(Circular) (Six-base) MAPSORT of: pFN21AB0457.seq Check: 3334 from: 1 to: 6220 pFN21AB0457 6220 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3443 3443 Size: 6220 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3563 1937 Size: 1626 4594 Acc65I G'GTAC_C Cuts at: 2892 3541 2892 Size: 649 5571 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2023 2657 4283 5667 5935 1632 Size: 391 634 1626 1384 268 1917 Fragments arranged by size: 1917 1626 1384 634 391 268 AfeI AGC'GCT Cuts at: 3367 3367 Size: 6220 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 5119 6096 1203 Size: 3916 977 1327 Fragments arranged by size: 3916 1327 977 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 2243 2777 1166 Size: 1077 534 4609 Fragments arranged by size: 4609 1077 534 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6220 AlwNI CAG_nnn'CTG Cuts at: 2998 3350 3360 3507 3963 5535 2998 Size: 352 10 147 456 1572 3683 Fragments arranged by size: 3683 1572 456 352 147 10 ApaI G_GGCC'C Cuts at: 2223 2223 Size: 6220 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 3529 3755 3907 6016 1493 Size: 2036 226 152 2109 1697 Fragments arranged by size: 2109 2036 1697 226 152 AscI GG'CGCG_CC Cuts at: 2032 2032 Size: 6220 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 3297 3545 1870 Size: 73 1354 248 4545 Fragments arranged by size: 4545 1354 248 73 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2892 3122 3225 3541 4996 Size: 325 217 627 1105 230 103 316 1455 Cuts at: 4996 618 Size: 1842 Fragments arranged by size: 1842 1455 1105 627 325 316 230 217 103 BanII G_rGCy'C Cuts at: 1391 2223 2463 3464 3539 1391 Size: 832 240 1001 75 4072 Fragments arranged by size: 4072 1001 832 240 75 BbeI G_GCGC'C Cuts at: 3126 3229 3126 Size: 103 6117 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4411 4445 1903 Size: 34 2474 34 3678 Fragments arranged by size: 3678 2474 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 2928 3159 1548 Size: 1380 231 4609 Fragments arranged by size: 4609 1380 231 BfrBI ATG'CAT Cuts at: 2235 2235 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3053 4831 136 Size: 107 122 71 631 605 1381 1778 1525 Fragments arranged by size: 1778 1525 1381 631 605 122 107 71 BglII A'GATC_T Cuts at: 6215 6215 Size: 6220 BlpI GC'TnA_GC Cuts at: 3637 3637 Size: 6220 Bme1580I G_kGCm'C Cuts at: 1790 2223 4281 5437 1790 Size: 433 2058 1156 2573 Fragments arranged by size: 2573 2058 1156 433 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2217 3052 3951 Size: 416 53 83 399 24 800 835 899 Cuts at: 3951 4909 442 Size: 958 1753 Fragments arranged by size: 1753 958 899 835 800 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2303 2405 2426 2477 2585 2699 2738 3062 Size: 1064 102 21 51 108 114 39 324 Cuts at: 3062 3137 3254 4880 1239 Size: 75 117 1626 2579 Fragments arranged by size: 2579 1626 1064 324 117 114 108 102 75 51 39 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4345 5210 5508 4345 Size: 865 298 5057 Fragments arranged by size: 5057 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2936 4883 882 Size: 617 1437 1947 2219 Fragments arranged by size: 2219 1947 1437 617 BsaAI yAC'GTr Cuts at: 493 1204 2551 4011 493 Size: 711 1347 1460 2702 Fragments arranged by size: 2702 1460 1347 711 BsaBI GATnn'nnATC Cuts at: 6214 6214 Size: 6220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3123 3226 4027 4409 Size: 53 83 186 936 1590 103 801 382 Cuts at: 4409 5044 275 Size: 635 1451 Fragments arranged by size: 1590 1451 936 801 635 382 186 103 83 53 BsaWI w'CCGG_w Cuts at: 4653 5051 5325 5472 5883 4653 Size: 398 274 147 411 4990 Fragments arranged by size: 4990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2450 2480 2489 2519 1178 Size: 30 165 30 1047 30 9 30 4879 Fragments arranged by size: 4879 1047 165 30 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 2333 2603 2606 2750 2753 2924 2978 Size: 1069 78 270 3 144 3 171 54 Cuts at: 2978 3257 1186 Size: 279 4149 Fragments arranged by size: 4149 1069 279 270 171 144 78 54 3 3 BseYI C'CCAG_C Cuts at: 3193 5423 5919 3193 Size: 2230 496 3494 Fragments arranged by size: 3494 2230 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2573 1163 Size: 573 837 4810 Fragments arranged by size: 4810 837 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2074 3006 3670 4431 5459 1540 Size: 275 186 73 932 664 761 1028 2301 Fragments arranged by size: 2301 1028 932 761 664 275 186 73 BsiHKAI G_wGCw'C Cuts at: 3398 4281 4366 5437 3398 Size: 883 85 1071 4181 Fragments arranged by size: 4181 1071 883 85 BsmI GAATG_Cn' Cuts at: 2748 3022 3731 3824 2748 Size: 274 709 93 5144 Fragments arranged by size: 5144 709 274 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2463 3398 3464 3539 4281 4366 Size: 399 433 240 935 66 75 742 85 Cuts at: 4366 5437 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 935 742 433 399 240 85 75 66 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2018 2340 3443 3575 844 Size: 1174 322 1103 132 3489 Fragments arranged by size: 3489 1174 1103 322 132 BsrBI CCG'CTC Cuts at: 1394 1823 2202 4107 1394 Size: 429 379 1905 3507 Fragments arranged by size: 3507 1905 429 379 BsrDI GCAATG_nn' Cuts at: 65 1248 2826 4715 4889 65 Size: 1183 1578 1889 174 1396 Fragments arranged by size: 1889 1578 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2171 3398 4864 5051 1700 Size: 110 361 1227 1466 187 2869 Fragments arranged by size: 2869 1466 1227 361 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 2032 1925 Size: 107 6113 BssSI C'ACGA_G Cuts at: 4280 5292 4280 Size: 1012 5208 BstAPI GCAn_nnn'nTGC Cuts at: 794 2112 2560 3360 3507 794 Size: 1318 448 800 147 3507 Fragments arranged by size: 3507 1318 800 448 147 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6220 BstYI r'GATC_y Cuts at: 1972 2742 3550 3917 4302 4319 5771 5857 5869 Size: 770 808 367 385 17 1452 86 12 Cuts at: 5869 6215 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 808 770 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2720 2720 Size: 6220 BtgI C'CryG_G Cuts at: 513 1065 2063 2101 2119 2778 2922 3048 513 Size: 552 998 38 18 659 144 126 3685 Fragments arranged by size: 3685 998 659 552 144 126 38 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 2309 3361 3736 4530 4550 2309 Size: 1052 375 794 20 3979 Fragments arranged by size: 3979 1052 794 375 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DraIII CAC_nnn'GTG Cuts at: 2152 2152 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2858 5227 809 Size: 507 1542 2369 1802 Fragments arranged by size: 2369 1802 1542 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2071 3003 3108 Size: 54 1425 50 275 18 241 932 105 Cuts at: 3108 3341 3418 3667 4556 6185 8 Size: 233 77 249 889 1629 43 Fragments arranged by size: 1629 1425 932 889 275 249 241 233 105 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2071 3003 3667 1537 Size: 275 259 932 664 4090 Fragments arranged by size: 4090 932 664 275 259 EarI CTCTTCn'nnn_ Cuts at: 1353 4150 1353 Size: 2797 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4807 5181 5327 387 Size: 1064 138 3218 374 146 1280 Fragments arranged by size: 3218 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2023 2303 2405 2426 2477 2585 2657 Size: 393 391 280 102 21 51 108 72 Cuts at: 2657 2699 2738 3062 3137 3254 4283 4880 5667 Size: 42 39 324 75 117 1029 597 787 Cuts at: 5667 5935 1239 Size: 268 1524 Fragments arranged by size: 1524 1029 787 597 393 391 324 280 268 117 108 102 75 72 51 42 39 21 EcoNI CCTnn'n_nnAGG Cuts at: 2468 2702 3131 3233 2468 Size: 234 429 102 5455 Fragments arranged by size: 5455 429 234 102 EcoO109I rG'GnC_Cy Cuts at: 2695 2806 3319 2695 Size: 111 513 5596 Fragments arranged by size: 5596 513 111 EcoRI G'AATT_C Cuts at: 3529 3907 3529 Size: 378 5842 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2395 2395 Size: 6220 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 3126 3229 3369 5367 1678 Size: 1448 103 140 1998 2531 Fragments arranged by size: 2531 1998 1448 140 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2870 2902 4861 4893 4935 Size: 32 135 32 1298 32 1959 32 42 Cuts at: 4935 4967 1373 Size: 32 2626 Fragments arranged by size: 2626 1959 1298 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2254 3564 3810 669 Size: 988 281 316 1310 246 3079 Fragments arranged by size: 3079 1310 988 316 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KasI G'GCGC_C Cuts at: 3122 3225 3122 Size: 103 6117 KpnI G_GTAC'C Cuts at: 2896 3545 2896 Size: 649 5571 MfeI C'AATT_G Cuts at: 3422 3819 3422 Size: 397 5823 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5334 5518 1305 Size: 4029 184 2007 Fragments arranged by size: 4029 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4398 Fragments arranged by size: 4398 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 2777 4178 4537 4696 518 Size: 648 1077 534 1401 359 159 2042 Fragments arranged by size: 2042 1401 1077 648 534 359 159 MspA1I CmG'CkG Cuts at: 1606 2043 2065 2076 2121 2523 2615 3050 3099 Size: 437 22 11 45 402 92 435 49 Cuts at: 3099 3507 3636 4313 5461 5706 1606 Size: 408 129 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 437 435 408 402 245 129 92 49 45 22 11 NaeI GCC'GGC Cuts at: 2173 2173 Size: 6220 NarI GG'CG_CC Cuts at: 3123 3226 3123 Size: 103 6117 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5668 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NgoMIV G'CCGG_C Cuts at: 2171 2171 Size: 6220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NsiI A_TGCA'T Cuts at: 2237 2237 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 3578 5123 1132 Size: 97 2349 1545 2229 Fragments arranged by size: 2349 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3481 1348 Size: 2133 4087 PciI A'CATG_T Cuts at: 5119 5119 Size: 6220 PflMI CCAn_nnn'nTGG Cuts at: 1108 3350 1108 Size: 2242 3978 PfoI T'CCnGG_A Cuts at: 2382 2703 2382 Size: 321 5899 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PmlI CAC'GTG Cuts at: 2551 2551 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2806 3319 2806 Size: 513 5707 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PspOMI G'GGCC_C Cuts at: 2219 2219 Size: 6220 PstI C_TGCA'G Cuts at: 830 2421 3132 3365 3572 830 Size: 1591 711 233 207 3478 Fragments arranged by size: 3478 1591 711 233 207 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2043 2615 3507 2043 Size: 572 892 4756 Fragments arranged by size: 4756 892 572 SacII CC_GC'GG Cuts at: 2066 2122 3051 2066 Size: 56 929 5235 Fragments arranged by size: 5235 929 56 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SfcI C'TryA_G Cuts at: 826 1045 2417 3128 3220 3361 3568 4703 5384 Size: 219 1372 711 92 141 207 1135 681 Cuts at: 5384 5575 826 Size: 191 1471 Fragments arranged by size: 1471 1372 1135 711 681 219 207 191 141 92 SfiI GGCCn_nnn'nGGCC Cuts at: 3053 3053 Size: 6220 SfoI GGC'GCC Cuts at: 3124 3227 3124 Size: 103 6117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 3299 3547 1872 Size: 1427 248 4545 Fragments arranged by size: 4545 1427 248 SmlI C'TyrA_G Cuts at: 820 1017 1943 4324 5225 5487 820 Size: 197 926 2381 901 262 1553 Fragments arranged by size: 2381 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 2958 3659 513 Size: 552 1893 701 3074 Fragments arranged by size: 3074 1893 701 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2239 3473 4247 4264 4417 4602 5047 Size: 1255 659 1234 774 17 153 185 445 Cuts at: 5047 325 Size: 1498 Fragments arranged by size: 1498 1255 1234 774 659 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4466 6178 96 Size: 274 80 33 51 1443 2489 1712 138 Fragments arranged by size: 2489 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2489 2521 6180 6212 2489 Size: 32 3659 32 2497 Fragments arranged by size: 3659 2497 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2858 1316 Size: 1542 4678 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3145 3196 3145 Size: 51 6169 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3297 3545 1870 Size: 1427 248 4545 Fragments arranged by size: 4545 1427 248 XmnI GAAnn'nnTTC Cuts at: 4349 4349 Size: 6220 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI NruI PacI PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI