(Circular) (Six-base) MAPSORT of: pFN21AB6169.seq Check: 324 from: 1 to: 6655 pFN21AB6169 6655 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4465 278 Size: 53 83 186 936 2929 2468 Fragments arranged by size: 2929 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2439 3998 1937 Size: 502 1559 4594 Fragments arranged by size: 4594 1559 502 Acc65I G'GTAC_C Cuts at: 3057 3976 3057 Size: 919 5736 AclI AA'CG_TT Cuts at: 1563 4782 5155 1563 Size: 3219 373 3063 Fragments arranged by size: 3219 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3180 3652 4718 6102 6370 1632 Size: 1548 472 1066 1384 268 1917 Fragments arranged by size: 1917 1548 1384 1066 472 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6458 AflIII A'CryG_T Cuts at: 1203 5554 6531 1203 Size: 4351 977 1327 Fragments arranged by size: 4351 1327 977 AgeI A'CCGG_T Cuts at: 5486 5486 Size: 6655 AhdI GACnn_n'nnGTC Cuts at: 3670 3700 5384 3670 Size: 30 1684 4941 Fragments arranged by size: 4941 1684 30 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6655 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6655 AlwNI CAG_nnn'CTG Cuts at: 2261 4398 5970 2261 Size: 2137 1572 2946 Fragments arranged by size: 2946 2137 1572 ApaI G_GGCC'C Cuts at: 2772 3044 3353 3478 2772 Size: 272 309 125 5949 Fragments arranged by size: 5949 309 272 125 ApaLI G'TGCA_C Cuts at: 2063 2251 4712 5868 2063 Size: 188 2461 1156 2850 Fragments arranged by size: 2850 2461 1156 188 ApoI r'AATT_y Cuts at: 1493 3964 4190 4342 6451 1493 Size: 2471 226 152 2109 1697 Fragments arranged by size: 2471 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5209 160 Size: 872 4177 1606 Fragments arranged by size: 4177 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6655 AvaI C'yCGr_G Cuts at: 1870 1943 2918 3345 3471 3625 3645 3675 3720 Size: 73 975 427 126 154 20 30 45 Cuts at: 3720 3729 3900 3980 1870 Size: 9 171 80 4545 Fragments arranged by size: 4545 975 427 171 154 126 80 73 45 30 20 9 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3700 3733 3700 Size: 33 6622 BamHI G'GATC_C Cuts at: 3985 4352 3985 Size: 367 6288 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2847 2886 3057 3976 5431 Size: 325 217 627 1060 39 171 919 1455 Cuts at: 5431 618 Size: 1842 Fragments arranged by size: 1842 1455 1060 919 627 325 217 171 39 BanII G_rGCy'C Cuts at: 1391 2172 2189 2712 2748 2772 3044 3353 3429 Size: 781 17 523 36 24 272 309 76 Cuts at: 3429 3478 3974 1391 Size: 49 496 4072 Fragments arranged by size: 4072 781 523 496 309 272 76 49 36 24 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2215 2784 928 Size: 971 316 569 4799 Fragments arranged by size: 4799 971 569 316 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4846 4880 1903 Size: 34 50 34 2825 34 3678 Fragments arranged by size: 3678 2825 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3062 4547 5757 3062 Size: 1485 1210 3960 Fragments arranged by size: 3960 1485 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6655 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2141 2150 2462 Size: 107 122 71 631 605 469 9 312 Cuts at: 2462 5266 136 Size: 2804 1525 Fragments arranged by size: 2804 1525 631 605 469 312 122 107 71 9 BglII A'GATC_T Cuts at: 6650 6650 Size: 6655 BlpI GC'TnA_GC Cuts at: 2240 2742 3097 4072 2240 Size: 502 355 975 4823 Fragments arranged by size: 4823 975 502 355 Bme1580I G_kGCm'C Cuts at: 1790 2067 2255 2772 2891 3044 3353 3478 4716 Size: 277 188 517 119 153 309 125 1238 Cuts at: 4716 5872 1790 Size: 1156 2573 Fragments arranged by size: 2573 1238 1156 517 309 277 188 153 125 119 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6655 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3267 4386 5344 Size: 416 53 83 399 24 1850 1119 958 Cuts at: 5344 442 Size: 1753 Fragments arranged by size: 1850 1753 1119 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6655 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3555 3904 3555 Size: 349 6306 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2158 2666 3084 3096 3727 3800 5315 1239 Size: 919 508 418 12 631 73 1515 2579 Fragments arranged by size: 2579 1515 919 631 508 418 73 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3899 4780 5645 5943 3899 Size: 881 865 298 4611 Fragments arranged by size: 4611 881 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2671 3543 3678 3814 3875 5318 882 Size: 617 1172 872 135 136 61 1443 2219 Fragments arranged by size: 2219 1443 1172 872 617 136 135 61 BsaAI yAC'GTr Cuts at: 493 1204 2302 4446 493 Size: 711 1098 2144 2702 Fragments arranged by size: 2702 2144 1098 711 BsaBI GATnn'nnATC Cuts at: 2591 6649 2591 Size: 4058 2597 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3489 3896 4462 4844 Size: 53 83 186 936 1956 407 566 382 Cuts at: 4844 5479 275 Size: 635 1451 Fragments arranged by size: 1956 1451 936 635 566 407 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2597 5088 5486 5760 5907 6318 2597 Size: 2491 398 274 147 411 2934 Fragments arranged by size: 2934 2491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3725 3755 1178 Size: 30 165 30 2322 30 4078 Fragments arranged by size: 4078 2322 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2071 2819 3392 3574 3797 3824 3829 3832 Size: 885 748 573 182 223 27 5 3 Cuts at: 3832 3848 3851 1186 Size: 16 3 3990 Fragments arranged by size: 3990 885 748 573 223 182 27 16 5 3 3 BseYI C'CCAG_C Cuts at: 2182 2457 2963 2978 5858 6354 2182 Size: 275 506 15 2880 496 2483 Fragments arranged by size: 2880 2483 506 496 275 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6082 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2145 2199 2220 4105 4866 5894 Size: 275 186 144 54 21 1885 761 1028 Cuts at: 5894 1540 Size: 2301 Fragments arranged by size: 2301 1885 1028 761 275 186 144 54 21 BsiHKAI G_wGCw'C Cuts at: 2067 2255 2712 2748 3518 4716 4801 5872 2067 Size: 188 457 36 770 1198 85 1071 2850 Fragments arranged by size: 2850 1198 1071 770 457 188 85 36 BsmI GAATG_Cn' Cuts at: 4166 4259 4166 Size: 93 6562 BsmBI CGTCTCn'nnnn_ Cuts at: 3072 3072 Size: 6655 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2172 2189 2255 2712 2748 2772 Size: 399 277 105 17 66 457 36 24 Cuts at: 2772 2891 3044 3353 3429 3478 3518 3974 4716 Size: 119 153 309 76 49 40 456 742 Cuts at: 4716 4801 5872 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 457 456 399 309 277 153 119 105 85 76 66 49 40 36 24 17 BspEI T'CCGG_A Cuts at: 6318 6318 Size: 6655 BspHI T'CATG_A Cuts at: 4544 6274 4544 Size: 1730 4925 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3525 4010 844 Size: 2681 485 3489 Fragments arranged by size: 3489 2681 485 BsrBI CCG'CTC Cuts at: 1394 1823 3289 3480 4542 1394 Size: 429 1466 191 1062 3507 Fragments arranged by size: 3507 1466 1062 429 191 BsrDI GCAATG_nn' Cuts at: 65 1248 5150 5324 65 Size: 1183 3902 174 1396 Fragments arranged by size: 3902 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2162 2936 5299 5486 1700 Size: 110 352 774 2363 187 2869 Fragments arranged by size: 2869 2363 774 352 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6655 BssHII G'CGCG_C Cuts at: 1925 2085 1925 Size: 160 6495 BssSI C'ACGA_G Cuts at: 3317 3544 4715 5727 3317 Size: 227 1171 1012 4245 Fragments arranged by size: 4245 1171 1012 227 BstAPI GCAn_nnn'nTGC Cuts at: 794 2250 794 Size: 1456 5199 BstBI TT'CG_AA Cuts at: 5459 5459 Size: 6655 BstEII G'GTnAC_C Cuts at: 1184 2278 1184 Size: 1094 5561 BstXI CCAn_nnnn'nTGG Cuts at: 2182 2182 Size: 6655 BstYI r'GATC_y Cuts at: 1972 3985 4352 4737 4754 6206 6292 6304 6650 Size: 2013 367 385 17 1452 86 12 346 Cuts at: 6650 1972 Size: 1977 Fragments arranged by size: 2013 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2099 2731 2953 2099 Size: 632 222 5801 Fragments arranged by size: 5801 632 222 BtgI C'CryG_G Cuts at: 513 1065 2661 3036 3483 3584 513 Size: 552 1596 375 447 101 3584 Fragments arranged by size: 3584 1596 552 447 375 101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2162 2395 487 Size: 683 56 936 233 4747 Fragments arranged by size: 4747 936 683 233 56 BtsI GCAGTG_nn' Cuts at: 3154 3175 4171 4965 4985 3154 Size: 21 996 794 20 4824 Fragments arranged by size: 4824 996 794 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6655 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6620 DraI TTT'AAA Cuts at: 3959 3959 Size: 6655 DraIII CAC_nnn'GTG Cuts at: 2250 2305 2250 Size: 55 6600 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2219 5662 809 Size: 507 903 3443 1802 Fragments arranged by size: 3443 1802 903 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2160 2196 Size: 54 1425 50 275 18 312 18 36 Cuts at: 2196 2430 2532 3106 3240 3277 3388 4102 4991 Size: 234 102 574 134 37 111 714 889 Cuts at: 4991 6620 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 714 574 312 275 234 134 111 102 54 50 43 37 36 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2142 2196 4102 1537 Size: 275 330 54 1906 4090 Fragments arranged by size: 4090 1906 330 275 54 EarI CTCTTCn'nnn_ Cuts at: 1353 2318 2792 3837 4585 1353 Size: 965 474 1045 748 3423 Fragments arranged by size: 3423 1045 965 748 474 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5242 5616 5762 387 Size: 1064 138 3653 374 146 1280 Fragments arranged by size: 3653 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2710 2746 2710 Size: 36 6619 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2158 2666 3084 3096 3180 3652 3727 Size: 393 526 508 418 12 84 472 75 Cuts at: 3727 3800 4718 5315 6102 6370 1239 Size: 73 918 597 787 268 1524 Fragments arranged by size: 1524 918 787 597 526 508 472 418 393 268 84 75 73 12 EcoNI CCTnn'n_nnAGG Cuts at: 3317 3317 Size: 6655 EcoO109I rG'GnC_Cy Cuts at: 2677 2727 2768 2923 3041 3214 3349 3350 3649 Size: 50 41 155 118 173 135 1 299 Cuts at: 3649 3702 3724 3754 2677 Size: 53 22 30 5578 Fragments arranged by size: 5578 299 173 155 135 118 53 50 41 30 22 1 EcoRI G'AATT_C Cuts at: 3964 4342 3964 Size: 378 6277 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3940 3940 Size: 6655 FspI TGC'GCA Cuts at: 2516 5161 2516 Size: 2645 4010 FspAI rTGC'GCAy Cuts at: 2516 2516 Size: 6655 HaeII r_GCGC'y Cuts at: 1678 2156 3685 5802 1678 Size: 478 1529 2117 2531 Fragments arranged by size: 2531 2117 1529 478 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2408 2440 3317 3349 3656 Size: 32 135 32 836 32 877 32 307 Cuts at: 3656 3686 3688 3718 3725 3757 5296 5328 5370 Size: 30 2 30 7 32 1539 32 42 Cuts at: 5370 5402 1373 Size: 32 2626 Fragments arranged by size: 2626 1539 877 836 307 135 42 32 32 32 32 32 32 32 30 30 7 2 HincII GTy'rAC Cuts at: 669 1657 1938 2440 3085 3999 4245 669 Size: 988 281 502 645 914 246 3079 Fragments arranged by size: 3079 988 914 645 502 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6655 HpaI GTT'AAC Cuts at: 4245 4245 Size: 6655 KpnI G_GTAC'C Cuts at: 3061 3980 3061 Size: 919 5736 MfeI C'AATT_G Cuts at: 4254 4254 Size: 6655 MluI A'CGCG_T Cuts at: 6531 6531 Size: 6655 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2313 3786 5769 5953 1305 Size: 1008 1473 1983 184 2007 Fragments arranged by size: 2007 1983 1473 1008 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2534 3108 3242 3390 10 Size: 54 1425 343 702 574 134 148 3275 Fragments arranged by size: 3275 1425 702 574 343 148 134 54 MslI CAynn'nnrTG Cuts at: 518 1166 2276 4613 4972 5131 518 Size: 648 1110 2337 359 159 2042 Fragments arranged by size: 2337 2042 1110 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2461 3101 3586 4071 4748 5896 6141 1606 Size: 855 640 485 485 677 1148 245 2120 Fragments arranged by size: 2120 1148 855 677 640 485 485 245 NaeI GCC'GGC Cuts at: 2164 2938 2164 Size: 774 5881 NcoI C'CATG_G Cuts at: 513 1065 3036 513 Size: 552 1971 4132 Fragments arranged by size: 4132 1971 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6655 NgoMIV G'CCGG_C Cuts at: 2162 2936 2162 Size: 774 5881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6655 NotI GC'GGCC_GC Cuts at: 4102 4102 Size: 6655 NspI r_CATG'y Cuts at: 1132 1229 3580 4013 5558 1132 Size: 97 2351 433 1545 2229 Fragments arranged by size: 2351 2229 1545 433 97 PasI CC'CwG_GG Cuts at: 1348 2560 3408 1348 Size: 1212 848 4595 Fragments arranged by size: 4595 1212 848 PciI A'CATG_T Cuts at: 5554 5554 Size: 6655 PflMI CCAn_nnn'nTGG Cuts at: 1108 3414 1108 Size: 2306 4349 PfoI T'CCnGG_A Cuts at: 3369 3369 Size: 6655 PmeI GTTT'AAAC Cuts at: 3959 3959 Size: 6655 PmlI CAC'GTG Cuts at: 2302 2302 Size: 6655 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4724 4756 6237 6269 4724 Size: 32 1481 32 5110 Fragments arranged by size: 5110 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2677 2727 3214 3649 3702 3724 3754 2677 Size: 50 487 435 53 22 30 5578 Fragments arranged by size: 5578 487 435 53 50 30 22 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6655 PsiI TTA'TAA Cuts at: 4225 4225 Size: 6655 PspOMI G'GGCC_C Cuts at: 2768 3040 3349 3474 2768 Size: 272 309 125 5949 Fragments arranged by size: 5949 309 272 125 PspXI vC'TCGA_Gb Cuts at: 3625 3625 Size: 6655 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2465 2497 2465 Size: 32 6623 PstI C_TGCA'G Cuts at: 830 4007 830 Size: 3177 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6655 PvuII CAG'CTG Cuts at: 2461 3101 2461 Size: 640 6015 SacI G_AGCT'C Cuts at: 2712 2748 2712 Size: 36 6619 SacII CC_GC'GG Cuts at: 3587 3587 Size: 6655 SalI G'TCGA_C Cuts at: 1936 2438 3997 1936 Size: 502 1559 4594 Fragments arranged by size: 4594 1559 502 SanDI GG'GwC_CC Cuts at: 3649 3724 3649 Size: 75 6580 SapI GCTCTTCn'nnn_ Cuts at: 2792 2792 Size: 6655 SbfI CC_TGCA'GG Cuts at: 4007 4007 Size: 6655 ScaI AGT'ACT Cuts at: 4903 6615 4903 Size: 1712 4943 SexAI A'CCwGG_T Cuts at: 2246 2752 2246 Size: 506 6149 SfcI C'TryA_G Cuts at: 826 1045 3175 4003 5138 5819 6010 826 Size: 219 2130 828 1135 681 191 1471 Fragments arranged by size: 2130 1471 1135 828 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6655 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6655 SmaI CCC'GGG Cuts at: 1872 3347 3473 3647 3722 3982 1872 Size: 1475 126 174 75 260 4545 Fragments arranged by size: 4545 1475 260 174 126 75 SmlI C'TyrA_G Cuts at: 820 1017 1943 3625 3914 4759 5660 5922 820 Size: 197 926 1682 289 845 901 262 1553 Fragments arranged by size: 1682 1553 926 901 845 289 262 197 SnaBI TAC'GTA Cuts at: 493 4446 493 Size: 3953 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6655 SphI G_CATG'C Cuts at: 1132 4013 1132 Size: 2881 3774 SspI AAT'ATT Cuts at: 5 52 4579 5 Size: 47 4527 2081 Fragments arranged by size: 4527 2081 47 StuI AGG'CCT Cuts at: 1517 2699 3848 1517 Size: 1182 1149 4324 Fragments arranged by size: 4324 1182 1149 StyI C'CwwG_G Cuts at: 513 1065 2078 2719 3036 3109 4094 513 Size: 552 1013 641 317 73 985 3074 Fragments arranged by size: 3074 1013 985 641 552 317 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3899 4682 4699 4852 5037 5482 325 Size: 1255 2319 783 17 153 185 445 1498 Fragments arranged by size: 2319 1498 1255 783 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2385 3219 4901 Size: 274 80 33 51 1443 408 834 1682 Cuts at: 4901 6613 96 Size: 1712 138 Fragments arranged by size: 1712 1682 1443 834 408 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2029 2061 3365 3397 6615 6647 2029 Size: 32 1304 32 3218 32 2037 Fragments arranged by size: 3218 2037 1304 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2216 2284 1316 Size: 900 68 5687 Fragments arranged by size: 5687 900 68 XbaI T'CTAG_A Cuts at: 3991 3991 Size: 6655 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3280 3280 Size: 6655 XhoI C'TCGA_G Cuts at: 1943 3625 1943 Size: 1682 4973 XmaI C'CCGG_G Cuts at: 1870 3345 3471 3645 3720 3980 1870 Size: 1475 126 174 75 260 4545 Fragments arranged by size: 4545 1475 260 174 126 75 XmnI GAAnn'nnTTC Cuts at: 4784 4784 Size: 6655 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4463 276 Size: 53 83 186 936 2929 2468 Fragments arranged by size: 2929 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoRV FseI KasI NarI NruI NsiI PacI RsrII SfiI SfoI SrfI SwaI