(Circular) (Six-base) MAPSORT of: pFN21AB4568.seq Check: 624 from: 1 to: 5815 pFN21AB4568 5815 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3625 278 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3158 1937 Size: 1221 4594 Acc65I G'GTAC_C Cuts at: 3136 3136 Size: 5815 AclI AA'CG_TT Cuts at: 1563 2422 3942 4315 1563 Size: 859 1520 373 3063 Fragments arranged by size: 3063 1520 859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3878 5262 5530 1632 Size: 2246 1384 268 1917 Fragments arranged by size: 2246 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5618 AflIII A'CryG_T Cuts at: 1203 4714 5691 1203 Size: 3511 977 1327 Fragments arranged by size: 3511 1327 977 AgeI A'CCGG_T Cuts at: 4646 4646 Size: 5815 AhdI GACnn_n'nnGTC Cuts at: 4544 4544 Size: 5815 AleI CACnn'nnGTG Cuts at: 1166 2522 1166 Size: 1356 4459 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5815 AlwNI CAG_nnn'CTG Cuts at: 3558 5130 3558 Size: 1572 4243 ApaLI G'TGCA_C Cuts at: 3872 5028 3872 Size: 1156 4659 ApoI r'AATT_y Cuts at: 1493 2783 3124 3350 3502 5611 1493 Size: 1290 341 226 152 2109 1697 Fragments arranged by size: 2109 1697 1290 341 226 152 AseI AT'TA_AT Cuts at: 160 1032 2301 4369 160 Size: 872 1269 2068 1606 Fragments arranged by size: 2068 1606 1269 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 AvaI C'yCGr_G Cuts at: 1870 1943 3140 1870 Size: 73 1197 4545 Fragments arranged by size: 4545 1197 73 BamHI G'GATC_C Cuts at: 2271 3145 3512 2271 Size: 874 367 4574 Fragments arranged by size: 4574 874 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2190 2466 2480 3136 4591 Size: 325 217 627 403 276 14 656 1455 Cuts at: 4591 618 Size: 1842 Fragments arranged by size: 1842 1455 656 627 403 325 276 217 14 BanII G_rGCy'C Cuts at: 1391 2162 2348 3134 1391 Size: 771 186 786 4072 Fragments arranged by size: 4072 786 771 186 BbeI G_GCGC'C Cuts at: 2194 2470 2194 Size: 276 5539 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2242 928 Size: 971 343 4501 Fragments arranged by size: 4501 971 343 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4006 4040 1903 Size: 34 50 34 1985 34 3678 Fragments arranged by size: 3678 1985 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3707 4917 3707 Size: 1210 4605 BclI T'GATC_A Cuts at: 1548 1548 Size: 5815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 2211 2231 Size: 107 122 71 631 605 364 175 20 Cuts at: 2231 4426 136 Size: 2195 1525 Fragments arranged by size: 2195 1525 631 605 364 175 122 107 71 20 BglII A'GATC_T Cuts at: 2294 5810 2294 Size: 3516 2299 BlpI GC'TnA_GC Cuts at: 3018 3232 3018 Size: 214 5601 Bme1580I G_kGCm'C Cuts at: 1790 3876 5032 1790 Size: 2086 1156 2573 Fragments arranged by size: 2573 2086 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5815 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2656 3546 4504 Size: 416 53 83 399 24 1239 890 958 Cuts at: 4504 442 Size: 1753 Fragments arranged by size: 1753 1239 958 890 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2352 2370 2621 2763 2969 4475 1239 Size: 1113 18 251 142 206 1506 2579 Fragments arranged by size: 2579 1506 1113 251 206 142 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3940 4805 5103 3940 Size: 865 298 4652 Fragments arranged by size: 4652 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4478 882 Size: 617 2979 2219 Fragments arranged by size: 2979 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3606 493 Size: 711 2402 2702 Fragments arranged by size: 2702 2402 711 BsaBI GATnn'nnATC Cuts at: 5809 5809 Size: 5815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2191 2247 2467 3622 Size: 53 83 186 936 658 56 220 1155 Cuts at: 3622 4004 4639 275 Size: 382 635 1451 Fragments arranged by size: 1451 1155 936 658 635 382 220 186 83 56 53 BsaWI w'CCGG_w Cuts at: 4248 4646 4920 5067 5478 4248 Size: 398 274 147 411 4585 Fragments arranged by size: 4585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 1178 Size: 30 165 30 867 30 4693 Fragments arranged by size: 4693 867 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2279 2294 2297 1186 Size: 979 114 15 3 4704 Fragments arranged by size: 4704 979 114 15 3 BseYI C'CCAG_C Cuts at: 5018 5514 5018 Size: 496 5319 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2183 2942 1163 Size: 573 447 759 4036 Fragments arranged by size: 4036 759 573 447 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2143 2182 2206 3265 4026 5054 Size: 275 186 142 39 24 1059 761 1028 Cuts at: 5054 1540 Size: 2301 Fragments arranged by size: 2301 1059 1028 761 275 186 142 39 24 BsiHKAI G_wGCw'C Cuts at: 2162 2348 3876 3961 5032 2162 Size: 186 1528 85 1071 2945 Fragments arranged by size: 2945 1528 1071 186 85 BsmI GAATG_Cn' Cuts at: 3326 3419 3326 Size: 93 5722 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 2348 3134 3876 3961 5032 1391 Size: 399 372 186 786 742 85 1071 2174 Fragments arranged by size: 2174 1071 786 742 399 372 186 85 BspEI T'CCGG_A Cuts at: 5478 5478 Size: 5815 BspHI T'CATG_A Cuts at: 3704 5434 3704 Size: 1730 4085 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3170 844 Size: 2326 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3702 1394 Size: 429 1879 3507 Fragments arranged by size: 3507 1879 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4310 4484 65 Size: 1183 3062 174 1396 Fragments arranged by size: 3062 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2193 2243 4459 4646 1700 Size: 110 383 50 2216 187 2869 Fragments arranged by size: 2869 2216 383 187 110 50 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5815 BssHII G'CGCG_C Cuts at: 1925 2113 2115 1925 Size: 188 2 5625 Fragments arranged by size: 5625 188 2 BssSI C'ACGA_G Cuts at: 3875 4887 3875 Size: 1012 4803 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5815 BstBI TT'CG_AA Cuts at: 4619 4619 Size: 5815 BstEII G'GTnAC_C Cuts at: 1184 2650 1184 Size: 1466 4349 BstXI CCAn_nnnn'nTGG Cuts at: 2986 2986 Size: 5815 BstYI r'GATC_y Cuts at: 1972 2271 2294 3145 3512 3897 3914 5366 5452 Size: 299 23 851 367 385 17 1452 86 Cuts at: 5452 5464 5810 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 851 385 367 346 299 86 23 17 12 BtgI C'CryG_G Cuts at: 513 1065 2012 513 Size: 552 947 4316 Fragments arranged by size: 4316 947 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5076 Fragments arranged by size: 5076 683 56 BtsI GCAGTG_nn' Cuts at: 3331 4125 4145 3331 Size: 794 20 5001 Fragments arranged by size: 5001 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5780 DraI TTT'AAA Cuts at: 3119 3119 Size: 5815 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4822 809 Size: 507 3506 1802 Fragments arranged by size: 3506 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2073 2140 Size: 54 1425 50 275 18 195 48 67 Cuts at: 2140 2179 2203 2212 2964 3262 4151 5780 8 Size: 39 24 9 752 298 889 1629 43 Fragments arranged by size: 1629 1425 889 752 298 275 195 67 54 50 48 43 39 24 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2140 2179 2203 3262 1537 Size: 275 328 39 24 1059 4090 Fragments arranged by size: 4090 1059 328 275 39 24 EarI CTCTTCn'nnn_ Cuts at: 1353 3745 1353 Size: 2392 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4402 4776 4922 387 Size: 1064 138 2813 374 146 1280 Fragments arranged by size: 2813 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2160 2346 2160 Size: 186 5629 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2352 2370 2621 2763 2969 3878 4475 Size: 393 720 18 251 142 206 909 597 Cuts at: 4475 5262 5530 1239 Size: 787 268 1524 Fragments arranged by size: 1524 909 787 720 597 393 268 251 206 142 18 EcoO109I rG'GnC_Cy Cuts at: 2056 2056 Size: 5815 EcoRI G'AATT_C Cuts at: 2783 3124 3502 2783 Size: 341 378 5096 Fragments arranged by size: 5096 378 341 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2467 2467 Size: 5815 FspI TGC'GCA Cuts at: 4321 4321 Size: 5815 HaeII r_GCGC'y Cuts at: 1678 2069 2194 2470 4962 1678 Size: 391 125 276 2492 2531 Fragments arranged by size: 2531 2492 391 276 125 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2996 3028 4456 4488 4530 Size: 32 135 32 1424 32 1428 32 42 Cuts at: 4530 4562 1373 Size: 32 2626 Fragments arranged by size: 2626 1428 1424 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3159 3405 669 Size: 988 281 1221 246 3079 Fragments arranged by size: 3079 1221 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5815 HpaI GTT'AAC Cuts at: 3405 3405 Size: 5815 KasI G'GCGC_C Cuts at: 2190 2466 2190 Size: 276 5539 KpnI G_GTAC'C Cuts at: 3140 3140 Size: 5815 MfeI C'AATT_G Cuts at: 3414 3414 Size: 5815 MluI A'CGCG_T Cuts at: 5691 5691 Size: 5815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 2897 4929 5113 1305 Size: 993 599 2032 184 2007 Fragments arranged by size: 2032 2007 993 599 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 2214 2966 10 Size: 54 1425 343 243 139 752 2859 Fragments arranged by size: 2859 1425 752 343 243 139 54 MslI CAynn'nnrTG Cuts at: 518 1166 2522 2732 2763 3773 4132 4291 518 Size: 648 1356 210 31 1010 359 159 2042 Fragments arranged by size: 2042 1356 1010 648 359 210 159 31 MspA1I CmG'CkG Cuts at: 1606 2014 2554 3087 3231 3908 5056 5301 1606 Size: 408 540 533 144 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 540 533 408 245 144 NaeI GCC'GGC Cuts at: 2195 2245 2195 Size: 50 5765 NarI GG'CG_CC Cuts at: 2191 2467 2191 Size: 276 5539 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5263 NdeI CA'TA_TG Cuts at: 387 2803 387 Size: 2416 3399 NgoMIV G'CCGG_C Cuts at: 2193 2243 2193 Size: 50 5765 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5815 NotI GC'GGCC_GC Cuts at: 3262 3262 Size: 5815 NspI r_CATG'y Cuts at: 1132 1229 2938 3173 4718 1132 Size: 97 1709 235 1545 2229 Fragments arranged by size: 2229 1709 1545 235 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5815 PciI A'CATG_T Cuts at: 4714 4714 Size: 5815 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5815 PfoI T'CCnGG_A Cuts at: 2274 2946 2274 Size: 672 5143 PmeI GTTT'AAAC Cuts at: 3119 3119 Size: 5815 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3884 3916 5397 5429 3884 Size: 32 1481 32 4270 Fragments arranged by size: 4270 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5815 PsiI TTA'TAA Cuts at: 3385 3385 Size: 5815 PstI C_TGCA'G Cuts at: 830 3167 830 Size: 2337 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5815 PvuII CAG'CTG Cuts at: 2554 3087 2554 Size: 533 5282 SacI G_AGCT'C Cuts at: 2162 2348 2162 Size: 186 5629 SacII CC_GC'GG Cuts at: 2015 2015 Size: 5815 SalI G'TCGA_C Cuts at: 1936 3157 1936 Size: 1221 4594 SbfI CC_TGCA'GG Cuts at: 3167 3167 Size: 5815 ScaI AGT'ACT Cuts at: 4063 5775 4063 Size: 1712 4103 SfcI C'TryA_G Cuts at: 826 1045 3163 4298 4979 5170 826 Size: 219 2118 1135 681 191 1471 Fragments arranged by size: 2118 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2211 2211 Size: 5815 SfoI GGC'GCC Cuts at: 2192 2468 2192 Size: 276 5539 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5815 SmaI CCC'GGG Cuts at: 1872 3142 1872 Size: 1270 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3919 4820 5082 820 Size: 197 926 1976 901 262 1553 Fragments arranged by size: 1976 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3606 493 Size: 3113 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5815 SphI G_CATG'C Cuts at: 1132 2938 3173 1132 Size: 1806 235 3774 Fragments arranged by size: 3774 1806 235 SspI AAT'ATT Cuts at: 5 52 3739 5 Size: 47 3687 2081 Fragments arranged by size: 3687 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5815 StyI C'CwwG_G Cuts at: 513 1065 3045 3254 513 Size: 552 1980 209 3074 Fragments arranged by size: 3074 1980 552 209 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2498 3842 3859 4012 4197 4642 325 Size: 1255 918 1344 17 153 185 445 1498 Fragments arranged by size: 1498 1344 1255 918 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2673 4061 5773 Size: 274 80 33 51 1443 696 1388 1712 Cuts at: 5773 96 Size: 138 Fragments arranged by size: 1712 1443 1388 696 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5775 5807 5775 Size: 32 5783 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5815 XbaI T'CTAG_A Cuts at: 3151 3151 Size: 5815 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5815 XmaI C'CCGG_G Cuts at: 1870 3140 1870 Size: 1270 4545 XmnI GAAnn'nnTTC Cuts at: 3944 3944 Size: 5815 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3623 276 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI XcmI