(Circular) (Six-base) MAPSORT of: pFN21AB6679.seq Check: 8063 from: 1 to: 5986 pFN21AB6679 5986 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2510 4049 5433 5701 1632 Size: 878 1539 1384 268 1917 Fragments arranged by size: 1917 1539 1384 878 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 3123 4885 5862 1203 Size: 1920 1762 977 1327 Fragments arranged by size: 1920 1762 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AlwNI CAG_nnn'CTG Cuts at: 2191 3729 5301 2191 Size: 1538 1572 2876 Fragments arranged by size: 2876 1572 1538 ApaLI G'TGCA_C Cuts at: 4043 5199 4043 Size: 1156 4830 ApoI r'AATT_y Cuts at: 1493 2422 2808 3200 3295 3521 3673 5782 1493 Size: 929 386 392 95 226 152 2109 1697 Fragments arranged by size: 2109 1697 929 392 386 226 152 95 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 2099 3311 1870 Size: 73 156 1212 4545 Fragments arranged by size: 4545 1212 156 73 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2361 3307 4762 618 Size: 325 217 627 574 946 1455 1842 Fragments arranged by size: 1842 1455 946 627 574 325 217 BanII G_rGCy'C Cuts at: 1391 2100 2129 2274 2908 3305 1391 Size: 709 29 145 634 397 4072 Fragments arranged by size: 4072 709 634 397 145 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2042 928 Size: 971 143 4872 Fragments arranged by size: 4872 971 143 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4177 4211 1903 Size: 34 2240 34 3678 Fragments arranged by size: 3678 2240 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2866 2976 4597 Size: 107 122 71 631 605 1194 110 1621 Cuts at: 4597 136 Size: 1525 Fragments arranged by size: 1621 1525 1194 631 605 122 110 107 71 BglII A'GATC_T Cuts at: 5981 5981 Size: 5986 BlpI GC'TnA_GC Cuts at: 3403 3403 Size: 5986 Bme1580I G_kGCm'C Cuts at: 1790 4047 5203 1790 Size: 2257 1156 2573 Fragments arranged by size: 2573 2257 1156 BmgBI CAC'GTC Cuts at: 1318 3124 1318 Size: 1806 4180 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2493 2953 3717 Size: 416 53 83 399 24 1076 460 764 Cuts at: 3717 4675 442 Size: 958 1753 Fragments arranged by size: 1753 1076 958 764 460 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2927 2927 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2114 2258 2490 3277 4646 1239 Size: 869 6 144 232 787 1369 2579 Fragments arranged by size: 2579 1369 869 787 232 144 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4111 4976 5274 4111 Size: 865 298 4823 Fragments arranged by size: 4823 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4649 882 Size: 617 3150 2219 Fragments arranged by size: 3150 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3777 493 Size: 711 2573 2702 Fragments arranged by size: 2702 2573 711 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3793 4175 4810 275 Size: 53 83 186 936 2260 382 635 1451 Fragments arranged by size: 2260 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2129 2307 4419 4817 5091 5238 5649 2129 Size: 178 2112 398 274 147 411 2466 Fragments arranged by size: 2466 2112 411 398 274 178 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2065 2095 2652 2682 1178 Size: 30 165 30 662 30 557 30 4482 Fragments arranged by size: 4482 662 557 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2086 2518 1186 Size: 900 432 4654 Fragments arranged by size: 4654 900 432 BseYI C'CCAG_C Cuts at: 2236 2375 5189 5685 2236 Size: 139 2814 496 2537 Fragments arranged by size: 2814 2537 496 139 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5413 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3120 3436 4197 5225 1540 Size: 275 186 1119 316 761 1028 2301 Fragments arranged by size: 2301 1119 1028 761 316 275 186 BsiHKAI G_wGCw'C Cuts at: 4047 4132 5203 4047 Size: 85 1071 4830 Fragments arranged by size: 4830 1071 85 BsmI GAATG_Cn' Cuts at: 2727 3497 3590 2727 Size: 770 93 5123 Fragments arranged by size: 5123 770 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2129 2274 2908 3305 4047 4132 Size: 399 310 29 145 634 397 742 85 Cuts at: 4132 5203 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 634 399 397 310 145 85 29 BspEI T'CCGG_A Cuts at: 5649 5649 Size: 5986 BspHI T'CATG_A Cuts at: 3875 5605 3875 Size: 1730 4256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2826 3341 844 Size: 1982 515 3489 Fragments arranged by size: 3489 1982 515 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3240 4481 4655 65 Size: 1183 1992 1241 174 1396 Fragments arranged by size: 1992 1396 1241 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4630 4817 1700 Size: 110 2820 187 2869 Fragments arranged by size: 2869 2820 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5986 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 3239 1184 Size: 2055 3931 BstXI CCAn_nnnn'nTGG Cuts at: 2383 2383 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2187 2937 3076 3316 3683 4068 4085 5537 Size: 215 750 139 240 367 385 17 1452 Cuts at: 5537 5623 5635 5981 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 750 385 367 346 240 215 139 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2382 2919 3210 513 Size: 552 939 378 537 291 3289 Fragments arranged by size: 3289 939 552 537 378 291 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 3502 4296 4316 3502 Size: 794 20 5172 Fragments arranged by size: 5172 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 2563 3290 2563 Size: 727 5259 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2252 2778 3433 Size: 54 1425 50 275 18 422 526 655 Cuts at: 3433 4322 5951 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 655 526 422 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2924 3916 1353 Size: 1571 992 3423 Fragments arranged by size: 3423 1571 992 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4573 4947 5093 387 Size: 1064 138 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2114 2258 2490 2510 3277 4049 Size: 393 476 6 144 232 20 767 772 Cuts at: 4049 4646 5433 5701 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 772 767 597 476 393 268 232 144 20 6 EcoNI CCTnn'n_nnAGG Cuts at: 2335 2822 2335 Size: 487 5499 EcoO109I rG'GnC_Cy Cuts at: 2217 2815 2217 Size: 598 5388 EcoRI G'AATT_C Cuts at: 3200 3295 3673 3200 Size: 95 378 5513 Fragments arranged by size: 5513 378 95 EcoRV GAT'ATC Cuts at: 2410 2410 Size: 5986 FspI TGC'GCA Cuts at: 4492 4492 Size: 5986 HaeII r_GCGC'y Cuts at: 1678 5133 1678 Size: 3455 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2065 2097 2463 2495 3122 Size: 32 135 32 493 32 366 32 627 Cuts at: 3122 3139 3154 3158 3171 3190 4627 4659 4701 Size: 17 15 4 13 19 1437 32 42 Cuts at: 4701 4733 1373 Size: 32 2626 Fragments arranged by size: 2626 1437 627 493 366 135 42 32 32 32 32 32 32 19 17 15 13 4 HincII GTy'rAC Cuts at: 669 1657 1938 2878 3330 3576 669 Size: 988 281 940 452 246 3079 Fragments arranged by size: 3079 988 940 452 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5100 5284 1305 Size: 3795 184 2007 Fragments arranged by size: 3795 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2254 2780 10 Size: 54 1425 343 422 526 3216 Fragments arranged by size: 3216 1425 526 422 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3059 3944 4303 4462 518 Size: 648 1893 885 359 159 2042 Fragments arranged by size: 2042 1893 885 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3402 4079 5227 5472 1606 Size: 1796 677 1148 245 2120 Fragments arranged by size: 2120 1796 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3210 513 Size: 552 939 1206 3289 Fragments arranged by size: 3289 1206 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 2747 3344 4889 1132 Size: 97 1518 597 1545 2229 Fragments arranged by size: 2229 1545 1518 597 97 PasI CC'CwG_GG Cuts at: 1348 2974 1348 Size: 1626 4360 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5986 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2217 2815 2217 Size: 598 5388 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PstI C_TGCA'G Cuts at: 830 3338 830 Size: 2508 3478 PvuI CG_AT'CG Cuts at: 2001 3120 2001 Size: 1119 4867 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SapI GCTCTTCn'nnn_ Cuts at: 2924 2924 Size: 5986 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SfcI C'TryA_G Cuts at: 826 1045 3334 4469 5150 5341 826 Size: 219 2289 1135 681 191 1471 Fragments arranged by size: 2289 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 3313 1872 Size: 1441 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4090 4991 5253 820 Size: 197 926 2147 901 262 1553 Fragments arranged by size: 2147 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5986 StyI C'CwwG_G Cuts at: 513 1065 2004 2352 3210 3425 513 Size: 552 939 348 858 215 3074 Fragments arranged by size: 3074 939 858 552 348 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2155 2379 3034 4013 4030 4183 4368 Size: 1255 575 224 655 979 17 153 185 Cuts at: 4368 4813 325 Size: 445 1498 Fragments arranged by size: 1498 1255 979 655 575 445 224 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4232 5944 96 Size: 274 80 33 51 1443 2255 1712 138 Fragments arranged by size: 2255 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2652 2684 5946 5978 2652 Size: 32 3262 32 2660 Fragments arranged by size: 3262 2660 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5986 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2766 2766 Size: 5986 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 3311 1870 Size: 1441 4545 XmnI GAAnn'nnTTC Cuts at: 4115 4115 Size: 5986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI