(Circular) (Six-base) MAPSORT of: pFN21AB6202.seq Check: 6278 from: 1 to: 6838 pFN21AB6202 6838 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4648 278 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4181 1937 Size: 2244 4594 Acc65I G'GTAC_C Cuts at: 4159 4159 Size: 6838 AclI AA'CG_TT Cuts at: 1563 4965 5338 1563 Size: 3402 373 3063 Fragments arranged by size: 3402 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2095 2218 2295 3124 4901 6285 6553 1632 Size: 463 123 77 829 1777 1384 268 1917 Fragments arranged by size: 1917 1777 1384 829 463 268 123 77 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6641 AflIII A'CryG_T Cuts at: 1203 5737 6714 1203 Size: 4534 977 1327 Fragments arranged by size: 4534 1327 977 AgeI A'CCGG_T Cuts at: 5669 5669 Size: 6838 AhdI GACnn_n'nnGTC Cuts at: 5567 5567 Size: 6838 AleI CACnn'nnGTG Cuts at: 1166 3441 3750 1166 Size: 2275 309 4254 Fragments arranged by size: 4254 2275 309 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6838 AlwNI CAG_nnn'CTG Cuts at: 2044 2134 3525 4581 6153 2044 Size: 90 1391 1056 1572 2729 Fragments arranged by size: 2729 1572 1391 1056 90 ApaLI G'TGCA_C Cuts at: 3769 4895 6051 3769 Size: 1126 1156 4556 Fragments arranged by size: 4556 1156 1126 ApoI r'AATT_y Cuts at: 1493 2020 2460 2817 2901 3237 3489 3573 3657 Size: 527 440 357 84 336 252 84 84 Cuts at: 3657 3741 3805 3993 4147 4373 4525 6634 1493 Size: 84 64 188 154 226 152 2109 1697 Fragments arranged by size: 2109 1697 527 440 357 336 252 226 188 154 152 84 84 84 84 64 AseI AT'TA_AT Cuts at: 160 1032 5392 160 Size: 872 4360 1606 Fragments arranged by size: 4360 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 AvaI C'yCGr_G Cuts at: 1870 1943 4163 1870 Size: 73 2220 4545 Fragments arranged by size: 4545 2220 73 BamHI G'GATC_C Cuts at: 4168 4535 4168 Size: 367 6471 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4159 5614 618 Size: 325 217 627 2372 1455 1842 Fragments arranged by size: 2372 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2181 4157 1391 Size: 790 1976 4072 Fragments arranged by size: 4072 1976 790 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5867 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5029 5063 1903 Size: 34 50 34 3008 34 3678 Fragments arranged by size: 3678 3008 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4730 5940 4730 Size: 1210 5628 BclI T'GATC_A Cuts at: 1548 1548 Size: 6838 BfrBI ATG'CAT Cuts at: 3263 3649 3263 Size: 386 6452 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5449 136 Size: 107 122 71 631 605 3777 1525 Fragments arranged by size: 3777 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6833 6833 Size: 6838 BlpI GC'TnA_GC Cuts at: 4255 4255 Size: 6838 Bme1580I G_kGCm'C Cuts at: 1790 3773 4899 6055 1790 Size: 1983 1126 1156 2573 Fragments arranged by size: 2573 1983 1156 1126 BmgBI CAC'GTC Cuts at: 1318 2098 1318 Size: 780 6058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4569 5527 442 Size: 416 53 83 399 24 3152 958 1753 Fragments arranged by size: 3152 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6838 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3188 3188 Size: 6838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 3603 5498 1239 Size: 956 1408 1895 2579 Fragments arranged by size: 2579 1895 1408 956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2789 3962 4963 5828 6126 2789 Size: 1173 1001 865 298 3501 Fragments arranged by size: 3501 1173 1001 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5501 882 Size: 617 4002 2219 Fragments arranged by size: 4002 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4629 493 Size: 711 3425 2702 Fragments arranged by size: 3425 2702 711 BsaBI GATnn'nnATC Cuts at: 3480 6832 3480 Size: 3352 3486 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4645 5027 5662 275 Size: 53 83 186 936 3112 382 635 1451 Fragments arranged by size: 3112 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3665 5271 5669 5943 6090 6501 3665 Size: 1606 398 274 147 411 4002 Fragments arranged by size: 4002 1606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6613 Fragments arranged by size: 6613 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2282 1186 Size: 949 147 5742 Fragments arranged by size: 5742 949 147 BseYI C'CCAG_C Cuts at: 6041 6537 6041 Size: 496 6342 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6265 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4288 5049 6077 1540 Size: 275 186 2287 761 1028 2301 Fragments arranged by size: 2301 2287 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 3773 4899 4984 6055 2181 Size: 1592 1126 85 1071 2964 Fragments arranged by size: 2964 1592 1126 1071 85 BsmI GAATG_Cn' Cuts at: 2244 3185 3362 3521 3614 3941 4349 4442 2244 Size: 941 177 159 93 327 408 93 4640 Fragments arranged by size: 4640 941 408 327 177 159 93 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 3773 4157 4899 4984 6055 1391 Size: 399 391 1592 384 742 85 1071 2174 Fragments arranged by size: 2174 1592 1071 742 399 391 384 85 BspEI T'CCGG_A Cuts at: 6501 6501 Size: 6838 BspHI T'CATG_A Cuts at: 4727 6457 4727 Size: 1730 5108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3789 4193 844 Size: 2945 404 3489 Fragments arranged by size: 3489 2945 404 BsrBI CCG'CTC Cuts at: 1394 1823 4725 1394 Size: 429 2902 3507 Fragments arranged by size: 3507 2902 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2662 5333 5507 65 Size: 1183 1414 2671 174 1396 Fragments arranged by size: 2671 1414 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5482 5669 1700 Size: 110 3672 187 2869 Fragments arranged by size: 3672 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6838 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6838 BssSI C'ACGA_G Cuts at: 2554 4898 5910 2554 Size: 2344 1012 3482 Fragments arranged by size: 3482 2344 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3609 794 Size: 2815 4023 BstBI TT'CG_AA Cuts at: 5642 5642 Size: 6838 BstEII G'GTnAC_C Cuts at: 1184 2765 1184 Size: 1581 5257 BstXI CCAn_nnnn'nTGG Cuts at: 2930 3107 3443 2930 Size: 177 336 6325 Fragments arranged by size: 6325 336 177 BstYI r'GATC_y Cuts at: 1972 2066 4168 4535 4920 4937 6389 6475 6487 Size: 94 2102 367 385 17 1452 86 12 Cuts at: 6487 6833 1972 Size: 346 1977 Fragments arranged by size: 2102 1977 1452 385 367 346 94 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2037 2037 Size: 6838 BtgI C'CryG_G Cuts at: 513 1065 2103 513 Size: 552 1038 5248 Fragments arranged by size: 5248 1038 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6099 Fragments arranged by size: 6099 683 56 BtsI GCAGTG_nn' Cuts at: 2205 3190 3274 3526 3610 3694 4354 5148 5168 Size: 985 84 252 84 84 660 794 20 Cuts at: 5168 2205 Size: 3875 Fragments arranged by size: 3875 985 794 660 252 84 84 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6838 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3004 3039 529 Size: 35 2440 35 4328 Fragments arranged by size: 4328 2440 35 35 DraI TTT'AAA Cuts at: 4142 4142 Size: 6838 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5845 809 Size: 507 4529 1802 Fragments arranged by size: 4529 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2788 4285 5174 Size: 54 1425 50 275 18 958 1497 889 Cuts at: 5174 6803 8 Size: 1629 43 Fragments arranged by size: 1629 1497 1425 958 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4285 1537 Size: 275 2473 4090 Fragments arranged by size: 4090 2473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2198 2719 3143 4768 1353 Size: 845 521 424 1625 3423 Fragments arranged by size: 3423 1625 845 521 424 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5425 5799 5945 387 Size: 1064 138 3836 374 146 1280 Fragments arranged by size: 3836 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2179 2179 Size: 6838 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2195 2218 2295 3124 3603 4901 Size: 393 463 100 23 77 829 479 1298 Cuts at: 4901 5498 6285 6553 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1298 829 787 597 479 463 393 268 100 77 23 EcoO109I rG'GnC_Cy Cuts at: 2041 2533 2559 2041 Size: 492 26 6320 Fragments arranged by size: 6320 492 26 EcoRI G'AATT_C Cuts at: 2901 3489 3657 3741 3993 4147 4525 2901 Size: 588 168 84 252 154 378 5214 Fragments arranged by size: 5214 588 378 252 168 154 84 FspI TGC'GCA Cuts at: 5344 5344 Size: 6838 HaeII r_GCGC'y Cuts at: 1678 5985 1678 Size: 4307 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3118 3150 3382 3414 3886 Size: 32 135 32 1546 32 232 32 472 Cuts at: 3886 3918 5479 5511 5553 5585 1373 Size: 32 1561 32 42 32 2626 Fragments arranged by size: 2626 1561 1546 472 232 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 4182 4428 669 Size: 988 281 1362 882 246 3079 Fragments arranged by size: 3079 1362 988 882 281 246 HindIII A'AGCT_T Cuts at: 748 2346 748 Size: 1598 5240 HpaI GTT'AAC Cuts at: 4428 4428 Size: 6838 KpnI G_GTAC'C Cuts at: 4163 4163 Size: 6838 MfeI C'AATT_G Cuts at: 2518 4437 2518 Size: 1919 4919 MluI A'CGCG_T Cuts at: 6714 6714 Size: 6838 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5952 6136 1305 Size: 4647 184 2007 Fragments arranged by size: 4647 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2790 10 Size: 54 1425 343 958 4058 Fragments arranged by size: 4058 1425 958 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2928 2994 3105 3246 3441 3750 4796 Size: 648 1762 66 111 141 195 309 1046 Cuts at: 4796 5155 5314 518 Size: 359 159 2042 Fragments arranged by size: 2042 1762 1046 648 359 309 195 159 141 111 66 MspA1I CmG'CkG Cuts at: 1606 4254 4931 6079 6324 1606 Size: 2648 677 1148 245 2120 Fragments arranged by size: 2648 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6286 NdeI CA'TA_TG Cuts at: 387 387 Size: 6838 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6838 NotI GC'GGCC_GC Cuts at: 4285 4285 Size: 6838 NsiI A_TGCA'T Cuts at: 3265 3651 3265 Size: 386 6452 NspI r_CATG'y Cuts at: 1132 1229 3267 3649 4196 5741 1132 Size: 97 2038 382 547 1545 2229 Fragments arranged by size: 2229 2038 1545 547 382 97 PasI CC'CwG_GG Cuts at: 1348 2537 1348 Size: 1189 5649 PciI A'CATG_T Cuts at: 5737 5737 Size: 6838 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6838 PmeI GTTT'AAAC Cuts at: 4142 4142 Size: 6838 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4907 4939 6420 6452 4907 Size: 32 1481 32 5293 Fragments arranged by size: 5293 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2041 2533 2041 Size: 492 6346 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6838 PsiI TTA'TAA Cuts at: 4408 4408 Size: 6838 PstI C_TGCA'G Cuts at: 830 2729 4190 830 Size: 1899 1461 3478 Fragments arranged by size: 3478 1899 1461 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6838 SacI G_AGCT'C Cuts at: 2181 2181 Size: 6838 SalI G'TCGA_C Cuts at: 1936 4180 1936 Size: 2244 4594 SapI GCTCTTCn'nnn_ Cuts at: 2719 2719 Size: 6838 SbfI CC_TGCA'GG Cuts at: 4190 4190 Size: 6838 ScaI AGT'ACT Cuts at: 5086 6798 5086 Size: 1712 5126 SfcI C'TryA_G Cuts at: 826 1045 2182 2725 4186 5321 6002 6193 826 Size: 219 1137 543 1461 1135 681 191 1471 Fragments arranged by size: 1471 1461 1137 1135 681 543 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6838 SmaI CCC'GGG Cuts at: 1872 4165 1872 Size: 2293 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2804 3977 4942 5843 6105 820 Size: 197 926 861 1173 965 901 262 1553 Fragments arranged by size: 1553 1173 965 926 901 861 262 197 SnaBI TAC'GTA Cuts at: 493 4629 493 Size: 4136 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6838 SphI G_CATG'C Cuts at: 1132 3267 3649 4196 1132 Size: 2135 382 547 3774 Fragments arranged by size: 3774 2135 547 382 SspI AAT'ATT Cuts at: 5 52 2737 4762 5 Size: 47 2685 2025 2081 Fragments arranged by size: 2685 2081 2025 47 StuI AGG'CCT Cuts at: 1517 3368 3788 3844 4040 1517 Size: 1851 420 56 196 4315 Fragments arranged by size: 4315 1851 420 196 56 StyI C'CwwG_G Cuts at: 513 1065 4277 513 Size: 552 3212 3074 Fragments arranged by size: 3212 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2885 3557 4865 4882 5035 5220 5665 Size: 1255 1305 672 1308 17 153 185 445 Cuts at: 5665 325 Size: 1498 Fragments arranged by size: 1498 1308 1305 1255 672 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5084 6796 96 Size: 274 80 33 51 1443 3107 1712 138 Fragments arranged by size: 3107 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6798 6830 6798 Size: 32 6806 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6838 XbaI T'CTAG_A Cuts at: 4174 4174 Size: 6838 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6838 XmaI C'CCGG_G Cuts at: 1870 4163 1870 Size: 2293 4545 XmnI GAAnn'nnTTC Cuts at: 3452 3620 3704 3788 3956 4967 3452 Size: 168 84 84 168 1011 5323 Fragments arranged by size: 5323 1011 168 168 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4646 276 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI