(Circular) (Six-base) MAPSORT of: pFN21AB6194.seq Check: 1481 from: 1 to: 6823 pFN21AB6194 6823 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4633 278 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2712 3507 4166 1937 Size: 775 795 659 4594 Fragments arranged by size: 4594 795 775 659 Acc65I G'GTAC_C Cuts at: 4144 4144 Size: 6823 AclI AA'CG_TT Cuts at: 1563 4950 5323 1563 Size: 3387 373 3063 Fragments arranged by size: 3387 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2892 2920 3539 4886 6270 6538 1632 Size: 1260 28 619 1347 1384 268 1917 Fragments arranged by size: 1917 1384 1347 1260 619 268 28 AfeI AGC'GCT Cuts at: 2877 2877 Size: 6823 AflII C'TTAA_G Cuts at: 820 1017 2914 820 Size: 197 1897 4729 Fragments arranged by size: 4729 1897 197 AflIII A'CryG_T Cuts at: 1203 3333 3591 5722 6699 1203 Size: 2130 258 2131 977 1327 Fragments arranged by size: 2131 2130 1327 977 258 AgeI A'CCGG_T Cuts at: 5654 5654 Size: 6823 AhdI GACnn_n'nnGTC Cuts at: 2047 3691 5552 2047 Size: 1644 1861 3318 Fragments arranged by size: 3318 1861 1644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6823 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2825 1184 Size: 1641 5182 AlwNI CAG_nnn'CTG Cuts at: 3089 4566 6138 3089 Size: 1477 1572 3774 Fragments arranged by size: 3774 1572 1477 ApaLI G'TGCA_C Cuts at: 2629 3274 4880 6036 2629 Size: 645 1606 1156 3416 Fragments arranged by size: 3416 1606 1156 645 ApoI r'AATT_y Cuts at: 1493 2402 3197 4004 4132 4358 4510 6619 1493 Size: 909 795 807 128 226 152 2109 1697 Fragments arranged by size: 2109 1697 909 807 795 226 152 128 AseI AT'TA_AT Cuts at: 160 1032 5377 160 Size: 872 4345 1606 Fragments arranged by size: 4345 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 AvaI C'yCGr_G Cuts at: 1870 1943 4100 4148 1870 Size: 73 2157 48 4545 Fragments arranged by size: 4545 2157 73 48 BamHI G'GATC_C Cuts at: 4153 4520 4153 Size: 367 6456 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2444 4144 5599 618 Size: 325 217 627 657 1700 1455 1842 Fragments arranged by size: 1842 1700 1455 657 627 325 217 BanII G_rGCy'C Cuts at: 1391 2271 2729 3765 4142 1391 Size: 880 458 1036 377 4072 Fragments arranged by size: 4072 1036 880 458 377 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2315 3178 928 Size: 971 416 863 4573 Fragments arranged by size: 4573 971 863 416 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2474 2508 3976 4010 5014 5048 1903 Size: 34 537 34 1468 34 1004 34 3678 Fragments arranged by size: 3678 1468 1004 537 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4715 5925 4715 Size: 1210 5613 BclI T'GATC_A Cuts at: 1548 4050 1548 Size: 2502 4321 BfrBI ATG'CAT Cuts at: 2795 2795 Size: 6823 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5434 136 Size: 107 122 71 631 605 3762 1525 Fragments arranged by size: 3762 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2931 3651 6818 2931 Size: 720 3167 2936 Fragments arranged by size: 3167 2936 720 BlpI GC'TnA_GC Cuts at: 4240 4240 Size: 6823 Bme1580I G_kGCm'C Cuts at: 1790 2633 3278 4884 6040 1790 Size: 843 645 1606 1156 2573 Fragments arranged by size: 2573 1606 1156 843 645 BmgBI CAC'GTC Cuts at: 1318 2473 1318 Size: 1155 5668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3928 4554 5512 Size: 416 53 83 399 24 2511 626 958 Cuts at: 5512 442 Size: 1753 Fragments arranged by size: 2511 1753 958 626 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6823 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2290 2290 Size: 6823 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3830 5483 1239 Size: 2591 1653 2579 Fragments arranged by size: 2591 2579 1653 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2322 2768 4948 5813 6111 2322 Size: 446 2180 865 298 3034 Fragments arranged by size: 3034 2180 865 446 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2966 5486 882 Size: 617 1467 2520 2219 Fragments arranged by size: 2520 2219 1467 617 BsaAI yAC'GTr Cuts at: 493 1204 4614 493 Size: 711 3410 2702 Fragments arranged by size: 3410 2702 711 BsaBI GATnn'nnATC Cuts at: 2138 6817 2138 Size: 4679 2144 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4630 5012 5647 275 Size: 53 83 186 936 3097 382 635 1451 Fragments arranged by size: 3097 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2947 5256 5654 5928 6075 6486 2947 Size: 2309 398 274 147 411 3284 Fragments arranged by size: 3284 2309 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3035 3065 3305 3335 3578 Size: 30 165 30 1632 30 240 30 243 Cuts at: 3578 3608 1178 Size: 30 4393 Fragments arranged by size: 4393 1632 243 240 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3770 3773 1186 Size: 2584 3 4236 Fragments arranged by size: 4236 2584 3 BseYI C'CCAG_C Cuts at: 3658 3868 6026 6522 3658 Size: 210 2158 496 3959 Fragments arranged by size: 3959 2158 496 210 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2311 1163 Size: 573 575 5675 Fragments arranged by size: 5675 575 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3962 4273 5034 6062 1540 Size: 275 186 1961 311 761 1028 2301 Fragments arranged by size: 2301 1961 1028 761 311 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 2271 2633 2897 3278 3765 4048 4884 4969 Size: 171 362 264 381 487 283 836 85 Cuts at: 4969 6040 2100 Size: 1071 2883 Fragments arranged by size: 2883 1071 836 487 381 362 283 264 171 85 BsmI GAATG_Cn' Cuts at: 2352 2362 4334 4427 2352 Size: 10 1972 93 4748 Fragments arranged by size: 4748 1972 93 10 BsmBI CGTCTCn'nnnn_ Cuts at: 2402 2402 Size: 6823 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2271 2633 2729 2897 3278 3765 Size: 399 310 171 362 96 168 381 487 Cuts at: 3765 4048 4142 4884 4969 6040 1391 Size: 283 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 487 399 381 362 310 283 171 168 96 94 85 BspEI T'CCGG_A Cuts at: 6486 6486 Size: 6823 BspHI T'CATG_A Cuts at: 4092 4712 6442 4092 Size: 620 1730 4473 Fragments arranged by size: 4473 1730 620 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4178 844 Size: 3334 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4111 4710 1394 Size: 429 2288 599 3507 Fragments arranged by size: 3507 2288 599 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2590 5318 5492 65 Size: 1183 1342 2728 174 1396 Fragments arranged by size: 2728 1396 1342 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5467 5654 1700 Size: 110 3657 187 2869 Fragments arranged by size: 3657 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3341 96 Size: 3245 3578 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6823 BssSI C'ACGA_G Cuts at: 2483 2534 4883 5895 2483 Size: 51 2349 1012 3411 Fragments arranged by size: 3411 2349 1012 51 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6823 BstBI TT'CG_AA Cuts at: 5627 5627 Size: 6823 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6823 BstXI CCAn_nnnn'nTGG Cuts at: 3781 3781 Size: 6823 BstYI r'GATC_y Cuts at: 1972 2931 3651 4153 4520 4905 4922 6374 6460 Size: 959 720 502 367 385 17 1452 86 Cuts at: 6460 6472 6818 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 959 720 502 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3027 3027 Size: 6823 BtgI C'CryG_G Cuts at: 513 1065 2262 3742 3800 513 Size: 552 1197 1480 58 3536 Fragments arranged by size: 3536 1480 1197 552 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3625 487 Size: 683 56 2399 3685 Fragments arranged by size: 3685 2399 683 56 BtsI GCAGTG_nn' Cuts at: 2490 4339 5133 5153 2490 Size: 1849 794 20 4160 Fragments arranged by size: 4160 1849 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6823 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2827 2862 529 Size: 35 2263 35 4490 Fragments arranged by size: 4490 2263 35 35 DraI TTT'AAA Cuts at: 2707 4127 2707 Size: 1420 5403 DraIII CAC_nnn'GTG Cuts at: 3682 3682 Size: 6823 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5830 809 Size: 507 4514 1802 Fragments arranged by size: 4514 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2835 2880 3959 Size: 54 1425 50 275 18 1005 45 1079 Cuts at: 3959 4270 5159 6788 8 Size: 311 889 1629 43 Fragments arranged by size: 1629 1425 1079 1005 889 311 275 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3959 4270 1537 Size: 275 2147 311 4090 Fragments arranged by size: 4090 2147 311 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2200 3284 3770 3848 3857 4753 1353 Size: 847 1084 486 78 9 896 3423 Fragments arranged by size: 3423 1084 896 847 486 78 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3878 5410 5784 5930 387 Size: 1064 138 2289 1532 374 146 1280 Fragments arranged by size: 2289 1532 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2269 3763 2269 Size: 1494 5329 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2892 2920 3539 3830 4886 5483 6270 Size: 393 1260 28 619 291 1056 597 787 Cuts at: 6270 6538 1239 Size: 268 1524 Fragments arranged by size: 1524 1260 1056 787 619 597 393 291 268 28 EcoO109I rG'GnC_Cy Cuts at: 2049 2548 2751 3514 3826 2049 Size: 499 203 763 312 5046 Fragments arranged by size: 5046 763 499 312 203 EcoRI G'AATT_C Cuts at: 3197 4004 4132 4510 3197 Size: 807 128 378 5510 Fragments arranged by size: 5510 807 378 128 EcoRV GAT'ATC Cuts at: 4119 4119 Size: 6823 FspI TGC'GCA Cuts at: 5329 5329 Size: 6823 HaeII r_GCGC'y Cuts at: 1678 2440 2879 5970 1678 Size: 762 439 3091 2531 Fragments arranged by size: 3091 2531 762 439 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2234 2266 2628 Size: 32 135 32 461 32 169 32 362 Cuts at: 2628 2660 2694 2726 3677 3709 4035 4067 5464 Size: 32 34 32 951 32 326 32 1397 Cuts at: 5464 5496 5538 5570 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1397 951 461 362 326 169 135 42 34 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2080 4167 4413 669 Size: 988 281 142 2087 246 3079 Fragments arranged by size: 3079 2087 988 281 246 142 HindIII A'AGCT_T Cuts at: 748 748 Size: 6823 HpaI GTT'AAC Cuts at: 2080 4413 2080 Size: 2333 4490 KpnI G_GTAC'C Cuts at: 4148 4148 Size: 6823 MfeI C'AATT_G Cuts at: 4422 4422 Size: 6823 MluI A'CGCG_T Cuts at: 6699 6699 Size: 6823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 3033 5937 6121 1305 Size: 753 975 2904 184 2007 Fragments arranged by size: 2904 2007 975 753 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2837 2882 10 Size: 54 1425 343 1005 45 3951 Fragments arranged by size: 3951 1425 1005 343 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2807 3779 4781 5140 5299 518 Size: 648 1641 972 1002 359 159 2042 Fragments arranged by size: 2042 1641 1002 972 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4239 4916 6064 6309 1606 Size: 2633 677 1148 245 2120 Fragments arranged by size: 2633 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6271 NdeI CA'TA_TG Cuts at: 387 387 Size: 6823 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6823 NotI GC'GGCC_GC Cuts at: 4270 4270 Size: 6823 NsiI A_TGCA'T Cuts at: 2797 2797 Size: 6823 NspI r_CATG'y Cuts at: 1132 1229 2695 2799 3337 3595 4181 5726 1132 Size: 97 1466 104 538 258 586 1545 2229 Fragments arranged by size: 2229 1545 1466 586 538 258 104 97 PasI CC'CwG_GG Cuts at: 1348 2169 1348 Size: 821 6002 PciI A'CATG_T Cuts at: 3333 3591 5722 3333 Size: 258 2131 4434 Fragments arranged by size: 4434 2131 258 PflMI CCAn_nnn'nTGG Cuts at: 1108 3682 1108 Size: 2574 4249 PmeI GTTT'AAAC Cuts at: 4127 4127 Size: 6823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4892 4924 6405 6437 4892 Size: 32 1481 32 5278 Fragments arranged by size: 5278 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2049 2751 3514 3826 2049 Size: 702 763 312 5046 Fragments arranged by size: 5046 763 702 312 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6823 PsiI TTA'TAA Cuts at: 4393 4393 Size: 6823 PstI C_TGCA'G Cuts at: 830 3120 3483 3989 4175 830 Size: 2290 363 506 186 3478 Fragments arranged by size: 3478 2290 506 363 186 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6823 SacI G_AGCT'C Cuts at: 2271 3765 2271 Size: 1494 5329 SalI G'TCGA_C Cuts at: 1936 4165 1936 Size: 2229 4594 SanDI GG'GwC_CC Cuts at: 2751 2751 Size: 6823 SapI GCTCTTCn'nnn_ Cuts at: 3770 3848 3770 Size: 78 6745 SbfI CC_TGCA'GG Cuts at: 4175 4175 Size: 6823 ScaI AGT'ACT Cuts at: 5071 6783 5071 Size: 1712 5111 SfcI C'TryA_G Cuts at: 826 1045 3116 3479 3508 3985 4171 5306 5987 Size: 219 2071 363 29 477 186 1135 681 Cuts at: 5987 6178 826 Size: 191 1471 Fragments arranged by size: 2071 1471 1135 681 477 363 219 191 186 29 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6823 SmaI CCC'GGG Cuts at: 1872 4102 4150 1872 Size: 2230 48 4545 Fragments arranged by size: 4545 2230 48 SmlI C'TyrA_G Cuts at: 820 1017 1943 2337 2783 2914 4927 5828 6090 Size: 197 926 394 446 131 2013 901 262 Cuts at: 6090 820 Size: 1553 Fragments arranged by size: 2013 1553 926 901 446 394 262 197 131 SnaBI TAC'GTA Cuts at: 493 4614 493 Size: 4121 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6823 SphI G_CATG'C Cuts at: 1132 4181 1132 Size: 3049 3774 SspI AAT'ATT Cuts at: 5 52 4747 5 Size: 47 4695 2081 Fragments arranged by size: 4695 2081 47 StuI AGG'CCT Cuts at: 1517 3233 1517 Size: 1716 5107 StyI C'CwwG_G Cuts at: 513 1065 3303 3354 4262 513 Size: 552 2238 51 908 3074 Fragments arranged by size: 3074 2238 908 552 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4850 4867 5020 5205 5650 325 Size: 1255 3270 17 153 185 445 1498 Fragments arranged by size: 3270 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3341 5069 6781 Size: 274 80 33 51 1443 1364 1728 1712 Cuts at: 6781 96 Size: 138 Fragments arranged by size: 1728 1712 1443 1364 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3056 3088 6783 6815 3056 Size: 32 3695 32 3064 Fragments arranged by size: 3695 3064 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6823 XbaI T'CTAG_A Cuts at: 4159 4159 Size: 6823 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6823 XmaI C'CCGG_G Cuts at: 1870 4100 4148 1870 Size: 2230 48 4545 Fragments arranged by size: 4545 2230 48 XmnI GAAnn'nnTTC Cuts at: 3468 3617 3842 4073 4952 3468 Size: 149 225 231 879 5339 Fragments arranged by size: 5339 879 231 225 149 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4631 276 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI