Restriction map for
pFN21AB6630
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiEI BsiWI BsmBI BspDI BspHI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 63 CGCG CG^CG
AcyI 586 GACGCC G[GA]^CG[CT]C
AseI 922 ATTAAT AT^TAAT
AsnI 922 ATTAAT AT^TAAT
Asp700I 296 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 592 GTGCTC G[AT]GC[AT]^C
AvaI 267 CCCGAG C^[CT]CG[GA]G
BanII 77 GAGCCC G[GA]GC[CT]^C
BbsI 1069 GTCTTC GAAGAC(2/6)
BcnI 241 CCGGG CC^[GC]GG
BfaI 1120 CTAG C^TAG
BglI 628 GCCCACAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1069 GTCTTC GAAGAC(2/6)
BsaHI 586 GACGCC G[GA]^CG[CT]C
BsaI 164 GAGACC GGTCTC(1/5)
BsiHKAI 592 GTGCTC G[AT]GC[AT]^C
BsmAI 164 GAGAC GTCTC(1/5)
BsoBI 267 CCCGAG C^[CT]CG[GA]G
Bsp1407I 434 TGTACA T^GTACA
BsrDI 1212 GCAATG GCAATG(2/0)
BsrFI 42 GCCGGT [GA]^CCGG[CT]
BsrGI 434 TGTACA T^GTACA
BstAPI 180 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 63 CGCG CG^CG
BstXI 1274 CCAGCCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1100 AGATCC [GA]^GATC[CT]
Cfr10I 42 GCCGGT [GA]^CCGG[CT]
DraIII 1240 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 344 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 637 GCGGG CCCGC(4/6)
HaeII 1013 AGCGCC [GA]GCGC^[CT]
Hin1I 586 GACGCC G[GA]^CG[CT]C
MaeI 1120 CTAG C^TAG
MaeII 98 ACGT A^CGT
MflI 1100 AGATCC [GA]^GATC[CT]
MvnI 63 CGCG CG^CG
NciI 241 CCGGG CC^[GC]GG
NspI 533 ACATGC [GA]CATG^[CT]
PleI 385 GACTC GAGTC(4/5)
PshBI 922 ATTAAT AT^TAAT
ScaI 668 AGTACT AGT^ACT
SspBI 434 TGTACA T^GTACA
TaqI 70 TCGA T^CGA
Tsp509I 925 AATT ^AATT
TthHB8I 70 TCGA T^CGA
XhoII 1100 AGATCC [GA]^GATC[CT]
XmnI 296 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 278 TCCGGA T^CCGGA
500 TCCGGA
AlwNI 130 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1138 CAGGGACTG
BalI 680 TGGCCA TGG^CCA
1019 TGGCCA
BanI 1217 GGCACC G^G[CT][GA]CC
1297 GGCACC
BsaWI 278 TCCGGA [AT]^CCGG[AT]
500 TCCGGA
BseAI 278 TCCGGA T^CCGGA
500 TCCGGA
BsmI 262 GAATGC GAATGC(1/-1)
778 GCATTC
BspEI 278 TCCGGA T^CCGGA
500 TCCGGA
BspMI 413 ACCTGC ACCTGC(4/8)
1300 ACCTGC
DsaI 440 CCGTGG C^C[GA][CT]GG
1244 CCGTGG
HphI 287 TCACC GGTGA(8/7)
614 TCACC
MaeIII 95 GTGAC ^GT[ACGT]AC
613 GTCAC
MluNI 680 TGGCCA TGG^CCA
1019 TGGCCA
MroI 278 TCCGGA T^CCGGA
500 TCCGGA
MscI 680 TGGCCA TGG^CCA
1019 TGGCCA
MseI 374 TTAA T^TAA
923 TTAA
PflMI 878 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1137 CCAGGGACTGG
PvuII 1105 CAGCTG CAG^CTG
1186 CAGCTG
SapI 118 GAAGAGC GCTCTTC(1/4)
594 GCTCTTC
SbfI 135 CCTGCAGG CCTGCA^GG
1301 CCTGCAGG
Sse8387I 135 CCTGCAGG CCTGCA^GG
1301 CCTGCAGG
StuI 352 AGGCCT AGG^CCT
708 AGGCCT
TfiI 272 GATTC G^A[AT]TC
953 GAATC
Tru9I 374 TTAA T^TAA
923 TTAA
Tsp45I 95 GTGAC ^GT[GC]AC
613 GTCAC
Van91I 878 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1137 CCAGGGACTGG
XcmI 350 CCAGGCCTAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
827 CCAGACACTTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI TspRI