(Circular) (Six-base) MAPSORT of: pFN21AB6603.seq Check: 8303 from: 1 to: 5929 pFN21AB6603 5929 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3739 278 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3272 1937 Size: 1335 4594 Acc65I G'GTAC_C Cuts at: 2143 3250 2143 Size: 1107 4822 AclI AA'CG_TT Cuts at: 1563 4056 4429 1563 Size: 2493 373 3063 Fragments arranged by size: 3063 2493 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2319 2969 3992 5376 5644 1632 Size: 687 650 1023 1384 268 1917 Fragments arranged by size: 1917 1384 1023 687 650 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5732 AflIII A'CryG_T Cuts at: 1203 4828 5805 1203 Size: 3625 977 1327 Fragments arranged by size: 3625 1327 977 AgeI A'CCGG_T Cuts at: 4760 4760 Size: 5929 AhdI GACnn_n'nnGTC Cuts at: 4658 4658 Size: 5929 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5929 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5929 AlwNI CAG_nnn'CTG Cuts at: 3672 5244 3672 Size: 1572 4357 ApaLI G'TGCA_C Cuts at: 3986 5142 3986 Size: 1156 4773 ApoI r'AATT_y Cuts at: 1493 2165 2501 2708 3092 3238 3464 3616 5725 Size: 672 336 207 384 146 226 152 2109 Cuts at: 5725 1493 Size: 1697 Fragments arranged by size: 2109 1697 672 384 336 226 207 152 146 AseI AT'TA_AT Cuts at: 160 1032 4483 160 Size: 872 3451 1606 Fragments arranged by size: 3451 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 AvaI C'yCGr_G Cuts at: 1870 1943 3254 1870 Size: 73 1311 4545 Fragments arranged by size: 4545 1311 73 BamHI G'GATC_C Cuts at: 3259 3626 3259 Size: 367 5562 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2143 3250 4705 618 Size: 325 217 627 356 1107 1455 1842 Fragments arranged by size: 1842 1455 1107 627 356 325 217 BanII G_rGCy'C Cuts at: 1391 3248 1391 Size: 1857 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2970 928 Size: 971 1071 3887 Fragments arranged by size: 3887 1071 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2552 2586 4120 4154 1903 Size: 34 615 34 1534 34 3678 Fragments arranged by size: 3678 1534 615 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3821 5031 3821 Size: 1210 4719 BclI T'GATC_A Cuts at: 1548 2728 1548 Size: 1180 4749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2045 4540 136 Size: 107 122 71 631 605 373 2495 1525 Fragments arranged by size: 2495 1525 631 605 373 122 107 71 BglII A'GATC_T Cuts at: 2336 5924 2336 Size: 3588 2341 BlpI GC'TnA_GC Cuts at: 3346 3346 Size: 5929 Bme1580I G_kGCm'C Cuts at: 1790 3990 5146 1790 Size: 2200 1156 2573 Fragments arranged by size: 2573 2200 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5929 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3660 4618 442 Size: 416 53 83 399 24 2243 958 1753 Fragments arranged by size: 2243 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5929 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 3240 4589 1239 Size: 824 1177 1349 2579 Fragments arranged by size: 2579 1349 1177 824 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4054 4919 5217 4054 Size: 865 298 4766 Fragments arranged by size: 4766 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2283 2504 3216 4592 882 Size: 617 784 221 712 1376 2219 Fragments arranged by size: 2219 1376 784 712 617 221 BsaAI yAC'GTr Cuts at: 493 1204 3720 493 Size: 711 2516 2702 Fragments arranged by size: 2702 2516 711 BsaBI GATnn'nnATC Cuts at: 5923 5923 Size: 5929 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3736 4118 4753 275 Size: 53 83 186 936 2203 382 635 1451 Fragments arranged by size: 2203 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4362 4760 5034 5181 5592 4362 Size: 398 274 147 411 4699 Fragments arranged by size: 4699 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3213 3243 1178 Size: 30 165 30 1810 30 3864 Fragments arranged by size: 3864 1810 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2817 1186 Size: 1631 4298 BseYI C'CCAG_C Cuts at: 2139 3042 5132 5628 2139 Size: 903 2090 496 2440 Fragments arranged by size: 2440 2090 903 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5356 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3379 4140 5168 1540 Size: 275 186 1378 761 1028 2301 Fragments arranged by size: 2301 1378 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3990 4075 5146 3990 Size: 85 1071 4773 Fragments arranged by size: 4773 1071 85 BsmI GAATG_Cn' Cuts at: 3440 3533 3440 Size: 93 5836 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3248 3990 4075 5146 1391 Size: 399 1458 742 85 1071 2174 Fragments arranged by size: 2174 1458 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5592 5592 Size: 5929 BspHI T'CATG_A Cuts at: 3818 5548 3818 Size: 1730 4199 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2550 3193 3284 844 Size: 1706 643 91 3489 Fragments arranged by size: 3489 1706 643 91 BsrBI CCG'CTC Cuts at: 1394 1823 3816 1394 Size: 429 1993 3507 Fragments arranged by size: 3507 1993 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2438 4424 4598 65 Size: 1183 1190 1986 174 1396 Fragments arranged by size: 1986 1396 1190 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4573 4760 1700 Size: 110 2763 187 2869 Fragments arranged by size: 2869 2763 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5929 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5929 BssSI C'ACGA_G Cuts at: 3989 5001 3989 Size: 1012 4917 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5929 BstBI TT'CG_AA Cuts at: 4733 4733 Size: 5929 BstEII G'GTnAC_C Cuts at: 1184 2879 1184 Size: 1695 4234 BstYI r'GATC_y Cuts at: 1972 2336 3259 3626 4011 4028 5480 5566 5578 Size: 364 923 367 385 17 1452 86 12 Cuts at: 5578 5924 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 923 385 367 364 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2096 513 Size: 552 939 92 4346 Fragments arranged by size: 4346 939 552 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5190 Fragments arranged by size: 5190 683 56 BtsI GCAGTG_nn' Cuts at: 3445 4239 4259 3445 Size: 794 20 5115 Fragments arranged by size: 5115 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5929 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5894 DraI TTT'AAA Cuts at: 2830 3013 3233 2830 Size: 183 220 5526 Fragments arranged by size: 5526 220 183 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4936 809 Size: 507 3620 1802 Fragments arranged by size: 3620 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2518 3376 4265 Size: 54 1425 50 275 18 688 858 889 Cuts at: 4265 5894 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 858 688 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3376 1537 Size: 275 1564 4090 Fragments arranged by size: 4090 1564 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3859 1353 Size: 2506 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4516 4890 5036 387 Size: 1064 138 2927 374 146 1280 Fragments arranged by size: 2927 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2319 2969 3240 3992 4589 5376 Size: 393 431 256 650 271 752 597 787 Cuts at: 5376 5644 1239 Size: 268 1524 Fragments arranged by size: 1524 787 752 650 597 431 393 271 268 256 EcoRI G'AATT_C Cuts at: 3238 3616 3238 Size: 378 5551 FspI TGC'GCA Cuts at: 4435 4435 Size: 5929 HaeII r_GCGC'y Cuts at: 1678 5076 1678 Size: 3398 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4570 4602 4644 4676 1373 Size: 32 135 32 2998 32 42 32 2626 Fragments arranged by size: 2998 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3273 3519 669 Size: 988 281 1335 246 3079 Fragments arranged by size: 3079 1335 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5929 HpaI GTT'AAC Cuts at: 3519 3519 Size: 5929 KpnI G_GTAC'C Cuts at: 2147 3254 2147 Size: 1107 4822 MfeI C'AATT_G Cuts at: 3528 3528 Size: 5929 MluI A'CGCG_T Cuts at: 5805 5805 Size: 5929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5043 5227 1305 Size: 3738 184 2007 Fragments arranged by size: 3738 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2520 10 Size: 54 1425 343 688 3419 Fragments arranged by size: 3419 1425 688 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3887 4246 4405 518 Size: 648 2721 359 159 2042 Fragments arranged by size: 2721 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3345 4022 5170 5415 1606 Size: 1739 677 1148 245 2120 Fragments arranged by size: 2120 1739 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4438 Fragments arranged by size: 4438 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5929 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5929 NotI GC'GGCC_GC Cuts at: 3376 3376 Size: 5929 NspI r_CATG'y Cuts at: 1132 1229 3287 4832 1132 Size: 97 2058 1545 2229 Fragments arranged by size: 2229 2058 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5929 PciI A'CATG_T Cuts at: 4828 4828 Size: 5929 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5929 PmeI GTTT'AAAC Cuts at: 3233 3233 Size: 5929 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3998 4030 5511 5543 3998 Size: 32 1481 32 4384 Fragments arranged by size: 4384 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5929 PsiI TTA'TAA Cuts at: 3499 3499 Size: 5929 PstI C_TGCA'G Cuts at: 830 3281 830 Size: 2451 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5929 SalI G'TCGA_C Cuts at: 1936 3271 1936 Size: 1335 4594 SbfI CC_TGCA'GG Cuts at: 3281 3281 Size: 5929 ScaI AGT'ACT Cuts at: 2415 2919 4177 5889 2415 Size: 504 1258 1712 2455 Fragments arranged by size: 2455 1712 1258 504 SexAI A'CCwGG_T Cuts at: 3225 3225 Size: 5929 SfcI C'TryA_G Cuts at: 826 1045 2737 3277 4412 5093 5284 826 Size: 219 1692 540 1135 681 191 1471 Fragments arranged by size: 1692 1471 1135 681 540 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5929 SmaI CCC'GGG Cuts at: 1872 3256 1872 Size: 1384 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4033 4934 5196 820 Size: 197 926 2090 901 262 1553 Fragments arranged by size: 2090 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3720 493 Size: 3227 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5929 SphI G_CATG'C Cuts at: 1132 3287 1132 Size: 2155 3774 SspI AAT'ATT Cuts at: 5 52 2422 3853 5 Size: 47 2370 1431 2081 Fragments arranged by size: 2370 2081 1431 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5929 StyI C'CwwG_G Cuts at: 513 1065 2004 2229 2841 3368 513 Size: 552 939 225 612 527 3074 Fragments arranged by size: 3074 939 612 552 527 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3956 3973 4126 4311 4756 325 Size: 1255 2376 17 153 185 445 1498 Fragments arranged by size: 2376 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2413 2526 2917 Size: 274 80 33 51 1443 436 113 391 Cuts at: 2917 3067 4175 5887 96 Size: 150 1108 1712 138 Fragments arranged by size: 1712 1443 1108 436 391 274 150 138 113 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5889 5921 5889 Size: 32 5897 Tth111I GACn'n_nGTC Cuts at: 1316 2795 1316 Size: 1479 4450 XbaI T'CTAG_A Cuts at: 2680 3265 2680 Size: 585 5344 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2848 3050 2848 Size: 202 5727 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5929 XmaI C'CCGG_G Cuts at: 1870 3254 1870 Size: 1384 4545 XmnI GAAnn'nnTTC Cuts at: 4058 4058 Size: 5929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3737 276 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI