Restriction map for
pFN21AB6625
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
Acc65I AccI AccIII AcsI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BbeI BbrPI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eam1105I EarI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
MaeI MfeI MluI MroI MslI
MunI NarI NciI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PstI PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 865 GACGTC GACGT^C
AclI 58 AACGTT AA^CGTT
Alw44I 436 GTGCAC G^TGCAC
ApaLI 436 GTGCAC G^TGCAC
AviII 762 TGCGCA TGC^GCA
BamHI 697 GGATCC G^GATCC
BanI 1156 GGCACC G^G[CT][GA]CC
BbsI 493 GAAGAC GAAGAC(2/6)
BlpI 1003 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1003 GCTGAGC GC^T[ACGT]AGC
BpuAI 493 GAAGAC GAAGAC(2/6)
BsaAI 547 TACGTG [CT]AC^GT[GA]
BsaBI 274 GATTACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 43 GTGCAG GTGCAG(16/14)
BsiEI 1178 CGGCCG CG[GA][CT]^CG
BsmI 237 GAATGC GAATGC(1/-1)
BspMI 387 ACCTGC ACCTGC(4/8)
CelII 1003 GCTGAGC GC^T[ACGT]AGC
EagI 1178 CGGCCG C^GGCCG
EclXI 1178 CGGCCG C^GGCCG
Eco52I 1178 CGGCCG C^GGCCG
FspI 762 TGCGCA TGC^GCA
HaeII 682 GGCGCT [GA]GCGC^[CT]
MamI 274 GATTACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 29 TTAA T^TAA
NaeI 1176 GCCGGC GCC^GGC
NcoI 431 CCATGG C^CATGG
NgoMIV 1176 GCCGGC G^CCGGC
Psp1406I 58 AACGTT AA^CGTT
SacI 1077 GAGCTC GAGCT^C
SfaNI 113 GCATC GCATC(5/9)
TfiI 540 GAATC G^A[AT]TC
Tru9I 29 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 306 GTAC GT^AC
621 GTAC
ApaI 530 GGGCCC GGGCC^C
897 GGGCCC
BalI 2 TGGCCA TGG^CCA
450 TGGCCA
BbvCI 165 CCTCAGC CCTCAGC(-5/-2)
409 CCTCAGC
BsrFI 656 GCCGGT [GA]^CCGG[CT]
1176 GCCGGC
BstF5I 48 GGATG GGATG(2/0)
856 GGATG
BstYI 119 GGATCT [GA]^GATC[CT]
697 GGATCC
Cfr10I 656 GCCGGT [GA]^CCGG[CT]
1176 GCCGGC
DrdI 420 GACGCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1017 GACCCGCAAGTC
FokI 48 GGATG GGATG(9/13)
856 GGATG
HapII 657 CCGG C^CGG
1177 CCGG
HpaII 657 CCGG C^CGG
1177 CCGG
KspI 147 CCGCGG CCGC^GG
320 CCGCGG
MflI 119 GGATCT [GA]^GATC[CT]
697 GGATCC
MluNI 2 TGGCCA TGG^CCA
450 TGGCCA
MscI 2 TGGCCA TGG^CCA
450 TGGCCA
MspI 657 CCGG C^CGG
1177 CCGG
NlaIII 432 CATG CATG^
609 CATG
PvuII 1041 CAGCTG CAG^CTG
1086 CAGCTG
RsaI 306 GTAC GT^AC
621 GTAC
SacII 147 CCGCGG CCGC^GG
320 CCGCGG
SmlI 214 CTTGAG C^T[CT][GA]AG
754 CTCAAG
XhoII 119 GGATCT [GA]^GATC[CT]
697 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeIII HgaI HhaI Hin1I HinP1I
HinfI HphI ItaI MaeII MaeIII
MboI MboII MnlI MspA1I MvaI
MvnI MwoI NdeII NlaIV PleI
Sau3AI Sau96I ScrFI StyI TseI
Tsp45I TspRI