################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AlwI AocI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccII 121 CGCG CG^CG AcsI 232 AAATTT [GA]^AATT[CT] AcyI 951 GGCGCC G[GA]^CG[CT]C Alw44I 763 GTGCAC G^TGCAC ApaI 329 GGGCCC GGGCC^C ApaLI 763 GTGCAC G^TGCAC ApoI 232 AAATTT [GA]^AATT[CT] BbeI 951 GGCGCC GGCGC^C BbsI 489 GAAGAC GAAGAC(2/6) BpuAI 489 GAAGAC GAAGAC(2/6) BsaHI 951 GGCGCC G[GA]^CG[CT]C BsiEI 74 CGACCG CG[GA][CT]^CG BsrDI 110 GCAATG GCAATG(2/0) BstEII 57 GGTCACC G^GT[ACGT]ACC BstPI 57 GGTCACC G^GT[ACGT]ACC BstUI 121 CGCG CG^CG BstXI 1268 CCAACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 308 CCATGG C^C[GA][CT]GG EarI 985 GAAGAG CTCTTC(1/4) EcoO65I 57 GGTCACC G^GT[ACGT]ACC Hin1I 951 GGCGCC G[GA]^CG[CT]C HinfI 754 GAGTC G^A[ACGT]TC KasI 951 GGCGCC G^GCGCC Ksp632I 985 GAAGAG CTCTTC(1/4) MseI 512 TTAA T^TAA MvnI 121 CGCG CG^CG NarI 951 GGCGCC GG^CGCC NcoI 308 CCATGG C^CATGG NspI 1256 GCATGT [GA]CATG^[CT] PflMI 447 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 754 GAGTC GAGTC(4/5) SfoI 951 GGCGCC GGC^GCC SmlI 915 CTCAAG C^T[CT][GA]AG StuI 41 AGGCCT AGG^CCT Tru9I 512 TTAA T^TAA Tsp509I 233 AATT ^AATT Van91I 447 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1098 CCAGGGGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AlwNI 531 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 902 CAGCTGCTG Aor51HI 30 AGCGCT AGC^GCT 665 AGCGCT AvaI 768 CCCGAG C^[CT]CG[GA]G 1180 CCCGAG AvaII 210 GGACC G^G[AT]CC 457 GGACC BcnI 627 CCGGG CC^[GC]GG 657 CCGGG BglI 592 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1247 GCCATTTTGGC BglII 930 AGATCT A^GATCT 1166 AGATCT BsaAI 46 TACGTG [CT]AC^GT[GA] 1273 TACGTG BsaI 880 GAGACC GGTCTC(1/5) 1021 GAGACC BsmAI 880 GAGAC GTCTC(1/5) 1021 GAGAC BsmFI 456 GGGAC GGGAC(10/14) 69 GTCCC BsoBI 768 CCCGAG C^[CT]CG[GA]G 1180 CCCGAG BsrFI 948 GCCGGC [GA]^CCGG[CT] 1063 GCCGGC BstYI 930 AGATCT [GA]^GATC[CT] 1166 AGATCT Cfr10I 948 GCCGGC [GA]^CCGG[CT] 1063 GCCGGC Eco47III 30 AGCGCT AGC^GCT 665 AGCGCT EcoT14I 308 CCATGG C^C[AT][AT]GG 398 CCAAGG MaeIII 58 GTCAC ^GT[ACGT]AC 337 GTGAC MboII 489 GAAGA GAAGA(8/7) 985 GAAGA MflI 930 AGATCT [GA]^GATC[CT] 1166 AGATCT MslI 117 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1200 CATCGAGGTG NaeI 948 GCCGGC GCC^GGC 1063 GCCGGC NciI 627 CCGGG CC^[GC]GG 657 CCGGG NgoMIV 948 GCCGGC G^CCGGC 1063 GCCGGC PpuMI 209 AGGACCC [GA]G^G[AT]CC[CT] 456 GGGACCC PstI 198 CTGCAG CTGCA^G 856 CTGCAG PvuII 610 CAGCTG CAG^CTG 902 CAGCTG SacI 166 GAGCTC GAGCT^C 1279 GAGCTC SfaNI 380 GCATC GCATC(5/9) 1199 GCATC StyI 308 CCATGG C^C[AT][AT]GG 398 CCAAGG TaqI 1202 TCGA T^CGA 1211 TCGA Tsp45I 58 GTCAC ^GT[GC]AC 337 GTGAC TthHB8I 1202 TCGA T^CGA 1211 TCGA XhoII 930 AGATCT [GA]^GATC[CT] 1166 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AfaI AluI AspHI BalI BanI BanII BbvCI BbvI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MboI MluNI MnlI MscI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI TseI TspRI