Restriction map for
pFN21AB5419
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrI
BssHII BssSI BstAPI BstBI BstUI
BstYI Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV EcoT22I
FbaI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HpaI
HpaII KasI Ksp632I KspI MfeI
MflI MluI MroI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 774 GGTACC G^GTACC
AccI 532 GTATAC GT^[AC][GT]AC
AciI 330 GCGG CCGC(-3/-1)
AlwI 885 GATCC GGATC(4/5)
Asp718I 774 GGTACC G^GTACC
AvaII 318 GGACC G^G[AT]CC
BanI 774 GGTACC G^G[CT][GA]CC
BfaI 160 CTAG C^TAG
BsaBI 555 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 433 CCCATGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 433 CCCATGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 810 GTCCC GGGAC(10/14)
Bsp1407I 791 TGTACA T^GTACA
BspHI 512 TCATGA T^CATGA
BsrDI 174 CATTGC GCAATG(2/0)
BsrGI 791 TGTACA T^GTACA
Bst1107I 532 GTATAC GTA^TAC
BstEII 360 GGTCACC G^GT[ACGT]ACC
BstPI 360 GGTCACC G^GT[ACGT]ACC
BstXI 308 CCATCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 532 GTATAC GTA^TAC
DpnII 885 GATC ^GATC
DrdI 681 GACGGCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 50 CCATGG C^C[GA][CT]GG
EcoO65I 360 GGTCACC G^GT[ACGT]ACC
EcoRI 945 GAATTC G^AATTC
EcoT14I 50 CCATGG C^C[AT][AT]GG
FauI 330 GCGGG CCCGC(4/6)
HindIII 337 AAGCTT A^AGCTT
KpnI 774 GGTACC GGTAC^C
MaeI 160 CTAG C^TAG
MaeII 89 ACGT A^CGT
MamI 555 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 885 GATC ^GATC
MseI 764 TTAA T^TAA
MspA1I 243 CAGCTG C[AC]G^C[GT]G
NcoI 50 CCATGG C^CATGG
NdeII 885 GATC ^GATC
NlaIV 774 GGTACC GG[ACGT]^[ACGT]CC
PleI 68 GACTC GAGTC(4/5)
PstI 127 CTGCAG CTGCA^G
PvuII 243 CAGCTG CAG^CTG
RcaI 512 TCATGA T^CATGA
Sau3AI 885 GATC ^GATC
SspBI 791 TGTACA T^GTACA
StyI 50 CCATGG C^C[AT][AT]GG
TfiI 919 GATTC G^A[AT]TC
Tru9I 764 TTAA T^TAA
TspRI 15 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 14 enzymes) #
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AcsI 56 AAATTT [GA]^AATT[CT]
945 GAATTC
ApoI 56 AAATTT [GA]^AATT[CT]
945 GAATTC
BbvI 245 GCTGC GCAGC(8/12)
729 GCTGC
BsmAI 649 GAGAC GTCTC(1/5)
154 GTCTC
CfoI 74 GCGC GCG^C
581 GCGC
Fnu4HI 245 GCTGC GC^[ACGT]GC
729 GCTGC
HhaI 74 GCGC GCG^C
581 GCGC
HinP1I 74 GCGC G^CGC
581 GCGC
HinfI 68 GACTC G^A[ACGT]TC
919 GATTC
ItaI 245 GCTGC GC^[ACGT]GC
729 GCTGC
MboII 374 GAAGA GAAGA(8/7)
942 GAAGA
SfcI 127 CTGCAG C^T[GA][CT]AG
240 CTACAG
TseI 245 GCTGC G^C[AT]GC
729 GCTGC
Tsp45I 361 GTCAC ^GT[GC]AC
481 GTCAC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI BalI BsaJI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EaeI EcoRII FokI HaeIII HphI
MaeIII MluNI MnlI MscI MslI
MvaI MwoI NlaIII RsaI Sau96I
ScrFI SfaNI Tsp509I