(Circular) (Six-base) MAPSORT of: pFN21AB6687.seq Check: 5080 from: 1 to: 5986 pFN21AB6687 5986 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 2619 4113 4486 1563 Size: 1056 1494 373 3063 Fragments arranged by size: 3063 1494 1056 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3009 4049 5433 5701 1632 Size: 1377 1040 1384 268 1917 Fragments arranged by size: 1917 1384 1377 1040 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 4885 5862 1203 Size: 3682 977 1327 Fragments arranged by size: 3682 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 2629 4715 2629 Size: 2086 3900 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2365 1184 Size: 1181 4805 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2722 2754 2722 Size: 32 5954 AlwNI CAG_nnn'CTG Cuts at: 2410 3263 3729 5301 2410 Size: 853 466 1572 3095 Fragments arranged by size: 3095 1572 853 466 ApaLI G'TGCA_C Cuts at: 4043 5199 4043 Size: 1156 4830 ApoI r'AATT_y Cuts at: 1493 2086 2303 2744 3295 3521 3673 5782 1493 Size: 593 217 441 551 226 152 2109 1697 Fragments arranged by size: 2109 1697 593 551 441 226 217 152 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 2585 3311 1870 Size: 73 642 726 4545 Fragments arranged by size: 4545 726 642 73 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3307 4762 618 Size: 325 217 627 1520 1455 1842 Fragments arranged by size: 1842 1520 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2113 2334 3305 1391 Size: 722 221 971 4072 Fragments arranged by size: 4072 971 722 221 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3195 928 Size: 971 1296 3719 Fragments arranged by size: 3719 1296 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4177 4211 1903 Size: 34 2240 34 3678 Fragments arranged by size: 3678 2240 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 2658 2698 3153 1548 Size: 1110 40 455 4381 Fragments arranged by size: 4381 1110 455 40 BfrBI ATG'CAT Cuts at: 2921 2921 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2210 2882 4597 Size: 107 122 71 631 605 538 672 1715 Cuts at: 4597 136 Size: 1525 Fragments arranged by size: 1715 1525 672 631 605 538 122 107 71 BglII A'GATC_T Cuts at: 5981 5981 Size: 5986 BlpI GC'TnA_GC Cuts at: 2174 3403 2174 Size: 1229 4757 Bme1580I G_kGCm'C Cuts at: 1790 4047 5203 1790 Size: 2257 1156 2573 Fragments arranged by size: 2573 2257 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2460 3717 4675 Size: 416 53 83 399 24 1043 1257 958 Cuts at: 4675 442 Size: 1753 Fragments arranged by size: 1753 1257 1043 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4646 1239 Size: 3407 2579 Bpu10I CC'TnA_GC Cuts at: 2104 2104 Size: 5986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2097 4111 4976 5274 2097 Size: 2014 865 298 2809 Fragments arranged by size: 2809 2014 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4649 882 Size: 617 3150 2219 Fragments arranged by size: 3150 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3777 493 Size: 711 2573 2702 Fragments arranged by size: 2702 2573 711 BsaBI GATnn'nnATC Cuts at: 2343 5980 2343 Size: 3637 2349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2552 3793 4175 4810 Size: 53 83 186 936 1019 1241 382 635 Cuts at: 4810 275 Size: 1451 Fragments arranged by size: 1451 1241 1019 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4419 4817 5091 5238 5649 4419 Size: 398 274 147 411 4756 Fragments arranged by size: 4756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5761 Fragments arranged by size: 5761 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3110 1186 Size: 1924 4062 BseYI C'CCAG_C Cuts at: 2845 3263 5189 5685 2845 Size: 418 1926 496 3146 Fragments arranged by size: 3146 1926 496 418 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3165 1163 Size: 573 1429 3984 Fragments arranged by size: 3984 1429 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3436 4197 5225 1540 Size: 275 186 1435 761 1028 2301 Fragments arranged by size: 2301 1435 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2113 2334 2584 4047 4132 5203 2113 Size: 221 250 1463 85 1071 2896 Fragments arranged by size: 2896 1463 1071 250 221 85 BsmI GAATG_Cn' Cuts at: 3497 3590 3497 Size: 93 5893 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2113 2334 2584 3305 4047 4132 5203 Size: 399 323 221 250 721 742 85 1071 Cuts at: 5203 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 721 399 323 250 221 85 BspEI T'CCGG_A Cuts at: 5649 5649 Size: 5986 BspHI T'CATG_A Cuts at: 2377 2748 3875 5605 2377 Size: 371 1127 1730 2758 Fragments arranged by size: 2758 1730 1127 371 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3341 844 Size: 2497 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4481 4655 65 Size: 1183 3233 174 1396 Fragments arranged by size: 3233 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4630 4817 1700 Size: 110 2820 187 2869 Fragments arranged by size: 2869 2820 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5986 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstXI CCAn_nnnn'nTGG Cuts at: 2908 2908 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2931 3012 3217 3316 3683 4068 4085 5537 Size: 959 81 205 99 367 385 17 1452 Cuts at: 5537 5623 5635 5981 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 959 385 367 346 205 99 86 81 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5434 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 3156 3502 4296 4316 3156 Size: 346 794 20 4826 Fragments arranged by size: 4826 794 346 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 2091 3290 2091 Size: 1199 4787 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2520 3433 4322 Size: 54 1425 50 275 18 690 913 889 Cuts at: 4322 5951 8 Size: 1629 43 Fragments arranged by size: 1629 1425 913 889 690 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2831 3048 3916 1353 Size: 1478 217 868 3423 Fragments arranged by size: 3423 1478 868 217 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4573 4947 5093 387 Size: 1064 138 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2111 2332 2111 Size: 221 5765 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3009 4049 4646 5433 5701 1239 Size: 393 1377 1040 597 787 268 1524 Fragments arranged by size: 1524 1377 1040 787 597 393 268 EcoRI G'AATT_C Cuts at: 2744 3295 3673 2744 Size: 551 378 5057 Fragments arranged by size: 5057 551 378 EcoRV GAT'ATC Cuts at: 2081 2081 Size: 5986 FspI TGC'GCA Cuts at: 2128 4492 2128 Size: 2364 3622 HaeII r_GCGC'y Cuts at: 1678 5133 1678 Size: 3455 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2615 2643 2647 2675 2882 Size: 32 135 32 1043 28 4 28 207 Cuts at: 2882 2914 4627 4659 4701 4733 1373 Size: 32 1713 32 42 32 2626 Fragments arranged by size: 2626 1713 1043 207 135 42 32 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 2623 3213 3330 3576 669 Size: 988 281 685 590 117 246 3079 Fragments arranged by size: 3079 988 685 590 281 246 117 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2977 3245 5100 5284 1305 Size: 1672 268 1855 184 2007 Fragments arranged by size: 2007 1855 1672 268 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2522 10 Size: 54 1425 343 690 3474 Fragments arranged by size: 3474 1425 690 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2324 3944 4303 4462 518 Size: 648 1158 1620 359 159 2042 Fragments arranged by size: 2042 1620 1158 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2849 3263 3402 4079 5227 5472 1606 Size: 1243 414 139 677 1148 245 2120 Fragments arranged by size: 2120 1243 1148 677 414 245 139 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5434 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NsiI A_TGCA'T Cuts at: 2923 2923 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 3344 4889 1132 Size: 97 2115 1545 2229 Fragments arranged by size: 2229 2115 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5986 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5986 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2211 2243 4055 4087 5568 5600 2211 Size: 32 1812 32 1481 32 2597 Fragments arranged by size: 2597 1812 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2514 3246 1613 Size: 901 732 4353 Fragments arranged by size: 4353 901 732 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PstI C_TGCA'G Cuts at: 830 2533 3338 830 Size: 1703 805 3478 Fragments arranged by size: 3478 1703 805 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 PvuII CAG'CTG Cuts at: 2849 3263 2849 Size: 414 5572 SacI G_AGCT'C Cuts at: 2113 2334 2113 Size: 221 5765 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SfcI C'TryA_G Cuts at: 826 1045 2097 2215 2529 2575 2937 3280 3334 Size: 219 1052 118 314 46 362 343 54 Cuts at: 3334 4469 5150 5341 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1052 681 362 343 314 219 191 118 54 46 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 3313 1872 Size: 1441 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2112 4090 4991 5253 820 Size: 197 926 169 1978 901 262 1553 Fragments arranged by size: 1978 1553 926 901 262 197 169 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5986 StyI C'CwwG_G Cuts at: 513 1065 2596 3425 513 Size: 552 1531 829 3074 Fragments arranged by size: 3074 1531 829 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2504 2628 4013 4030 4183 4368 4813 Size: 1255 924 124 1385 17 153 185 445 Cuts at: 4813 325 Size: 1498 Fragments arranged by size: 1498 1385 1255 924 445 185 153 124 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4232 5944 96 Size: 274 80 33 51 1443 2255 1712 138 Fragments arranged by size: 2255 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2997 3029 5946 5978 2997 Size: 32 2917 32 3005 Fragments arranged by size: 3005 2917 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5986 XbaI T'CTAG_A Cuts at: 3250 3322 3250 Size: 72 5914 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 3311 1870 Size: 1441 4545 XmnI GAAnn'nnTTC Cuts at: 2276 2304 2825 4115 2276 Size: 28 521 1290 4147 Fragments arranged by size: 4147 1290 521 28 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI