3773    4899    6055    1790 
   Size:       1983    1126    1156    2573

  Fragments arranged by size: 

                2573    1983    1156    1126
 
BmgBI CAC'GTC

Cuts at:   1318    2098    1318 
   Size:        780    6058
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4569    5527     442 
   Size:        416      53      83     399      24    3152     958    1753

  Fragments arranged by size: 

                3152    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6838
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3188    3188 
   Size:       6838
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2195    3603    5498    1239 
   Size:        956    1408    1895    2579

  Fragments arranged by size: 

                2579    1895    1408     956
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2789    3962    4963    5828    6126    2789 
   Size:       1173    1001     865     298    3501

  Fragments arranged by size: 

                3501    1173    1001     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5501     882 
   Size:        617    4002    2219

  Fragments arranged by size: 

                4002    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4629     493 
   Size:        711    3425    2702

  Fragments arranged by size: 

                3425    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   3480    6832    3480 
   Size:       3352    3486
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4645    5027    5662     275 
   Size:         53      83     186     936    3112     382     635    1451

  Fragments arranged by size: 

                3112    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3665    5271    5669    5943    6090    6501    3665 
   Size:       1606     398     274     147     411    4002

  Fragments arranged by size: 

                4002    1606     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6613

  Fragments arranged by size: 

                6613     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2135    2282    1186 
   Size:        949     147    5742

  Fragments arranged by size: 

                5742     949     147
 
BseYI C'CCAG_C

Cuts at:   6041    6537    6041 
   Size:        496    6342
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    6265
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4288    5049    6077    1540 
   Size:        275     186    2287     761    1028    2301

  Fragments arranged by size: 

                2301    2287    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2181    3773    4899    4984    6055    2181 
   Size:       1592    1126      85    1071    2964

  Fragments arranged by size: 

                2964    1592    1126    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2244    3185    3362    3521    3614    3941    4349    4442    2244 
   Size:        941     177     159      93     327     408      93    4640

  Fragments arranged by size: 

                4640     941     408     327     177     159      93      93
            
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2181    3773    4157    4899    4984    6055    1391 
   Size:        399     391    1592     384     742      85    1071    2174

  Fragments arranged by size: 

                2174    1592    1071     742     399     391     384      85
            
 
BspEI T'CCGG_A

Cuts at:   6501    6501 
   Size:       6838
 
BspHI T'CATG_A

Cuts at:   4727    6457    4727 
   Size:       1730    5108
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3789    4193     844 
   Size:       2945     404    3489

  Fragments arranged by size: 

                3489    2945     404
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4725    1394 
   Size:        429    2902    3507

  Fragments arranged by size: 

                3507    2902     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2662    5333    5507      65 
   Size:       1183    1414    2671     174    1396

  Fragments arranged by size: 

                2671    1414    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5482    5669    1700 
   Size:        110    3672     187    2869

  Fragments arranged by size: 

                3672    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6838
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6838
 
BssSI C'ACGA_G

Cuts at:   2554    4898    5910    2554 
   Size:       2344    1012    3482

  Fragments arranged by size: 

                3482    2344    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3609     794 
   Size:       2815    4023
 
BstBI TT'CG_AA

Cuts at:   5642    5642 
   Size:       6838
 
BstEII G'GTnAC_C

Cuts at:   1184    2765    1184 
   Size:       1581    5257
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2930    3107    3443    2930 
   Size:        177     336    6325

  Fragments arranged by size: 

                6325     336     177
 
BstYI r'GATC_y

Cuts at:   1972    2066    4168    4535    4920    4937    6389    6475    6487 
   Size:         94    2102     367     385      17    1452      86      12

Cuts at:   6487    6833    1972 
   Size:        346    1977

  Fragments arranged by size: 

                2102    1977    1452     385     367     346      94      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2037    2037 
   Size:       6838
 
BtgI C'CryG_G

Cuts at:    513    1065    2103     513 
   Size:        552    1038    5248

  Fragments arranged by size: 

                5248    1038     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    6099

  Fragments arranged by size: 

                6099     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2205    3190    3274    3526    3610    3694    4354    5148    5168 
   Size:        985      84     252      84      84     660     794      20

Cuts at:   5168    2205 
   Size:       3875

  Fragments arranged by size: 

                3875     985     794     660     252      84      84      84
                  20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6838
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    3004    3039     529 
   Size:         35    2440      35    4328

  Fragments arranged by size: 

                4328    2440      35      35
 
DraI TTT'AAA

Cuts at:   4142    4142 
   Size:       6838
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5845     809 
   Size:        507    4529    1802

  Fragments arranged by size: 

                4529    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2788    4285    5174 
   Size:         54    1425      50     275      18     958    1497     889

Cuts at:   5174    6803       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1497    1425     958     889     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4285    1537 
   Size:        275    2473    4090

  Fragments arranged by size: 

                4090    2473     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2198    2719    3143    4768    1353 
   Size:        845     521     424    1625    3423

  Fragments arranged by size: 

                3423    1625     845     521     424
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5425    5799    5945     387 
   Size:       1064     138    3836     374     146    1280

  Fragments arranged by size: 

                3836    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2179    2179 
   Size:       6838
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2095    2195    2218    2295    3124    3603    4901 
   Size:        393     463     100      23      77     829     479    1298

Cuts at:   4901    5498    6285    6553    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524    1298     829     787     597     479     463     393
                 268     100      77      23
 
EcoO109I rG'GnC_Cy

Cuts at:   2041    2533    2559    2041 
   Size:        492      26    6320

  Fragments arranged by size: 

                6320     492      26
 
EcoRI G'AATT_C

Cuts at:   2901    3489    3657    3741    3993    4147    4525    2901 
   Size:        588     168      84     252     154     378    5214

  Fragments arranged by size: 

                5214     588     378     252     168     154      84
 
FspI TGC'GCA

Cuts at:   5344    5344 
   Size:       6838
 
HaeII r_GCGC'y

Cuts at:   1678    5985    1678 
   Size:       4307    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3118    3150    3382    3414    3886 
   Size:         32     135      32    1546      32     232      32     472

Cuts at:   3886    3918    5479    5511    5553    5585    1373 
   Size:         32    1561      32      42      32    2626

  Fragments arranged by size: 

                2626    1561    1546     472     232     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3300    4182    4428     669 
   Size:        988     281    1362     882     246    3079

  Fragments arranged by size: 

                3079    1362     988     882     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2346     748 
   Size:       1598    5240
 
HpaI GTT'AAC

Cuts at:   4428    4428 
   Size:       6838
 
KpnI G_GTAC'C

Cuts at:   4163    4163 
   Size:       6838
 
MfeI C'AATT_G

Cuts at:   2518    4437    2518 
   Size:       1919    4919
 
MluI A'CGCG_T

Cuts at:   6714    6714 
   Size:       6838
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5952    6136    1305 
   Size:       4647     184    2007

  Fragments arranged by size: 

                4647    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2790      10 
   Size:         54    1425     343     958    4058

  Fragments arranged by size: 

                4058    1425     958     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2928    2994    3105    3246    3441    3750    4796 
   Size:        648    1762      66     111     141     195     309    1046

Cuts at:   4796    5155    5314     518 
   Size:        359     159    2042

  Fragments arranged by size: 

                2042    1762    1046     648     359     309     195     159
                 141     111      66
 
MspA1I CmG'CkG

Cuts at:   1606    4254    4931    6079    6324    1606 
   Size:       2648     677    1148     245    2120

  Fragments arranged by size: 

                2648    2120    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    6286
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6838
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6838
 
NotI GC'GGCC_GC

Cuts at:   4285    4285 
   Size:       6838
 
NsiI A_TGCA'T

Cuts at:   3265    3651    3265 
   Size:        386    6452
 
NspI r_CATG'y

Cuts at:   1132    1229    3267    3649    4196    5741    1132 
   Size:         97    2038     382     547    1545    2229

  Fragments arranged by size: 

                2229    2038    1545     547     382      97
 
PasI CC'CwG_GG

Cuts at:   1348    2537    1348 
   Size:       1189    5649
 
PciI A'CATG_T

Cuts at:   5737    5737 
   Size:       6838
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6838
 
PmeI GTTT'AAAC

Cuts at:   4142    4142 
   Size:       6838
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4907    4939    6420    6452    4907 
   Size:         32    1481      32    5293

  Fragments arranged by size: 

                5293    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2041    2533    2041 
   Size:        492    6346
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6838
 
PsiI TTA'TAA

Cuts at:   4408    4408 
   Size:       6838
 
PstI C_TGCA'G

Cuts at:    830    2729    4190     830 
   Size:       1899    1461    3478

  Fragments arranged by size: 

                3478    1899    1461
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6838
 
SacI G_AGCT'C

Cuts at:   2181    2181 
   Size:       6838
 
SalI G'TCGA_C

Cuts at:   1936    4180    1936 
   Size:       2244    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2719    2719 
   Size:       6838
 
SbfI CC_TGCA'GG

Cuts at:   4190    4190 
   Size:       6838
 
ScaI AGT'ACT

Cuts at:   5086    6798    5086 
   Size:       1712    5126
 
SfcI C'TryA_G

Cuts at:    826    1045    2182    2725    4186    5321    6002    6193     826 
   Size:        219    1137     543    1461    1135     681     191    1471

  Fragments arranged by size: 

                1471    1461    1137    1135     681     543     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6838
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6838
 
SmaI CCC'GGG

Cuts at:   1872    4165    1872 
   Size:       2293    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2804    3977    4942    5843    6105     820 
   Size:        197     926     861    1173     965     901     262    1553

  Fragments arranged by size: 

                1553    1173     965     926     901     861     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    4629     493 
   Size:       4136    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6838
 
SphI G_CATG'C

Cuts at:   1132    3267    3649    4196    1132 
   Size:       2135     382     547    3774

  Fragments arranged by size: 

                3774    2135     547     382
 
SspI AAT'ATT

Cuts at:      5      52    2737    4762       5 
   Size:         47    2685    2025    2081

  Fragments arranged by size: 

                2685    2081    2025      47
 
StuI AGG'CCT

Cuts at:   1517    3368    3788    3844    4040    1517 
   Size:       1851     420      56     196    4315

  Fragments arranged by size: 

                4315    1851     420     196      56
 
StyI C'CwwG_G

Cuts at:    513    1065    4277     513 
   Size:        552    3212    3074

  Fragments arranged by size: 

                3212    3074     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2885    3557    4865    4882    5035    5220    5665 
   Size:       1255    1305     672    1308      17     153     185     445

Cuts at:   5665     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1308    1305    1255     672     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    5084    6796      96 
   Size:        274      80      33      51    1443    3107    1712     138

  Fragments arranged by size: 

                3107    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6798    6830    6798 
   Size:         32    6806
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6838
 
XbaI T'CTAG_A

Cuts at:   4174    4174 
   Size:       6838
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6838
 
XmaI C'CCGG_G

Cuts at:   1870    4163    1870 
   Size:       2293    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3452    3620    3704    3788    3956    4967    3452 
   Size:        168      84      84     168    1011    5323

  Fragments arranged by size: 

                5323    1011     168     168      84      84
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4646     276 
   Size:         53      83     186     936    3112    2468

  Fragments arranged by size: 

                3112    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PstI     PvuI     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI   Bpu10I    BsiWI    BsmBI  BstZ17I   DraIII    EcoNI    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     PacI     PfoI     PmlI   PspOMI    PspXI     PsrI    PvuII    RsrII
    SacII    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com