Restriction map for
pFN21AB5559
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII AccII AccIII AciI AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HapII
HhaI HinP1I HincII HindII HindIII
HpaI HpaII ItaI KasI Ksp632I
KspI MaeI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TseI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 202 GGTACC G^GTACC
AccI 430 GTAGAC GT^[AC][GT]AC
AcsI 641 GAATTT [GA]^AATT[CT]
AcyI 3 GGCGTC G[GA]^CG[CT]C
ApoI 641 GAATTT [GA]^AATT[CT]
Asp718I 202 GGTACC G^GTACC
AspHI 187 GTGCTC G[AT]GC[AT]^C
BpmI 136 CTGGAG CTGGAG(16/14)
BsaBI 672 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsiHKAI 187 GTGCTC G[AT]GC[AT]^C
BsiYI 8 CCCCGTCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 8 CCCCGTCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 11 CGTCTC CGTCTC(1/5)
BspMI 244 ACCTGC ACCTGC(4/8)
BssKI 135 CCTGG ^CC[ACGT]GG
BssSI 701 CTCGTG CACGAG(-5/-1)
BstF5I 263 GGATG GGATG(2/0)
BstNI 135 CCTGG CC^[AT]GG
BstXI 666 CCAACTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 437 AGATCC [GA]^GATC[CT]
Cac8I 658 GCTTGC GC[ACGT]^[ACGT]GC
DraI 156 TTTAAA TTT^AAA
EcoRII 135 CCTGG ^CC[AT]GG
FokI 263 GGATG GGATG(9/13)
HaeIII 168 GGCC GG^CC
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
KpnI 202 GGTACC GGTAC^C
MaeII 448 ACGT A^CGT
MaeIII 466 GTCAC ^GT[ACGT]AC
MamI 672 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 541 GAAGA GAAGA(8/7)
MflI 437 AGATCC [GA]^GATC[CT]
MnlI 696 CCTC CCTC(7/6)
MslI 454 CATTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 135 CCTGG CC^[AT]GG
PstI 22 CTGCAG CTGCA^G
ScrFI 135 CCTGG CC^[ACGT]GG
TaqI 308 TCGA T^CGA
TfiI 677 GAATC G^A[AT]TC
Tsp45I 466 GTCAC ^GT[GC]AC
TspRI 90 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 308 TCGA T^CGA
XhoII 437 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 203 GTAC GT^AC
548 GTAC
AluI 252 AGCT AG^CT
361 AGCT
AlwI 438 GATCC GGATC(4/5)
86 GGATC
BanI 202 GGTACC G^G[CT][GA]CC
223 GGCACC
BmyI 187 GTGCTC G[AGT]GC[ACT]^C
222 GGGCAC
BsmAI 18 GAGAC GTCTC(1/5)
12 GTCTC
BsmFI 6 GTCCC GGGAC(10/14)
178 GTCCC
Bsp1286I 187 GTGCTC G[AGT]GC[ACT]^C
222 GGGCAC
BsrDI 192 CATTGC GCAATG(2/0)
280 GCAATG
DdeI 504 CTCAG C^T[ACGT]AG
521 CTAAG
DpnII 87 GATC ^GATC
438 GATC
MboI 87 GATC ^GATC
438 GATC
MwoI 116 GCATTTGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
125 GCAAGGTGAGC
NdeII 87 GATC ^GATC
438 GATC
NlaIV 202 GGTACC GG[ACGT]^[ACGT]CC
223 GGCACC
RsaI 203 GTAC GT^AC
548 GTAC
Sau3AI 87 GATC ^GATC
438 GATC
SfaNI 264 GATGC GCATC(5/9)
562 GATGC
SfcI 22 CTGCAG C^T[GA][CT]AG
404 CTACAG
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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BsrI HinfI HphI MseI NlaIII
PleI Tru9I Tsp509I