Restriction map for
pFN21AB5375
(Circular) (Six-base) MAPSORT of: pFN21AB5375.seq Check: 650 from: 1 to: 5380
pFN21AB5375 5380 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3190 278
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2723 1937
Size: 786 4594
Acc65I G'GTAC_C
Cuts at: 2701 2701
Size: 5380
AclI AA'CG_TT
Cuts at: 1563 3507 3880 1563
Size: 1944 373 3063
Fragments arranged by size:
3063 1944 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2307 2405 2579 3443 4827 5095 1632
Size: 675 98 174 864 1384 268 1917
Fragments arranged by size:
1917 1384 864 675 268 174 98
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5183
AflIII A'CryG_T
Cuts at: 1203 2115 2271 4279 5256 1203
Size: 912 156 2008 977 1327
Fragments arranged by size:
2008 1327 977 912 156
AgeI A'CCGG_T
Cuts at: 4211 4211
Size: 5380
AhdI GACnn_n'nnGTC
Cuts at: 4109 4109
Size: 5380
AleI CACnn'nnGTG
Cuts at: 1166 2480 1166
Size: 1314 4066
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5380
AlwNI CAG_nnn'CTG
Cuts at: 3123 4695 3123
Size: 1572 3808
ApaLI G'TGCA_C
Cuts at: 3437 4593 3437
Size: 1156 4224
ApoI r'AATT_y
Cuts at: 1493 2165 2447 2689 2915 3067 5176 1493
Size: 672 282 242 226 152 2109 1697
Fragments arranged by size:
2109 1697 672 282 242 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3934 160
Size: 872 2902 1606
Fragments arranged by size:
2902 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2705 1870
Size: 73 87 675 4545
Fragments arranged by size:
4545 675 87 73
BamHI G'GATC_C
Cuts at: 2710 3077 2710
Size: 367 5013
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2701 4156 618
Size: 325 217 627 914 1455 1842
Fragments arranged by size:
1842 1455 914 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2699 1391
Size: 1308 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3571 3605 1903
Size: 34 1634 34 3678
Fragments arranged by size:
3678 1634 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2083 3272 4482 2083
Size: 1189 1210 2981
Fragments arranged by size:
2981 1210 1189
BclI T'GATC_A
Cuts at: 1548 2617 1548
Size: 1069 4311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3991 136
Size: 107 122 71 631 605 2319 1525
Fragments arranged by size:
2319 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5375 5375
Size: 5380
BlpI GC'TnA_GC
Cuts at: 2797 2797
Size: 5380
Bme1580I G_kGCm'C
Cuts at: 1790 3441 4597 1790
Size: 1651 1156 2573
Fragments arranged by size:
2573 1651 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5380
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2047 2650 3111
Size: 416 53 83 399 24 630 603 461
Cuts at: 3111 4069 442
Size: 958 1753
Fragments arranged by size:
1753 958 630 603 461 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 4040 1239
Size: 860 1941 2579
Fragments arranged by size:
2579 1941 860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3505 4370 4668 3505
Size: 865 298 4217
Fragments arranged by size:
4217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4043 882
Size: 617 2544 2219
Fragments arranged by size:
2544 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2118 3171 493
Size: 711 914 1053 2702
Fragments arranged by size:
2702 1053 914 711
BsaBI GATnn'nnATC
Cuts at: 2636 5374 2636
Size: 2738 2642
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3187 3569 4204 275
Size: 53 83 186 936 1654 382 635 1451
Fragments arranged by size:
1654 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3813 4211 4485 4632 5043 3813
Size: 398 274 147 411 4150
Fragments arranged by size:
4150 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 1178
Size: 30 165 30 693 30 4432
Fragments arranged by size:
4432 693 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 1186
Size: 931 4449
BseYI C'CCAG_C
Cuts at: 4583 5079 4583
Size: 496 4884
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4807
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2830 3591 4619 1540
Size: 275 186 829 761 1028 2301
Fragments arranged by size:
2301 1028 829 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3441 3526 4597 3441
Size: 85 1071 4224
Fragments arranged by size:
4224 1071 85
BsmI GAATG_Cn'
Cuts at: 2891 2984 2891
Size: 93 5287
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2699 3441 3526 4597 1391
Size: 399 909 742 85 1071 2174
Fragments arranged by size:
2174 1071 909 742 399 85
BspEI T'CCGG_A
Cuts at: 5043 5043
Size: 5380
BspHI T'CATG_A
Cuts at: 3269 4999 3269
Size: 1730 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2735 844
Size: 1891 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3267 1394
Size: 429 1444 3507
Fragments arranged by size:
3507 1444 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2242 3875 4049 65
Size: 1183 994 1633 174 1396
Fragments arranged by size:
1633 1396 1183 994 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4024 4211 1700
Size: 110 2214 187 2869
Fragments arranged by size:
2869 2214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5380
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5380
BssSI C'ACGA_G
Cuts at: 3440 4452 3440
Size: 1012 4368
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5380
BstBI TT'CG_AA
Cuts at: 4184 4184
Size: 5380
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5380
BstXI CCAn_nnnn'nTGG
Cuts at: 2440 2539 2440
Size: 99 5281
BstYI r'GATC_y
Cuts at: 1972 2710 3077 3462 3479 4931 5017 5029 5375
Size: 738 367 385 17 1452 86 12 346
Cuts at: 5375 1972
Size: 1977
Fragments arranged by size:
1977 1452 738 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4828
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2239 487
Size: 683 56 1013 3628
Fragments arranged by size:
3628 1013 683 56
BtsI GCAGTG_nn'
Cuts at: 2896 3690 3710 2896
Size: 794 20 4566
Fragments arranged by size:
4566 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2257 2292 529
Size: 35 1693 35 3617
Fragments arranged by size:
3617 1693 35 35
DraI TTT'AAA
Cuts at: 2684 2684
Size: 5380
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4387 809
Size: 507 3071 1802
Fragments arranged by size:
3071 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2827 3716 5345
Size: 54 1425 50 275 18 997 889 1629
Cuts at: 5345 8
Size: 43
Fragments arranged by size:
1629 1425 997 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2827 1537
Size: 275 1015 4090
Fragments arranged by size:
4090 1015 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2397 3310 1353
Size: 1044 913 3423
Fragments arranged by size:
3423 1044 913
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2057 3967 4341 4487 387
Size: 1064 138 468 1910 374 146 1280
Fragments arranged by size:
1910 1280 1064 468 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2307 2405 2579 3443 4040 4827
Size: 393 467 208 98 174 864 597 787
Cuts at: 4827 5095 1239
Size: 268 1524
Fragments arranged by size:
1524 864 787 597 467 393 268 208
174 98
EcoRI G'AATT_C
Cuts at: 2689 3067 2689
Size: 378 5002
EcoRV GAT'ATC
Cuts at: 2503 2503
Size: 5380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 5380
FspI TGC'GCA
Cuts at: 3886 3886
Size: 5380
HaeII r_GCGC'y
Cuts at: 1678 4527 1678
Size: 2849 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2295 2327 2534 2566 4021
Size: 32 135 32 723 32 207 32 1455
Cuts at: 4021 4053 4095 4127 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1455 723 207 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2724 2970 669
Size: 988 281 786 246 3079
Fragments arranged by size:
3079 988 786 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5380
HpaI GTT'AAC
Cuts at: 2970 2970
Size: 5380
KpnI G_GTAC'C
Cuts at: 2705 2705
Size: 5380
MfeI C'AATT_G
Cuts at: 2979 2979
Size: 5380
MluI A'CGCG_T
Cuts at: 5256 5256
Size: 5380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4494 4678 1305
Size: 3189 184 2007
Fragments arranged by size:
3189 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3558
Fragments arranged by size:
3558 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 3338 3697 3856 518
Size: 648 1314 858 359 159 2042
Fragments arranged by size:
2042 1314 858 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2796 3473 4621 4866 1606
Size: 1190 677 1148 245 2120
Fragments arranged by size:
2120 1190 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4828
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5380
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5380
NotI GC'GGCC_GC
Cuts at: 2827 2827
Size: 5380
NspI r_CATG'y
Cuts at: 1132 1229 2275 2738 4283 1132
Size: 97 1046 463 1545 2229
Fragments arranged by size:
2229 1545 1046 463 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5380
PciI A'CATG_T
Cuts at: 2271 4279 2271
Size: 2008 3372
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2155 2329 1108
Size: 1047 174 4159
Fragments arranged by size:
4159 1047 174
PfoI T'CCnGG_A
Cuts at: 2076 2205 2076
Size: 129 5251
PmeI GTTT'AAAC
Cuts at: 2684 2684
Size: 5380
PmlI CAC'GTG
Cuts at: 2118 2118
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 4962 4994 3449
Size: 32 1481 32 3835
Fragments arranged by size:
3835 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5380
PsiI TTA'TAA
Cuts at: 2950 2950
Size: 5380
PstI C_TGCA'G
Cuts at: 830 2732 830
Size: 1902 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5380
SalI G'TCGA_C
Cuts at: 1936 2722 1936
Size: 786 4594
SapI GCTCTTCn'nnn_
Cuts at: 2397 2397
Size: 5380
SbfI CC_TGCA'GG
Cuts at: 2732 2732
Size: 5380
ScaI AGT'ACT
Cuts at: 2396 3628 5340 2396
Size: 1232 1712 2436
Fragments arranged by size:
2436 1712 1232
SfcI C'TryA_G
Cuts at: 826 1045 2728 3863 4544 4735 826
Size: 219 1683 1135 681 191 1471
Fragments arranged by size:
1683 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5380
SmaI CCC'GGG
Cuts at: 1872 2707 1872
Size: 835 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3484 4385 4647 820
Size: 197 926 1541 901 262 1553
Fragments arranged by size:
1553 1541 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3171 493
Size: 2678 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5380
SphI G_CATG'C
Cuts at: 1132 2738 1132
Size: 1606 3774
SspI AAT'ATT
Cuts at: 5 52 3304 5
Size: 47 3252 2081
Fragments arranged by size:
3252 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5380
StyI C'CwwG_G
Cuts at: 513 1065 2819 513
Size: 552 1754 3074
Fragments arranged by size:
3074 1754 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2243 3407 3424 3577 3762 4207 325
Size: 1255 663 1164 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1164 663 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2394 3626 5338
Size: 274 80 33 51 1443 417 1232 1712
Cuts at: 5338 96
Size: 138
Fragments arranged by size:
1712 1443 1232 417 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5340 5372 5340
Size: 32 5348
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5380
XbaI T'CTAG_A
Cuts at: 2716 2716
Size: 5380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5380
XmaI C'CCGG_G
Cuts at: 1870 2705 1870
Size: 835 4545
XmnI GAAnn'nnTTC
Cuts at: 2298 2426 2564 3509 2298
Size: 128 138 945 4169
Fragments arranged by size:
4169 945 138 128
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3188 276
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI