Restriction map for
pFN21AB4959
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI ApaI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BalI BamHI BbeI BbvCI
BclI BfrI BlnI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
Bst1107I BstEII BstPI BstXI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV FbaI
FseI FspI HindIII HpaI HphI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NarI
NcoI NdeI NheI NotI PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AatII 1514 GACGTC GACGT^C
AccI 1372 GTCTAC GT^[AC][GT]AC
AcsI 326 AAATTC [GA]^AATT[CT]
AocI 639 CCTGAGG CC^T[ACGT]AGG
Aor51HI 704 AGCGCT AGC^GCT
ApoI 326 AAATTC [GA]^AATT[CT]
AspI 1308 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 206 GGCACC G^G[CT][GA]CC
BanII 243 GAGCTC G[GA]GC[CT]^C
BbrPI 493 CACGTG CAC^GTG
BciVI 1680 GGATAC GTATCC(6/5)
BcnI 516 CCGGG CC^[GC]GG
BglII 341 AGATCT A^GATCT
BsaAI 493 CACGTG [CT]AC^GT[GA]
BsaBI 1581 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 642 GAGGAG GAGGAG(10/8)
BsmI 1531 GCATTC GAATGC(1/-1)
Bsp1407I 262 TGTACA T^GTACA
BsrFI 1157 GCCGGC [GA]^CCGG[CT]
BsrGI 262 TGTACA T^GTACA
BssHII 32 GCGCGC G^CGCGC
BssSI 751 CACGAG CACGAG(-5/-1)
BstBI 926 TTCGAA TT^CGAA
Bsu36I 639 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1157 GCCGGC [GA]^CCGG[CT]
DraI 408 TTTAAA TTT^AAA
DraIII 169 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1404 GACGCACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 137 CCGTGG C^C[GA][CT]GG
EaeI 1462 TGGCCG [CT]^GGCC[GA]
Eco47III 704 AGCGCT AGC^GCT
Eco81I 639 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1459 CCTTGG C^C[AT][AT]GG
EcoT22I 1529 ATGCAT ATGCA^T
HaeII 704 AGCGCT [GA]GCGC^[CT]
HincII 778 GTCAAC GT[CT]^[GA]AC
HindII 778 GTCAAC GT[CT]^[GA]AC
MamI 1581 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1488 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1150 CAGCTG C[AC]G^C[GT]G
NaeI 1157 GCCGGC GCC^GGC
NciI 516 CCGGG CC^[GC]GG
NgoMIV 1157 GCCGGC G^CCGGC
NruI 1266 TCGCGA TCG^CGA
NsiI 1529 ATGCAT ATGCA^T
NspI 526 ACATGC [GA]CATG^[CT]
NspV 926 TTCGAA TT^CGAA
PflFI 1308 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1218 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 493 CACGTG CAC^GTG
PmlI 493 CACGTG CAC^GTG
PvuII 1150 CAGCTG CAG^CTG
SacI 243 GAGCTC GAGCT^C
SexAI 170 ACCTGGT A^CC[AT]GGT
SfiI 747 GGCCCACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 926 TTCGAA TT^CGAA
SmlI 471 CTTGAG C^T[CT][GA]AG
SspBI 262 TGTACA T^GTACA
StuI 1134 AGGCCT AGG^CCT
StyI 1459 CCTTGG C^C[AT][AT]GG
Tth111I 1308 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1218 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1626 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 84 GACGCC G[GA]^CG[CT]C
1514 GACGTC
AfaI 150 GTAC GT^AC
263 GTAC
Alw44I 656 GTGCAC G^TGCAC
1180 GTGCAC
ApaLI 656 GTGCAC G^TGCAC
1180 GTGCAC
Asp700I 430 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
919 GAAATGCTTC
BfaI 1195 CTAG C^TAG
1627 CTAG
BglI 748 GCCCACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1014 GCCCTTCCGGC
BlpI 240 GCTGAGC GC^T[ACGT]AGC
1152 GCTGAGC
Bpu1102I 240 GCTGAGC GC^T[ACGT]AGC
1152 GCTGAGC
BsaHI 84 GACGCC G[GA]^CG[CT]C
1514 GACGTC
BsaI 488 GAGACC GGTCTC(1/5)
1228 GAGACC
BstAPI 378 GCAGTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
530 GCAGCCACTGC
BstF5I 1188 CATCC GGATG(2/0)
1391 GGATG
CelII 240 GCTGAGC GC^T[ACGT]AGC
1152 GCTGAGC
DraII 1220 AGGTCCT [GA]G^G[ACGT]CC[CT]
1504 GGGTCCT
EcoNI 100 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
783 CCTGCTGAAGG
EcoO109I 1220 AGGTCCT [GA]G^G[ACGT]CC[CT]
1504 GGGTCCT
FauI 48 CCCGC CCCGC(4/6)
1111 GCGGG
FokI 1188 CATCC GGATG(9/13)
1391 GGATG
Hin1I 84 GACGCC G[GA]^CG[CT]C
1514 GACGTC
MaeI 1195 CTAG C^TAG
1627 CTAG
PpuMI 1220 AGGTCCT [GA]G^G[AT]CC[CT]
1504 GGGTCCT
PstI 37 CTGCAG CTGCA^G
565 CTGCAG
RsaI 150 GTAC GT^AC
263 GTAC
TfiI 937 GAATC G^A[AT]TC
1263 GATTC
Tsp45I 1081 GTGAC ^GT[GC]AC
1480 GTGAC
XmnI 430 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
919 GAAATGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BbsI BbvI BcgI
BmyI BpmI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII