Restriction map for
pFN21AB0457
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AseI AsnI Asp700I
AspEI AviII AvrII BalI BamHI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI Eam1105I EarI
EcoO65I EcoRI EcoRV FseI FspI
HindIII HpaI Ksp632I MaeI MamI
MluI MluNI MroI MscI NcoI
NdeI NheI NotI NruI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 887 GGTACC G^GTACC
AocI 714 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1360 AGCGCT AGC^GCT
ApaI 214 GGGCCC GGGCC^C
AscI 26 GGCGCGCC GG^CGCGCC
Asp718I 887 GGTACC G^GTACC
AspHI 1389 GAGCAC G[AT]GC[AT]^C
AspI 850 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1292 CCCGGG C^[CT]CG[GA]G
BbrPI 544 CACGTG CAC^GTG
BglI 1042 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 544 CACGTG [CT]AC^GT[GA]
BsaI 937 GAGACC GGTCTC(1/5)
BsgI 547 GTGCAG GTGCAG(16/14)
BsiHKAI 1389 GAGCAC G[AT]GC[AT]^C
BsoBI 1292 CCCGGG C^[CT]CG[GA]G
BsrBI 195 CCGCTC CCGCTC(-3/-3)
BsrDI 822 CATTGC GCAATG(2/0)
BssHII 27 GCGCGC G^CGCGC
BstF5I 639 GGATG GGATG(2/0)
BstYI 737 AGATCC [GA]^GATC[CT]
Bsu36I 714 CCTGAGG CC^T[ACGT]AGG
DraIII 142 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 847 GACGACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1360 AGCGCT AGC^GCT
Eco81I 714 CCTGAGG CC^T[ACGT]AGG
EcoT14I 953 CCAAGG C^C[AT][AT]GG
EcoT22I 228 ATGCAT ATGCA^T
FokI 639 GGATG GGATG(9/13)
HgaI 179 GACGC GACGC(5/10)
HincII 247 GTCAAC GT[CT]^[GA]AC
HindII 247 GTCAAC GT[CT]^[GA]AC
KpnI 887 GGTACC GGTAC^C
MfeI 1417 CAATTG C^AATTG
MflI 737 AGATCC [GA]^GATC[CT]
MunI 1417 CAATTG C^AATTG
NaeI 166 GCCGGC GCC^GGC
NgoMIV 166 GCCGGC G^CCGGC
NsiI 228 ATGCAT ATGCA^T
PflFI 850 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1339 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 544 CACGTG CAC^GTG
PmlI 544 CACGTG CAC^GTG
SfiI 1041 GGCCGCGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1292 CCCGGG CCC^GGG
StyI 953 CCAAGG C^C[AT][AT]GG
Tsp509I 1418 AATT ^AATT
Tth111I 850 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1339 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 737 AGATCC [GA]^GATC[CT]
XmaCI 1292 CCCGGG C^CCGGG
XmaI 1292 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1117 GGCGCC G[GA]^CG[CT]C
1220 GGCGCC
AfaI 888 GTAC GT^AC
1164 GTAC
AvaII 801 GGACC G^G[AT]CC
1314 GGACC
BbeI 1117 GGCGCC GGCGC^C
1220 GGCGCC
BclI 923 TGATCA T^GATCA
1154 TGATCA
BsaHI 1117 GGCGCC G[GA]^CG[CT]C
1220 GGCGCC
BsiEI 66 CGGCCG CG[GA][CT]^CG
998 CGGCCG
BsmI 1011 GAATGC GAATGC(1/-1)
743 GCATTC
BsrFI 166 GCCGGC [GA]^CCGG[CT]
1393 ACCGGC
Cfr10I 166 GCCGGC [GA]^CCGG[CT]
1393 ACCGGC
EagI 66 CGGCCG C^GGCCG
998 CGGCCG
EclXI 66 CGGCCG C^GGCCG
998 CGGCCG
Eco52I 66 CGGCCG C^GGCCG
998 CGGCCG
FbaI 923 TGATCA T^GATCA
1154 TGATCA
Hin1I 1117 GGCGCC G[GA]^CG[CT]C
1220 GGCGCC
HinfI 480 GACTC G^A[ACGT]TC
880 GAGTC
KasI 1117 GGCGCC G^GCGCC
1220 GGCGCC
MaeIII 856 GTCAC ^GT[ACGT]AC
1342 GTGAC
MboII 573 GAAGA GAAGA(8/7)
633 GAAGA
MseI 539 TTAA T^TAA
833 TTAA
MslI 234 CACCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
768 CACCTCCGTG
NarI 1117 GGCGCC GG^CGCC
1220 GGCGCC
PleI 480 GACTC GAGTC(4/5)
880 GAGTC
PpuMI 800 GGGACCT [GA]G^G[AT]CC[CT]
1313 GGGACCT
RsaI 888 GTAC GT^AC
1164 GTAC
SfoI 1117 GGCGCC GGC^GCC
1220 GGCGCC
Tru9I 539 TTAA T^TAA
833 TTAA
Tsp45I 856 GTCAC ^GT[GC]AC
1342 GTGAC
XcmI 1133 CCAAGCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1184 CCAAGCTGGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstAPI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
KspI MaeII MboI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PstI PvuII
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI TspRI TthHB8I