(Circular) (Six-base) MAPSORT of: pFN21AB6625.seq Check: 1314 from: 1 to: 5923 pFN21AB6625 5923 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2401 2401 Size: 5923 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2874 3733 278 Size: 53 83 186 936 1338 859 2468 Fragments arranged by size: 2468 1338 936 859 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 2064 4050 4423 1563 Size: 501 1986 373 3063 Fragments arranged by size: 3063 1986 501 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3986 5370 5638 1632 Size: 2354 1384 268 1917 Fragments arranged by size: 2354 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AlwNI CAG_nnn'CTG Cuts at: 3666 5238 3666 Size: 1572 4351 ApaI G_GGCC'C Cuts at: 2539 2906 2539 Size: 367 5556 ApaLI G'TGCA_C Cuts at: 2441 3980 5136 2441 Size: 1539 1156 3228 Fragments arranged by size: 3228 1539 1156 ApoI r'AATT_y Cuts at: 1493 3232 3458 3610 5719 1493 Size: 1739 226 152 2109 1697 Fragments arranged by size: 2109 1739 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2140 2629 3034 3248 1870 Size: 73 197 489 405 214 4545 Fragments arranged by size: 4545 489 405 214 197 73 BamHI G'GATC_C Cuts at: 2702 3253 3620 2702 Size: 551 367 5005 Fragments arranged by size: 5005 551 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3161 3244 4699 618 Size: 325 217 627 1374 83 1455 1842 Fragments arranged by size: 1842 1455 1374 627 325 217 83 BanII G_rGCy'C Cuts at: 1391 2539 2906 3015 3086 3110 3242 1391 Size: 1148 367 109 71 24 132 4072 Fragments arranged by size: 4072 1148 367 132 109 71 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2505 928 Size: 971 606 4346 Fragments arranged by size: 4346 971 606 BbvCI CC'TCA_GC Cuts at: 2171 2415 2171 Size: 244 5679 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4114 4148 1903 Size: 34 2177 34 3678 Fragments arranged by size: 3678 2177 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4534 136 Size: 107 122 71 631 605 2862 1525 Fragments arranged by size: 2862 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 3009 3340 3009 Size: 331 5592 Bme1580I G_kGCm'C Cuts at: 1790 2445 2539 2906 3164 3192 3984 5140 1790 Size: 655 94 367 258 28 792 1156 2573 Fragments arranged by size: 2573 1156 792 655 367 258 94 28 BmgBI CAC'GTC Cuts at: 1318 3054 1318 Size: 1736 4187 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3229 3654 4612 Size: 416 53 83 399 24 1812 425 958 Cuts at: 4612 442 Size: 1753 Fragments arranged by size: 1812 1753 958 425 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2285 2402 2656 2673 2794 4583 1239 Size: 914 132 117 254 17 121 1789 2579 Fragments arranged by size: 2579 1789 914 254 132 121 117 17 Bpu10I CC'TnA_GC Cuts at: 2171 2415 2171 Size: 244 5679 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2240 2744 4048 4913 5211 2240 Size: 504 1304 865 298 2952 Fragments arranged by size: 2952 1304 865 504 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2382 2824 2987 4586 882 Size: 617 883 442 163 1599 2219 Fragments arranged by size: 2219 1599 883 617 442 163 BsaAI yAC'GTr Cuts at: 493 1204 2554 3714 493 Size: 711 1350 1160 2702 Fragments arranged by size: 2702 1350 1160 711 BsaBI GATnn'nnATC Cuts at: 2283 5917 2283 Size: 3634 2289 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2723 2831 2871 3730 Size: 53 83 186 936 1190 108 40 859 Cuts at: 3730 4112 4747 275 Size: 382 635 1451 Fragments arranged by size: 1451 1190 936 859 635 382 186 108 83 53 40 BsaWI w'CCGG_w Cuts at: 4356 4754 5028 5175 5586 4356 Size: 398 274 147 411 4693 Fragments arranged by size: 4693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2507 2537 1178 Size: 30 165 30 1104 30 4564 Fragments arranged by size: 4564 1104 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2470 3091 3094 1186 Size: 1284 621 3 4015 Fragments arranged by size: 4015 1284 621 3 BseYI C'CCAG_C Cuts at: 2731 3093 3204 5126 5622 2731 Size: 362 111 1922 496 3032 Fragments arranged by size: 3032 1922 496 362 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 1163 Size: 573 333 5017 Fragments arranged by size: 5017 573 333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3186 3373 4134 5162 1540 Size: 275 186 1185 187 761 1028 2301 Fragments arranged by size: 2301 1185 1028 761 275 187 186 BsiHKAI G_wGCw'C Cuts at: 2141 2445 3086 3984 4069 5140 2141 Size: 304 641 898 85 1071 2924 Fragments arranged by size: 2924 1071 898 641 304 85 BsmI GAATG_Cn' Cuts at: 2248 3434 3527 2248 Size: 1186 93 4644 Fragments arranged by size: 4644 1186 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2445 2539 2906 3015 3086 3110 Size: 399 351 304 94 367 109 71 24 Cuts at: 3110 3164 3192 3242 3984 4069 5140 1391 Size: 54 28 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 367 351 304 109 94 85 71 54 50 28 24 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2401 3278 844 Size: 1557 877 3489 Fragments arranged by size: 3489 1557 877 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2661 3181 4567 4754 1700 Size: 110 851 520 1386 187 2869 Fragments arranged by size: 2869 1386 851 520 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2124 2702 3253 3620 4005 4022 5474 5560 Size: 152 578 551 367 385 17 1452 86 Cuts at: 5560 5572 5918 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 578 551 385 367 346 152 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2152 2325 2436 513 Size: 552 939 148 173 111 4000 Fragments arranged by size: 4000 939 552 173 148 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 3180 3439 4233 4253 3180 Size: 259 794 20 4850 Fragments arranged by size: 4850 794 259 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2431 3028 4930 809 Size: 507 1115 597 1902 1802 Fragments arranged by size: 1902 1802 1115 597 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2358 2455 Size: 54 1425 50 275 18 177 351 97 Cuts at: 2455 3183 3370 4259 5888 8 Size: 728 187 889 1629 43 Fragments arranged by size: 1629 1425 889 728 351 275 187 177 97 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3183 3370 1537 Size: 275 1371 187 4090 Fragments arranged by size: 4090 1371 275 187 EarI CTCTTCn'nnn_ Cuts at: 1353 3853 1353 Size: 2500 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3084 3084 Size: 5923 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2285 2402 2656 2673 2794 3986 Size: 393 521 132 117 254 17 121 1192 Cuts at: 3986 4583 5370 5638 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1192 787 597 521 393 268 254 132 121 117 17 EcoO109I rG'GnC_Cy Cuts at: 2535 2902 2903 2535 Size: 367 1 5555 Fragments arranged by size: 5555 367 1 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 2769 4429 2769 Size: 1660 4263 HaeII r_GCGC'y Cuts at: 1678 2691 5070 1678 Size: 1013 2379 2531 Fragments arranged by size: 2531 2379 1013 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2398 2430 2507 2539 2792 Size: 32 135 32 826 32 77 32 253 Cuts at: 2792 2824 2903 2935 4564 4596 4638 4670 1373 Size: 32 79 32 1629 32 42 32 2626 Fragments arranged by size: 2626 1629 826 253 135 79 77 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2457 10 Size: 54 1425 343 177 448 3476 Fragments arranged by size: 3476 1425 448 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3881 4240 4399 518 Size: 648 2715 359 159 2042 Fragments arranged by size: 2715 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2154 2327 2946 3048 3093 3339 4016 5164 Size: 548 173 619 102 45 246 677 1148 Cuts at: 5164 5409 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 619 548 246 245 173 102 45 NaeI GCC'GGC Cuts at: 3183 3183 Size: 5923 NcoI C'CATG_G Cuts at: 513 1065 2004 2436 513 Size: 552 939 432 4000 Fragments arranged by size: 4000 939 552 432 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NgoMIV G'CCGG_C Cuts at: 3181 3181 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 3281 4826 1132 Size: 97 2052 1545 2229 Fragments arranged by size: 2229 2052 1545 97 PasI CC'CwG_GG Cuts at: 1348 2539 1348 Size: 1191 4732 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5923 PfoI T'CCnGG_A Cuts at: 2262 2262 Size: 5923 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PspOMI G'GGCC_C Cuts at: 2535 2902 2535 Size: 367 5556 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 3048 3093 3048 Size: 45 5878 SacI G_AGCT'C Cuts at: 3086 3086 Size: 5923 SacII CC_GC'GG Cuts at: 2155 2328 2155 Size: 173 5750 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 3271 4406 5087 5278 826 Size: 219 2226 1135 681 191 1471 Fragments arranged by size: 2226 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2219 2759 4027 4928 5190 820 Size: 197 926 276 540 1268 901 262 1553 Fragments arranged by size: 1553 1268 926 901 540 276 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2004 2436 2679 2835 2907 3362 513 Size: 552 939 432 243 156 72 455 3074 Fragments arranged by size: 3074 939 552 455 432 243 156 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3950 3967 4120 4305 4750 325 Size: 1255 2370 17 153 185 445 1498 Fragments arranged by size: 2370 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2310 2625 4169 Size: 274 80 33 51 1443 333 315 1544 Cuts at: 4169 5881 96 Size: 1712 138 Fragments arranged by size: 1712 1544 1443 333 315 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5883 5915 5883 Size: 32 5891 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2872 3731 276 Size: 53 83 186 936 1338 859 2468 Fragments arranged by size: 2468 1338 936 859 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI