(Circular) (Six-base) MAPSORT of: pFN21AB6188.seq Check: 7417 from: 1 to: 6814 pFN21AB6188 6814 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3816 3816 Size: 6814 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4624 278 Size: 53 83 186 936 3088 2468 Fragments arranged by size: 3088 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4157 1937 Size: 2220 4594 Acc65I G'GTAC_C Cuts at: 4135 4135 Size: 6814 AclI AA'CG_TT Cuts at: 1563 2454 4941 5314 1563 Size: 891 2487 373 3063 Fragments arranged by size: 3063 2487 891 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2243 2604 2901 2982 3002 3249 4877 6261 Size: 611 361 297 81 20 247 1628 1384 Cuts at: 6261 6529 1632 Size: 268 1917 Fragments arranged by size: 1917 1628 1384 611 361 297 268 247 81 20 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6617 AflIII A'CryG_T Cuts at: 1203 2045 5713 6690 1203 Size: 842 3668 977 1327 Fragments arranged by size: 3668 1327 977 842 AgeI A'CCGG_T Cuts at: 5645 5645 Size: 6814 AhdI GACnn_n'nnGTC Cuts at: 5543 5543 Size: 6814 AleI CACnn'nnGTG Cuts at: 1166 3962 1166 Size: 2796 4018 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2729 1184 Size: 1545 5269 AlwNI CAG_nnn'CTG Cuts at: 3061 4058 4557 6129 3061 Size: 997 499 1572 3746 Fragments arranged by size: 3746 1572 997 499 ApaI G_GGCC'C Cuts at: 2684 3386 3607 3910 2684 Size: 702 221 303 5588 Fragments arranged by size: 5588 702 303 221 ApaLI G'TGCA_C Cuts at: 4871 6027 4871 Size: 1156 5658 ApoI r'AATT_y Cuts at: 1493 4123 4349 4501 6610 1493 Size: 2630 226 152 2109 1697 Fragments arranged by size: 2630 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5368 160 Size: 872 4336 1606 Fragments arranged by size: 4336 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6814 AvaI C'yCGr_G Cuts at: 1870 1943 3910 4139 1870 Size: 73 1967 229 4545 Fragments arranged by size: 4545 1967 229 73 BamHI G'GATC_C Cuts at: 4144 4511 4144 Size: 367 6447 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2392 3452 4135 5590 618 Size: 325 217 627 605 1060 683 1455 1842 Fragments arranged by size: 1842 1455 1060 683 627 605 325 217 BanII G_rGCy'C Cuts at: 1391 2226 2684 3168 3386 3396 3607 3910 4133 Size: 835 458 484 218 10 211 303 223 Cuts at: 4133 1391 Size: 4072 Fragments arranged by size: 4072 835 484 458 303 223 218 211 10 BbeI G_GCGC'C Cuts at: 3456 3456 Size: 6814 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2384 2606 3202 3777 928 Size: 971 485 222 596 575 3965 Fragments arranged by size: 3965 971 596 575 485 222 BbvCI CC'TCA_GC Cuts at: 2303 2303 Size: 6814 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3698 3732 5005 5039 1903 Size: 34 1761 34 1273 34 3678 Fragments arranged by size: 3678 1761 1273 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4706 5916 4706 Size: 1210 5604 BclI T'GATC_A Cuts at: 1548 3000 1548 Size: 1452 5362 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2884 3149 4070 Size: 107 122 71 631 605 1212 265 921 Cuts at: 4070 4079 5425 136 Size: 9 1346 1525 Fragments arranged by size: 1525 1346 1212 921 631 605 265 122 107 71 9 BglII A'GATC_T Cuts at: 2254 6809 2254 Size: 4555 2259 BlpI GC'TnA_GC Cuts at: 2024 2069 4231 2024 Size: 45 2162 4607 Fragments arranged by size: 4607 2162 45 Bme1580I G_kGCm'C Cuts at: 1790 2684 3386 3607 3910 3993 4875 6031 1790 Size: 894 702 221 303 83 882 1156 2573 Fragments arranged by size: 2573 1156 894 882 702 303 221 83 BmgBI CAC'GTC Cuts at: 1318 2197 1318 Size: 879 5935 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2092 2828 2837 Size: 416 53 83 399 24 675 736 9 Cuts at: 2837 3414 4545 5503 442 Size: 577 1131 958 1753 Fragments arranged by size: 1753 1131 958 736 675 577 416 399 83 53 24 9 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6814 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2545 3083 3158 3350 3630 5474 1239 Size: 1306 538 75 192 280 1844 2579 Fragments arranged by size: 2579 1844 1306 538 280 192 75 Bpu10I CC'TnA_GC Cuts at: 2303 2303 Size: 6814 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3152 4939 5804 6102 3152 Size: 1787 865 298 3864 Fragments arranged by size: 3864 1787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3468 3881 5477 882 Size: 617 1969 413 1596 2219 Fragments arranged by size: 2219 1969 1596 617 413 BsaAI yAC'GTr Cuts at: 493 1204 4605 493 Size: 711 3401 2702 Fragments arranged by size: 3401 2702 711 BsaBI GATnn'nnATC Cuts at: 3935 3971 6808 3935 Size: 36 2837 3941 Fragments arranged by size: 3941 2837 36 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3453 4621 5003 5638 Size: 53 83 186 936 1920 1168 382 635 Cuts at: 5638 275 Size: 1451 Fragments arranged by size: 1920 1451 1168 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5247 5645 5919 6066 6477 5247 Size: 398 274 147 411 5584 Fragments arranged by size: 5584 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2171 2201 2391 2421 2571 Size: 30 165 30 768 30 190 30 150 Cuts at: 2571 2601 3824 3854 4067 4097 1178 Size: 30 1223 30 213 30 3895 Fragments arranged by size: 3895 1223 768 213 190 165 150 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 2287 2548 2597 2689 3270 3487 3938 Size: 961 140 261 49 92 581 217 451 Cuts at: 3938 1186 Size: 4062 Fragments arranged by size: 4062 961 581 451 261 217 140 92 49 BseYI C'CCAG_C Cuts at: 2644 2792 3322 3396 3401 3558 3817 6017 6513 Size: 148 530 74 5 157 259 2200 496 Cuts at: 6513 2644 Size: 2945 Fragments arranged by size: 2945 2200 530 496 259 157 148 74 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6241 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4264 5025 6053 1540 Size: 275 186 2263 761 1028 2301 Fragments arranged by size: 2301 2263 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2370 3168 4875 4960 6031 2370 Size: 798 1707 85 1071 3153 Fragments arranged by size: 3153 1707 1071 798 85 BsmI GAATG_Cn' Cuts at: 3121 3743 4325 4418 3121 Size: 622 582 93 5517 Fragments arranged by size: 5517 622 582 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2327 2327 Size: 6814 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2370 2684 3168 3386 3396 3607 Size: 399 436 144 314 484 218 10 211 Cuts at: 3607 3910 3993 4133 4875 4960 6031 1391 Size: 303 83 140 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 484 436 399 314 303 218 211 144 140 85 83 10 BspEI T'CCGG_A Cuts at: 6477 6477 Size: 6814 BspHI T'CATG_A Cuts at: 4703 6433 4703 Size: 1730 5084 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2073 3816 4169 844 Size: 1229 1743 353 3489 Fragments arranged by size: 3489 1743 1229 353 BsrBI CCG'CTC Cuts at: 1394 1823 2933 4701 1394 Size: 429 1110 1768 3507 Fragments arranged by size: 3507 1768 1110 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3222 4036 5309 5483 65 Size: 1183 1974 814 1273 174 1396 Fragments arranged by size: 1974 1396 1273 1183 814 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2887 3507 5458 5645 1700 Size: 110 1077 620 1951 187 2869 Fragments arranged by size: 2869 1951 1077 620 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6814 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6814 BssSI C'ACGA_G Cuts at: 4874 5886 4874 Size: 1012 5802 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6814 BstBI TT'CG_AA Cuts at: 5618 5618 Size: 6814 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6814 BstXI CCAn_nnnn'nTGG Cuts at: 3674 3674 Size: 6814 BstYI r'GATC_y Cuts at: 1972 2254 3591 4144 4511 4896 4913 6365 6451 Size: 282 1337 553 367 385 17 1452 86 Cuts at: 6451 6463 6809 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1337 553 385 367 346 282 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2701 3387 3533 2701 Size: 686 146 5982 Fragments arranged by size: 5982 686 146 BtgI C'CryG_G Cuts at: 513 1065 2004 2228 2903 3629 3873 3901 513 Size: 552 939 224 675 726 244 28 3426 Fragments arranged by size: 3426 939 726 675 552 244 224 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2301 487 Size: 683 56 1075 5000 Fragments arranged by size: 5000 1075 683 56 BtsI GCAGTG_nn' Cuts at: 2732 4330 5124 5144 2732 Size: 1598 794 20 4402 Fragments arranged by size: 4402 1598 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6814 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6779 DraI TTT'AAA Cuts at: 4118 4118 Size: 6814 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5821 809 Size: 507 4505 1802 Fragments arranged by size: 4505 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 4261 5150 Size: 54 1425 50 275 18 297 2134 889 Cuts at: 5150 6779 8 Size: 1629 43 Fragments arranged by size: 2134 1629 1425 889 297 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4261 1537 Size: 275 2449 4090 Fragments arranged by size: 4090 2449 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 2296 2912 3224 3494 4744 1353 Size: 800 143 616 312 270 1250 3423 Fragments arranged by size: 3423 1250 800 616 312 270 143 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3095 5401 5775 5921 387 Size: 1064 138 435 1071 2306 374 146 1280 Fragments arranged by size: 2306 1280 1071 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 3166 3166 Size: 6814 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2545 2604 2901 2982 3002 3083 Size: 393 611 302 59 297 81 20 81 Cuts at: 3083 3158 3249 3350 3630 4877 5474 6261 6529 Size: 75 91 101 280 1247 597 787 268 Cuts at: 6529 1239 Size: 1524 Fragments arranged by size: 1524 1247 787 611 597 393 302 297 280 268 101 91 81 81 75 59 20 EcoNI CCTnn'n_nnAGG Cuts at: 2855 2855 Size: 6814 EcoO109I rG'GnC_Cy Cuts at: 2587 3142 3372 3382 3383 3603 3906 3907 2587 Size: 555 230 10 1 220 303 1 5494 Fragments arranged by size: 5494 555 303 230 220 10 1 1 EcoRI G'AATT_C Cuts at: 4123 4501 4123 Size: 378 6436 FspI TGC'GCA Cuts at: 5320 5320 Size: 6814 HaeII r_GCGC'y Cuts at: 1678 3456 3571 5961 1678 Size: 1778 115 2390 2531 Fragments arranged by size: 2531 2390 1778 115 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2411 2443 2462 Size: 32 135 32 599 32 208 32 19 Cuts at: 2462 2494 2571 2597 2603 2629 2684 2716 3878 Size: 32 77 26 6 26 55 32 1162 Cuts at: 3878 3910 5455 5487 5529 5561 1373 Size: 32 1545 32 42 32 2626 Fragments arranged by size: 2626 1545 1162 599 208 135 77 55 42 32 32 32 32 32 32 32 32 32 26 26 19 6 HincII GTy'rAC Cuts at: 669 1657 1938 2527 4158 4404 669 Size: 988 281 589 1631 246 3079 Fragments arranged by size: 3079 1631 988 589 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6814 HpaI GTT'AAC Cuts at: 4404 4404 Size: 6814 KasI G'GCGC_C Cuts at: 3452 3452 Size: 6814 KpnI G_GTAC'C Cuts at: 4139 4139 Size: 6814 MfeI C'AATT_G Cuts at: 3756 4413 3756 Size: 657 6157 MluI A'CGCG_T Cuts at: 2045 6690 2045 Size: 4645 2169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3691 5928 6112 1305 Size: 2386 2237 184 2007 Fragments arranged by size: 2386 2237 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4992 Fragments arranged by size: 4992 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3962 4772 5131 5290 518 Size: 648 2796 810 359 159 2042 Fragments arranged by size: 2796 2042 810 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3706 4230 4907 6055 6300 1606 Size: 2100 524 677 1148 245 2120 Fragments arranged by size: 2120 2100 1148 677 524 245 NaeI GCC'GGC Cuts at: 2889 3509 2889 Size: 620 6194 NarI GG'CG_CC Cuts at: 3453 3453 Size: 6814 NcoI C'CATG_G Cuts at: 513 1065 2004 2228 3873 513 Size: 552 939 224 1645 3454 Fragments arranged by size: 3454 1645 939 552 224 NdeI CA'TA_TG Cuts at: 387 387 Size: 6814 NgoMIV G'CCGG_C Cuts at: 2887 3507 2887 Size: 620 6194 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6814 NotI GC'GGCC_GC Cuts at: 4261 4261 Size: 6814 NspI r_CATG'y Cuts at: 1132 1229 4172 5717 1132 Size: 97 2943 1545 2229 Fragments arranged by size: 2943 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3379 3446 1348 Size: 2031 67 4716 Fragments arranged by size: 4716 2031 67 PciI A'CATG_T Cuts at: 5713 5713 Size: 6814 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6814 PfoI T'CCnGG_A Cuts at: 2589 3029 2589 Size: 440 6374 PmeI GTTT'AAAC Cuts at: 4118 4118 Size: 6814 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4883 4915 6396 6428 4883 Size: 32 1481 32 5269 Fragments arranged by size: 5269 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2587 3372 2587 Size: 785 6029 PshAI GACnn'nnGTC Cuts at: 1613 2085 3619 1613 Size: 472 1534 4808 Fragments arranged by size: 4808 1534 472 PsiI TTA'TAA Cuts at: 4384 4384 Size: 6814 PspOMI G'GGCC_C Cuts at: 2680 3382 3603 3906 2680 Size: 702 221 303 5588 Fragments arranged by size: 5588 702 303 221 PstI C_TGCA'G Cuts at: 830 2094 2739 2814 3057 4063 4166 830 Size: 1264 645 75 243 1006 103 3478 Fragments arranged by size: 3478 1264 1006 645 243 103 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6814 PvuII CAG'CTG Cuts at: 3706 3706 Size: 6814 SacI G_AGCT'C Cuts at: 3168 3168 Size: 6814 SalI G'TCGA_C Cuts at: 1936 4156 1936 Size: 2220 4594 SapI GCTCTTCn'nnn_ Cuts at: 2153 2296 2912 3494 2153 Size: 143 616 582 5473 Fragments arranged by size: 5473 616 582 143 SbfI CC_TGCA'GG Cuts at: 4166 4166 Size: 6814 ScaI AGT'ACT Cuts at: 3084 5062 6774 3084 Size: 1978 1712 3124 Fragments arranged by size: 3124 1978 1712 SfcI C'TryA_G Cuts at: 826 1045 2090 2735 2748 2810 3053 4059 4162 Size: 219 1045 645 13 62 243 1006 103 Cuts at: 4162 5297 5978 6169 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1045 1006 681 645 243 219 191 103 62 13 SfiI GGCCn_nnn'nGGCC Cuts at: 4079 4079 Size: 6814 SfoI GGC'GCC Cuts at: 3454 3454 Size: 6814 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6814 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6814 SmaI CCC'GGG Cuts at: 1872 3912 4141 1872 Size: 2040 229 4545 Fragments arranged by size: 4545 2040 229 SmlI C'TyrA_G Cuts at: 820 1017 1943 3167 4918 5819 6081 820 Size: 197 926 1224 1751 901 262 1553 Fragments arranged by size: 1751 1553 1224 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4605 493 Size: 4112 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6814 SphI G_CATG'C Cuts at: 1132 4172 1132 Size: 3040 3774 SspI AAT'ATT Cuts at: 5 52 4738 5 Size: 47 4686 2081 Fragments arranged by size: 4686 2081 47 StuI AGG'CCT Cuts at: 1517 2537 4082 1517 Size: 1020 1545 4249 Fragments arranged by size: 4249 1545 1020 StyI C'CwwG_G Cuts at: 513 1065 2004 2228 2388 3456 3873 4253 513 Size: 552 939 224 160 1068 417 380 3074 Fragments arranged by size: 3074 1068 939 552 417 380 224 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3738 3777 4841 4858 5011 5196 5641 Size: 1255 2158 39 1064 17 153 185 445 Cuts at: 5641 325 Size: 1498 Fragments arranged by size: 2158 1498 1255 1064 445 185 153 39 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3082 5060 6772 Size: 274 80 33 51 1443 1105 1978 1712 Cuts at: 6772 96 Size: 138 Fragments arranged by size: 1978 1712 1443 1105 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2391 2423 4067 4099 6774 6806 2391 Size: 32 1644 32 2675 32 2399 Fragments arranged by size: 2675 2399 1644 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2383 2605 3892 1316 Size: 1067 222 1287 4238 Fragments arranged by size: 4238 1287 1067 222 XbaI T'CTAG_A Cuts at: 4150 4150 Size: 6814 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3856 3856 Size: 6814 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6814 XmaI C'CCGG_G Cuts at: 1870 3910 4139 1870 Size: 2040 229 4545 Fragments arranged by size: 4545 2040 229 XmnI GAAnn'nnTTC Cuts at: 4943 4943 Size: 6814 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4622 276 Size: 53 83 186 936 3088 2468 Fragments arranged by size: 3088 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI