Restriction map for pFN21AB0408
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# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AhdI        ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BbeI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BsaHI       
BsaWI       BseAI       BsiWI       BspDI       BspEI       
BspHI       BspLU11I    BsrDI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MfeI        MluI        MroI        MunI        NarI        
NcoI        NdeI        NheI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        TaqI        Tsp509I     TthHB8I     
XbaI        XhoI        


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# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccI         1876  GTAGAC               GT^[AC][GT]AC
AflIII       1505  ACGTGT               A^C[GA][CT]GT
AocI         1454  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1682  AGCGCT               AGC^GCT
ApaI         1414  GGGCCC               GGGCC^C
AspI         1981  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1481  GGATCC               G^GATCC
BglI         2289  GCCTGCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        1398  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        2071  GAGACG               CGTCTC(1/5)
BsmI         1565  GCATTC               GAATGC(1/-1)
Bsp1407I      230  TGTACA               T^GTACA
BsrBI        1272  CCGCTC               CCGCTC(-3/-3)
BsrGI         230  TGTACA               T^GTACA
BssHII       1162  GCGCGC               G^CGCGC
Bsu36I       1454  CCTCAGG              CC^T[ACGT]AGG
DsaI          281  CCGTGG               C^C[GA][CT]GG
Eco47III     1682  AGCGCT               AGC^GCT
Eco81I       1454  CCTCAGG              CC^T[ACGT]AGG
MamI         1398  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          104  TTAA                 T^TAA
NaeI          348  GCCGGC               GCC^GGC
NgoMIV        348  GCCGGC               G^CCGGC
NotI         1268  GCGGCCGC             GC^GGCCGC
NruI         2334  TCGCGA               TCG^CGA
PflFI        1981  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
ScaI          140  AGTACT               AGT^ACT
SexAI        1367  ACCAGGT              A^CC[AT]GGT
SspBI         230  TGTACA               T^GTACA
Tru9I         104  TTAA                 T^TAA
Tth111I      1981  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          141  GTAC                 GT^AC
              231  GTAC                
BcgI         1691  GCAAGAGATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1775  GCAAGGCCTTCG        
BsaI         1312  GAGACC               GGTCTC(1/5)
             2368  GAGACC              
BsrFI         348  GCCGGC               [GA]^CCGG[CT]
             2043  GCCGGT              
BssSI         603  CACGAG               CACGAG(-5/-1)
             1002  CTCGTG              
BstXI        1709  CCAGCCAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2186  CCAACAGCCTGG        
Cfr10I        348  GCCGGC               [GA]^CCGG[CT]
             2043  GCCGGT              
EagI         1037  CGGCCG               C^GGCCG
             1269  CGGCCG              
EclXI        1037  CGGCCG               C^GGCCG
             1269  CGGCCG              
Eco52I       1037  CGGCCG               C^GGCCG
             1269  CGGCCG              
EcoNI         207  CCTGGGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              492  CCTCAAGGAGG         
EcoT14I       380  CCTTGG               C^C[AT][AT]GG
              893  CCAAGG              
NspI           24  GCATGT               [GA]CATG^[CT]
             2202  GCATGT              
PflMI        1221  CCACCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2223  CCAGGCACTGG         
PpuMI         488  GGGACCT              [GA]G^G[AT]CC[CT]
             1796  GGGACCT             
PshAI        2016  GACAGCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2073  GACGGAAGTC          
RsaI          141  GTAC                 GT^AC
              231  GTAC                
SmaI          566  CCCGGG               CCC^GGG
              851  CCCGGG              
StyI          380  CCTTGG               C^C[AT][AT]GG
              893  CCAAGG              
TfiI         1697  GATTC                G^A[AT]TC
             2210  GAATC               
Van91I       1221  CCACCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2223  CCAGGCACTGG         
XcmI          602  CCACGAGTGGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2275  CCAGGTGGCACGTGG     
XmaCI         566  CCCGGG               C^CCGGG
              851  CCCGGG              
XmaI          566  CCCGGG               C^CCGGG
              851  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccII       AciI        AluI        Alw44I      AlwI        
AlwNI       ApaLI       Asp700I     AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbrPI       
BbsI        BbvCI       BbvI        BcnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PmaCI       PmlI        
PstI        PvuII       SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        TseI        Tsp45I      TspRI       XhoII       
XmnI        
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