(Circular) (Six-base) MAPSORT of: pFN21AB6677.seq Check: 3044 from: 1 to: 5986 pFN21AB6677 5986 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 2683 3307 2683 Size: 624 5362 AclI AA'CG_TT Cuts at: 1563 3285 4113 4486 1563 Size: 1722 828 373 3063 Fragments arranged by size: 3063 1722 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2217 2682 4049 5433 5701 1632 Size: 585 465 1367 1384 268 1917 Fragments arranged by size: 1917 1384 1367 585 465 268 AflII C'TTAA_G Cuts at: 820 1017 2897 820 Size: 197 1880 3909 Fragments arranged by size: 3909 1880 197 AflIII A'CryG_T Cuts at: 1203 4885 5862 1203 Size: 3682 977 1327 Fragments arranged by size: 3682 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AlwNI CAG_nnn'CTG Cuts at: 3729 5301 3729 Size: 1572 4414 ApaLI G'TGCA_C Cuts at: 2812 4043 5199 2812 Size: 1231 1156 3599 Fragments arranged by size: 3599 1231 1156 ApoI r'AATT_y Cuts at: 1493 2741 3085 3128 3295 3521 3673 5782 1493 Size: 1248 344 43 167 226 152 2109 1697 Fragments arranged by size: 2109 1697 1248 344 226 167 152 43 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 3311 1870 Size: 73 1368 4545 Fragments arranged by size: 4545 1368 73 BamHI G'GATC_C Cuts at: 2634 3316 3683 2634 Size: 682 367 4937 Fragments arranged by size: 4937 682 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2683 3307 4762 618 Size: 325 217 627 896 624 1455 1842 Fragments arranged by size: 1842 1455 896 627 624 325 217 BanII G_rGCy'C Cuts at: 1391 2139 3305 1391 Size: 748 1166 4072 Fragments arranged by size: 4072 1166 748 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5015 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4177 4211 1903 Size: 34 2240 34 3678 Fragments arranged by size: 3678 2240 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 2449 1548 Size: 901 5085 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4597 136 Size: 107 122 71 631 605 2925 1525 Fragments arranged by size: 2925 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5981 5981 Size: 5986 BlpI GC'TnA_GC Cuts at: 2138 3403 2138 Size: 1265 4721 Bme1580I G_kGCm'C Cuts at: 1790 2816 4047 5203 1790 Size: 1026 1231 1156 2573 Fragments arranged by size: 2573 1231 1156 1026 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3717 4675 442 Size: 416 53 83 399 24 2300 958 1753 Fragments arranged by size: 2300 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2593 4646 1239 Size: 1354 2053 2579 Fragments arranged by size: 2579 2053 1354 Bpu10I CC'TnA_GC Cuts at: 2309 2309 Size: 5986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2893 4111 4976 5274 2893 Size: 1218 865 298 3605 Fragments arranged by size: 3605 1218 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2373 4649 882 Size: 617 874 2276 2219 Fragments arranged by size: 2276 2219 874 617 BsaAI yAC'GTr Cuts at: 493 1204 3777 493 Size: 711 2573 2702 Fragments arranged by size: 2702 2573 711 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3793 4175 4810 275 Size: 53 83 186 936 2260 382 635 1451 Fragments arranged by size: 2260 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3258 4419 4817 5091 5238 5649 3258 Size: 1161 398 274 147 411 3595 Fragments arranged by size: 3595 1161 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5761 Fragments arranged by size: 5761 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5986 BseYI C'CCAG_C Cuts at: 2183 2432 5189 5685 2183 Size: 249 2757 496 2484 Fragments arranged by size: 2757 2484 496 249 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5413 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3436 4197 5225 1540 Size: 275 186 1435 761 1028 2301 Fragments arranged by size: 2301 1435 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2139 2816 4047 4132 5203 2139 Size: 677 1231 85 1071 2922 Fragments arranged by size: 2922 1231 1071 677 85 BsmI GAATG_Cn' Cuts at: 2302 3497 3590 2302 Size: 1195 93 4698 Fragments arranged by size: 4698 1195 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2816 3305 4047 4132 5203 1391 Size: 399 349 677 489 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 677 489 399 349 85 BspEI T'CCGG_A Cuts at: 5649 5649 Size: 5986 BspHI T'CATG_A Cuts at: 2835 3875 5605 2835 Size: 1040 1730 3216 Fragments arranged by size: 3216 1730 1040 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3341 844 Size: 2497 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2194 4481 4655 65 Size: 1183 946 2287 174 1396 Fragments arranged by size: 2287 1396 1183 946 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4630 4817 1700 Size: 110 2820 187 2869 Fragments arranged by size: 2869 2820 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5986 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 2149 1184 Size: 965 5021 BstXI CCAn_nnnn'nTGG Cuts at: 2173 2173 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2634 3316 3683 4068 4085 5537 5623 5635 Size: 662 682 367 385 17 1452 86 12 Cuts at: 5635 5981 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 682 662 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2021 2021 Size: 5986 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4495 Fragments arranged by size: 4495 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 2814 3502 4296 4316 2814 Size: 688 794 20 4484 Fragments arranged by size: 4484 794 688 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3133 3290 3133 Size: 157 5829 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2652 3433 4322 Size: 54 1425 50 275 18 822 781 889 Cuts at: 4322 5951 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 822 781 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2786 2973 3916 1353 Size: 1433 187 943 3423 Fragments arranged by size: 3423 1433 943 187 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4573 4947 5093 387 Size: 1064 138 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2137 2137 Size: 5986 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 2593 2682 4049 4646 5433 5701 Size: 393 585 376 89 1367 597 787 268 Cuts at: 5701 1239 Size: 1524 Fragments arranged by size: 1524 1367 787 597 585 393 376 268 89 EcoRI G'AATT_C Cuts at: 3085 3295 3673 3085 Size: 210 378 5398 Fragments arranged by size: 5398 378 210 EcoRV GAT'ATC Cuts at: 2262 2262 Size: 5986 FspI TGC'GCA Cuts at: 4492 4492 Size: 5986 HaeII r_GCGC'y Cuts at: 1678 2440 5133 1678 Size: 762 2693 2531 Fragments arranged by size: 2693 2531 762 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2043 2075 2350 2370 2382 Size: 32 135 32 471 32 275 20 12 Cuts at: 2382 2402 4627 4659 4701 4733 1373 Size: 20 2225 32 42 32 2626 Fragments arranged by size: 2626 2225 471 275 135 42 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2548 3330 3576 669 Size: 988 281 610 782 246 3079 Fragments arranged by size: 3079 988 782 610 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KpnI G_GTAC'C Cuts at: 2687 3311 2687 Size: 624 5362 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2029 5100 5284 1305 Size: 724 3071 184 2007 Fragments arranged by size: 3071 2007 724 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2654 10 Size: 54 1425 343 822 3342 Fragments arranged by size: 3342 1425 822 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2106 2834 3944 4303 4462 518 Size: 648 940 728 1110 359 159 2042 Fragments arranged by size: 2042 1110 940 728 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3402 4079 5227 5472 1606 Size: 1796 677 1148 245 2120 Fragments arranged by size: 2120 1796 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4495 Fragments arranged by size: 4495 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 3344 4889 1132 Size: 97 2115 1545 2229 Fragments arranged by size: 2229 2115 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5986 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5986 PfoI T'CCnGG_A Cuts at: 2570 2570 Size: 5986 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3194 1613 Size: 1581 4405 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PstI C_TGCA'G Cuts at: 830 3338 830 Size: 2508 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 SacI G_AGCT'C Cuts at: 2139 2139 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SapI GCTCTTCn'nnn_ Cuts at: 2786 2786 Size: 5986 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SfcI C'TryA_G Cuts at: 826 1045 3334 4469 5150 5341 826 Size: 219 2289 1135 681 191 1471 Fragments arranged by size: 2289 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 3313 1872 Size: 1441 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2872 2897 4090 4991 5253 820 Size: 197 926 929 25 1193 901 262 1553 Fragments arranged by size: 1553 1193 929 926 901 262 197 25 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5986 StyI C'CwwG_G Cuts at: 513 1065 2004 2374 2805 3425 513 Size: 552 939 370 431 620 3074 Fragments arranged by size: 3074 939 620 552 431 370 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3184 4013 4030 4183 4368 4813 325 Size: 1255 1604 829 17 153 185 445 1498 Fragments arranged by size: 1604 1498 1255 829 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2267 4232 5944 Size: 274 80 33 51 1443 290 1965 1712 Cuts at: 5944 96 Size: 138 Fragments arranged by size: 1965 1712 1443 290 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5946 5978 5946 Size: 32 5954 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5986 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 3311 1870 Size: 1441 4545 XmnI GAAnn'nnTTC Cuts at: 4115 4115 Size: 5986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI