Restriction map for
pFN21AB0408
(Linear) (Six-base) MAPSORT of: KIBB0408.seq Check: 6184 from: 1 to: 2409
KIBB0408.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 204 2289 2409
Size: 204 2085 120
Fragments arranged by size:
2085 204 120
AccI GT'mk_AC
Cuts at: 0 1877 2409
Size: 1877 532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1402 1414 1486 2104 2409
Size: 1096 306 12 72 618 305
Fragments arranged by size:
1096 618 306 305 72 12
AfeI AGC'GCT
Cuts at: 0 1684 2409
Size: 1684 725
AflIII A'CryG_T
Cuts at: 0 1505 2409
Size: 1505 904
AleI CACnn'nnGTG
Cuts at: 0 82 1574 1758 2038 2409
Size: 82 1492 184 280 371
Fragments arranged by size:
1492 371 280 184 82
AlwNI CAG_nnn'CTG
Cuts at: 0 219 628 2229 2304 2386 2409
Size: 219 409 1601 75 82 23
Fragments arranged by size:
1601 409 219 82 75 23
ApaI G_GGCC'C
Cuts at: 0 1418 2409
Size: 1418 991
ApaLI G'TGCA_C
Cuts at: 0 178 1231 1906 2409
Size: 178 1053 675 503
Fragments arranged by size:
1053 675 503 178
AvaI C'yCGr_G
Cuts at: 0 566 787 825 851 1152 1203 1942 2409
Size: 566 221 38 26 301 51 739 467
Fragments arranged by size:
739 566 467 301 221 51 38 26
BamHI G'GATC_C
Cuts at: 0 1481 2409
Size: 1481 928
BanI G'GyrC_C
Cuts at: 0 868 1472 2040 2409
Size: 868 604 568 369
Fragments arranged by size:
868 604 568 369
BanII G_rGCy'C
Cuts at: 0 256 590 652 677 839 1418 1943 2409
Size: 256 334 62 25 162 579 525 466
Fragments arranged by size:
579 525 466 334 256 162 62 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1066 1906 2132 2316 2409
Size: 1066 840 226 184 93
Fragments arranged by size:
1066 840 226 184 93
BbvCI CC'TCA_GC
Cuts at: 0 629 743 2345 2409
Size: 629 114 1602 64
Fragments arranged by size:
1602 629 114 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1680 1714 1764 1798 2409
Size: 1680 34 50 34 611
Fragments arranged by size:
1680 611 50 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2295 2409
Size: 2295 114
BlpI GC'TnA_GC
Cuts at: 0 244 344 439 2198 2409
Size: 244 100 95 1759 211
Fragments arranged by size:
1759 244 211 100 95
Bme1580I G_kGCm'C
Cuts at: 0 182 820 1235 1345 1418 1910 2409
Size: 182 638 415 110 73 492 499
Fragments arranged by size:
638 499 492 415 182 110 73
BmgBI CAC'GTC
Cuts at: 0 174 1560 2409
Size: 174 1386 849
Fragments arranged by size:
1386 849 174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 858 2409
Size: 858 1551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 469 790 1330 1847 1957 2409
Size: 241 228 321 540 517 110 452
Fragments arranged by size:
540 517 452 321 241 228 110
Bpu10I CC'TnA_GC
Cuts at: 0 250 629 743 864 2345 2409
Size: 250 379 114 121 1481 64
Fragments arranged by size:
1481 379 250 121 114 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 478 574 1198 1405 2409
Size: 316 162 96 624 207 1004
Fragments arranged by size:
1004 624 316 207 162 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 1306 2362 2409
Size: 1306 1056 47
Fragments arranged by size:
1306 1056 47
BsaAI yAC'GTr
Cuts at: 0 1506 1644 1896 2285 2409
Size: 1506 138 252 389 124
Fragments arranged by size:
1506 389 252 138 124
BsaBI GATnn'nnATC
Cuts at: 0 1402 2409
Size: 1402 1007
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 1324 1354 1873 1903 2042 2072
Size: 214 30 1080 30 519 30 139 30
Cuts at: 2072 2409
Size: 337
Fragments arranged by size:
1080 519 337 214 139 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 430 514 709 727 736 739 769 805
Size: 430 84 195 18 9 3 30 36
Cuts at: 805 820 1108 1960 2409
Size: 15 288 852 449
Fragments arranged by size:
852 449 430 288 195 84 36 30
18 15 9 3
BseYI C'CCAG_C
Cuts at: 0 11 57 481 764 2311 2409
Size: 11 46 424 283 1547 98
Fragments arranged by size:
1547 424 283 98 46 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 557 1035 1120 1782 1791 2137 2409
Size: 60 497 478 85 662 9 346 272
Fragments arranged by size:
662 497 478 346 272 85 60 9
BsiEI CG_ry'CG
Cuts at: 0 882 1040 1272 2409
Size: 882 158 232 1137
Fragments arranged by size:
1137 882 232 158
BsiHKAI G_wGCw'C
Cuts at: 0 182 590 652 839 1235 1337 1910 2409
Size: 182 408 62 187 396 102 573 499
Fragments arranged by size:
573 499 408 396 187 182 102 62
BsmI GAATG_Cn'
Cuts at: 0 1565 2409
Size: 1565 844
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2065 2409
Size: 2065 344
Bsp1286I G_dGCh'C
Cuts at: 0 182 256 590 652 677 820 839 1235
Size: 182 74 334 62 25 143 19 396
Cuts at: 1235 1337 1345 1418 1910 1943 2409
Size: 102 8 73 492 33 466
Fragments arranged by size:
492 466 396 334 182 143 102 74
73 62 33 25 19 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 204 1142 1892 2289 2409
Size: 204 938 750 397 120
Fragments arranged by size:
938 750 397 204 120
BsrBI CCG'CTC
Cuts at: 0 1274 2409
Size: 1274 1135
BsrFI r'CCGG_y
Cuts at: 0 348 2043 2409
Size: 348 1695 366
Fragments arranged by size:
1695 366 348
BsrGI T'GTAC_A
Cuts at: 0 230 2409
Size: 230 2179
BssHII G'CGCG_C
Cuts at: 0 1162 2409
Size: 1162 1247
BssSI C'ACGA_G
Cuts at: 0 603 1002 2409
Size: 603 399 1407
Fragments arranged by size:
1407 603 399
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1517 1769 2304 2409
Size: 1517 252 535 105
Fragments arranged by size:
1517 535 252 105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1716 2193 2409
Size: 1716 477 216
Fragments arranged by size:
1716 477 216
BstYI r'GATC_y
Cuts at: 0 1397 1481 1931 2409
Size: 1397 84 450 478
Fragments arranged by size:
1397 478 450 84
Bsu36I CC'TnA_GG
Cuts at: 0 1455 2409
Size: 1455 954
BtgI C'CryG_G
Cuts at: 0 281 2409
Size: 281 2128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1389 1620 2409
Size: 1389 231 789
Fragments arranged by size:
1389 789 231
BtsI GCAGTG_nn'
Cuts at: 0 125 1347 1515 1959 2063 2409
Size: 125 1222 168 444 104 346
Fragments arranged by size:
1222 444 346 168 125 104
EaeI y'GGCC_r
Cuts at: 0 236 278 284 383 425 527 1037 1269
Size: 236 42 6 99 42 102 510 232
Cuts at: 1269 1718 2409
Size: 449 691
Fragments arranged by size:
691 510 449 236 232 102 99 42
42 6
EagI C'GGCC_G
Cuts at: 0 1037 1269 2409
Size: 1037 232 1140
Fragments arranged by size:
1140 1037 232
EarI CTCTTCn'nnn_
Cuts at: 0 160 686 1099 1198 2409
Size: 160 526 413 99 1211
Fragments arranged by size:
1211 526 413 160 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1738 2317 2409
Size: 1738 579 92
Fragments arranged by size:
1738 579 92
EcoICRI GAG'CTC
Cuts at: 0 588 650 837 2409
Size: 588 62 187 1572
Fragments arranged by size:
1572 588 187 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 469 790 1096 1330 1402 1414 1486
Size: 241 228 321 306 234 72 12 72
Cuts at: 1486 1847 1957 2104 2409
Size: 361 110 147 305
Fragments arranged by size:
361 321 306 305 241 234 228 147
110 72 72 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 211 496 2409
Size: 211 285 1913
Fragments arranged by size:
1913 285 211
EcoO109I rG'GnC_Cy
Cuts at: 0 478 489 783 855 1415 1797 2409
Size: 478 11 294 72 560 382 612
Fragments arranged by size:
612 560 478 382 294 72 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 171 2409
Size: 171 2238
HaeII r_GCGC'y
Cuts at: 0 1221 1547 1686 2409
Size: 1221 326 139 723
Fragments arranged by size:
1221 723 326 139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1055 1307 2409
Size: 1055 252 1102
Fragments arranged by size:
1102 1055 252
MscI TGG'CCA
Cuts at: 0 286 385 427 1720 2409
Size: 286 99 42 1293 689
Fragments arranged by size:
1293 689 286 99 42
MslI CAynn'nnrTG
Cuts at: 0 82 1574 1590 1758 2038 2409
Size: 82 1492 16 168 280 371
Fragments arranged by size:
1492 371 280 168 82 16
MspA1I CmG'CkG
Cuts at: 0 39 51 57 533 742 768 814 1172
Size: 39 12 6 476 209 26 46 358
Cuts at: 1172 1227 1238 1490 1554 2409
Size: 55 11 252 64 855
Fragments arranged by size:
855 476 358 252 209 64 55 46
39 26 12 11 6
NaeI GCC'GGC
Cuts at: 0 350 2409
Size: 350 2059
NgoMIV G'CCGG_C
Cuts at: 0 348 2409
Size: 348 2061
NotI GC'GGCC_GC
Cuts at: 0 1269 2409
Size: 1269 1140
NruI TCG'CGA
Cuts at: 0 2336 2409
Size: 2336 73
NspI r_CATG'y
Cuts at: 0 28 2206 2409
Size: 28 2178 203
Fragments arranged by size:
2178 203 28
PasI CC'CwG_GG
Cuts at: 0 361 2409
Size: 361 2048
PflMI CCAn_nnn'nTGG
Cuts at: 0 1227 2229 2409
Size: 1227 1002 180
Fragments arranged by size:
1227 1002 180
PfoI T'CCnGG_A
Cuts at: 0 1934 2409
Size: 1934 475
PmlI CAC'GTG
Cuts at: 0 1644 1896 2285 2409
Size: 1644 252 389 124
Fragments arranged by size:
1644 389 252 124
PpuMI rG'GwC_Cy
Cuts at: 0 489 1797 2409
Size: 489 1308 612
Fragments arranged by size:
1308 612 489
PshAI GACnn'nnGTC
Cuts at: 0 2020 2077 2409
Size: 2020 57 332
Fragments arranged by size:
2020 332 57
PspOMI G'GGCC_C
Cuts at: 0 1414 2409
Size: 1414 995
PstI C_TGCA'G
Cuts at: 0 136 624 1553 2058 2309 2409
Size: 136 488 929 505 251 100
Fragments arranged by size:
929 505 488 251 136 100
PvuII CAG'CTG
Cuts at: 0 39 57 742 768 1227 2409
Size: 39 18 685 26 459 1182
Fragments arranged by size:
1182 685 459 39 26 18
SacI G_AGCT'C
Cuts at: 0 590 652 839 2409
Size: 590 62 187 1570
Fragments arranged by size:
1570 590 187 62
SapI GCTCTTCn'nnn_
Cuts at: 0 160 1099 1198 2409
Size: 160 939 99 1211
Fragments arranged by size:
1211 939 160 99
ScaI AGT'ACT
Cuts at: 0 142 2409
Size: 142 2267
SexAI A'CCwGG_T
Cuts at: 0 1367 2409
Size: 1367 1042
SfcI C'TryA_G
Cuts at: 0 132 620 1549 2054 2305 2378 2409
Size: 132 488 929 505 251 73 31
Fragments arranged by size:
929 505 488 251 132 73 31
SmaI CCC'GGG
Cuts at: 0 568 853 2409
Size: 568 285 1556
Fragments arranged by size:
1556 568 285
SmlI C'TyrA_G
Cuts at: 0 331 493 589 1213 1420 2409
Size: 331 162 96 624 207 989
Fragments arranged by size:
989 624 331 207 162 96
StuI AGG'CCT
Cuts at: 0 206 379 898 1024 1612 1780 2409
Size: 206 173 519 126 588 168 629
Fragments arranged by size:
629 588 519 206 173 168 126
StyI C'CwwG_G
Cuts at: 0 380 893 2409
Size: 380 513 1516
Fragments arranged by size:
1516 513 380
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 486 1633 2409
Size: 486 1147 776
Fragments arranged by size:
1147 776 486
TatI w'GTAC_w
Cuts at: 0 140 230 2409
Size: 140 90 2179
Fragments arranged by size:
2179 140 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1324 1356 2042 2074 2409
Size: 1324 32 686 32 335
Fragments arranged by size:
1324 686 335 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1984 2409
Size: 1984 425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 609 2282 2409
Size: 609 1673 127
Fragments arranged by size:
1673 609 127
XmaI C'CCGG_G
Cuts at: 0 566 851 2409
Size: 566 285 1558
Fragments arranged by size:
1558 566 285
XmnI GAAnn'nnTTC
Cuts at: 0 940 997 1444 2409
Size: 940 57 447 965
Fragments arranged by size:
965 940 447 57
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII MmeI MscI MslI MspA1I NaeI
NgoMIV NotI NruI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII SacI SapI ScaI
SexAI SfcI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI
BclI BfrBI BglII BmrI BmtI BsaHI BsaWI BsiWI
BspEI BspHI BsrDI BstBI BstEII BstZ17I ClaI CspCI
DraI DraIII DrdI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NsiI PacI PciI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XhoI ZraI