Restriction map for pFN21AB5654
 (Circular) (Six-base) MAPSORT of: pFN21AB5654.seq  Check: 7861  from: 1  to: 5698



pFN21AB5654  5698 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:31  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2152    3508     278 
   Size:         53      83     186     936     616    1356    2468

  Fragments arranged by size: 

                2468    1356     936     616     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2802    3041    1937 
   Size:        865     239    4594

  Fragments arranged by size: 

                4594     865     239
 
Acc65I G'GTAC_C

Cuts at:   3019    3019 
   Size:       5698
 
AclI AA'CG_TT

Cuts at:   1563    3825    4198    1563 
   Size:       2262     373    3063

  Fragments arranged by size: 

                3063    2262     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2409    2480    3761    5145    5413    1632 
   Size:        777      71    1281    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1281     777     268      71
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5501
 
AflIII A'CryG_T

Cuts at:   1203    2672    4597    5574    1203 
   Size:       1469    1925     977    1327

  Fragments arranged by size: 

                1925    1469    1327     977
 
AgeI A'CCGG_T

Cuts at:   4529    4529 
   Size:       5698
 
AhdI GACnn_n'nnGTC

Cuts at:   4427    4427 
   Size:       5698
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5698
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5698
 
AlwNI CAG_nnn'CTG

Cuts at:   2503    2750    3441    5013    2503 
   Size:        247     691    1572    3188

  Fragments arranged by size: 

                3188    1572     691     247
 
ApaI G_GGCC'C

Cuts at:   2446    2446 
   Size:       5698
 
ApaLI G'TGCA_C

Cuts at:   3755    4911    3755 
   Size:       1156    4542
 
ApoI r'AATT_y

Cuts at:   1493    3007    3233    3385    5494    1493 
   Size:       1514     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1514     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4252     160 
   Size:        872    3220    1606

  Fragments arranged by size: 

                3220    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5698
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3023    1870 
   Size:         73    1080    4545

  Fragments arranged by size: 

                4545    1080      73
 
BamHI G'GATC_C

Cuts at:   3028    3395    3028 
   Size:        367    5331
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3019    4474     618 
   Size:        325     217     627    1232    1455    1842

  Fragments arranged by size: 

                1842    1455    1232     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2446    3017    1391 
   Size:       1055     571    4072

  Fragments arranged by size: 

                4072    1055     571
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4727
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3889    3923    1903 
   Size:         34    1952      34    3678

  Fragments arranged by size: 

                3678    1952      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3590    4800    3590 
   Size:       1210    4488
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5698
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4309     136 
   Size:        107     122      71     631     605    2637    1525

  Fragments arranged by size: 

                2637    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5693    5693 
   Size:       5698
 
BlpI GC'TnA_GC

Cuts at:   3115    3115 
   Size:       5698
 
Bme1580I G_kGCm'C

Cuts at:   1790    2446    2497    3759    4915    1790 
   Size:        656      51    1262    1156    2573

  Fragments arranged by size: 

                2573    1262    1156     656      51
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5698
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2738    3429    4387 
   Size:        416      53      83     399      24    1321     691     958

Cuts at:   4387     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1321     958     691     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    2976    1056 
   Size:       1920    3778
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2216    2386    4358    1239 
   Size:        977     170    1972    2579

  Fragments arranged by size: 

                2579    1972     977     170
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3823    4688    4986    3823 
   Size:        865     298    4535

  Fragments arranged by size: 

                4535     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4361     882 
   Size:        617    2862    2219

  Fragments arranged by size: 

                2862    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3489     493 
   Size:        711    2285    2702

  Fragments arranged by size: 

                2702    2285     711
 
BsaBI GATnn'nnATC

Cuts at:   5692    5692 
   Size:       5698
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2149    3505    3887    4522 
   Size:         53      83     186     936     616    1356     382     635

Cuts at:   4522     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1356     936     635     616     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4131    4529    4803    4950    5361    4131 
   Size:        398     274     147     411    4468

  Fragments arranged by size: 

                4468     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5473

  Fragments arranged by size: 

                5473     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2029    1186 
   Size:        843    4855
 
BseYI C'CCAG_C

Cuts at:   2297    2421    2901    4901    5397    2297 
   Size:        124     480    2000     496    2598

  Fragments arranged by size: 

                2598    2000     496     480     124
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5125
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3148    3909    4937    1540 
   Size:        275     186    1147     761    1028    2301

  Fragments arranged by size: 

                2301    1147    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2028    2039    3759    3844    4915    2028 
   Size:         11    1720      85    1071    2811

  Fragments arranged by size: 

                2811    1720    1071      85      11
 
BsmI GAATG_Cn'

Cuts at:   2200    2217    3209    3302    2200 
   Size:         17     992      93    4596

  Fragments arranged by size: 

                4596     992      93      17
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2156    2156 
   Size:       5698
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2028    2039    2446    2497    3017    3759    3844 
   Size:        399     238      11     407      51     520     742      85

Cuts at:   3844    4915    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     520     407     399     238      85
                  51      11
 
BspEI T'CCGG_A

Cuts at:   5361    5361 
   Size:       5698
 
BspHI T'CATG_A

Cuts at:   2556    3587    5317    2556 
   Size:       1031    1730    2937

  Fragments arranged by size: 

                2937    1730    1031
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3053     844 
   Size:       2209    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3585    1394 
   Size:        429    1762    3507

  Fragments arranged by size: 

                3507    1762     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2551    4193    4367      65 
   Size:       1183    1303    1642     174    1396

  Fragments arranged by size: 

                1642    1396    1303    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4342    4529    1700 
   Size:        110    2532     187    2869

  Fragments arranged by size: 

                2869    2532     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5698
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5698
 
BssSI C'ACGA_G

Cuts at:   3758    4770    3758 
   Size:       1012    4686
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5698
 
BstBI TT'CG_AA

Cuts at:   4502    4502 
   Size:       5698
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5698
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2915    2915 
   Size:       5698
 
BstYI r'GATC_y

Cuts at:   1972    2065    3028    3395    3780    3797    5249    5335    5347 
   Size:         93     963     367     385      17    1452      86      12

Cuts at:   5347    5693    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     963     385     367     346      93      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2628     513 
   Size:        552    1563    3583

  Fragments arranged by size: 

                3583    1563     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4959

  Fragments arranged by size: 

                4959     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2052    3214    4008    4028    2052 
   Size:       1162     794      20    3722

  Fragments arranged by size: 

                3722    1162     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5698
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5663
 
DraI TTT'AAA

Cuts at:   3002    3002 
   Size:       5698
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4705     809 
   Size:        507    3389    1802

  Fragments arranged by size: 

                3389    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2730    3145    4034 
   Size:         54    1425      50     275      18     900     415     889

Cuts at:   4034    5663       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     900     889     415     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3145    1537 
   Size:        275    1333    4090

  Fragments arranged by size: 

                4090    1333     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3628    1353 
   Size:       2275    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4285    4659    4805     387 
   Size:       1064     138    2696     374     146    1280

  Fragments arranged by size: 

                2696    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2216    2386    2409    2480    3761    4358    5145 
   Size:        393     584     170      23      71    1281     597     787

Cuts at:   5145    5413    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1281     787     597     584     393     268     170
                  71      23
 
EcoO109I rG'GnC_Cy

Cuts at:   2442    2443    2442 
   Size:          1    5697
 
EcoRI G'AATT_C

Cuts at:   3007    3385    3007 
   Size:        378    5320
 
FspI TGC'GCA

Cuts at:   4204    4204 
   Size:       5698
 
HaeII r_GCGC'y

Cuts at:   1678    4845    1678 
   Size:       3167    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2139    2171    2697    2729    4339 
   Size:         32     135      32     567      32     526      32    1610

Cuts at:   4339    4371    4413    4445    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1610     567     526     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3042    3288     669 
   Size:        988     281    1104     246    3079

  Fragments arranged by size: 

                3079    1104     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5698
 
HpaI GTT'AAC

Cuts at:   3288    3288 
   Size:       5698
 
KpnI G_GTAC'C

Cuts at:   3023    3023 
   Size:       5698
 
MfeI C'AATT_G

Cuts at:   3297    3297 
   Size:       5698
 
MluI A'CGCG_T

Cuts at:   5574    5574 
   Size:       5698
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4812    4996    1305 
   Size:       3507     184    2007

  Fragments arranged by size: 

                3507    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2732      10 
   Size:         54    1425     343     900    2976

  Fragments arranged by size: 

                2976    1425     900     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3656    4015    4174     518 
   Size:        648    2490     359     159    2042

  Fragments arranged by size: 

                2490    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2458    2901    3114    3791    4939    5184    1606 
   Size:        852     443     213     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     852     677     443     245     213
 
NcoI C'CATG_G

Cuts at:    513    1065    2628     513 
   Size:        552    1563    3583

  Fragments arranged by size: 

                3583    1563     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5698
 
NheI G'CTAG_C

Cuts at:   1052    2972    1052 
   Size:       1920    3778
 
NotI GC'GGCC_GC

Cuts at:   3145    3145 
   Size:       5698
 
NspI r_CATG'y

Cuts at:   1132    1229    2676    3056    4601    1132 
   Size:         97    1447     380    1545    2229

  Fragments arranged by size: 

                2229    1545    1447     380      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5698
 
PciI A'CATG_T

Cuts at:   2672    4597    2672 
   Size:       1925    3773
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2304    2503    1108 
   Size:       1196     199    4303

  Fragments arranged by size: 

                4303    1196     199
 
PmeI GTTT'AAAC

Cuts at:   3002    3002 
   Size:       5698
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3767    3799    5280    5312    3767 
   Size:         32    1481      32    4153

  Fragments arranged by size: 

                4153    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5698
 
PsiI TTA'TAA

Cuts at:   3268    3268 
   Size:       5698
 
PspOMI G'GGCC_C

Cuts at:   2442    2442 
   Size:       5698
 
PstI C_TGCA'G

Cuts at:    830    2047    3050     830 
   Size:       1217    1003    3478

  Fragments arranged by size: 

                3478    1217    1003
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5698
 
PvuII CAG'CTG

Cuts at:   2458    2901    2458 
   Size:        443    5255
 
SalI G'TCGA_C

Cuts at:   1936    3040    1936 
   Size:       1104    4594
 
SbfI CC_TGCA'GG

Cuts at:   3050    3050 
   Size:       5698
 
ScaI AGT'ACT

Cuts at:   3946    5658    3946 
   Size:       1712    3986
 
SexAI A'CCwGG_T

Cuts at:   2502    2502 
   Size:       5698
 
SfcI C'TryA_G

Cuts at:    826    1045    2043    2788    3046    4181    4862    5053     826 
   Size:        219     998     745     258    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135     998     745     681     258     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5698
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5698
 
SmaI CCC'GGG

Cuts at:   1872    3025    1872 
   Size:       1153    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3802    4703    4965     820 
   Size:        197     926    1859     901     262    1553

  Fragments arranged by size: 

                1859    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3489     493 
   Size:       2996    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5698
 
SphI G_CATG'C

Cuts at:   1132    3056    1132 
   Size:       1924    3774
 
SspI AAT'ATT

Cuts at:      5      52    3622       5 
   Size:         47    3570    2081

  Fragments arranged by size: 

                3570    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5698
 
StyI C'CwwG_G

Cuts at:    513    1065    2628    3137     513 
   Size:        552    1563     509    3074

  Fragments arranged by size: 

                3074    1563     552     509
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3725    3742    3895    4080    4525     325 
   Size:       1255    2145      17     153     185     445    1498

  Fragments arranged by size: 

                2145    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2574    2682    2819 
   Size:        274      80      33      51    1443     597     108     137

Cuts at:   2819    2842    3944    5656      96 
   Size:         23    1102    1712     138

  Fragments arranged by size: 

                1712    1443    1102     597     274     138     137     108
                  80      51      33      23
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2466    2498    2677    2709    5658    5690    2466 
   Size:         32     179      32    2949      32    2474

  Fragments arranged by size: 

                2949    2474     179      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5698
 
XbaI T'CTAG_A

Cuts at:   3034    3034 
   Size:       5698
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2011    2011 
   Size:       5698
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5698
 
XmaI C'CCGG_G

Cuts at:   1870    3023    1870 
   Size:       1153    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3827    3827 
   Size:       5698
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2150    3506     276 
   Size:         53      83     186     936     616    1356    2468

  Fragments arranged by size: 

                2468    1356     936     616     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI     SfiI     SfoI
     SrfI     SwaI
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