(Circular) (Six-base) MAPSORT of: pFN21AB6148.seq Check: 4998 from: 1 to: 6625 pFN21AB6148 6625 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4435 278 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3968 1937 Size: 2031 4594 Acc65I G'GTAC_C Cuts at: 3946 3946 Size: 6625 AclI AA'CG_TT Cuts at: 1563 4752 5125 1563 Size: 3189 373 3063 Fragments arranged by size: 3189 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2672 3549 4688 6072 6340 1632 Size: 1040 877 1139 1384 268 1917 Fragments arranged by size: 1917 1384 1139 1040 877 268 AfeI AGC'GCT Cuts at: 2045 2045 Size: 6625 AflII C'TTAA_G Cuts at: 820 1017 3473 820 Size: 197 2456 3972 Fragments arranged by size: 3972 2456 197 AflIII A'CryG_T Cuts at: 1203 5524 6501 1203 Size: 4321 977 1327 Fragments arranged by size: 4321 1327 977 AgeI A'CCGG_T Cuts at: 5456 5456 Size: 6625 AhdI GACnn_n'nnGTC Cuts at: 2845 5354 2845 Size: 2509 4116 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6625 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6625 AlwNI CAG_nnn'CTG Cuts at: 2537 4368 5940 2537 Size: 1831 1572 3222 Fragments arranged by size: 3222 1831 1572 ApaLI G'TGCA_C Cuts at: 4682 5838 4682 Size: 1156 5469 ApoI r'AATT_y Cuts at: 1493 2152 2208 2499 2758 3181 3517 3893 3934 Size: 659 56 291 259 423 336 376 41 Cuts at: 3934 4160 4312 6421 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 659 423 376 336 291 259 226 152 56 41 AseI AT'TA_AT Cuts at: 160 1032 5179 160 Size: 872 4147 1606 Fragments arranged by size: 4147 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 AvaI C'yCGr_G Cuts at: 1870 1943 2129 3950 1870 Size: 73 186 1821 4545 Fragments arranged by size: 4545 1821 186 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3247 3280 3247 Size: 33 6592 BamHI G'GATC_C Cuts at: 3955 4322 3955 Size: 367 6258 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2900 3946 5401 618 Size: 325 217 627 1113 1046 1455 1842 Fragments arranged by size: 1842 1455 1113 1046 627 325 217 BanII G_rGCy'C Cuts at: 1391 3944 1391 Size: 2553 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5654 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4816 4850 1903 Size: 34 2879 34 3678 Fragments arranged by size: 3678 2879 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2819 4517 5727 2819 Size: 1698 1210 3717 Fragments arranged by size: 3717 1698 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6625 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2654 5236 Size: 107 122 71 631 605 337 645 2582 Cuts at: 5236 136 Size: 1525 Fragments arranged by size: 2582 1525 645 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3469 6620 3469 Size: 3151 3474 BlpI GC'TnA_GC Cuts at: 4042 4042 Size: 6625 Bme1580I G_kGCm'C Cuts at: 1790 4686 5842 1790 Size: 2896 1156 2573 Fragments arranged by size: 2896 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6625 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4356 5314 442 Size: 416 53 83 399 24 2939 958 1753 Fragments arranged by size: 2939 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6625 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2302 2346 2672 3192 3493 3729 5285 1239 Size: 1063 44 326 520 301 236 1556 2579 Fragments arranged by size: 2579 1556 1063 520 326 301 236 44 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2499 3210 4750 5615 5913 2499 Size: 711 1540 865 298 3211 Fragments arranged by size: 3211 1540 865 711 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5288 882 Size: 617 3789 2219 Fragments arranged by size: 3789 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4416 493 Size: 711 3212 2702 Fragments arranged by size: 3212 2702 711 BsaBI GATnn'nnATC Cuts at: 6619 6619 Size: 6625 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4432 4814 5449 275 Size: 53 83 186 936 2899 382 635 1451 Fragments arranged by size: 2899 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2668 5058 5456 5730 5877 6288 2668 Size: 2390 398 274 147 411 3005 Fragments arranged by size: 3005 2390 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2705 2735 3179 3209 1178 Size: 30 165 30 1302 30 444 30 4594 Fragments arranged by size: 4594 1302 444 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2444 2943 3159 3543 1186 Size: 865 393 499 216 384 4268 Fragments arranged by size: 4268 865 499 393 384 216 BseYI C'CCAG_C Cuts at: 2068 2334 5828 6324 2068 Size: 266 3494 496 2369 Fragments arranged by size: 3494 2369 496 266 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6052 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4075 4836 5864 1540 Size: 275 186 2074 761 1028 2301 Fragments arranged by size: 2301 2074 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2865 4686 4771 5842 2865 Size: 1821 85 1071 3648 Fragments arranged by size: 3648 1821 1071 85 BsmI GAATG_Cn' Cuts at: 2896 4136 4229 2896 Size: 1240 93 5292 Fragments arranged by size: 5292 1240 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2105 2889 2931 3616 2105 Size: 784 42 685 5114 Fragments arranged by size: 5114 784 685 42 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2865 3944 4686 4771 5842 1391 Size: 399 1075 1079 742 85 1071 2174 Fragments arranged by size: 2174 1079 1075 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6288 6288 Size: 6625 BspHI T'CATG_A Cuts at: 3772 4514 6244 3772 Size: 742 1730 4153 Fragments arranged by size: 4153 1730 742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3980 844 Size: 3136 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4512 1394 Size: 429 2689 3507 Fragments arranged by size: 3507 2689 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5120 5294 65 Size: 1183 3872 174 1396 Fragments arranged by size: 3872 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5269 5456 1700 Size: 110 3459 187 2869 Fragments arranged by size: 3459 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6625 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6625 BssSI C'ACGA_G Cuts at: 2406 4685 5697 2406 Size: 2279 1012 3334 Fragments arranged by size: 3334 2279 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6625 BstBI TT'CG_AA Cuts at: 2581 5429 2581 Size: 2848 3777 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6625 BstXI CCAn_nnnn'nTGG Cuts at: 2696 2696 Size: 6625 BstYI r'GATC_y Cuts at: 1972 3469 3955 4322 4707 4724 6176 6262 6274 Size: 1497 486 367 385 17 1452 86 12 Cuts at: 6274 6620 1972 Size: 346 1977 Fragments arranged by size: 1977 1497 1452 486 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2283 2283 Size: 6625 BtgI C'CryG_G Cuts at: 513 1065 2004 2194 3555 513 Size: 552 939 190 1361 3583 Fragments arranged by size: 3583 1361 939 552 190 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5886 Fragments arranged by size: 5886 683 56 BtsI GCAGTG_nn' Cuts at: 3546 4141 4935 4955 3546 Size: 595 794 20 5216 Fragments arranged by size: 5216 794 595 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6625 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2452 2487 529 Size: 35 1888 35 4667 Fragments arranged by size: 4667 1888 35 35 DraI TTT'AAA Cuts at: 2800 3929 2800 Size: 1129 5496 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5632 809 Size: 507 4316 1802 Fragments arranged by size: 4316 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4072 4961 6590 Size: 54 1425 50 275 18 2242 889 1629 Cuts at: 6590 8 Size: 43 Fragments arranged by size: 2242 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4072 1537 Size: 275 2260 4090 Fragments arranged by size: 4090 2260 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3477 4555 1353 Size: 2124 1078 3423 Fragments arranged by size: 3423 2124 1078 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5212 5586 5732 387 Size: 1064 138 3623 374 146 1280 Fragments arranged by size: 3623 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2302 2346 2672 3192 3493 3549 3729 Size: 393 670 44 326 520 301 56 180 Cuts at: 3729 4688 5285 6072 6340 1239 Size: 959 597 787 268 1524 Fragments arranged by size: 1524 959 787 670 597 520 393 326 301 268 180 56 44 EcoNI CCTnn'n_nnAGG Cuts at: 2033 2033 Size: 6625 EcoO109I rG'GnC_Cy Cuts at: 2026 2767 2026 Size: 741 5884 EcoRI G'AATT_C Cuts at: 2758 3934 4312 2758 Size: 1176 378 5071 Fragments arranged by size: 5071 1176 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3661 3661 Size: 6625 FspI TGC'GCA Cuts at: 5131 5131 Size: 6625 HaeII r_GCGC'y Cuts at: 1678 2047 2129 3739 5772 1678 Size: 369 82 1610 2033 2531 Fragments arranged by size: 2531 2033 1610 369 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2160 2192 2420 2452 2705 Size: 32 135 32 588 32 228 32 253 Cuts at: 2705 2737 2831 2863 2930 2962 3905 3937 5266 Size: 32 94 32 67 32 943 32 1329 Cuts at: 5266 5298 5340 5372 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1329 943 588 253 228 135 94 67 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2553 3241 3969 4215 669 Size: 988 281 615 688 728 246 3079 Fragments arranged by size: 3079 988 728 688 615 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6625 HpaI GTT'AAC Cuts at: 4215 4215 Size: 6625 KpnI G_GTAC'C Cuts at: 3950 3950 Size: 6625 MfeI C'AATT_G Cuts at: 2341 3570 4224 2341 Size: 1229 654 4742 Fragments arranged by size: 4742 1229 654 MluI A'CGCG_T Cuts at: 6501 6501 Size: 6625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3216 5739 5923 1305 Size: 1911 2523 184 2007 Fragments arranged by size: 2523 2007 1911 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4803 Fragments arranged by size: 4803 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4583 4942 5101 518 Size: 648 3417 359 159 2042 Fragments arranged by size: 3417 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3097 3136 4041 4718 5866 6111 1606 Size: 1491 39 905 677 1148 245 2120 Fragments arranged by size: 2120 1491 1148 905 677 245 39 NcoI C'CATG_G Cuts at: 513 1065 2004 3555 513 Size: 552 939 1551 3583 Fragments arranged by size: 3583 1551 939 552 NdeI CA'TA_TG Cuts at: 387 2479 387 Size: 2092 4533 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6625 NotI GC'GGCC_GC Cuts at: 4072 4072 Size: 6625 NspI r_CATG'y Cuts at: 1132 1229 2099 3683 3983 5528 1132 Size: 97 870 1584 300 1545 2229 Fragments arranged by size: 2229 1584 1545 870 300 97 PasI CC'CwG_GG Cuts at: 1348 2725 1348 Size: 1377 5248 PciI A'CATG_T Cuts at: 5524 5524 Size: 6625 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6625 PmeI GTTT'AAAC Cuts at: 3929 3929 Size: 6625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4694 4726 6207 6239 4694 Size: 32 1481 32 5080 Fragments arranged by size: 5080 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2767 2767 Size: 6625 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6625 PsiI TTA'TAA Cuts at: 3636 4195 3636 Size: 559 6066 PstI C_TGCA'G Cuts at: 830 2899 3088 3977 830 Size: 2069 189 889 3478 Fragments arranged by size: 3478 2069 889 189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6625 SalI G'TCGA_C Cuts at: 1936 3967 1936 Size: 2031 4594 SbfI CC_TGCA'GG Cuts at: 3977 3977 Size: 6625 ScaI AGT'ACT Cuts at: 4873 6585 4873 Size: 1712 4913 SfcI C'TryA_G Cuts at: 826 1045 2895 3084 3696 3973 5108 5789 5980 Size: 219 1850 189 612 277 1135 681 191 Cuts at: 5980 826 Size: 1471 Fragments arranged by size: 1850 1471 1135 681 612 277 219 191 189 SfiI GGCCn_nnn'nGGCC Cuts at: 2654 2654 Size: 6625 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6625 SmaI CCC'GGG Cuts at: 1872 3952 1872 Size: 2080 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2129 2514 3225 3473 4729 5630 Size: 197 926 186 385 711 248 1256 901 Cuts at: 5630 5892 820 Size: 262 1553 Fragments arranged by size: 1553 1256 926 901 711 385 262 248 197 186 SnaBI TAC'GTA Cuts at: 493 4416 493 Size: 3923 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6625 SphI G_CATG'C Cuts at: 1132 3683 3983 1132 Size: 2551 300 3774 Fragments arranged by size: 3774 2551 300 SspI AAT'ATT Cuts at: 5 52 4549 5 Size: 47 4497 2081 Fragments arranged by size: 4497 2081 47 StuI AGG'CCT Cuts at: 1517 2657 3090 1517 Size: 1140 433 5052 Fragments arranged by size: 5052 1140 433 StyI C'CwwG_G Cuts at: 513 1065 2004 2185 3555 4064 513 Size: 552 939 181 1370 509 3074 Fragments arranged by size: 3074 1370 939 552 509 181 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4652 4669 4822 5007 5452 325 Size: 1255 3072 17 153 185 445 1498 Fragments arranged by size: 3072 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3666 4871 6583 Size: 274 80 33 51 1443 1689 1205 1712 Cuts at: 6583 96 Size: 138 Fragments arranged by size: 1712 1689 1443 1205 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3371 3403 6585 6617 3371 Size: 32 3182 32 3379 Fragments arranged by size: 3379 3182 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6625 XbaI T'CTAG_A Cuts at: 2867 3961 2867 Size: 1094 5531 XhoI C'TCGA_G Cuts at: 1943 2129 1943 Size: 186 6439 XmaI C'CCGG_G Cuts at: 1870 3950 1870 Size: 2080 4545 XmnI GAAnn'nnTTC Cuts at: 3852 4754 3852 Size: 902 5723 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4433 276 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI