Restriction map for
pFN21AB6174
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbrPI
BcgI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsiEI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 52 CGCG CG^CG
AccIII 647 TCCGGA T^CCGGA
AclI 453 AACGTT AA^CGTT
AcsI 1156 AAATTC [GA]^AATT[CT]
ApoI 1156 AAATTC [GA]^AATT[CT]
Asp700I 55 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 333 CCCGAG C^[CT]CG[GA]G
BalI 711 TGGCCA TGG^CCA
BamHI 125 GGATCC G^GATCC
BbsI 556 GAAGAC GAAGAC(2/6)
BbvCI 4 GCTGAGG CCTCAGC(-5/-2)
BciVI 1584 GGATAC GTATCC(6/5)
BclI 1790 TGATCA T^GATCA
BpmI 1874 CTGGAG CTGGAG(16/14)
BpuAI 556 GAAGAC GAAGAC(2/6)
BsaWI 647 TCCGGA [AT]^CCGG[AT]
BseAI 647 TCCGGA T^CCGGA
BsmBI 636 GAGACG CGTCTC(1/5)
BsmFI 963 GTCCC GGGAC(10/14)
BsoBI 333 CCCGAG C^[CT]CG[GA]G
BspEI 647 TCCGGA T^CCGGA
BsrDI 715 CATTGC GCAATG(2/0)
BsrFI 1070 GCCGGC [GA]^CCGG[CT]
BstAPI 200 GCATCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 52 CGCG CG^CG
BstYI 125 GGATCC [GA]^GATC[CT]
Cfr10I 1070 GCCGGC [GA]^CCGG[CT]
DsaI 233 CCGTGG C^C[GA][CT]GG
EcoNI 1512 CCTGTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1790 TGATCA T^GATCA
MflI 125 GGATCC [GA]^GATC[CT]
MluNI 711 TGGCCA TGG^CCA
MroI 647 TCCGGA T^CCGGA
MscI 711 TGGCCA TGG^CCA
MslI 1782 CACACCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 52 CGCG CG^CG
NaeI 1070 GCCGGC GCC^GGC
NgoMIV 1070 GCCGGC G^CCGGC
NspI 873 ACATGC [GA]CATG^[CT]
Psp1406I 453 AACGTT AA^CGTT
StuI 1671 AGGCCT AGG^CCT
Tsp45I 896 GTGAC ^GT[GC]AC
XcmI 699 CCAAGAGGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 125 GGATCC [GA]^GATC[CT]
XmnI 55 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1036 GTAGAC GT^[AC][GT]AC
1663 GTAGAC
AspHI 473 GAGCAC G[AT]GC[AT]^C
1530 GTGCTC
AvaII 301 GGACC G^G[AT]CC
1853 GGTCC
BfaI 1652 CTAG C^TAG
1880 CTAG
BglI 217 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
264 GCCTTCAAGGC
BsaI 343 GAGACC GGTCTC(1/5)
748 GGTCTC
BseRI 1708 GAGGAG GAGGAG(10/8)
1720 GAGGAG
BsiHKAI 473 GAGCAC G[AT]GC[AT]^C
1530 GTGCTC
BstXI 989 CCAGAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1527 CCAGTGCTCTGG
EaeI 711 TGGCCA [CT]^GGCC[GA]
1125 TGGCCG
EarI 181 CTCTTC CTCTTC(1/4)
1762 GAAGAG
Ksp632I 181 CTCTTC CTCTTC(1/4)
1762 GAAGAG
MaeI 1652 CTAG C^TAG
1880 CTAG
MaeIII 550 GTAAC ^GT[ACGT]AC
896 GTGAC
MseI 512 TTAA T^TAA
1865 TTAA
PstI 586 CTGCAG CTGCA^G
1207 CTGCAG
PvuII 589 CAGCTG CAG^CTG
1613 CAGCTG
SfaNI 200 GCATC GCATC(5/9)
607 GCATC
TfiI 410 GAATC G^A[AT]TC
1735 GATTC
Tru9I 512 TTAA T^TAA
1865 TTAA
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
ApaI BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BsgI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NarI NciI NdeII NlaIII
NlaIV PflMI PleI Sau3AI Sau96I
ScrFI SfcI SfoI StyI TaqI
TseI Tsp509I TspRI TthHB8I Van91I