(Circular) (Six-base) MAPSORT of: pFN21AB6630.seq Check: 6773 from: 1 to: 6025 pFN21AB6630 6025 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3314 3314 Size: 6025 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3835 278 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3368 1937 Size: 1431 4594 Acc65I G'GTAC_C Cuts at: 3346 3346 Size: 6025 AclI AA'CG_TT Cuts at: 1563 4152 4525 1563 Size: 2589 373 3063 Fragments arranged by size: 3063 2589 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2444 3244 4088 5472 5740 1632 Size: 812 800 844 1384 268 1917 Fragments arranged by size: 1917 1384 844 812 800 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5828 AflIII A'CryG_T Cuts at: 1203 4924 5901 1203 Size: 3721 977 1327 Fragments arranged by size: 3721 1327 977 AgeI A'CCGG_T Cuts at: 4856 4856 Size: 6025 AhdI GACnn_n'nnGTC Cuts at: 4754 4754 Size: 6025 AleI CACnn'nnGTG Cuts at: 1166 3228 1166 Size: 2062 3963 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6025 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2378 2410 2378 Size: 32 5993 AlwNI CAG_nnn'CTG Cuts at: 2140 3148 3768 5340 2140 Size: 1008 620 1572 2825 Fragments arranged by size: 2825 1572 1008 620 ApaLI G'TGCA_C Cuts at: 4082 5238 4082 Size: 1156 4869 ApoI r'AATT_y Cuts at: 1493 3334 3560 3712 5821 1493 Size: 1841 226 152 2109 1697 Fragments arranged by size: 2109 1841 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2928 4579 160 Size: 872 1896 1651 1606 Fragments arranged by size: 1896 1651 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 AvaI C'yCGr_G Cuts at: 1870 1943 2272 3350 1870 Size: 73 329 1078 4545 Fragments arranged by size: 4545 1078 329 73 BamHI G'GATC_C Cuts at: 3355 3722 3355 Size: 367 5658 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3222 3302 3346 4801 618 Size: 325 217 627 1435 80 44 1455 1842 Fragments arranged by size: 1842 1455 1435 627 325 217 80 44 BanII G_rGCy'C Cuts at: 1391 2086 3344 1391 Size: 695 1258 4072 Fragments arranged by size: 4072 1258 695 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3067 928 Size: 971 1168 3886 Fragments arranged by size: 3886 1168 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4216 4250 1903 Size: 34 50 34 2195 34 3678 Fragments arranged by size: 3678 2195 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3917 5127 3917 Size: 1210 4815 BclI T'GATC_A Cuts at: 1548 1548 Size: 6025 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2639 4636 136 Size: 107 122 71 631 605 967 1997 1525 Fragments arranged by size: 1997 1525 967 631 605 122 107 71 BglII A'GATC_T Cuts at: 6020 6020 Size: 6025 BlpI GC'TnA_GC Cuts at: 3442 3442 Size: 6025 Bme1580I G_kGCm'C Cuts at: 1790 2180 2572 3305 4086 5242 1790 Size: 390 392 733 781 1156 2573 Fragments arranged by size: 2573 1156 781 733 392 390 BmgBI CAC'GTC Cuts at: 1318 2104 1318 Size: 786 5239 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3756 4714 442 Size: 416 53 83 399 24 2339 958 1753 Fragments arranged by size: 2339 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6025 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2100 2137 2753 2784 3215 4685 1239 Size: 861 37 616 31 431 1470 2579 Fragments arranged by size: 2579 1470 861 616 431 37 31 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4150 5015 5313 4150 Size: 865 298 4862 Fragments arranged by size: 4862 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2163 4688 882 Size: 617 664 2525 2219 Fragments arranged by size: 2525 2219 664 617 BsaAI yAC'GTr Cuts at: 493 1204 3816 493 Size: 711 2612 2702 Fragments arranged by size: 2702 2612 711 BsaBI GATnn'nnATC Cuts at: 6019 6019 Size: 6025 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2592 3832 4214 4849 Size: 53 83 186 936 1059 1240 382 635 Cuts at: 4849 275 Size: 1451 Fragments arranged by size: 1451 1240 1059 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2283 2505 4458 4856 5130 5277 5688 2283 Size: 222 1953 398 274 147 411 2620 Fragments arranged by size: 2620 1953 411 398 274 222 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2444 2474 1178 Size: 30 165 30 1041 30 4729 Fragments arranged by size: 4729 1041 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2449 2596 2723 1186 Size: 1263 147 127 4488 Fragments arranged by size: 4488 1263 147 127 BseYI C'CCAG_C Cuts at: 2859 5228 5724 2859 Size: 2369 496 3160 Fragments arranged by size: 3160 2369 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2308 2503 3209 3227 1163 Size: 573 572 195 706 18 3961 Fragments arranged by size: 3961 706 573 572 195 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3475 4236 5264 1540 Size: 275 186 1474 761 1028 2301 Fragments arranged by size: 2301 1474 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2601 4086 4171 5242 2601 Size: 1485 85 1071 3384 Fragments arranged by size: 3384 1485 1071 85 BsmI GAATG_Cn' Cuts at: 2273 2783 3536 3629 2273 Size: 510 753 93 4669 Fragments arranged by size: 4669 753 510 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2180 2572 2601 3305 3344 4086 Size: 399 296 94 392 29 704 39 742 Cuts at: 4086 4171 5242 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 704 399 392 296 94 85 39 29 BspEI T'CCGG_A Cuts at: 2283 2505 5688 2283 Size: 222 3183 2620 Fragments arranged by size: 3183 2620 222 BspHI T'CATG_A Cuts at: 3914 5644 3914 Size: 1730 4295 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2427 3314 3380 844 Size: 1583 887 66 3489 Fragments arranged by size: 3489 1583 887 66 BsrBI CCG'CTC Cuts at: 1394 1823 3912 1394 Size: 429 2089 3507 Fragments arranged by size: 3507 2089 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3224 4520 4694 65 Size: 1183 1976 1296 174 1396 Fragments arranged by size: 1976 1396 1296 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2047 4669 4856 1700 Size: 110 237 2622 187 2869 Fragments arranged by size: 2869 2622 237 187 110 BsrGI T'GTAC_A Cuts at: 96 2439 96 Size: 2343 3682 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6025 BssSI C'ACGA_G Cuts at: 4085 5097 4085 Size: 1012 5013 BstAPI GCAn_nnn'nTGC Cuts at: 794 2191 794 Size: 1397 4628 BstBI TT'CG_AA Cuts at: 4829 4829 Size: 6025 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6025 BstXI CCAn_nnnn'nTGG Cuts at: 3286 3286 Size: 6025 BstYI r'GATC_y Cuts at: 1972 3105 3355 3722 4107 4124 5576 5662 5674 Size: 1133 250 367 385 17 1452 86 12 Cuts at: 5674 6020 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1133 385 367 346 250 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2445 3249 513 Size: 552 1380 804 3289 Fragments arranged by size: 3289 1380 804 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2643 487 Size: 683 56 1417 3869 Fragments arranged by size: 3869 1417 683 56 BtsI GCAGTG_nn' Cuts at: 2515 3239 3541 4335 4355 2515 Size: 724 302 794 20 4185 Fragments arranged by size: 4185 794 724 302 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6025 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2099 2134 529 Size: 35 1535 35 4420 Fragments arranged by size: 4420 1535 35 35 DraI TTT'AAA Cuts at: 3329 3329 Size: 6025 DraIII CAC_nnn'GTG Cuts at: 3250 3250 Size: 6025 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5032 809 Size: 507 3716 1802 Fragments arranged by size: 3716 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2685 3024 3156 Size: 54 1425 50 275 18 855 339 132 Cuts at: 3156 3472 4361 5990 8 Size: 316 889 1629 43 Fragments arranged by size: 1629 1425 889 855 339 316 275 132 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3472 1537 Size: 275 1660 4090 Fragments arranged by size: 4090 1660 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2118 2242 2398 2606 3955 1353 Size: 765 124 156 208 1349 3423 Fragments arranged by size: 3423 1349 765 208 156 124 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4612 4986 5132 387 Size: 1064 138 3023 374 146 1280 Fragments arranged by size: 3023 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2137 2444 2753 2784 3215 3244 Size: 393 468 37 307 309 31 431 29 Cuts at: 3244 4088 4685 5472 5740 1239 Size: 844 597 787 268 1524 Fragments arranged by size: 1524 844 787 597 468 431 393 309 307 268 37 31 29 EcoNI CCTnn'n_nnAGG Cuts at: 2353 2353 Size: 6025 EcoRI G'AATT_C Cuts at: 3334 3712 3334 Size: 378 5647 FspI TGC'GCA Cuts at: 4531 4531 Size: 6025 HaeII r_GCGC'y Cuts at: 1678 3022 5172 1678 Size: 1344 2150 2531 Fragments arranged by size: 2531 2150 1344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2324 2356 4666 4698 4740 Size: 32 135 32 752 32 2310 32 42 Cuts at: 4740 4772 1373 Size: 32 2626 Fragments arranged by size: 2626 2310 752 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3369 3615 669 Size: 988 281 1431 246 3079 Fragments arranged by size: 3079 1431 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6025 HpaI GTT'AAC Cuts at: 3615 3615 Size: 6025 KpnI G_GTAC'C Cuts at: 3350 3350 Size: 6025 MfeI C'AATT_G Cuts at: 3624 3624 Size: 6025 MluI A'CGCG_T Cuts at: 5901 5901 Size: 6025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 2601 5139 5323 1305 Size: 1116 180 2538 184 2007 Fragments arranged by size: 2538 2007 1116 184 180 MscI TGG'CCA Cuts at: 10 64 1489 1832 2687 3026 10 Size: 54 1425 343 855 339 3009 Fragments arranged by size: 3009 1425 855 343 339 54 MslI CAynn'nnrTG Cuts at: 518 1166 2537 2788 3228 3983 4342 4501 518 Size: 648 1371 251 440 755 359 159 2042 Fragments arranged by size: 2042 1371 755 648 440 359 251 159 MspA1I CmG'CkG Cuts at: 1606 2029 3112 3193 3441 4118 5266 5511 1606 Size: 423 1083 81 248 677 1148 245 2120 Fragments arranged by size: 2120 1148 1083 677 423 248 245 81 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5473 NdeI CA'TA_TG Cuts at: 387 387 Size: 6025 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6025 NotI GC'GGCC_GC Cuts at: 3472 3472 Size: 6025 NspI r_CATG'y Cuts at: 1132 1229 2542 3383 4928 1132 Size: 97 1313 841 1545 2229 Fragments arranged by size: 2229 1545 1313 841 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6025 PciI A'CATG_T Cuts at: 4924 4924 Size: 6025 PflMI CCAn_nnn'nTGG Cuts at: 1108 2889 3148 1108 Size: 1781 259 3985 Fragments arranged by size: 3985 1781 259 PmeI GTTT'AAAC Cuts at: 3329 3329 Size: 6025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4094 4126 5607 5639 4094 Size: 32 1481 32 4480 Fragments arranged by size: 4480 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6025 PsiI TTA'TAA Cuts at: 3015 3595 3015 Size: 580 5445 PstI C_TGCA'G Cuts at: 830 2034 2145 3311 3377 830 Size: 1204 111 1166 66 3478 Fragments arranged by size: 3478 1204 1166 111 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6025 PvuII CAG'CTG Cuts at: 3112 3193 3112 Size: 81 5944 SalI G'TCGA_C Cuts at: 1936 3367 1936 Size: 1431 4594 SapI GCTCTTCn'nnn_ Cuts at: 2118 2606 2118 Size: 488 5537 SbfI CC_TGCA'GG Cuts at: 2145 3311 3377 2145 Size: 1166 66 4793 Fragments arranged by size: 4793 1166 66 ScaI AGT'ACT Cuts at: 2675 4273 5985 2675 Size: 1598 1712 2715 Fragments arranged by size: 2715 1712 1598 SfcI C'TryA_G Cuts at: 826 1045 2030 2141 2452 3307 3373 4508 5189 Size: 219 985 111 311 855 66 1135 681 Cuts at: 5189 5380 826 Size: 191 1471 Fragments arranged by size: 1471 1135 985 855 681 311 219 191 111 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6025 SmaI CCC'GGG Cuts at: 1872 3352 1872 Size: 1480 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4129 5030 5292 820 Size: 197 926 2186 901 262 1553 Fragments arranged by size: 2186 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3816 493 Size: 3323 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6025 SphI G_CATG'C Cuts at: 1132 3383 1132 Size: 2251 3774 SspI AAT'ATT Cuts at: 5 52 3949 5 Size: 47 3897 2081 Fragments arranged by size: 3897 2081 47 StuI AGG'CCT Cuts at: 1517 2359 2715 1517 Size: 842 356 4827 Fragments arranged by size: 4827 842 356 StyI C'CwwG_G Cuts at: 513 1065 2485 3130 3273 3464 513 Size: 552 1420 645 143 191 3074 Fragments arranged by size: 3074 1420 645 552 191 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2844 4052 4069 4222 4407 4852 325 Size: 1255 1264 1208 17 153 185 445 1498 Fragments arranged by size: 1498 1264 1255 1208 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2439 2673 4271 Size: 274 80 33 51 1443 462 234 1598 Cuts at: 4271 5983 96 Size: 1712 138 Fragments arranged by size: 1712 1598 1443 462 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2884 2916 3138 3170 5985 6017 2884 Size: 32 222 32 2815 32 2892 Fragments arranged by size: 2892 2815 222 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6025 XbaI T'CTAG_A Cuts at: 3361 3361 Size: 6025 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2362 2839 2362 Size: 477 5548 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6025 XmaI C'CCGG_G Cuts at: 1870 3350 1870 Size: 1480 4545 XmnI GAAnn'nnTTC Cuts at: 2305 4154 2305 Size: 1849 4176 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3833 276 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI