(Circular) (Six-base) MAPSORT of: pFN21AB5278.seq Check: 3204 from: 1 to: 5830 pFN21AB5278 5830 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2733 3173 1937 Size: 796 440 4594 Fragments arranged by size: 4594 796 440 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2262 3893 5277 5545 1632 Size: 630 1631 1384 268 1917 Fragments arranged by size: 1917 1631 1384 630 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 4729 5706 1203 Size: 3526 977 1327 Fragments arranged by size: 3526 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 2786 2943 1166 Size: 1620 157 4053 Fragments arranged by size: 4053 1620 157 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2051 1184 Size: 867 4963 AlwNI CAG_nnn'CTG Cuts at: 3573 5145 3573 Size: 1572 4258 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 2566 3139 3365 3517 5626 1493 Size: 1073 573 226 152 2109 1697 Fragments arranged by size: 2109 1697 1073 573 226 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 2633 2784 3155 1870 Size: 73 690 151 371 4545 Fragments arranged by size: 4545 690 371 151 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2639 3151 4606 618 Size: 325 217 627 852 512 1455 1842 Fragments arranged by size: 1842 1455 852 627 512 325 217 BanII G_rGCy'C Cuts at: 1391 2802 3149 1391 Size: 1411 347 4072 Fragments arranged by size: 4072 1411 347 BbeI G_GCGC'C Cuts at: 2643 2643 Size: 5830 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4859 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3109 3143 4021 4055 1903 Size: 34 1172 34 878 34 3678 Fragments arranged by size: 3678 1172 878 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 2409 1548 Size: 861 4969 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2886 4441 136 Size: 107 122 71 631 605 1214 1555 1525 Fragments arranged by size: 1555 1525 1214 631 605 122 107 71 BglII A'GATC_T Cuts at: 5825 5825 Size: 5830 BlpI GC'TnA_GC Cuts at: 3247 3247 Size: 5830 Bme1580I G_kGCm'C Cuts at: 1790 2793 3891 5047 1790 Size: 1003 1098 1156 2573 Fragments arranged by size: 2573 1156 1098 1003 BmgBI CAC'GTC Cuts at: 1318 2599 1318 Size: 1281 4549 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2072 3561 4519 Size: 416 53 83 399 24 655 1489 958 Cuts at: 4519 442 Size: 1753 Fragments arranged by size: 1753 1489 958 655 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2042 1056 Size: 986 4844 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2134 2134 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 4490 1239 Size: 848 2403 2579 Fragments arranged by size: 2579 2403 848 Bpu10I CC'TnA_GC Cuts at: 2655 2655 Size: 5830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3955 4820 5118 3955 Size: 865 298 4667 Fragments arranged by size: 4667 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3008 4493 882 Size: 617 1509 1485 2219 Fragments arranged by size: 2219 1509 1485 617 BsaAI yAC'GTr Cuts at: 493 1204 3621 493 Size: 711 2417 2702 Fragments arranged by size: 2702 2417 711 BsaBI GATnn'nnATC Cuts at: 2813 5824 2813 Size: 3011 2819 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2640 3637 4019 4654 Size: 53 83 186 936 1107 997 382 635 Cuts at: 4654 275 Size: 1451 Fragments arranged by size: 1451 1107 997 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2085 2115 1178 Size: 30 165 30 682 30 4893 Fragments arranged by size: 4893 682 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 1186 Size: 940 4890 BseYI C'CCAG_C Cuts at: 5033 5529 5033 Size: 496 5334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2987 1163 Size: 573 1251 4006 Fragments arranged by size: 4006 1251 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 4041 5069 1540 Size: 275 186 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2987 3891 3976 5047 2987 Size: 904 85 1071 3770 Fragments arranged by size: 3770 1071 904 85 BsmI GAATG_Cn' Cuts at: 3341 3434 3341 Size: 93 5737 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2793 2802 2987 3149 3891 3976 5047 Size: 399 1003 9 185 162 742 85 1071 Cuts at: 5047 1391 Size: 2174 Fragments arranged by size: 2174 1071 1003 742 399 185 162 85 9 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3185 844 Size: 2341 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3717 1394 Size: 429 1894 3507 Fragments arranged by size: 3507 1894 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2503 4325 4499 65 Size: 1183 1255 1822 174 1396 Fragments arranged by size: 1822 1396 1255 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2690 4474 4661 1700 Size: 110 880 1784 187 2869 Fragments arranged by size: 2869 1784 880 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 3890 4902 3890 Size: 1012 4818 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5830 BstBI TT'CG_AA Cuts at: 2812 4634 2812 Size: 1822 4008 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5830 BstXI CCAn_nnnn'nTGG Cuts at: 2128 2332 2128 Size: 204 5626 BstYI r'GATC_y Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825 Size: 1188 367 385 17 1452 86 12 346 Cuts at: 5825 1972 Size: 1977 Fragments arranged by size: 1977 1452 1188 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2928 513 Size: 552 939 924 3415 Fragments arranged by size: 3415 939 924 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5091 Fragments arranged by size: 5091 683 56 BtsI GCAGTG_nn' Cuts at: 2615 2920 2928 3127 3346 4140 4160 2615 Size: 305 8 199 219 794 20 4285 Fragments arranged by size: 4285 794 305 219 199 20 8 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4837 809 Size: 507 3521 1802 Fragments arranged by size: 3521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2292 2778 3277 Size: 54 1425 50 275 18 462 486 499 Cuts at: 3277 4166 5795 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 499 486 462 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3760 1353 Size: 2407 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4417 4791 4937 387 Size: 1064 138 2828 374 146 1280 Fragments arranged by size: 2828 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2262 3893 4490 5277 5545 1239 Size: 393 455 175 1631 597 787 268 1524 Fragments arranged by size: 1631 1524 787 597 455 393 268 175 EcoRI G'AATT_C Cuts at: 2566 3139 3517 2566 Size: 573 378 4879 Fragments arranged by size: 4879 573 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2113 2113 Size: 5830 FspI TGC'GCA Cuts at: 2027 2621 4336 2027 Size: 594 1715 3521 Fragments arranged by size: 3521 1715 594 HaeII r_GCGC'y Cuts at: 1678 2643 4977 1678 Size: 965 2334 2531 Fragments arranged by size: 2531 2334 965 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2380 2412 4471 4503 4545 Size: 32 135 32 808 32 2059 32 42 Cuts at: 4545 4577 1373 Size: 32 2626 Fragments arranged by size: 2626 2059 808 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3174 3420 669 Size: 988 281 1236 246 3079 Fragments arranged by size: 3079 1236 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KasI G'GCGC_C Cuts at: 2639 2639 Size: 5830 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4944 5128 1305 Size: 3639 184 2007 Fragments arranged by size: 3639 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2780 10 Size: 54 1425 343 948 3060 Fragments arranged by size: 3060 1425 948 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2489 2555 2786 2943 3009 3788 4147 Size: 648 1323 66 231 157 66 779 359 Cuts at: 4147 4306 518 Size: 159 2042 Fragments arranged by size: 2042 1323 779 648 359 231 159 157 66 66 MspA1I CmG'CkG Cuts at: 1606 2150 2472 2481 2650 3246 3923 5071 5316 Size: 544 322 9 169 596 677 1148 245 Cuts at: 5316 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 596 544 322 245 169 9 NaeI GCC'GGC Cuts at: 2692 2692 Size: 5830 NarI GG'CG_CC Cuts at: 2640 2640 Size: 5830 NcoI C'CATG_G Cuts at: 513 1065 2004 2928 513 Size: 552 939 924 3415 Fragments arranged by size: 3415 939 924 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NgoMIV G'CCGG_C Cuts at: 2690 2690 Size: 5830 NheI G'CTAG_C Cuts at: 1052 2038 1052 Size: 986 4844 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 3188 4733 1132 Size: 97 1959 1545 2229 Fragments arranged by size: 2229 1959 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5830 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5830 PfoI T'CCnGG_A Cuts at: 2331 2364 2835 3044 2331 Size: 33 471 209 5117 Fragments arranged by size: 5117 471 209 33 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PspXI vC'TCGA_Gb Cuts at: 2784 2784 Size: 5830 PstI C_TGCA'G Cuts at: 830 3182 830 Size: 2352 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 3178 4313 4994 5185 826 Size: 219 2133 1135 681 191 1471 Fragments arranged by size: 2133 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2886 2886 Size: 5830 SfoI GGC'GCC Cuts at: 2641 2641 Size: 5830 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 3157 1872 Size: 1285 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2784 3934 4835 5097 820 Size: 197 926 841 1150 901 262 1553 Fragments arranged by size: 1553 1150 926 901 841 262 197 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5830 StyI C'CwwG_G Cuts at: 513 1065 2004 2457 2793 2908 2928 3269 513 Size: 552 939 453 336 115 20 341 3074 Fragments arranged by size: 3074 939 552 453 341 336 115 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2338 3004 3857 3874 4027 4212 4657 Size: 1255 758 666 853 17 153 185 445 Cuts at: 4657 325 Size: 1498 Fragments arranged by size: 1498 1255 853 758 666 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4076 5788 96 Size: 274 80 33 51 1443 2099 1712 138 Fragments arranged by size: 2099 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2313 2345 2699 2731 5790 5822 2313 Size: 32 354 32 3059 32 2321 Fragments arranged by size: 3059 2321 354 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2521 2011 Size: 510 5320 XhoI C'TCGA_G Cuts at: 1943 2784 1943 Size: 841 4989 XmaI C'CCGG_G Cuts at: 1870 3155 1870 Size: 1285 4545 XmnI GAAnn'nnTTC Cuts at: 3959 3959 Size: 5830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SrfI SwaI