(Circular) (Six-base) MAPSORT of: pFN21AB4959.seq Check: 3875 from: 1 to: 6409 pFN21AB4959 6409 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3523 4219 278 Size: 53 83 186 936 1987 696 2468 Fragments arranged by size: 2468 1987 936 696 186 83 53 AccI GT'mk_AC Cuts at: 1937 3378 3752 1937 Size: 1441 374 4594 Fragments arranged by size: 4594 1441 374 Acc65I G'GTAC_C Cuts at: 3730 3730 Size: 6409 AclI AA'CG_TT Cuts at: 1563 4536 4909 1563 Size: 2973 373 3063 Fragments arranged by size: 3063 2973 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 2813 3014 4472 5856 6124 1632 Size: 695 486 201 1458 1384 268 1917 Fragments arranged by size: 1917 1458 1384 695 486 268 201 AfeI AGC'GCT Cuts at: 2711 2711 Size: 6409 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6212 AflIII A'CryG_T Cuts at: 1203 5308 6285 1203 Size: 4105 977 1327 Fragments arranged by size: 4105 1327 977 AgeI A'CCGG_T Cuts at: 5240 5240 Size: 6409 AhdI GACnn_n'nnGTC Cuts at: 5138 5138 Size: 6409 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6409 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6409 AlwNI CAG_nnn'CTG Cuts at: 2541 2697 3178 3229 4152 5724 2541 Size: 156 481 51 923 1572 3226 Fragments arranged by size: 3226 1572 923 481 156 51 ApaLI G'TGCA_C Cuts at: 2661 3185 4466 5622 2661 Size: 524 1281 1156 3448 Fragments arranged by size: 3448 1281 1156 524 ApoI r'AATT_y Cuts at: 1493 2331 3718 3944 4096 6205 1493 Size: 838 1387 226 152 2109 1697 Fragments arranged by size: 2109 1697 1387 838 226 152 AseI AT'TA_AT Cuts at: 160 1032 4963 160 Size: 872 3931 1606 Fragments arranged by size: 3931 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6409 AvaI C'yCGr_G Cuts at: 1870 1943 3734 1870 Size: 73 1791 4545 Fragments arranged by size: 4545 1791 73 BamHI G'GATC_C Cuts at: 3739 4106 3739 Size: 367 6042 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2211 3730 5185 618 Size: 325 217 627 424 1519 1455 1842 Fragments arranged by size: 1842 1519 1455 627 424 325 217 BanII G_rGCy'C Cuts at: 1391 2252 3728 1391 Size: 861 1476 4072 Fragments arranged by size: 4072 1476 861 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2144 2589 3317 928 Size: 971 245 445 728 4020 Fragments arranged by size: 4020 971 728 445 245 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2642 2676 2794 2828 3110 3144 4600 Size: 34 705 34 118 34 282 34 1456 Cuts at: 4600 4634 1903 Size: 34 3678 Fragments arranged by size: 3678 1456 705 282 118 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3679 4301 5511 3679 Size: 622 1210 4577 Fragments arranged by size: 4577 1210 622 BclI T'GATC_A Cuts at: 1548 1548 Size: 6409 BfrBI ATG'CAT Cuts at: 3536 3536 Size: 6409 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2759 3025 5020 Size: 107 122 71 631 605 1087 266 1995 Cuts at: 5020 136 Size: 1525 Fragments arranged by size: 1995 1525 1087 631 605 266 122 107 71 BglII A'GATC_T Cuts at: 2346 6404 2346 Size: 4058 2351 BlpI GC'TnA_GC Cuts at: 2246 3158 3826 2246 Size: 912 668 4829 Fragments arranged by size: 4829 912 668 Bme1580I G_kGCm'C Cuts at: 1790 2214 2665 3189 4470 5626 1790 Size: 424 451 524 1281 1156 2573 Fragments arranged by size: 2573 1281 1156 524 451 424 BmgBI CAC'GTC Cuts at: 1318 2782 1318 Size: 1464 4945 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3043 3602 4140 Size: 416 53 83 399 24 1626 559 538 Cuts at: 4140 5098 442 Size: 958 1753 Fragments arranged by size: 1753 1626 958 559 538 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6409 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2782 2782 Size: 6409 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3059 3116 3167 3251 3600 5069 1239 Size: 1820 57 51 84 349 1469 2579 Fragments arranged by size: 2579 1820 1469 349 84 57 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2497 4534 5399 5697 2497 Size: 2037 865 298 3209 Fragments arranged by size: 3209 2037 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2487 3227 5072 882 Size: 617 988 740 1845 2219 Fragments arranged by size: 2219 1845 988 740 617 BsaAI yAC'GTr Cuts at: 493 1204 2500 4200 493 Size: 711 1296 1700 2702 Fragments arranged by size: 2702 1700 1296 711 BsaBI GATnn'nnATC Cuts at: 3590 6403 3590 Size: 2813 3596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2090 3520 4216 4598 Size: 53 83 186 936 557 1430 696 382 Cuts at: 4598 5233 275 Size: 635 1451 Fragments arranged by size: 1451 1430 936 696 635 557 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4842 5240 5514 5661 6072 4842 Size: 398 274 147 411 5179 Fragments arranged by size: 5179 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2138 2168 2495 2525 1178 Size: 30 165 30 735 30 327 30 5062 Fragments arranged by size: 5062 735 327 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2662 1186 Size: 1476 4933 BseYI C'CCAG_C Cuts at: 2084 2392 2690 3153 5612 6108 2084 Size: 308 298 463 2459 496 2385 Fragments arranged by size: 2459 2385 496 463 308 298 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 2206 2397 2647 2795 1163 Size: 573 315 155 191 250 148 4777 Fragments arranged by size: 4777 573 315 250 191 155 148 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3859 4620 5648 1540 Size: 275 186 1858 761 1028 2301 Fragments arranged by size: 2301 1858 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2252 2665 3189 3258 4470 4555 5626 2252 Size: 413 524 69 1212 85 1071 3035 Fragments arranged by size: 3035 1212 1071 524 413 85 69 BsmI GAATG_Cn' Cuts at: 3536 3920 4013 3536 Size: 384 93 5932 Fragments arranged by size: 5932 384 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 2252 2665 3189 3258 3728 4470 Size: 399 424 38 413 524 69 470 742 Cuts at: 4470 4555 5626 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 524 470 424 413 399 85 69 38 BspEI T'CCGG_A Cuts at: 6072 6072 Size: 6409 BspHI T'CATG_A Cuts at: 4298 6028 4298 Size: 1730 4679 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2496 2796 3222 3764 844 Size: 1652 300 426 542 3489 Fragments arranged by size: 3489 1652 542 426 300 BsrBI CCG'CTC Cuts at: 1394 1823 4296 1394 Size: 429 2473 3507 Fragments arranged by size: 3507 2473 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4904 5078 65 Size: 1183 3656 174 1396 Fragments arranged by size: 3656 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3162 5053 5240 1700 Size: 110 1352 1891 187 2869 Fragments arranged by size: 2869 1891 1352 187 110 BsrGI T'GTAC_A Cuts at: 96 2267 96 Size: 2171 4238 BssHII G'CGCG_C Cuts at: 1925 2037 1925 Size: 112 6297 BssSI C'ACGA_G Cuts at: 2756 4469 5481 2756 Size: 1713 1012 3684 Fragments arranged by size: 3684 1713 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2389 2541 794 Size: 1595 152 4662 Fragments arranged by size: 4662 1595 152 BstBI TT'CG_AA Cuts at: 2932 5213 2932 Size: 2281 4128 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6409 BstYI r'GATC_y Cuts at: 1972 2346 3012 3694 3739 4106 4491 4508 5960 Size: 374 666 682 45 367 385 17 1452 Cuts at: 5960 6046 6058 6404 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 682 666 385 374 367 346 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 2645 2645 Size: 6409 BtgI C'CryG_G Cuts at: 513 1065 2004 2142 513 Size: 552 939 138 4780 Fragments arranged by size: 4780 939 552 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5670 Fragments arranged by size: 5670 683 56 BtsI GCAGTG_nn' Cuts at: 2086 2539 3925 4719 4739 2086 Size: 453 1386 794 20 3756 Fragments arranged by size: 3756 1386 794 453 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6409 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6374 DraI TTT'AAA Cuts at: 2415 3713 2415 Size: 1298 5111 DraIII CAC_nnn'GTG Cuts at: 2179 2179 Size: 6409 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3415 5416 809 Size: 507 2099 2001 1802 Fragments arranged by size: 2099 2001 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3467 3856 4745 Size: 54 1425 50 275 18 1637 389 889 Cuts at: 4745 6374 8 Size: 1629 43 Fragments arranged by size: 1637 1629 1425 889 389 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3856 1537 Size: 275 2044 4090 Fragments arranged by size: 4090 2044 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2257 2346 3050 4339 1353 Size: 904 89 704 1289 3423 Fragments arranged by size: 3423 1289 904 704 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2758 3185 3301 4996 5370 5516 Size: 1064 138 1169 427 116 1695 374 146 Cuts at: 5516 387 Size: 1280 Fragments arranged by size: 1695 1280 1169 1064 427 374 146 138 116 EcoICRI GAG'CTC Cuts at: 2250 2250 Size: 6409 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2813 3014 3059 3116 3167 3251 Size: 393 695 486 201 45 57 51 84 Cuts at: 3251 3600 4472 5069 5856 6124 1239 Size: 349 872 597 787 268 1524 Fragments arranged by size: 1524 872 787 695 597 486 393 349 268 201 84 57 51 45 EcoNI CCTnn'n_nnAGG Cuts at: 2109 2792 2109 Size: 683 5726 EcoO109I rG'GnC_Cy Cuts at: 3226 3510 3226 Size: 284 6125 EcoRI G'AATT_C Cuts at: 3718 4096 3718 Size: 378 6031 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2845 2845 Size: 6409 FspI TGC'GCA Cuts at: 4915 4915 Size: 6409 HaeII r_GCGC'y Cuts at: 1678 2713 5556 1678 Size: 1035 2843 2531 Fragments arranged by size: 2843 2531 1035 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2014 2046 2338 2370 3243 Size: 32 135 32 442 32 292 32 873 Cuts at: 3243 3275 3494 3526 5050 5082 5124 5156 1373 Size: 32 219 32 1524 32 42 32 2626 Fragments arranged by size: 2626 1524 873 442 292 219 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2785 3753 3999 669 Size: 988 281 847 968 246 3079 Fragments arranged by size: 3079 988 968 847 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6409 HpaI GTT'AAC Cuts at: 3999 3999 Size: 6409 KpnI G_GTAC'C Cuts at: 3734 3734 Size: 6409 MfeI C'AATT_G Cuts at: 4008 4008 Size: 6409 MluI A'CGCG_T Cuts at: 6285 6285 Size: 6409 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2336 5523 5707 1305 Size: 1031 3187 184 2007 Fragments arranged by size: 3187 2007 1031 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4587 Fragments arranged by size: 4587 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3497 4367 4726 4885 518 Size: 648 2331 870 359 159 2042 Fragments arranged by size: 2331 2042 870 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3157 3825 4502 5650 5895 1606 Size: 1551 668 677 1148 245 2120 Fragments arranged by size: 2120 1551 1148 677 668 245 NaeI GCC'GGC Cuts at: 3164 3164 Size: 6409 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4918 Fragments arranged by size: 4918 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6409 NgoMIV G'CCGG_C Cuts at: 3162 3162 Size: 6409 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6409 NotI GC'GGCC_GC Cuts at: 3856 3856 Size: 6409 NruI TCG'CGA Cuts at: 3273 3273 Size: 6409 NsiI A_TGCA'T Cuts at: 3538 3538 Size: 6409 NspI r_CATG'y Cuts at: 1132 1229 2535 3767 5312 1132 Size: 97 1306 1232 1545 2229 Fragments arranged by size: 2229 1545 1306 1232 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6409 PciI A'CATG_T Cuts at: 5308 5308 Size: 6409 PflMI CCAn_nnn'nTGG Cuts at: 1108 3229 1108 Size: 2121 4288 PfoI T'CCnGG_A Cuts at: 3228 3228 Size: 6409 PmeI GTTT'AAAC Cuts at: 3713 3713 Size: 6409 PmlI CAC'GTG Cuts at: 2500 2500 Size: 6409 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4478 4510 5991 6023 4478 Size: 32 1481 32 4864 Fragments arranged by size: 4864 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3226 3510 3226 Size: 284 6125 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6409 PsiI TTA'TAA Cuts at: 3979 3979 Size: 6409 PstI C_TGCA'G Cuts at: 830 2046 2574 3761 830 Size: 1216 528 1187 3478 Fragments arranged by size: 3478 1216 1187 528 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6409 PvuII CAG'CTG Cuts at: 3157 3157 Size: 6409 SacI G_AGCT'C Cuts at: 2252 2252 Size: 6409 SalI G'TCGA_C Cuts at: 1936 3751 1936 Size: 1815 4594 SapI GCTCTTCn'nnn_ Cuts at: 2257 2346 3050 2257 Size: 89 704 5616 Fragments arranged by size: 5616 704 89 SbfI CC_TGCA'GG Cuts at: 3761 3761 Size: 6409 ScaI AGT'ACT Cuts at: 4657 6369 4657 Size: 1712 4697 SexAI A'CCwGG_T Cuts at: 2175 2175 Size: 6409 SfcI C'TryA_G Cuts at: 826 1045 2042 2570 2698 3757 4892 5573 5764 Size: 219 997 528 128 1059 1135 681 191 Cuts at: 5764 826 Size: 1471 Fragments arranged by size: 1471 1135 1059 997 681 528 219 191 128 SfiI GGCCn_nnn'nGGCC Cuts at: 2759 2759 Size: 6409 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6409 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6409 SmaI CCC'GGG Cuts at: 1872 3736 1872 Size: 1864 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2476 4513 5414 5676 820 Size: 197 926 533 2037 901 262 1553 Fragments arranged by size: 2037 1553 926 901 533 262 197 SnaBI TAC'GTA Cuts at: 493 4200 493 Size: 3707 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6409 SphI G_CATG'C Cuts at: 1132 3767 1132 Size: 2635 3774 SspI AAT'ATT Cuts at: 5 52 4333 5 Size: 47 4281 2081 Fragments arranged by size: 4281 2081 47 StuI AGG'CCT Cuts at: 1517 3141 1517 Size: 1624 4785 StyI C'CwwG_G Cuts at: 513 1065 2004 3464 3848 513 Size: 552 939 1460 384 3074 Fragments arranged by size: 3074 1460 939 552 384 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2216 3104 4436 4453 4606 4791 5236 Size: 1255 636 888 1332 17 153 185 445 Cuts at: 5236 325 Size: 1498 Fragments arranged by size: 1498 1332 1255 888 636 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2154 2267 4655 Size: 274 80 33 51 1443 177 113 2388 Cuts at: 4655 6367 96 Size: 1712 138 Fragments arranged by size: 2388 1712 1443 274 177 138 113 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2495 2527 2578 2610 6369 6401 2495 Size: 32 51 32 3759 32 2503 Fragments arranged by size: 3759 2503 51 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3316 1316 Size: 2000 4409 XbaI T'CTAG_A Cuts at: 3631 3745 3631 Size: 114 6295 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6409 XmaI C'CCGG_G Cuts at: 1870 3734 1870 Size: 1864 4545 XmnI GAAnn'nnTTC Cuts at: 2439 2928 4538 2439 Size: 489 1610 4310 Fragments arranged by size: 4310 1610 489 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3521 4217 276 Size: 53 83 186 936 1987 696 2468 Fragments arranged by size: 2468 1987 936 696 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NarI PacI PspOMI PspXI PsrI RsrII SacII SanDI SfoI SrfI SwaI XcmI