(Circular) (Six-base) MAPSORT of: pFN21AB5289.seq Check: 1639 from: 1 to: 5959 pFN21AB5289 5959 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 2363 3280 2363 Size: 917 5042 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2939 2975 4022 5406 5674 1632 Size: 1307 36 1047 1384 268 1917 Fragments arranged by size: 1917 1384 1307 1047 268 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 2014 2058 2174 3702 5274 2014 Size: 44 116 1528 1572 2699 Fragments arranged by size: 2699 1572 1528 116 44 ApaI G_GGCC'C Cuts at: 3220 3257 3220 Size: 37 5922 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 2598 2857 3268 3494 3646 5755 1493 Size: 1105 259 411 226 152 2109 1697 Fragments arranged by size: 2109 1697 1105 411 259 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2045 2687 3284 1870 Size: 73 102 642 597 4545 Fragments arranged by size: 4545 642 597 102 73 BamHI G'GATC_C Cuts at: 2134 3289 3656 2134 Size: 1155 367 4437 Fragments arranged by size: 4437 1155 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2363 2470 3280 4735 618 Size: 325 217 627 576 107 810 1455 1842 Fragments arranged by size: 1842 1455 810 627 576 325 217 107 BanII G_rGCy'C Cuts at: 1391 2041 2093 2225 2326 2379 3220 3257 3278 Size: 650 52 132 101 53 841 37 21 Cuts at: 3278 1391 Size: 4072 Fragments arranged by size: 4072 841 650 132 101 53 52 37 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2927 928 Size: 971 1028 3960 Fragments arranged by size: 3960 1028 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2218 2252 4150 4184 1903 Size: 34 50 34 197 34 1898 34 3678 Fragments arranged by size: 3678 1898 197 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 2340 1548 Size: 792 5167 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2273 4570 Size: 107 122 71 631 605 337 264 2297 Cuts at: 4570 136 Size: 1525 Fragments arranged by size: 2297 1525 631 605 337 264 122 107 71 BglII A'GATC_T Cuts at: 2776 5954 2776 Size: 3178 2781 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 2052 3220 3257 4020 5176 1790 Size: 262 1168 37 763 1156 2573 Fragments arranged by size: 2573 1168 1156 763 262 37 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2560 3690 4648 Size: 416 53 83 399 24 1143 1130 958 Cuts at: 4648 442 Size: 1753 Fragments arranged by size: 1753 1143 1130 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2060 2107 2060 Size: 47 5912 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2656 4619 1239 Size: 1417 1963 2579 Fragments arranged by size: 2579 1963 1417 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2396 4084 4949 5247 2396 Size: 1688 865 298 3108 Fragments arranged by size: 3108 1688 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 2775 5953 2775 Size: 3178 2781 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3766 4148 4783 275 Size: 53 83 186 936 2233 382 635 1451 Fragments arranged by size: 2233 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2810 4392 4790 5064 5211 5622 2810 Size: 1582 398 274 147 411 3147 Fragments arranged by size: 3147 1582 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5734 Fragments arranged by size: 5734 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 2033 2099 2141 2144 2238 2888 1186 Size: 829 18 66 42 3 94 650 4257 Fragments arranged by size: 4257 829 650 94 66 42 18 3 BseYI C'CCAG_C Cuts at: 2284 2796 3122 5162 5658 2284 Size: 512 326 2040 496 2585 Fragments arranged by size: 2585 2040 512 496 326 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2576 2948 1163 Size: 573 840 372 4174 Fragments arranged by size: 4174 840 573 372 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2241 2379 4020 4105 5176 2241 Size: 138 1641 85 1071 3024 Fragments arranged by size: 3024 1641 1071 138 85 BsmI GAATG_Cn' Cuts at: 2616 3470 3563 2616 Size: 854 93 5012 Fragments arranged by size: 5012 854 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2052 2093 2225 2241 2326 2379 Size: 399 251 11 41 132 16 85 53 Cuts at: 2379 3220 3257 3278 4020 4105 5176 1391 Size: 841 37 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 841 742 399 251 132 85 85 53 41 37 21 16 11 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 2899 3848 5578 2899 Size: 949 1730 3280 Fragments arranged by size: 3280 1730 949 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2541 3314 844 Size: 1697 773 3489 Fragments arranged by size: 3489 1697 773 BsrBI CCG'CTC Cuts at: 1394 1823 2291 3846 1394 Size: 429 468 1555 3507 Fragments arranged by size: 3507 1555 468 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4454 4628 65 Size: 1183 3206 174 1396 Fragments arranged by size: 3206 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4603 4790 1700 Size: 110 2793 187 2869 Fragments arranged by size: 2869 2793 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstXI CCAn_nnnn'nTGG Cuts at: 2467 2467 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2134 2776 3289 3656 4041 4058 5510 5596 Size: 162 642 513 367 385 17 1452 86 Cuts at: 5596 5608 5954 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 642 513 385 367 346 162 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2745 2745 Size: 5959 BtgI C'CryG_G Cuts at: 513 1065 2262 2359 513 Size: 552 1197 97 4113 Fragments arranged by size: 4113 1197 552 97 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 2391 2657 3475 4269 4289 2391 Size: 266 818 794 20 4061 Fragments arranged by size: 4061 818 794 266 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 3406 4295 Size: 54 1425 50 275 18 429 1147 889 Cuts at: 4295 5924 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1147 889 429 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2703 3230 3889 1353 Size: 1350 527 659 3423 Fragments arranged by size: 3423 1350 659 527 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2377 2377 Size: 5959 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2656 2939 2975 4022 4619 5406 5674 Size: 393 1024 283 36 1047 597 787 268 Cuts at: 5674 1239 Size: 1524 Fragments arranged by size: 1524 1047 1024 787 597 393 283 268 36 EcoO109I rG'GnC_Cy Cuts at: 2011 2725 3216 3217 3253 3254 2011 Size: 714 491 1 36 1 4716 Fragments arranged by size: 4716 714 491 36 1 1 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FspI TGC'GCA Cuts at: 2450 4465 2450 Size: 2015 3944 HaeII r_GCGC'y Cuts at: 1678 2120 5106 1678 Size: 442 2986 2531 Fragments arranged by size: 2986 2531 442 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4600 4632 4674 4706 1373 Size: 32 135 32 3028 32 42 32 2626 Fragments arranged by size: 3028 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3549 669 Size: 988 281 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 2367 3284 2367 Size: 917 5042 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2205 2718 5073 5257 1305 Size: 900 513 2355 184 2007 Fragments arranged by size: 2355 2007 900 513 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 10 Size: 54 1425 343 429 3708 Fragments arranged by size: 3708 1425 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3917 4276 4435 518 Size: 648 2751 359 159 2042 Fragments arranged by size: 2751 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2171 2784 2800 3160 3375 4052 5200 Size: 452 113 613 16 360 215 677 1148 Cuts at: 5200 5445 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 613 452 360 245 215 113 16 NcoI C'CATG_G Cuts at: 513 1065 2359 513 Size: 552 1294 4113 Fragments arranged by size: 4113 1294 552 NdeI CA'TA_TG Cuts at: 387 2488 387 Size: 2101 3858 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3317 4862 1132 Size: 97 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 97 PasI CC'CwG_GG Cuts at: 1348 3128 3249 1348 Size: 1780 121 4058 Fragments arranged by size: 4058 1780 121 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PfoI T'CCnGG_A Cuts at: 2423 2423 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2725 2725 Size: 5959 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PspOMI G'GGCC_C Cuts at: 3216 3253 3216 Size: 37 5922 PspXI vC'TCGA_Gb Cuts at: 2687 2687 Size: 5959 PstI C_TGCA'G Cuts at: 830 2994 3138 3311 830 Size: 2164 144 173 3478 Fragments arranged by size: 3478 2164 173 144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 2058 2784 2800 2058 Size: 726 16 5217 Fragments arranged by size: 5217 726 16 SacI G_AGCT'C Cuts at: 2379 2379 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SanDI GG'GwC_CC Cuts at: 2725 2725 Size: 5959 SbfI CC_TGCA'GG Cuts at: 2994 3311 2994 Size: 317 5642 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2990 3134 3307 4442 5123 5314 826 Size: 219 1945 144 173 1135 681 191 1471 Fragments arranged by size: 1945 1471 1135 681 219 191 173 144 SfiI GGCCn_nnn'nGGCC Cuts at: 2273 2273 Size: 5959 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 2687 4063 4964 5226 820 Size: 197 926 468 276 1376 901 262 1553 Fragments arranged by size: 1553 1376 926 901 468 276 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2359 3398 513 Size: 552 1294 1039 3074 Fragments arranged by size: 3074 1294 1039 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2542 2925 3986 4003 4156 4341 4786 Size: 1255 962 383 1061 17 153 185 445 Cuts at: 4786 325 Size: 1498 Fragments arranged by size: 1498 1255 1061 962 445 383 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4205 5917 96 Size: 274 80 33 51 1443 2228 1712 138 Fragments arranged by size: 2228 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5919 5951 5919 Size: 32 5927 Tth111I GACn'n_nGTC Cuts at: 1316 2387 1316 Size: 1071 4888 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 2687 1943 Size: 744 5215 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 2680 4088 2680 Size: 1408 4551 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SapI SexAI SfoI SrfI SwaI