(Circular) (Six-base) MAPSORT of: pFN21AE0824.seq Check: 4427 from: 1 to: 5509 pFN21AE0824 5509 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3319 278 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2852 1937 Size: 915 4594 Acc65I G'GTAC_C Cuts at: 2830 2830 Size: 5509 AclI AA'CG_TT Cuts at: 1563 3636 4009 1563 Size: 2073 373 3063 Fragments arranged by size: 3063 2073 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3572 4956 5224 1632 Size: 1940 1384 268 1917 Fragments arranged by size: 1940 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5312 AflIII A'CryG_T Cuts at: 1203 2085 4408 5385 1203 Size: 882 2323 977 1327 Fragments arranged by size: 2323 1327 977 882 AgeI A'CCGG_T Cuts at: 4340 4340 Size: 5509 AhdI GACnn_n'nnGTC Cuts at: 4238 4238 Size: 5509 AleI CACnn'nnGTG Cuts at: 1166 2610 1166 Size: 1444 4065 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5509 AlwNI CAG_nnn'CTG Cuts at: 3252 4824 3252 Size: 1572 3937 ApaI G_GGCC'C Cuts at: 2388 2388 Size: 5509 ApaLI G'TGCA_C Cuts at: 2296 3566 4722 2296 Size: 1270 1156 3083 Fragments arranged by size: 3083 1270 1156 ApoI r'AATT_y Cuts at: 1493 2415 2818 3044 3196 5305 1493 Size: 922 403 226 152 2109 1697 Fragments arranged by size: 2109 1697 922 403 226 152 AseI AT'TA_AT Cuts at: 160 1032 4063 160 Size: 872 3031 1606 Fragments arranged by size: 3031 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 AvaI C'yCGr_G Cuts at: 1870 1943 2388 2834 1870 Size: 73 445 446 4545 Fragments arranged by size: 4545 446 445 73 BamHI G'GATC_C Cuts at: 2839 3206 2839 Size: 367 5142 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2771 2830 4285 618 Size: 325 217 627 984 59 1455 1842 Fragments arranged by size: 1842 1455 984 627 325 217 59 BanII G_rGCy'C Cuts at: 1391 2388 2828 1391 Size: 997 440 4072 Fragments arranged by size: 4072 997 440 BbeI G_GCGC'C Cuts at: 2775 2775 Size: 5509 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4538 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3700 3734 1903 Size: 34 1763 34 3678 Fragments arranged by size: 3678 1763 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3401 4611 3401 Size: 1210 4299 BclI T'GATC_A Cuts at: 1548 1548 Size: 5509 BfrBI ATG'CAT Cuts at: 2166 2166 Size: 5509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4120 136 Size: 107 122 71 631 605 2448 1525 Fragments arranged by size: 2448 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5504 5504 Size: 5509 BlpI GC'TnA_GC Cuts at: 2926 2926 Size: 5509 Bme1580I G_kGCm'C Cuts at: 1790 2300 2388 3570 4726 1790 Size: 510 88 1182 1156 2573 Fragments arranged by size: 2573 1182 1156 510 88 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5509 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3240 4198 442 Size: 416 53 83 399 24 1823 958 1753 Fragments arranged by size: 1823 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5509 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2775 2775 Size: 5509 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2308 4169 1239 Size: 1010 59 1861 2579 Fragments arranged by size: 2579 1861 1010 59 Bpu10I CC'TnA_GC Cuts at: 2760 2760 Size: 5509 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2414 3634 4499 4797 2414 Size: 1220 865 298 3126 Fragments arranged by size: 3126 1220 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4172 882 Size: 617 2673 2219 Fragments arranged by size: 2673 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3300 493 Size: 711 2096 2702 Fragments arranged by size: 2702 2096 711 BsaBI GATnn'nnATC Cuts at: 5503 5503 Size: 5509 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2772 3316 3698 4333 Size: 53 83 186 936 1239 544 382 635 Cuts at: 4333 275 Size: 1451 Fragments arranged by size: 1451 1239 936 635 544 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3942 4340 4614 4761 5172 3942 Size: 398 274 147 411 4279 Fragments arranged by size: 4279 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5284 Fragments arranged by size: 5284 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2590 2693 1186 Size: 1404 103 4002 Fragments arranged by size: 4002 1404 103 BseYI C'CCAG_C Cuts at: 4712 5208 4712 Size: 496 5013 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4936 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2959 3720 4748 1540 Size: 275 186 958 761 1028 2301 Fragments arranged by size: 2301 1028 958 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2300 2788 3570 3655 4726 2300 Size: 488 782 85 1071 3083 Fragments arranged by size: 3083 1071 782 488 85 BsmI GAATG_Cn' Cuts at: 2761 3020 3113 2761 Size: 259 93 5157 Fragments arranged by size: 5157 259 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2300 2388 2788 2828 3570 3655 4726 Size: 399 510 88 400 40 742 85 1071 Cuts at: 4726 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 510 400 399 88 85 40 BspEI T'CCGG_A Cuts at: 5172 5172 Size: 5509 BspHI T'CATG_A Cuts at: 2280 3398 5128 2280 Size: 1118 1730 2661 Fragments arranged by size: 2661 1730 1118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2603 2864 844 Size: 1759 261 3489 Fragments arranged by size: 3489 1759 261 BsrBI CCG'CTC Cuts at: 1394 1823 3396 1394 Size: 429 1573 3507 Fragments arranged by size: 3507 1573 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4004 4178 65 Size: 1183 2756 174 1396 Fragments arranged by size: 2756 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4153 4340 1700 Size: 110 2343 187 2869 Fragments arranged by size: 2869 2343 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5509 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5509 BssSI C'ACGA_G Cuts at: 3569 4581 3569 Size: 1012 4497 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5509 BstBI TT'CG_AA Cuts at: 4313 4313 Size: 5509 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5509 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2119 2011 Size: 108 5401 BstYI r'GATC_y Cuts at: 1972 2556 2839 3206 3591 3608 5060 5146 5158 Size: 584 283 367 385 17 1452 86 12 Cuts at: 5158 5504 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 584 385 367 346 283 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2640 513 Size: 552 1575 3382 Fragments arranged by size: 3382 1575 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4770 Fragments arranged by size: 4770 683 56 BtsI GCAGTG_nn' Cuts at: 2786 3025 3819 3839 2786 Size: 239 794 20 4456 Fragments arranged by size: 4456 794 239 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5509 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5474 DraI TTT'AAA Cuts at: 2813 2813 Size: 5509 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2022 4516 809 Size: 507 706 2494 1802 Fragments arranged by size: 2494 1802 706 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2247 2956 Size: 54 1425 50 275 18 243 174 709 Cuts at: 2956 3845 5474 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 709 275 243 174 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2956 1537 Size: 275 1144 4090 Fragments arranged by size: 4090 1144 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2411 2630 3439 1353 Size: 1058 219 809 3423 Fragments arranged by size: 3423 1058 809 219 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4096 4470 4616 387 Size: 1064 138 2507 374 146 1280 Fragments arranged by size: 2507 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2308 3572 4169 4956 5224 1239 Size: 393 617 59 1264 597 787 268 1524 Fragments arranged by size: 1524 1264 787 617 597 393 268 59 EcoNI CCTnn'n_nnAGG Cuts at: 2236 2236 Size: 5509 EcoO109I rG'GnC_Cy Cuts at: 2385 2385 Size: 5509 EcoRI G'AATT_C Cuts at: 2818 3196 2818 Size: 378 5131 FspI TGC'GCA Cuts at: 4015 4015 Size: 5509 HaeII r_GCGC'y Cuts at: 1678 2775 4656 1678 Size: 1097 1881 2531 Fragments arranged by size: 2531 1881 1097 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2293 2325 2731 2763 4150 Size: 32 135 32 721 32 406 32 1387 Cuts at: 4150 4182 4224 4256 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1387 721 406 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2458 2853 3099 669 Size: 988 281 520 395 246 3079 Fragments arranged by size: 3079 988 520 395 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5509 HpaI GTT'AAC Cuts at: 2458 3099 2458 Size: 641 4868 KasI G'GCGC_C Cuts at: 2771 2771 Size: 5509 KpnI G_GTAC'C Cuts at: 2834 2834 Size: 5509 MfeI C'AATT_G Cuts at: 3108 3108 Size: 5509 MluI A'CGCG_T Cuts at: 5385 5385 Size: 5509 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2124 2479 2599 4623 4807 1305 Size: 819 355 120 2024 184 2007 Fragments arranged by size: 2024 2007 819 355 184 120 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 2249 10 Size: 54 1425 343 243 174 3270 Fragments arranged by size: 3270 1425 343 243 174 54 MslI CAynn'nnrTG Cuts at: 518 1166 2084 2610 2639 3467 3826 3985 518 Size: 648 918 526 29 828 359 159 2042 Fragments arranged by size: 2042 918 828 648 526 359 159 29 MspA1I CmG'CkG Cuts at: 1606 2925 3602 4750 4995 1606 Size: 1319 677 1148 245 2120 Fragments arranged by size: 2120 1319 1148 677 245 NarI GG'CG_CC Cuts at: 2772 2772 Size: 5509 NcoI C'CATG_G Cuts at: 513 1065 2640 513 Size: 552 1575 3382 Fragments arranged by size: 3382 1575 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5509 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5509 NotI GC'GGCC_GC Cuts at: 2956 2956 Size: 5509 NsiI A_TGCA'T Cuts at: 2168 2168 Size: 5509 NspI r_CATG'y Cuts at: 1132 1229 2089 2354 2533 2867 4412 1132 Size: 97 860 265 179 334 1545 2229 Fragments arranged by size: 2229 1545 860 334 265 179 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5509 PciI A'CATG_T Cuts at: 2085 4408 2085 Size: 2323 3186 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5509 PmeI GTTT'AAAC Cuts at: 2813 2813 Size: 5509 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3578 3610 5091 5123 3578 Size: 32 1481 32 3964 Fragments arranged by size: 3964 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5509 PsiI TTA'TAA Cuts at: 3079 3079 Size: 5509 PspOMI G'GGCC_C Cuts at: 2384 2384 Size: 5509 PstI C_TGCA'G Cuts at: 830 2793 2861 830 Size: 1963 68 3478 Fragments arranged by size: 3478 1963 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5509 SalI G'TCGA_C Cuts at: 1936 2851 1936 Size: 915 4594 SbfI CC_TGCA'GG Cuts at: 2861 2861 Size: 5509 ScaI AGT'ACT Cuts at: 3757 5469 3757 Size: 1712 3797 SfcI C'TryA_G Cuts at: 826 1045 2202 2743 2789 2857 3992 4673 4864 Size: 219 1157 541 46 68 1135 681 191 Cuts at: 4864 826 Size: 1471 Fragments arranged by size: 1471 1157 1135 681 541 219 191 68 46 SfoI GGC'GCC Cuts at: 2773 2773 Size: 5509 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5509 SmaI CCC'GGG Cuts at: 1872 2390 2836 1872 Size: 518 446 4545 Fragments arranged by size: 4545 518 446 SmlI C'TyrA_G Cuts at: 820 1017 1943 2429 3613 4514 4776 820 Size: 197 926 486 1184 901 262 1553 Fragments arranged by size: 1553 1184 926 901 486 262 197 SnaBI TAC'GTA Cuts at: 493 3300 493 Size: 2807 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5509 SphI G_CATG'C Cuts at: 1132 2867 1132 Size: 1735 3774 SspI AAT'ATT Cuts at: 5 52 3433 5 Size: 47 3381 2081 Fragments arranged by size: 3381 2081 47 StuI AGG'CCT Cuts at: 1517 2585 1517 Size: 1068 4441 StyI C'CwwG_G Cuts at: 513 1065 2640 2948 513 Size: 552 1575 308 3074 Fragments arranged by size: 3074 1575 552 308 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3536 3553 3706 3891 4336 325 Size: 1255 1956 17 153 185 445 1498 Fragments arranged by size: 1956 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2052 2268 3755 Size: 274 80 33 51 1443 75 216 1487 Cuts at: 3755 5467 96 Size: 1712 138 Fragments arranged by size: 1712 1487 1443 274 216 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5469 5501 5469 Size: 32 5477 Tth111I GACn'n_nGTC Cuts at: 1316 2800 1316 Size: 1484 4025 XbaI T'CTAG_A Cuts at: 2845 2845 Size: 5509 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5509 XmaI C'CCGG_G Cuts at: 1870 2388 2834 1870 Size: 518 446 4545 Fragments arranged by size: 4545 518 446 XmnI GAAnn'nnTTC Cuts at: 3638 3638 Size: 5509 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3317 276 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI