Restriction map for
pFN21AB0457
(Linear) (Six-base) MAPSORT of: KIBB0457.seq Check: 3019 from: 1 to: 1515
KIBB0457.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1438 1515
Size: 1438 77
Acc65I G'GTAC_C
Cuts at: 0 887 1515
Size: 887 628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 652 1515
Size: 18 634 863
Fragments arranged by size:
863 634 18
AfeI AGC'GCT
Cuts at: 0 1362 1515
Size: 1362 153
AleI CACnn'nnGTG
Cuts at: 0 238 772 1515
Size: 238 534 743
Fragments arranged by size:
743 534 238
AlwNI CAG_nnn'CTG
Cuts at: 0 993 1345 1355 1502 1515
Size: 993 352 10 147 13
Fragments arranged by size:
993 352 147 13 10
ApaI G_GGCC'C
Cuts at: 0 218 1515
Size: 218 1297
AscI GG'CGCG_CC
Cuts at: 0 27 1515
Size: 27 1488
AvaI C'yCGr_G
Cuts at: 0 1292 1515
Size: 1292 223
BanI G'GyrC_C
Cuts at: 0 887 1117 1220 1515
Size: 887 230 103 295
Fragments arranged by size:
887 295 230 103
BanII G_rGCy'C
Cuts at: 0 218 458 1459 1515
Size: 218 240 1001 56
Fragments arranged by size:
1001 240 218 56
BbeI G_GCGC'C
Cuts at: 0 1121 1224 1515
Size: 1121 103 291
Fragments arranged by size:
1121 291 103
BclI T'GATC_A
Cuts at: 0 923 1154 1515
Size: 923 231 361
Fragments arranged by size:
923 361 231
BfrBI ATG'CAT
Cuts at: 0 230 1515
Size: 230 1285
BglI GCCn_nnn'nGGC
Cuts at: 0 1048 1515
Size: 1048 467
Bme1580I G_kGCm'C
Cuts at: 0 218 1515
Size: 218 1297
BmrI ACTGGGnnnn_n'
Cuts at: 0 212 1047 1515
Size: 212 835 468
Fragments arranged by size:
835 468 212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 400 421 472 580 694 733 1057
Size: 298 102 21 51 108 114 39 324
Cuts at: 1057 1132 1249 1515
Size: 75 117 266
Fragments arranged by size:
324 298 266 117 114 108 102 75
51 39 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 931 1515
Size: 931 584
BsaAI yAC'GTr
Cuts at: 0 546 1515
Size: 546 969
BsaHI Gr'CG_yC
Cuts at: 0 1118 1221 1515
Size: 1118 103 294
Fragments arranged by size:
1118 294 103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 445 475 484 514 1515
Size: 445 30 9 30 1001
Fragments arranged by size:
1001 445 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 328 598 601 745 748 919 973
Size: 250 78 270 3 144 3 171 54
Cuts at: 973 1252 1515
Size: 279 263
Fragments arranged by size:
279 270 263 250 171 144 78 54
3 3
BseYI C'CCAG_C
Cuts at: 0 1188 1515
Size: 1188 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1515
Size: 568 947
BsiEI CG_ry'CG
Cuts at: 0 69 1001 1515
Size: 69 932 514
Fragments arranged by size:
932 514 69
BsiHKAI G_wGCw'C
Cuts at: 0 1393 1515
Size: 1393 122
BsmI GAATG_Cn'
Cuts at: 0 743 1017 1515
Size: 743 274 498
Fragments arranged by size:
743 498 274
Bsp1286I G_dGCh'C
Cuts at: 0 218 458 1393 1459 1515
Size: 218 240 935 66 56
Fragments arranged by size:
935 240 218 66 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 13 335 1438 1515
Size: 13 322 1103 77
Fragments arranged by size:
1103 322 77 13
BsrBI CCG'CTC
Cuts at: 0 197 1515
Size: 197 1318
BsrDI GCAATG_nn'
Cuts at: 0 821 1515
Size: 821 694
BsrFI r'CCGG_y
Cuts at: 0 166 1393 1515
Size: 166 1227 122
Fragments arranged by size:
1227 166 122
BssHII G'CGCG_C
Cuts at: 0 27 1515
Size: 27 1488
BstAPI GCAn_nnn'nTGC
Cuts at: 0 107 555 1355 1502 1515
Size: 107 448 800 147 13
Fragments arranged by size:
800 448 147 107 13
BstYI r'GATC_y
Cuts at: 0 737 1515
Size: 737 778
Bsu36I CC'TnA_GG
Cuts at: 0 715 1515
Size: 715 800
BtgI C'CryG_G
Cuts at: 0 58 96 114 773 917 1043 1515
Size: 58 38 18 659 144 126 472
Fragments arranged by size:
659 472 144 126 58 38 18
BtsI GCAGTG_nn'
Cuts at: 0 304 1356 1515
Size: 304 1052 159
Fragments arranged by size:
1052 304 159
DraIII CAC_nnn'GTG
Cuts at: 0 147 1515
Size: 147 1368
DrdI GACnn_nn'nnGTC
Cuts at: 0 853 1515
Size: 853 662
EaeI y'GGCC_r
Cuts at: 0 66 998 1103 1336 1413 1515
Size: 66 932 105 233 77 102
Fragments arranged by size:
932 233 105 102 77 66
EagI C'GGCC_G
Cuts at: 0 66 998 1515
Size: 66 932 517
Fragments arranged by size:
932 517 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 298 400 421 472 580 652 694
Size: 18 280 102 21 51 108 72 42
Cuts at: 694 733 1057 1132 1249 1515
Size: 39 324 75 117 266
Fragments arranged by size:
324 280 266 117 108 102 75 72
51 42 39 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 463 697 1126 1228 1515
Size: 463 234 429 102 287
Fragments arranged by size:
463 429 287 234 102
EcoO109I rG'GnC_Cy
Cuts at: 0 690 801 1314 1515
Size: 690 111 513 201
Fragments arranged by size:
690 513 201 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 390 1515
Size: 390 1125
HaeII r_GCGC'y
Cuts at: 0 1121 1224 1364 1515
Size: 1121 103 140 151
Fragments arranged by size:
1121 151 140 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 865 897 1515
Size: 865 32 618
Fragments arranged by size:
865 618 32
HincII GTy'rAC
Cuts at: 0 249 1515
Size: 249 1266
KasI G'GCGC_C
Cuts at: 0 1117 1220 1515
Size: 1117 103 295
Fragments arranged by size:
1117 295 103
KpnI G_GTAC'C
Cuts at: 0 891 1515
Size: 891 624
MfeI C'AATT_G
Cuts at: 0 1417 1515
Size: 1417 98
MslI CAynn'nnrTG
Cuts at: 0 238 772 1515
Size: 238 534 743
Fragments arranged by size:
743 534 238
MspA1I CmG'CkG
Cuts at: 0 38 60 71 116 518 610 1045 1094
Size: 38 22 11 45 402 92 435 49
Cuts at: 1094 1502 1515
Size: 408 13
Fragments arranged by size:
435 408 402 92 49 45 38 22
13 11
NaeI GCC'GGC
Cuts at: 0 168 1515
Size: 168 1347
NarI GG'CG_CC
Cuts at: 0 1118 1221 1515
Size: 1118 103 294
Fragments arranged by size:
1118 294 103
NgoMIV G'CCGG_C
Cuts at: 0 166 1515
Size: 166 1349
NsiI A_TGCA'T
Cuts at: 0 232 1515
Size: 232 1283
PasI CC'CwG_GG
Cuts at: 0 1476 1515
Size: 1476 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 1345 1515
Size: 1345 170
PfoI T'CCnGG_A
Cuts at: 0 377 698 1515
Size: 377 321 817
Fragments arranged by size:
817 377 321
PmlI CAC'GTG
Cuts at: 0 546 1515
Size: 546 969
PpuMI rG'GwC_Cy
Cuts at: 0 801 1314 1515
Size: 801 513 201
Fragments arranged by size:
801 513 201
PspOMI G'GGCC_C
Cuts at: 0 214 1515
Size: 214 1301
PstI C_TGCA'G
Cuts at: 0 416 1127 1360 1515
Size: 416 711 233 155
Fragments arranged by size:
711 416 233 155
PvuII CAG'CTG
Cuts at: 0 38 610 1502 1515
Size: 38 572 892 13
Fragments arranged by size:
892 572 38 13
SacII CC_GC'GG
Cuts at: 0 61 117 1046 1515
Size: 61 56 929 469
Fragments arranged by size:
929 469 61 56
SfcI C'TryA_G
Cuts at: 0 412 1123 1215 1356 1515
Size: 412 711 92 141 159
Fragments arranged by size:
711 412 159 141 92
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1048 1515
Size: 1048 467
SfoI GGC'GCC
Cuts at: 0 1119 1222 1515
Size: 1119 103 293
Fragments arranged by size:
1119 293 103
SmaI CCC'GGG
Cuts at: 0 1294 1515
Size: 1294 221
StyI C'CwwG_G
Cuts at: 0 953 1515
Size: 953 562
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 234 1468 1515
Size: 234 1234 47
Fragments arranged by size:
1234 234 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 484 516 1515
Size: 484 32 999
Fragments arranged by size:
999 484 32
Tth111I GACn'n_nGTC
Cuts at: 0 853 1515
Size: 853 662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1140 1191 1515
Size: 1140 51 324
Fragments arranged by size:
1140 324 51
XmaI C'CCGG_G
Cuts at: 0 1292 1515
Size: 1292 223
Enzymes that do cut:
AarI Acc65I AcuI AfeI AleI AlwNI ApaI AscI
AvaI BanI BanII BbeI BclI BfrBI BglI Bme1580I
BmrI BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EagI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII KasI KpnI MfeI MslI MspA1I NaeI
NarI NgoMIV NsiI PasI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacII SfcI SfiI SfoI SmaI
StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AlfI
AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaBI BsaWI BsiWI BsmBI BspEI
BspHI BsrGI BssSI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI EarI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI MluI MmeI MscI
NcoI NdeI NheI NotI NruI NspI PacI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XhoI XmnI ZraI