Restriction map for pFN21AB0408
 (Linear) (Six-base) MAPSORT of: KIBB0408.seq  Check: 6184  from: 1  to: 2409



KIBB0408.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     204    2289    2409 
   Size:        204    2085     120

  Fragments arranged by size: 

                2085     204     120
 
AccI GT'mk_AC

Cuts at:      0    1877    2409 
   Size:       1877     532
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1096    1402    1414    1486    2104    2409 
   Size:       1096     306      12      72     618     305

  Fragments arranged by size: 

                1096     618     306     305      72      12
 
AfeI AGC'GCT

Cuts at:      0    1684    2409 
   Size:       1684     725
 
AflIII A'CryG_T

Cuts at:      0    1505    2409 
   Size:       1505     904
 
AleI CACnn'nnGTG

Cuts at:      0      82    1574    1758    2038    2409 
   Size:         82    1492     184     280     371

  Fragments arranged by size: 

                1492     371     280     184      82
 
AlwNI CAG_nnn'CTG

Cuts at:      0     219     628    2229    2304    2386    2409 
   Size:        219     409    1601      75      82      23

  Fragments arranged by size: 

                1601     409     219      82      75      23
 
ApaI G_GGCC'C

Cuts at:      0    1418    2409 
   Size:       1418     991
 
ApaLI G'TGCA_C

Cuts at:      0     178    1231    1906    2409 
   Size:        178    1053     675     503

  Fragments arranged by size: 

                1053     675     503     178
 
AvaI C'yCGr_G

Cuts at:      0     566     787     825     851    1152    1203    1942    2409 
   Size:        566     221      38      26     301      51     739     467

  Fragments arranged by size: 

                 739     566     467     301     221      51      38      26
            
 
BamHI G'GATC_C

Cuts at:      0    1481    2409 
   Size:       1481     928
 
BanI G'GyrC_C

Cuts at:      0     868    1472    2040    2409 
   Size:        868     604     568     369

  Fragments arranged by size: 

                 868     604     568     369
 
BanII G_rGCy'C

Cuts at:      0     256     590     652     677     839    1418    1943    2409 
   Size:        256     334      62      25     162     579     525     466

  Fragments arranged by size: 

                 579     525     466     334     256     162      62      25
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1066    1906    2132    2316    2409 
   Size:       1066     840     226     184      93

  Fragments arranged by size: 

                1066     840     226     184      93
 
BbvCI CC'TCA_GC

Cuts at:      0     629     743    2345    2409 
   Size:        629     114    1602      64

  Fragments arranged by size: 

                1602     629     114      64
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1680    1714    1764    1798    2409 
   Size:       1680      34      50      34     611

  Fragments arranged by size: 

                1680     611      50      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2295    2409 
   Size:       2295     114
 
BlpI GC'TnA_GC

Cuts at:      0     244     344     439    2198    2409 
   Size:        244     100      95    1759     211

  Fragments arranged by size: 

                1759     244     211     100      95
 
Bme1580I G_kGCm'C

Cuts at:      0     182     820    1235    1345    1418    1910    2409 
   Size:        182     638     415     110      73     492     499

  Fragments arranged by size: 

                 638     499     492     415     182     110      73
 
BmgBI CAC'GTC

Cuts at:      0     174    1560    2409 
   Size:        174    1386     849

  Fragments arranged by size: 

                1386     849     174
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     858    2409 
   Size:        858    1551
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     469     790    1330    1847    1957    2409 
   Size:        241     228     321     540     517     110     452

  Fragments arranged by size: 

                 540     517     452     321     241     228     110
 
Bpu10I CC'TnA_GC

Cuts at:      0     250     629     743     864    2345    2409 
   Size:        250     379     114     121    1481      64

  Fragments arranged by size: 

                1481     379     250     121     114      64
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     478     574    1198    1405    2409 
   Size:        316     162      96     624     207    1004

  Fragments arranged by size: 

                1004     624     316     207     162      96
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1306    2362    2409 
   Size:       1306    1056      47

  Fragments arranged by size: 

                1306    1056      47
 
BsaAI yAC'GTr

Cuts at:      0    1506    1644    1896    2285    2409 
   Size:       1506     138     252     389     124

  Fragments arranged by size: 

                1506     389     252     138     124
 
BsaBI GATnn'nnATC

Cuts at:      0    1402    2409 
   Size:       1402    1007
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     214     244    1324    1354    1873    1903    2042    2072 
   Size:        214      30    1080      30     519      30     139      30

Cuts at:   2072    2409 
   Size:        337

  Fragments arranged by size: 

                1080     519     337     214     139      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     430     514     709     727     736     739     769     805 
   Size:        430      84     195      18       9       3      30      36

Cuts at:    805     820    1108    1960    2409 
   Size:         15     288     852     449

  Fragments arranged by size: 

                 852     449     430     288     195      84      36      30
                  18      15       9       3
 
BseYI C'CCAG_C

Cuts at:      0      11      57     481     764    2311    2409 
   Size:         11      46     424     283    1547      98

  Fragments arranged by size: 

                1547     424     283      98      46      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     557    1035    1120    1782    1791    2137    2409 
   Size:         60     497     478      85     662       9     346     272

  Fragments arranged by size: 

                 662     497     478     346     272      85      60       9
            
 
BsiEI CG_ry'CG

Cuts at:      0     882    1040    1272    2409 
   Size:        882     158     232    1137

  Fragments arranged by size: 

                1137     882     232     158
 
BsiHKAI G_wGCw'C

Cuts at:      0     182     590     652     839    1235    1337    1910    2409 
   Size:        182     408      62     187     396     102     573     499

  Fragments arranged by size: 

                 573     499     408     396     187     182     102      62
            
 
BsmI GAATG_Cn'

Cuts at:      0    1565    2409 
   Size:       1565     844
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2065    2409 
   Size:       2065     344
 
Bsp1286I G_dGCh'C

Cuts at:      0     182     256     590     652     677     820     839    1235 
   Size:        182      74     334      62      25     143      19     396

Cuts at:   1235    1337    1345    1418    1910    1943    2409 
   Size:        102       8      73     492      33     466

  Fragments arranged by size: 

                 492     466     396     334     182     143     102      74
                  73      62      33      25      19       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     204    1142    1892    2289    2409 
   Size:        204     938     750     397     120

  Fragments arranged by size: 

                 938     750     397     204     120
 
BsrBI CCG'CTC

Cuts at:      0    1274    2409 
   Size:       1274    1135
 
BsrFI r'CCGG_y

Cuts at:      0     348    2043    2409 
   Size:        348    1695     366

  Fragments arranged by size: 

                1695     366     348
 
BsrGI T'GTAC_A

Cuts at:      0     230    2409 
   Size:        230    2179
 
BssHII G'CGCG_C

Cuts at:      0    1162    2409 
   Size:       1162    1247
 
BssSI C'ACGA_G

Cuts at:      0     603    1002    2409 
   Size:        603     399    1407

  Fragments arranged by size: 

                1407     603     399
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1517    1769    2304    2409 
   Size:       1517     252     535     105

  Fragments arranged by size: 

                1517     535     252     105
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1716    2193    2409 
   Size:       1716     477     216

  Fragments arranged by size: 

                1716     477     216
 
BstYI r'GATC_y

Cuts at:      0    1397    1481    1931    2409 
   Size:       1397      84     450     478

  Fragments arranged by size: 

                1397     478     450      84
 
Bsu36I CC'TnA_GG

Cuts at:      0    1455    2409 
   Size:       1455     954
 
BtgI C'CryG_G

Cuts at:      0     281    2409 
   Size:        281    2128
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1389    1620    2409 
   Size:       1389     231     789

  Fragments arranged by size: 

                1389     789     231
 
BtsI GCAGTG_nn'

Cuts at:      0     125    1347    1515    1959    2063    2409 
   Size:        125    1222     168     444     104     346

  Fragments arranged by size: 

                1222     444     346     168     125     104
 
EaeI y'GGCC_r

Cuts at:      0     236     278     284     383     425     527    1037    1269 
   Size:        236      42       6      99      42     102     510     232

Cuts at:   1269    1718    2409 
   Size:        449     691

  Fragments arranged by size: 

                 691     510     449     236     232     102      99      42
                  42       6
 
EagI C'GGCC_G

Cuts at:      0    1037    1269    2409 
   Size:       1037     232    1140

  Fragments arranged by size: 

                1140    1037     232
 
EarI CTCTTCn'nnn_

Cuts at:      0     160     686    1099    1198    2409 
   Size:        160     526     413      99    1211

  Fragments arranged by size: 

                1211     526     413     160      99
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1738    2317    2409 
   Size:       1738     579      92

  Fragments arranged by size: 

                1738     579      92
 
EcoICRI GAG'CTC

Cuts at:      0     588     650     837    2409 
   Size:        588      62     187    1572

  Fragments arranged by size: 

                1572     588     187      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     469     790    1096    1330    1402    1414    1486 
   Size:        241     228     321     306     234      72      12      72

Cuts at:   1486    1847    1957    2104    2409 
   Size:        361     110     147     305

  Fragments arranged by size: 

                 361     321     306     305     241     234     228     147
                 110      72      72      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     211     496    2409 
   Size:        211     285    1913

  Fragments arranged by size: 

                1913     285     211
 
EcoO109I rG'GnC_Cy

Cuts at:      0     478     489     783     855    1415    1797    2409 
   Size:        478      11     294      72     560     382     612

  Fragments arranged by size: 

                 612     560     478     382     294      72      11
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     171    2409 
   Size:        171    2238
 
HaeII r_GCGC'y

Cuts at:      0    1221    1547    1686    2409 
   Size:       1221     326     139     723

  Fragments arranged by size: 

                1221     723     326     139
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1055    1307    2409 
   Size:       1055     252    1102

  Fragments arranged by size: 

                1102    1055     252
 
MscI TGG'CCA

Cuts at:      0     286     385     427    1720    2409 
   Size:        286      99      42    1293     689

  Fragments arranged by size: 

                1293     689     286      99      42
 
MslI CAynn'nnrTG

Cuts at:      0      82    1574    1590    1758    2038    2409 
   Size:         82    1492      16     168     280     371

  Fragments arranged by size: 

                1492     371     280     168      82      16
 
MspA1I CmG'CkG

Cuts at:      0      39      51      57     533     742     768     814    1172 
   Size:         39      12       6     476     209      26      46     358

Cuts at:   1172    1227    1238    1490    1554    2409 
   Size:         55      11     252      64     855

  Fragments arranged by size: 

                 855     476     358     252     209      64      55      46
                  39      26      12      11       6
 
NaeI GCC'GGC

Cuts at:      0     350    2409 
   Size:        350    2059
 
NgoMIV G'CCGG_C

Cuts at:      0     348    2409 
   Size:        348    2061
 
NotI GC'GGCC_GC

Cuts at:      0    1269    2409 
   Size:       1269    1140
 
NruI TCG'CGA

Cuts at:      0    2336    2409 
   Size:       2336      73
 
NspI r_CATG'y

Cuts at:      0      28    2206    2409 
   Size:         28    2178     203

  Fragments arranged by size: 

                2178     203      28
 
PasI CC'CwG_GG

Cuts at:      0     361    2409 
   Size:        361    2048
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1227    2229    2409 
   Size:       1227    1002     180

  Fragments arranged by size: 

                1227    1002     180
 
PfoI T'CCnGG_A

Cuts at:      0    1934    2409 
   Size:       1934     475
 
PmlI CAC'GTG

Cuts at:      0    1644    1896    2285    2409 
   Size:       1644     252     389     124

  Fragments arranged by size: 

                1644     389     252     124
 
PpuMI rG'GwC_Cy

Cuts at:      0     489    1797    2409 
   Size:        489    1308     612

  Fragments arranged by size: 

                1308     612     489
 
PshAI GACnn'nnGTC

Cuts at:      0    2020    2077    2409 
   Size:       2020      57     332

  Fragments arranged by size: 

                2020     332      57
 
PspOMI G'GGCC_C

Cuts at:      0    1414    2409 
   Size:       1414     995
 
PstI C_TGCA'G

Cuts at:      0     136     624    1553    2058    2309    2409 
   Size:        136     488     929     505     251     100

  Fragments arranged by size: 

                 929     505     488     251     136     100
 
PvuII CAG'CTG

Cuts at:      0      39      57     742     768    1227    2409 
   Size:         39      18     685      26     459    1182

  Fragments arranged by size: 

                1182     685     459      39      26      18
 
SacI G_AGCT'C

Cuts at:      0     590     652     839    2409 
   Size:        590      62     187    1570

  Fragments arranged by size: 

                1570     590     187      62
 
SapI GCTCTTCn'nnn_

Cuts at:      0     160    1099    1198    2409 
   Size:        160     939      99    1211

  Fragments arranged by size: 

                1211     939     160      99
 
ScaI AGT'ACT

Cuts at:      0     142    2409 
   Size:        142    2267
 
SexAI A'CCwGG_T

Cuts at:      0    1367    2409 
   Size:       1367    1042
 
SfcI C'TryA_G

Cuts at:      0     132     620    1549    2054    2305    2378    2409 
   Size:        132     488     929     505     251      73      31

  Fragments arranged by size: 

                 929     505     488     251     132      73      31
 
SmaI CCC'GGG

Cuts at:      0     568     853    2409 
   Size:        568     285    1556

  Fragments arranged by size: 

                1556     568     285
 
SmlI C'TyrA_G

Cuts at:      0     331     493     589    1213    1420    2409 
   Size:        331     162      96     624     207     989

  Fragments arranged by size: 

                 989     624     331     207     162      96
 
StuI AGG'CCT

Cuts at:      0     206     379     898    1024    1612    1780    2409 
   Size:        206     173     519     126     588     168     629

  Fragments arranged by size: 

                 629     588     519     206     173     168     126
 
StyI C'CwwG_G

Cuts at:      0     380     893    2409 
   Size:        380     513    1516

  Fragments arranged by size: 

                1516     513     380
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     486    1633    2409 
   Size:        486    1147     776

  Fragments arranged by size: 

                1147     776     486
 
TatI w'GTAC_w

Cuts at:      0     140     230    2409 
   Size:        140      90    2179

  Fragments arranged by size: 

                2179     140      90
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1324    1356    2042    2074    2409 
   Size:       1324      32     686      32     335

  Fragments arranged by size: 

                1324     686     335      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1984    2409 
   Size:       1984     425
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     609    2282    2409 
   Size:        609    1673     127

  Fragments arranged by size: 

                1673     609     127
 
XmaI C'CCGG_G

Cuts at:      0     566     851    2409 
   Size:        566     285    1558

  Fragments arranged by size: 

                1558     566     285
 
XmnI GAAnn'nnTTC

Cuts at:      0     940     997    1444    2409 
   Size:        940      57     447     965

  Fragments arranged by size: 

                 965     940     447      57
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AleI    AlwNI     ApaI
    ApaLI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
     BglI     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII     MmeI     MscI     MslI   MspA1I     NaeI
   NgoMIV     NotI     NruI     NspI     PasI    PflMI     PfoI     PmlI
    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI     SapI     ScaI
    SexAI     SfcI     SmaI     SmlI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AlfI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BciVI
     BclI    BfrBI    BglII     BmrI     BmtI    BsaHI    BsaWI    BsiWI
    BspEI    BspHI    BsrDI    BstBI   BstEII  BstZ17I     ClaI    CspCI
     DraI   DraIII     DrdI    EcoRI    EcoRV     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NcoI     NdeI     NheI     NsiI     PacI     PciI     PmeI
     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     XbaI     XhoI     ZraI
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