Restriction map for
pFN21AB6653
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BfrI
BglI BglII BlnI BpmI BsaAI
BsaBI BsaHI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BspDI BspHI
BspLU11I BsrDI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MscI MseI MspA1I
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspI SwaI Tru9I
Tth111I Van91I XbaI XcmI XhoII
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 427 GTAGAC GT^[AC][GT]AC
AccIII 1086 TCCGGA T^CCGGA
AcsI 1123 GAATTT [GA]^AATT[CT]
AgeI 584 ACCGGT A^CCGGT
AlwI 504 GATCC GGATC(4/5)
ApoI 1123 GAATTT [GA]^AATT[CT]
BbvCI 315 GCTGAGG CCTCAGC(-5/-2)
BciVI 405 GGATAC GTATCC(6/5)
BclI 1215 TGATCA T^GATCA
BfaI 463 CTAG C^TAG
BlpI 834 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 834 GCTCAGC GC^T[ACGT]AGC
BsaI 859 GGTCTC GGTCTC(1/5)
BseAI 1086 TCCGGA T^CCGGA
Bsp1407I 815 TGTACA T^GTACA
BspEI 1086 TCCGGA T^CCGGA
BspMI 175 ACCTGC ACCTGC(4/8)
BsrBI 1082 CCGCTC CCGCTC(-3/-3)
BsrGI 815 TGTACA T^GTACA
BssSI 886 CACGAG CACGAG(-5/-1)
CelII 834 GCTCAGC GC^T[ACGT]AGC
CfoI 179 GCGC GCG^C
DsaI 630 CCATGG C^C[GA][CT]GG
EaeI 192 TGGCCG [CT]^GGCC[GA]
EcoNI 609 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 630 CCATGG C^C[AT][AT]GG
FbaI 1215 TGATCA T^GATCA
HhaI 179 GCGC GCG^C
HinP1I 179 GCGC G^CGC
MaeI 463 CTAG C^TAG
MaeII 659 ACGT A^CGT
MroI 1086 TCCGGA T^CCGGA
MslI 912 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 630 CCATGG C^CATGG
NspI 333 GCATGC [GA]CATG^[CT]
PinAI 584 ACCGGT A^CCGGT
SbfI 300 CCTGCAGG CCTGCA^GG
SexAI 782 ACCAGGT A^CC[AT]GGT
SmaI 13 CCCGGG CCC^GGG
SphI 333 GCATGC GCATG^C
Sse8387I 300 CCTGCAGG CCTGCA^GG
SspBI 815 TGTACA T^GTACA
StyI 630 CCATGG C^C[AT][AT]GG
Tsp509I 1124 AATT ^AATT
XmaCI 13 CCCGGG C^CCGGG
XmaI 13 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BanI 270 GGTGCC G^G[CT][GA]CC
928 GGTGCC
BanII 39 GAGCCC G[GA]GC[CT]^C
637 GAGCCC
BbsI 199 GAAGAC GAAGAC(2/6)
25 GTCTTC
BpuAI 199 GAAGAC GAAGAC(2/6)
25 GTCTTC
BsaWI 584 ACCGGT [AT]^CCGG[AT]
1086 TCCGGA
BsgI 592 CTGCAC GTGCAG(16/14)
514 GTGCAG
BsrFI 308 ACCGGC [GA]^CCGG[CT]
584 ACCGGT
BsrI 744 CCAGT ACTGG(1/-1)
1074 CCAGT
Cfr10I 308 ACCGGC [GA]^CCGG[CT]
584 ACCGGT
DdeI 316 CTGAG C^T[ACGT]AG
835 CTCAG
EarI 730 CTCTTC CTCTTC(1/4)
466 GAAGAG
Ksp632I 730 CTCTTC CTCTTC(1/4)
466 GAAGAG
PaeR7I 598 CTCGAG C^TCGAG
992 CTCGAG
PstI 301 CTGCAG CTGCA^G
388 CTGCAG
StuI 257 AGGCCT AGG^CCT
416 AGGCCT
TspRI 386 CACTG CA[GC]TG[ACGT][ACGT]^
830 CACTG
XhoI 598 CTCGAG C^TCGAG
992 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PshAI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI TaqI
TfiI TseI Tsp45I TthHB8I