(Circular) (Six-base) MAPSORT of: pFN21AA0708.seq Check: 4528 from: 1 to: 12256 pFN21AA0708 12256 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 8354 8552 8354 Size: 198 12058 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 10066 278 Size: 53 83 186 936 8530 2468 Fragments arranged by size: 8530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2220 3269 3752 8634 9599 1937 Size: 283 1049 483 4882 965 4594 Fragments arranged by size: 4882 4594 1049 965 483 283 Acc65I G'GTAC_C Cuts at: 9577 9577 Size: 12256 AclI AA'CG_TT Cuts at: 1563 10383 10756 1563 Size: 8820 373 3063 Fragments arranged by size: 8820 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2091 2159 2255 2473 2733 3330 4418 6328 Size: 459 68 96 218 260 597 1088 1910 Cuts at: 6328 6710 7406 9290 10319 11703 11971 1632 Size: 382 696 1884 1029 1384 268 1917 Fragments arranged by size: 1917 1910 1884 1384 1088 1029 696 597 459 382 268 260 218 96 68 AfeI AGC'GCT Cuts at: 2789 3101 5843 8708 2789 Size: 312 2742 2865 6337 Fragments arranged by size: 6337 2865 2742 312 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 12059 AflIII A'CryG_T Cuts at: 1203 2523 4689 5945 11155 12132 1203 Size: 1320 2166 1256 5210 977 1327 Fragments arranged by size: 5210 2166 1327 1320 1256 977 AgeI A'CCGG_T Cuts at: 11087 11087 Size: 12256 AhdI GACnn_n'nnGTC Cuts at: 8034 10985 8034 Size: 2951 9305 AleI CACnn'nnGTG Cuts at: 1166 5637 6639 7491 1166 Size: 4471 1002 852 5931 Fragments arranged by size: 5931 4471 1002 852 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2588 2762 6078 9150 9251 9297 1184 Size: 1404 174 3316 3072 101 46 4143 Fragments arranged by size: 4143 3316 3072 1404 174 101 46 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2891 2923 2891 Size: 32 12224 AlwNI CAG_nnn'CTG Cuts at: 2368 2581 3353 3940 4258 5173 5290 6103 6913 Size: 213 772 587 318 915 117 813 810 Cuts at: 6913 7309 7849 7949 8119 8527 9019 9999 11571 Size: 396 540 100 170 408 492 980 1572 Cuts at: 11571 2368 Size: 3053 Fragments arranged by size: 3053 1572 980 915 813 810 772 587 540 492 408 396 318 213 170 117 100 ApaI G_GGCC'C Cuts at: 2475 2965 3320 4880 5025 5897 6487 7521 7881 Size: 490 355 1560 145 872 590 1034 360 Cuts at: 7881 2475 Size: 6850 Fragments arranged by size: 6850 1560 1034 872 590 490 360 355 145 ApaLI G'TGCA_C Cuts at: 3290 8482 8902 10313 11469 3290 Size: 5192 420 1411 1156 4077 Fragments arranged by size: 5192 4077 1411 1156 420 ApoI r'AATT_y Cuts at: 1493 3107 6058 7172 7346 9565 9791 9943 12052 Size: 1614 2951 1114 174 2219 226 152 2109 Cuts at: 12052 1493 Size: 1697 Fragments arranged by size: 2951 2219 2109 1697 1614 1114 226 174 152 AseI AT'TA_AT Cuts at: 160 1032 10810 160 Size: 872 9778 1606 Fragments arranged by size: 9778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12256 AvaI C'yCGr_G Cuts at: 1870 1943 2307 4525 5868 6264 6558 9425 9581 Size: 73 364 2218 1343 396 294 2867 156 Cuts at: 9581 1870 Size: 4545 Fragments arranged by size: 4545 2867 2218 1343 396 364 294 156 73 AvrII C'CTAG_G Cuts at: 3604 3604 Size: 12256 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3757 3790 3757 Size: 33 12223 BamHI G'GATC_C Cuts at: 9246 9586 9953 9246 Size: 340 367 11549 Fragments arranged by size: 11549 367 340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 4940 6006 6379 6599 Size: 325 217 627 251 2902 1066 373 220 Cuts at: 6599 6732 8748 9577 11032 618 Size: 133 2016 829 1455 1842 Fragments arranged by size: 2902 2016 1842 1455 1066 829 627 373 325 251 220 217 133 BanII G_rGCy'C Cuts at: 1391 2475 2506 2611 2965 3320 3879 4110 4532 Size: 1084 31 105 354 355 559 231 422 Cuts at: 4532 4880 5025 5121 5483 5751 5897 6377 6487 Size: 348 145 96 362 268 146 480 110 Cuts at: 6487 6700 7521 7872 7881 8000 8231 9575 1391 Size: 213 821 351 9 119 231 1344 4072 Fragments arranged by size: 4072 1344 1084 821 559 480 422 362 355 354 351 348 268 231 231 213 146 145 119 110 105 96 31 9 BbeI G_GCGC'C Cuts at: 6010 6383 6603 6010 Size: 373 220 11663 Fragments arranged by size: 11663 373 220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2479 3082 5415 7619 9296 9411 928 Size: 971 580 603 2333 2204 1677 115 3773 Fragments arranged by size: 3773 2333 2204 1677 971 603 580 115 BbvCI CC'TCA_GC Cuts at: 2972 5051 9218 2972 Size: 2079 4167 6010 Fragments arranged by size: 6010 4167 2079 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5918 5952 10447 10481 1903 Size: 34 3981 34 4495 34 3678 Fragments arranged by size: 4495 3981 3678 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 10148 11358 10148 Size: 1210 11046 BclI T'GATC_A Cuts at: 1548 7675 1548 Size: 6127 6129 BfrBI ATG'CAT Cuts at: 5206 5206 Size: 12256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2047 3123 4926 Size: 107 122 71 631 605 375 1076 1803 Cuts at: 4926 5139 5853 6098 7876 8523 9332 9430 10867 Size: 213 714 245 1778 647 809 98 1437 Cuts at: 10867 136 Size: 1525 Fragments arranged by size: 1803 1778 1525 1437 1076 809 714 647 631 605 375 245 213 122 107 98 71 BglII A'GATC_T Cuts at: 3612 3705 4494 12251 3612 Size: 93 789 7757 3617 Fragments arranged by size: 7757 3617 789 93 BlpI GC'TnA_GC Cuts at: 5283 6434 7284 8076 9673 5283 Size: 1151 850 792 1597 7866 Fragments arranged by size: 7866 1597 1151 850 792 Bme1580I G_kGCm'C Cuts at: 1790 2043 2257 2475 2965 3294 3320 3389 4880 Size: 253 214 218 490 329 26 69 1491 Cuts at: 4880 5025 5897 5971 6184 6487 7434 7521 7881 Size: 145 872 74 213 303 947 87 360 Cuts at: 7881 8486 8906 8976 9426 10317 11473 1790 Size: 605 420 70 450 891 1156 2573 Fragments arranged by size: 2573 1491 1156 947 891 872 605 490 450 420 360 329 303 253 218 214 213 145 87 74 70 69 26 BmgBI CAC'GTC Cuts at: 1318 2614 2805 7269 1318 Size: 1296 191 4464 6305 Fragments arranged by size: 6305 4464 1296 191 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3295 5593 6113 Size: 416 53 83 399 24 1878 2298 520 Cuts at: 6113 6418 8110 9987 10945 442 Size: 305 1692 1877 958 1753 Fragments arranged by size: 2298 1878 1877 1753 1692 958 520 416 399 305 83 53 24 BmtI G_CTAG'C Cuts at: 1056 8610 1056 Size: 7554 4702 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2625 3936 4609 5272 5548 8437 8482 2625 Size: 1311 673 663 276 2889 45 6399 Fragments arranged by size: 6399 2889 1311 673 663 276 45 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2705 2868 2924 3097 3137 3326 3431 4952 Size: 1466 163 56 173 40 189 105 1521 Cuts at: 4952 5347 5467 5998 5999 6723 6863 6944 7183 Size: 395 120 531 1 724 140 81 239 Cuts at: 7183 7403 7718 7848 9041 9140 9173 10916 1239 Size: 220 315 130 1193 99 33 1743 2579 Fragments arranged by size: 2579 1743 1521 1466 1193 724 531 395 315 239 220 189 173 163 140 130 120 105 99 81 56 40 33 1 Bpu10I CC'TnA_GC Cuts at: 2538 2972 4797 4848 5051 5898 6324 6821 6914 Size: 434 1825 51 203 847 426 497 93 Cuts at: 6914 8142 8184 9218 2538 Size: 1228 42 1034 5576 Fragments arranged by size: 5576 1825 1228 1034 847 497 434 426 203 93 51 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4943 5668 6259 7639 8786 10381 11246 11544 4943 Size: 725 591 1380 1147 1595 865 298 5655 Fragments arranged by size: 5655 1595 1380 1147 865 725 591 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2844 3578 3890 4653 4808 4970 6215 Size: 617 1345 734 312 763 155 162 1245 Cuts at: 6215 7370 9171 10919 882 Size: 1155 1801 1748 2219 Fragments arranged by size: 2219 1801 1748 1345 1245 1155 763 734 617 312 162 155 BsaAI yAC'GTr Cuts at: 493 1204 2446 7819 10047 493 Size: 711 1242 5373 2228 2702 Fragments arranged by size: 5373 2702 2228 1242 711 BsaBI GATnn'nnATC Cuts at: 12250 12250 Size: 12256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6007 6380 6600 10063 Size: 53 83 186 936 4474 373 220 3463 Cuts at: 10063 10445 11080 275 Size: 382 635 1451 Fragments arranged by size: 4474 3463 1451 936 635 382 373 220 186 83 53 BsaWI w'CCGG_w Cuts at: 6369 8378 10689 11087 11361 11508 11919 6369 Size: 2009 2311 398 274 147 411 6706 Fragments arranged by size: 6706 2311 2009 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2843 2873 3123 3153 3785 Size: 30 165 30 1440 30 250 30 632 Cuts at: 3785 3815 4929 4959 5345 5375 5492 5522 5918 Size: 30 1114 30 386 30 117 30 396 Cuts at: 5918 5948 7181 7211 7526 7556 8864 8894 9170 Size: 30 1233 30 315 30 1308 30 276 Cuts at: 9170 9200 9458 9488 1178 Size: 30 258 30 3946 Fragments arranged by size: 3946 1440 1308 1233 1114 632 396 386 315 276 258 250 165 117 30 30 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3206 3806 4148 4411 4535 4946 6002 6297 Size: 2020 600 342 263 124 411 1056 295 Cuts at: 6297 6941 7013 7037 7040 7049 7376 8395 9178 Size: 644 72 24 3 9 327 1019 783 Cuts at: 9178 9364 9410 9413 9467 1186 Size: 186 46 3 54 3975 Fragments arranged by size: 3975 2020 1056 1019 783 644 600 411 342 327 295 263 186 124 72 54 46 24 9 3 3 BseYI C'CCAG_C Cuts at: 2024 2285 2597 2839 2958 3038 3203 4382 4980 Size: 261 312 242 119 80 165 1179 598 Cuts at: 4980 5293 5395 5511 6117 6715 7273 7521 7751 Size: 313 102 116 606 598 558 248 230 Cuts at: 7751 7937 8162 8370 8559 8714 11459 11955 2024 Size: 186 225 208 189 155 2745 496 2325 Fragments arranged by size: 2745 2325 1179 606 598 598 558 496 313 312 261 248 242 230 225 208 189 186 165 155 119 116 102 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2580 3161 3272 4088 4271 4394 4824 Size: 573 844 581 111 816 183 123 430 Cuts at: 4824 5609 6164 6464 7346 7781 8063 8738 9110 Size: 785 555 300 882 435 282 675 372 Cuts at: 9110 9193 1163 Size: 83 4226 Fragments arranged by size: 4226 882 844 816 785 675 581 573 555 435 430 372 300 282 183 123 111 83 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2424 4056 4301 8506 9706 10467 Size: 275 186 423 1632 245 4205 1200 761 Cuts at: 10467 11495 1540 Size: 1028 2301 Fragments arranged by size: 4205 2301 1632 1200 1028 761 423 275 245 186 BsiHKAI G_wGCw'C Cuts at: 2190 2451 3294 4595 5229 5751 6525 6789 6920 Size: 261 843 1301 634 522 774 264 131 Cuts at: 6920 8486 8906 10317 10402 11473 2190 Size: 1566 420 1411 85 1071 2973 Fragments arranged by size: 2973 1566 1411 1301 1071 843 774 634 522 420 264 261 131 85 BsiWI C'GTAC_G Cuts at: 8197 8197 Size: 12256 BsmI GAATG_Cn' Cuts at: 2739 3240 5382 9277 9767 9860 2739 Size: 501 2142 3895 490 93 5135 Fragments arranged by size: 5135 3895 2142 501 490 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6821 7129 8042 8407 6821 Size: 308 913 365 10670 Fragments arranged by size: 10670 913 365 308 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2190 2257 2451 2475 2506 2611 Size: 399 253 147 67 194 24 31 105 Cuts at: 2611 2965 3294 3320 3389 3879 4110 4532 4595 Size: 354 329 26 69 490 231 422 63 Cuts at: 4595 4880 5025 5121 5229 5483 5751 5897 5971 Size: 285 145 96 108 254 268 146 74 Cuts at: 5971 6184 6377 6487 6525 6700 6789 6920 7434 Size: 213 193 110 38 175 89 131 514 Cuts at: 7434 7521 7872 7881 8000 8231 8486 8906 8976 Size: 87 351 9 119 231 255 420 70 Cuts at: 8976 9426 9575 10317 10402 11473 1391 Size: 450 149 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 514 490 450 422 420 399 354 351 329 285 268 255 254 253 231 231 213 194 193 175 149 147 146 145 131 119 110 108 105 96 89 87 85 74 70 69 67 63 38 31 26 24 9 BspEI T'CCGG_A Cuts at: 11919 11919 Size: 12256 BspHI T'CATG_A Cuts at: 4278 4755 7507 10145 11875 4278 Size: 477 2752 2638 1730 4659 Fragments arranged by size: 4659 2752 2638 1730 477 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2520 3244 3640 3699 4101 4281 5186 6122 Size: 1676 724 396 59 402 180 905 936 Cuts at: 6122 6442 8259 8354 8552 9611 844 Size: 320 1817 95 198 1059 3489 Fragments arranged by size: 3489 1817 1676 1059 936 905 724 402 396 320 198 180 95 59 BsrBI CCG'CTC Cuts at: 1394 1823 2251 4147 4303 7862 10143 1394 Size: 429 428 1896 156 3559 2281 3507 Fragments arranged by size: 3559 3507 2281 1896 429 428 156 BsrDI GCAATG_nn' Cuts at: 65 1248 2343 2678 5094 8352 10751 10925 65 Size: 1183 1095 335 2416 3258 2399 174 1396 Fragments arranged by size: 3258 2416 2399 1396 1183 1095 335 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5375 5603 6003 7836 10900 11087 1700 Size: 110 3565 228 400 1833 3064 187 2869 Fragments arranged by size: 3565 3064 2869 1833 400 228 187 110 BsrGI T'GTAC_A Cuts at: 96 9057 96 Size: 8961 3295 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12256 BssSI C'ACGA_G Cuts at: 4289 5105 6050 7427 10316 11328 4289 Size: 816 945 1377 2889 1012 5217 Fragments arranged by size: 5217 2889 1377 1012 945 816 BstAPI GCAn_nnn'nTGC Cuts at: 794 4258 5733 6114 7309 8032 8514 794 Size: 3464 1475 381 1195 723 482 4536 Fragments arranged by size: 4536 3464 1475 1195 723 482 381 BstBI TT'CG_AA Cuts at: 11060 11060 Size: 12256 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 12256 BstXI CCAn_nnnn'nTGG Cuts at: 2392 2597 2639 3994 4228 5266 5371 5707 7116 Size: 205 42 1355 234 1038 105 336 1409 Cuts at: 7116 7529 2392 Size: 413 7119 Fragments arranged by size: 7119 1409 1355 1038 413 336 234 205 105 42 BstYI r'GATC_y Cuts at: 1972 2311 2547 2754 3309 3612 3705 3781 4428 Size: 339 236 207 555 303 93 76 647 Cuts at: 4428 4494 5250 5829 6672 7072 7845 8984 9144 Size: 66 756 579 843 400 773 1139 160 Cuts at: 9144 9246 9586 9953 10338 10355 11807 11893 11905 Size: 102 340 367 385 17 1452 86 12 Cuts at: 11905 12251 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1139 843 773 756 647 579 555 400 385 367 346 340 339 303 236 207 160 102 93 86 76 66 17 12 Bsu36I CC'TnA_GG Cuts at: 2044 2214 3321 5082 5462 6410 7441 8589 9376 Size: 170 1107 1761 380 948 1031 1148 787 Cuts at: 9376 2044 Size: 4924 Fragments arranged by size: 4924 1761 1148 1107 1031 948 787 380 170 BtgI C'CryG_G Cuts at: 513 1065 2004 3624 3903 5635 7651 7872 8779 Size: 552 939 1620 279 1732 2016 221 907 Cuts at: 8779 8847 513 Size: 68 3922 Fragments arranged by size: 3922 2016 1732 1620 939 907 552 279 221 68 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5174 487 Size: 683 56 3948 7569 Fragments arranged by size: 7569 3948 683 56 BtsI GCAGTG_nn' Cuts at: 2719 5155 6592 6624 7268 7534 8147 8209 9772 Size: 2436 1437 32 644 266 613 62 1563 Cuts at: 9772 10566 10586 2719 Size: 794 20 4389 Fragments arranged by size: 4389 2436 1563 1437 794 644 613 266 62 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 12256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6763 6798 529 Size: 35 6199 35 5987 Fragments arranged by size: 6199 5987 35 35 DraI TTT'AAA Cuts at: 9560 9560 Size: 12256 DraIII CAC_nnn'GTG Cuts at: 3914 3914 Size: 12256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 11263 809 Size: 507 9947 1802 Fragments arranged by size: 9947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2382 2421 2999 Size: 54 1425 50 275 18 552 39 578 Cuts at: 2999 3633 3996 4059 4298 4407 4447 5373 5670 Size: 634 363 63 239 109 40 926 297 Cuts at: 5670 5912 6771 8614 8688 9703 10592 12221 8 Size: 242 859 1843 74 1015 889 1629 43 Fragments arranged by size: 1843 1629 1425 1015 926 889 859 634 578 552 363 297 275 242 239 109 74 63 54 50 43 40 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2421 4298 9703 1537 Size: 275 609 1877 5405 4090 Fragments arranged by size: 5405 4090 1877 609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3783 3819 4600 5957 6956 7014 7026 7035 Size: 2430 36 781 1357 999 58 12 9 Cuts at: 7035 7601 9387 9525 10186 1353 Size: 566 1786 138 661 3423 Fragments arranged by size: 3423 2430 1786 1357 999 781 661 566 138 58 36 12 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2540 3083 6824 9259 10843 11217 Size: 1064 138 951 543 3741 2435 1584 374 Cuts at: 11217 11363 387 Size: 146 1280 Fragments arranged by size: 3741 2435 1584 1280 1064 951 543 374 146 138 EcoICRI GAG'CTC Cuts at: 5749 5749 Size: 12256 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2091 2159 2255 2473 2705 2733 2868 Size: 393 459 68 96 218 232 28 135 Cuts at: 2868 2924 3097 3137 3326 3330 3431 4418 4952 Size: 56 173 40 189 4 101 987 534 Cuts at: 4952 5347 5467 5998 5999 6328 6710 6723 6863 Size: 395 120 531 1 329 382 13 140 Cuts at: 6863 6944 7183 7403 7406 7718 7848 9041 9140 Size: 81 239 220 3 312 130 1193 99 Cuts at: 9140 9173 9290 10319 10916 11703 11971 1239 Size: 33 117 1029 597 787 268 1524 Fragments arranged by size: 1524 1193 1029 987 787 597 534 531 459 395 393 382 329 312 268 239 232 220 218 189 173 140 135 130 120 117 101 99 96 81 68 56 40 33 28 13 4 3 1 EcoNI CCTnn'n_nnAGG Cuts at: 4121 4970 7466 9152 4121 Size: 849 2496 1686 7225 Fragments arranged by size: 7225 2496 1686 849 EcoO109I rG'GnC_Cy Cuts at: 2029 2048 2406 2472 3035 3317 3601 3856 4013 Size: 19 358 66 563 282 284 255 157 Cuts at: 4013 4255 4842 4876 4950 5021 5311 5887 5894 Size: 242 587 34 74 71 290 576 7 Cuts at: 5894 6124 6483 6484 7518 7537 7663 7877 7878 Size: 230 359 1 1034 19 126 214 1 Cuts at: 7878 8137 8524 9341 2029 Size: 259 387 817 4944 Fragments arranged by size: 4944 1034 817 587 576 563 387 359 358 290 284 282 259 255 242 230 214 157 126 74 71 66 34 19 19 7 1 1 EcoRI G'AATT_C Cuts at: 3107 7172 9565 9943 3107 Size: 4065 2393 378 5420 Fragments arranged by size: 5420 4065 2393 378 EcoRV GAT'ATC Cuts at: 3482 4074 3482 Size: 592 11664 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3550 6997 3550 Size: 3447 8809 FspI TGC'GCA Cuts at: 6584 10762 6584 Size: 4178 8078 HaeII r_GCGC'y Cuts at: 1678 2369 2791 3103 4966 5845 6010 6383 6603 Size: 691 422 312 1863 879 165 373 220 Cuts at: 6603 6940 8109 8441 8710 8797 11403 1678 Size: 337 1169 332 269 87 2606 2531 Fragments arranged by size: 2606 2531 1863 1169 879 691 422 373 337 332 312 269 220 165 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2118 2150 2843 2875 3785 Size: 32 135 32 546 32 693 32 910 Cuts at: 3785 3817 3818 3850 4443 4475 5261 5293 7181 Size: 32 1 32 593 32 786 32 1888 Cuts at: 7181 7213 7352 7384 7769 7801 8020 8052 8240 Size: 32 139 32 385 32 219 32 188 Cuts at: 8240 8272 9170 9202 9458 9490 10897 10929 10971 Size: 32 898 32 256 32 1407 32 42 Cuts at: 10971 11003 1373 Size: 32 2626 Fragments arranged by size: 2626 1888 1407 910 898 786 693 593 546 385 256 219 188 139 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 3270 8635 9600 9846 669 Size: 988 281 1332 5365 965 246 3079 Fragments arranged by size: 5365 3079 1332 988 965 281 246 HindIII A'AGCT_T Cuts at: 748 2299 748 Size: 1551 10705 HpaI GTT'AAC Cuts at: 9846 9846 Size: 12256 KasI G'GCGC_C Cuts at: 6006 6379 6599 6006 Size: 373 220 11663 Fragments arranged by size: 11663 373 220 KpnI G_GTAC'C Cuts at: 9581 9581 Size: 12256 MfeI C'AATT_G Cuts at: 9855 9855 Size: 12256 MluI A'CGCG_T Cuts at: 12132 12132 Size: 12256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3510 3783 5559 5616 8493 11370 11554 1305 Size: 2205 273 1776 57 2877 2877 184 2007 Fragments arranged by size: 2877 2877 2205 2007 1776 273 184 57 MscI TGG'CCA Cuts at: 10 64 1489 1832 3998 4061 5672 5914 6773 Size: 54 1425 343 2166 63 1611 242 859 Cuts at: 6773 8616 8690 10 Size: 1843 74 3576 Fragments arranged by size: 3576 2166 1843 1611 1425 859 343 242 74 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 2195 2444 2522 2918 3971 4754 5387 Size: 648 1029 249 78 396 1053 783 633 Cuts at: 5387 5637 6639 7491 8663 9092 9132 9227 10214 Size: 250 1002 852 1172 429 40 95 987 Cuts at: 10214 10573 10732 518 Size: 359 159 2042 Fragments arranged by size: 2042 1172 1053 1029 1002 987 852 783 648 633 429 396 359 250 249 159 95 78 40 MspA1I CmG'CkG Cuts at: 1606 2285 2392 2426 2821 2896 3353 3925 4052 Size: 679 107 34 395 75 457 572 127 Cuts at: 4052 5287 5733 5932 6730 6898 6964 7681 7975 Size: 1235 446 199 798 168 66 717 294 Cuts at: 7975 8116 8162 8718 9672 10349 11497 11742 1606 Size: 141 46 556 954 677 1148 245 2120 Fragments arranged by size: 2120 1235 1148 954 798 717 679 677 572 556 457 446 395 294 245 199 168 141 127 107 75 66 46 34 NaeI GCC'GGC Cuts at: 5377 5377 Size: 12256 NarI GG'CG_CC Cuts at: 6007 6380 6600 6007 Size: 373 220 11663 Fragments arranged by size: 11663 373 220 NcoI C'CATG_G Cuts at: 513 1065 2004 3903 7651 7872 8779 8847 513 Size: 552 939 1899 3748 221 907 68 3922 Fragments arranged by size: 3922 3748 1899 939 907 552 221 68 NdeI CA'TA_TG Cuts at: 387 8619 387 Size: 8232 4024 NgoMIV G'CCGG_C Cuts at: 5375 5375 Size: 12256 NheI G'CTAG_C Cuts at: 1052 8606 1052 Size: 7554 4702 NotI GC'GGCC_GC Cuts at: 4298 9703 4298 Size: 5405 6851 NsiI A_TGCA'T Cuts at: 5208 5208 Size: 12256 NspI r_CATG'y Cuts at: 1132 1229 2024 2527 2989 3304 4046 4348 5632 Size: 97 795 503 462 315 742 302 1284 Cuts at: 5632 5695 5949 6299 7316 8272 9352 9614 11159 Size: 63 254 350 1017 956 1080 262 1545 Cuts at: 11159 1132 Size: 2229 Fragments arranged by size: 2229 1545 1284 1080 1017 956 795 742 503 462 350 315 302 262 254 97 63 PasI CC'CwG_GG Cuts at: 1348 2234 3589 6184 7735 8233 8494 1348 Size: 886 1355 2595 1551 498 261 5110 Fragments arranged by size: 5110 2595 1551 1355 886 498 261 PciI A'CATG_T Cuts at: 2523 5945 11155 2523 Size: 3422 5210 3624 Fragments arranged by size: 5210 3624 3422 PflMI CCAn_nnn'nTGG Cuts at: 1108 3940 4159 4654 5910 6103 6289 8527 9019 Size: 2832 219 495 1256 193 186 2238 492 Cuts at: 9019 1108 Size: 4345 Fragments arranged by size: 4345 2832 2238 1256 495 492 219 193 186 PfoI T'CCnGG_A Cuts at: 2314 2811 4896 5265 5922 7233 7726 8807 9150 Size: 497 2085 369 657 1311 493 1081 343 Cuts at: 9150 9249 2314 Size: 99 5321 Fragments arranged by size: 5321 2085 1311 1081 657 497 493 369 343 99 PmeI GTTT'AAAC Cuts at: 9560 9560 Size: 12256 PmlI CAC'GTG Cuts at: 2446 2446 Size: 12256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3203 3235 6140 6172 6814 6846 10325 10357 11838 Size: 32 2905 32 642 32 3479 32 1481 Cuts at: 11838 11870 3203 Size: 32 3589 Fragments arranged by size: 3589 3479 2905 1481 642 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2029 2406 4013 4842 5311 5887 8137 9341 2029 Size: 377 1607 829 469 576 2250 1204 4944 Fragments arranged by size: 4944 2250 1607 1204 829 576 469 377 PshAI GACnn'nnGTC Cuts at: 1613 4364 1613 Size: 2751 9505 PsiI TTA'TAA Cuts at: 4670 9826 4670 Size: 5156 7100 PspOMI G'GGCC_C Cuts at: 2471 2961 3316 4876 5021 5893 6483 7517 7877 Size: 490 355 1560 145 872 590 1034 360 Cuts at: 7877 2471 Size: 6850 Fragments arranged by size: 6850 1560 1034 872 590 490 360 355 145 PstI C_TGCA'G Cuts at: 830 2061 2577 3651 4098 4561 4603 4888 6475 Size: 1231 516 1074 447 463 42 285 1587 Cuts at: 6475 6612 6909 6936 7263 7293 7984 8124 9144 Size: 137 297 27 327 30 691 140 1020 Cuts at: 9144 9276 9608 830 Size: 132 332 3478 Fragments arranged by size: 3478 1587 1231 1074 1020 691 516 463 447 332 327 297 285 140 137 132 42 30 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12256 PvuII CAG'CTG Cuts at: 2285 2392 2821 3353 3925 5287 5733 5932 6898 Size: 107 429 532 572 1362 446 199 966 Cuts at: 6898 7681 7975 8116 8162 8718 2285 Size: 783 294 141 46 556 5823 Fragments arranged by size: 5823 1362 966 783 572 556 532 446 429 294 199 141 107 46 RsrII CG'GwC_CG Cuts at: 6811 6811 Size: 12256 SacI G_AGCT'C Cuts at: 5751 5751 Size: 12256 SalI G'TCGA_C Cuts at: 1936 3268 8633 9598 1936 Size: 1332 5365 965 4594 Fragments arranged by size: 5365 4594 1332 965 SanDI GG'GwC_CC Cuts at: 2406 4013 4842 2406 Size: 1607 829 9820 Fragments arranged by size: 9820 1607 829 SapI GCTCTTCn'nnn_ Cuts at: 4600 4600 Size: 12256 SbfI CC_TGCA'GG Cuts at: 7984 9608 7984 Size: 1624 10632 ScaI AGT'ACT Cuts at: 6206 10504 12216 6206 Size: 4298 1712 6246 Fragments arranged by size: 6246 4298 1712 SexAI A'CCwGG_T Cuts at: 4224 5313 8477 4224 Size: 1089 3164 8003 Fragments arranged by size: 8003 3164 1089 SfcI C'TryA_G Cuts at: 826 1045 2057 2573 3647 4094 4557 4599 4884 Size: 219 1012 516 1074 447 463 42 285 Cuts at: 4884 5347 6471 6608 6905 6932 7207 7259 7289 Size: 463 1124 137 297 27 275 52 30 Cuts at: 7289 7970 7980 8120 8128 8578 9067 9140 9272 Size: 681 10 140 8 450 489 73 132 Cuts at: 9272 9604 10739 11420 11611 826 Size: 332 1135 681 191 1471 Fragments arranged by size: 1471 1135 1124 1074 1012 681 681 516 489 463 463 450 447 332 297 285 275 219 191 140 137 132 73 52 42 30 27 10 8 SfoI GGC'GCC Cuts at: 6008 6381 6601 6008 Size: 373 220 11663 Fragments arranged by size: 11663 373 220 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12256 SgrAI Cr'CCGG_yG Cuts at: 1700 6003 1700 Size: 4303 7953 SmaI CCC'GGG Cuts at: 1872 4527 5870 9583 1872 Size: 2655 1343 3713 4545 Fragments arranged by size: 4545 3713 2655 1343 SmlI C'TyrA_G Cuts at: 820 1017 1943 2307 4958 5683 6238 6264 7618 Size: 197 926 364 2651 725 555 26 1354 Cuts at: 7618 8801 10360 11261 11523 820 Size: 1183 1559 901 262 1553 Fragments arranged by size: 2651 1559 1553 1354 1183 926 901 725 555 364 262 197 26 SnaBI TAC'GTA Cuts at: 493 10047 493 Size: 9554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 12256 SphI G_CATG'C Cuts at: 1132 2024 4348 8272 9614 1132 Size: 892 2324 3924 1342 3774 Fragments arranged by size: 3924 3774 2324 1342 892 SrfI GCCC'GGGC Cuts at: 4527 4527 Size: 12256 SspI AAT'ATT Cuts at: 5 52 10180 5 Size: 47 10128 2081 Fragments arranged by size: 10128 2081 47 StuI AGG'CCT Cuts at: 1517 2092 2322 7703 9326 9371 9548 1517 Size: 575 230 5381 1623 45 177 4225 Fragments arranged by size: 5381 4225 1623 575 230 177 45 StyI C'CwwG_G Cuts at: 513 1065 2004 2778 3604 3903 7651 7872 8779 Size: 552 939 774 826 299 3748 221 907 Cuts at: 8779 8847 9695 513 Size: 68 848 3074 Fragments arranged by size: 3748 3074 939 907 848 826 774 552 299 221 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 3277 3581 3908 10283 10300 10453 Size: 1255 573 1124 304 327 6375 17 153 Cuts at: 10453 10638 11083 325 Size: 185 445 1498 Fragments arranged by size: 6375 1498 1255 1124 573 445 327 304 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6204 7772 9057 Size: 274 80 33 51 1443 4227 1568 1285 Cuts at: 9057 9084 10502 12214 96 Size: 27 1418 1712 138 Fragments arranged by size: 4227 1712 1568 1443 1418 1285 274 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3123 3155 3379 3411 4734 4766 4929 4961 5492 Size: 32 224 32 1323 32 163 32 531 Cuts at: 5492 5524 5918 5950 7526 7558 12216 12248 3123 Size: 32 394 32 1576 32 4658 32 3131 Fragments arranged by size: 4658 3131 1576 1323 531 394 224 163 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3832 1316 Size: 2516 9740 XbaI T'CTAG_A Cuts at: 9592 9592 Size: 12256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4835 7093 7748 4835 Size: 2258 655 9343 Fragments arranged by size: 9343 2258 655 XhoI C'TCGA_G Cuts at: 1943 2307 6264 1943 Size: 364 3957 7935 Fragments arranged by size: 7935 3957 364 XmaI C'CCGG_G Cuts at: 1870 4525 5868 9581 1870 Size: 2655 1343 3713 4545 Fragments arranged by size: 4545 3713 2655 1343 XmnI GAAnn'nnTTC Cuts at: 2079 5810 10385 2079 Size: 3731 4575 3950 Fragments arranged by size: 4575 3950 3731 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 10064 276 Size: 53 83 186 936 8530 2468 Fragments arranged by size: 8530 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BstZ17I FseI FspAI NruI PacI PspXI PsrI SacII SfiI SwaI