Restriction map for
pFN21AB5464
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HapII HincII HindII
HindIII HpaI HpaII KasI KspI
MluI MroI MspI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 132 GGTACC G^GTACC
AccII 102 CGCG CG^CG
AcyI 94 GACGCC G[GA]^CG[CT]C
AflIII 254 ACGTGT A^C[GA][CT]GT
AluI 324 AGCT AG^CT
Asp718I 132 GGTACC G^GTACC
AspHI 601 GAGCAC G[AT]GC[AT]^C
AvaII 117 GGACC G^G[AT]CC
BalI 141 TGGCCA TGG^CCA
BanI 132 GGTACC G^G[CT][GA]CC
BbvI 325 GCTGC GCAGC(8/12)
BcgI 737 GCAATTGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 636 TGATCA T^GATCA
BglII 471 AGATCT A^GATCT
BpmI 541 CTGGAG CTGGAG(16/14)
BsaBI 415 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 94 GACGCC G[GA]^CG[CT]C
BsiHKAI 601 GAGCAC G[AT]GC[AT]^C
BsmFI 400 GTCCC GGGAC(10/14)
Bsp1407I 224 TGTACA T^GTACA
BspMI 196 GCAGGT ACCTGC(4/8)
BsrGI 224 TGTACA T^GTACA
BstF5I 84 CATCC GGATG(2/0)
BstUI 102 CGCG CG^CG
BstYI 471 AGATCT [GA]^GATC[CT]
CfoI 76 GCGC GCG^C
DdeI 581 CTCAG C^T[ACGT]AG
DraII 116 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 776 CCGTGG C^C[GA][CT]GG
EaeI 141 TGGCCA [CT]^GGCC[GA]
EarI 149 GAAGAG CTCTTC(1/4)
EcoO109I 116 AGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 636 TGATCA T^GATCA
FokI 84 CATCC GGATG(9/13)
HaeII 75 GGCGCT [GA]GCGC^[CT]
HgaI 94 GACGC GACGC(5/10)
HhaI 76 GCGC GCG^C
Hin1I 94 GACGCC G[GA]^CG[CT]C
HinP1I 76 GCGC G^CGC
HinfI 594 GATTC G^A[ACGT]TC
KpnI 132 GGTACC GGTAC^C
Ksp632I 149 GAAGAG CTCTTC(1/4)
MamI 415 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 471 AGATCT [GA]^GATC[CT]
MluNI 141 TGGCCA TGG^CCA
MscI 141 TGGCCA TGG^CCA
MslI 516 CATTGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 323 CAGCTG C[AC]G^C[GT]G
MvnI 102 CGCG CG^CG
MwoI 319 GCCCCAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 167 GCATGT [GA]CATG^[CT]
PpuMI 116 AGGACCC [GA]G^G[AT]CC[CT]
PstI 311 CTGCAG CTGCA^G
PvuII 323 CAGCTG CAG^CTG
SapI 149 GAAGAGC GCTCTTC(1/4)
SfaNI 316 GATGC GCATC(5/9)
SfcI 311 CTGCAG C^T[GA][CT]AG
SspBI 224 TGTACA T^GTACA
TaqI 660 TCGA T^CGA
TfiI 594 GATTC G^A[AT]TC
TseI 325 GCTGC G^C[AT]GC
Tsp45I 10 GTGAC ^GT[GC]AC
TspRI 390 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 660 TCGA T^CGA
XcmI 530 CCAGAAACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 471 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 382 GAATTT [GA]^AATT[CT]
769 AAATTT
AlwI 421 GATCC GGATC(4/5)
498 GATCC
ApoI 382 GAATTT [GA]^AATT[CT]
769 AAATTT
AspI 94 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
662 GACTGTGTC
BanII 55 GAGCCC G[GA]GC[CT]^C
304 GAGCCC
BfaI 181 CTAG C^TAG
208 CTAG
BsaJI 642 CCTGGG C^C[ACGT][ACGT]GG
776 CCGTGG
BseRI 70 GAGGAG GAGGAG(10/8)
212 GAGGAG
BsrBI 23 CCGCTC CCGCTC(-3/-3)
63 GAGCGG
BsrI 540 ACTGG ACTGG(1/-1)
136 CCAGT
Cac8I 192 GCTTGC GC[ACGT]^[ACGT]GC
733 GCCAGC
Cfr13I 30 GGCCC G^G[ACGT]CC
117 GGACC
Fnu4HI 65 GCGGC GC^[ACGT]GC
325 GCTGC
ItaI 65 GCGGC GC^[ACGT]GC
325 GCTGC
MaeI 181 CTAG C^TAG
208 CTAG
MaeIII 10 GTGAC ^GT[ACGT]AC
546 GTTAC
MfeI 341 CAATTG C^AATTG
738 CAATTG
MunI 341 CAATTG C^AATTG
738 CAATTG
PflFI 94 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
662 GACTGTGTC
Sau96I 30 GGCCC G^G[ACGT]CC
117 GGACC
SspI 230 AATATT AAT^ATT
352 AATATT
Tth111I 94 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
662 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AfaI BmyI BsiYI BslI
Bsp1286I BspHI BssKI BstNI DpnII
EcoRII HaeIII HphI MaeII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV RcaI RsaI Sau3AI
ScrFI Tru9I Tsp509I