(Circular) (Six-base) MAPSORT of: pFN21AB6610.seq Check: 5146 from: 1 to: 5995 pFN21AB6610 5995 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 1937 Size: 1401 4594 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 4122 4495 1563 Size: 2559 373 3063 Fragments arranged by size: 3063 2559 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2348 2394 4058 5442 5710 1632 Size: 716 46 1664 1384 268 1917 Fragments arranged by size: 1917 1664 1384 716 268 46 AfeI AGC'GCT Cuts at: 2037 2672 2037 Size: 635 5360 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 4894 5871 1203 Size: 3691 977 1327 Fragments arranged by size: 3691 1327 977 AgeI A'CCGG_T Cuts at: 4826 4826 Size: 5995 AhdI GACnn_n'nnGTC Cuts at: 4724 4724 Size: 5995 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2778 3081 1184 Size: 1594 303 4098 Fragments arranged by size: 4098 1594 303 AlwNI CAG_nnn'CTG Cuts at: 2541 2912 3738 5310 2541 Size: 371 826 1572 3226 Fragments arranged by size: 3226 1572 826 371 ApaI G_GGCC'C Cuts at: 2338 2338 Size: 5995 ApaLI G'TGCA_C Cuts at: 2768 4052 5208 2768 Size: 1284 1156 3555 Fragments arranged by size: 3555 1284 1156 ApoI r'AATT_y Cuts at: 1493 2237 3304 3530 3682 5791 1493 Size: 744 1067 226 152 2109 1697 Fragments arranged by size: 2109 1697 1067 744 226 152 AseI AT'TA_AT Cuts at: 160 1032 4549 160 Size: 872 3517 1606 Fragments arranged by size: 3517 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 2773 3185 3320 1870 Size: 73 830 412 135 4545 Fragments arranged by size: 4545 830 412 135 73 BamHI G'GATC_C Cuts at: 3325 3692 3325 Size: 367 5628 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2414 2956 3065 3316 4771 Size: 325 217 627 627 542 109 251 1455 Cuts at: 4771 618 Size: 1842 Fragments arranged by size: 1842 1455 627 627 542 325 251 217 109 BanII G_rGCy'C Cuts at: 1391 2092 2175 2338 2483 2855 3111 3126 3288 Size: 701 83 163 145 372 256 15 162 Cuts at: 3288 3314 1391 Size: 26 4072 Fragments arranged by size: 4072 701 372 256 163 162 145 83 26 15 BbeI G_GCGC'C Cuts at: 2960 2960 Size: 5995 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2501 928 Size: 971 602 4422 Fragments arranged by size: 4422 971 602 BbvCI CC'TCA_GC Cuts at: 2832 2846 2946 2832 Size: 14 100 5881 Fragments arranged by size: 5881 100 14 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4186 4220 1903 Size: 34 2249 34 3678 Fragments arranged by size: 3678 2249 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3887 5097 3887 Size: 1210 4785 BclI T'GATC_A Cuts at: 1548 1548 Size: 5995 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2603 3258 Size: 107 122 71 631 605 337 594 655 Cuts at: 3258 4606 136 Size: 1348 1525 Fragments arranged by size: 1525 1348 655 631 605 594 337 122 107 71 BglII A'GATC_T Cuts at: 2935 3171 5990 2935 Size: 236 2819 2940 Fragments arranged by size: 2940 2819 236 BlpI GC'TnA_GC Cuts at: 3412 3412 Size: 5995 Bme1580I G_kGCm'C Cuts at: 1790 2338 2772 3133 3139 4056 5212 1790 Size: 548 434 361 6 917 1156 2573 Fragments arranged by size: 2573 1156 917 548 434 361 6 BmgBI CAC'GTC Cuts at: 1318 2305 2458 2712 1318 Size: 987 153 254 4601 Fragments arranged by size: 4601 987 254 153 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2095 2468 3726 Size: 416 53 83 399 24 678 373 1258 Cuts at: 3726 4684 442 Size: 958 1753 Fragments arranged by size: 1753 1258 958 678 416 399 373 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2600 2639 2809 3000 4655 1239 Size: 935 426 39 170 191 1655 2579 Fragments arranged by size: 2579 1655 935 426 191 170 39 Bpu10I CC'TnA_GC Cuts at: 2018 2832 2846 2946 2018 Size: 814 14 100 5067 Fragments arranged by size: 5067 814 100 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2905 4120 4985 5283 2905 Size: 1215 865 298 3617 Fragments arranged by size: 3617 1215 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2879 3020 4658 882 Size: 617 1380 141 1638 2219 Fragments arranged by size: 2219 1638 1380 617 141 BsaAI yAC'GTr Cuts at: 493 1204 2053 3280 3786 493 Size: 711 849 1227 506 2702 Fragments arranged by size: 2702 1227 849 711 506 BsaBI GATnn'nnATC Cuts at: 5989 5989 Size: 5995 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2957 3802 4184 4819 Size: 53 83 186 936 1424 845 382 635 Cuts at: 4819 275 Size: 1451 Fragments arranged by size: 1451 1424 936 845 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4428 4826 5100 5247 5658 4428 Size: 398 274 147 411 4765 Fragments arranged by size: 4765 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2366 2396 2987 3017 1178 Size: 30 165 30 963 30 591 30 4156 Fragments arranged by size: 4156 963 591 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2171 2183 2378 2576 2579 2615 2863 2958 Size: 985 12 195 198 3 36 248 95 Cuts at: 2958 2963 3008 1186 Size: 5 45 4173 Fragments arranged by size: 4173 985 248 198 195 95 45 36 12 5 3 BseYI C'CCAG_C Cuts at: 2960 5198 5694 2960 Size: 2238 496 3261 Fragments arranged by size: 3261 2238 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2640 3002 3074 1163 Size: 573 904 362 72 4084 Fragments arranged by size: 4084 904 573 362 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2082 3445 4206 5234 1540 Size: 275 186 81 1363 761 1028 2301 Fragments arranged by size: 2301 1363 1028 761 275 186 81 BsiHKAI G_wGCw'C Cuts at: 2175 2772 3081 3288 4056 4141 5212 2175 Size: 597 309 207 768 85 1071 2958 Fragments arranged by size: 2958 1071 768 597 309 207 85 BsmI GAATG_Cn' Cuts at: 3506 3599 3506 Size: 93 5902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2092 2175 2338 2483 2772 2855 3081 Size: 399 302 83 163 145 289 83 226 Cuts at: 3081 3111 3126 3133 3139 3288 3314 4056 4141 Size: 30 15 7 6 149 26 742 85 Cuts at: 4141 5212 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 302 289 226 163 149 145 85 83 83 30 26 15 7 6 BspEI T'CCGG_A Cuts at: 5658 5658 Size: 5995 BspHI T'CATG_A Cuts at: 3884 5614 3884 Size: 1730 4265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3350 844 Size: 2506 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3882 1394 Size: 429 2059 3507 Fragments arranged by size: 3507 2059 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2122 4490 4664 65 Size: 1183 874 2368 174 1396 Fragments arranged by size: 2368 1396 1183 874 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2953 3068 4639 4826 1700 Size: 110 1143 115 1571 187 2869 Fragments arranged by size: 2869 1571 1143 187 115 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5995 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5995 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5995 BstBI TT'CG_AA Cuts at: 4799 4799 Size: 5995 BstEII G'GTnAC_C Cuts at: 1184 2062 1184 Size: 878 5117 BstXI CCAn_nnnn'nTGG Cuts at: 3280 3280 Size: 5995 BstYI r'GATC_y Cuts at: 1972 2935 3171 3325 3692 4077 4094 5546 5632 Size: 963 236 154 367 385 17 1452 86 Cuts at: 5632 5644 5990 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 963 385 367 346 236 154 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2313 513 Size: 552 939 309 4195 Fragments arranged by size: 4195 939 552 309 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5256 Fragments arranged by size: 5256 683 56 BtsI GCAGTG_nn' Cuts at: 3511 4305 4325 3511 Size: 794 20 5181 Fragments arranged by size: 5181 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2422 2457 529 Size: 35 1858 35 4067 Fragments arranged by size: 4067 1858 35 35 DraI TTT'AAA Cuts at: 3299 3299 Size: 5995 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5002 809 Size: 507 3686 1802 Fragments arranged by size: 3686 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2316 2400 2443 Size: 54 1425 50 275 18 486 84 43 Cuts at: 2443 3250 3442 4331 5960 8 Size: 807 192 889 1629 43 Fragments arranged by size: 1629 1425 889 807 486 275 192 84 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3442 1537 Size: 275 1630 4090 Fragments arranged by size: 4090 1630 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2985 3925 1353 Size: 1632 940 3423 Fragments arranged by size: 3423 1632 940 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4582 4956 5102 387 Size: 1064 138 2993 374 146 1280 Fragments arranged by size: 2993 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2173 3286 2173 Size: 1113 4882 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2348 2394 2600 2639 2809 3000 Size: 393 542 174 46 206 39 170 191 Cuts at: 3000 4058 4655 5442 5710 1239 Size: 1058 597 787 268 1524 Fragments arranged by size: 1524 1058 787 597 542 393 268 206 191 174 170 46 39 EcoO109I rG'GnC_Cy Cuts at: 2215 2334 2462 2470 2215 Size: 119 128 8 5740 Fragments arranged by size: 5740 128 119 8 EcoRI G'AATT_C Cuts at: 3304 3682 3304 Size: 378 5617 FspI TGC'GCA Cuts at: 4501 4501 Size: 5995 HaeII r_GCGC'y Cuts at: 1678 2039 2632 2674 2960 5142 1678 Size: 361 593 42 286 2182 2531 Fragments arranged by size: 2531 2182 593 361 286 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2207 2239 2744 2776 4636 Size: 32 135 32 635 32 505 32 1860 Cuts at: 4636 4668 4710 4742 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1860 635 505 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3339 3585 669 Size: 988 281 1401 246 3079 Fragments arranged by size: 3079 1401 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5995 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KasI G'GCGC_C Cuts at: 2956 2956 Size: 5995 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3594 3594 Size: 5995 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5109 5293 1305 Size: 3804 184 2007 Fragments arranged by size: 3804 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2318 2402 3252 10 Size: 54 1425 343 486 84 850 2753 Fragments arranged by size: 2753 1425 850 486 343 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 3209 3953 4312 4471 518 Size: 648 960 1083 744 359 159 2042 Fragments arranged by size: 2042 1083 960 744 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2181 2611 2617 2909 3411 4088 5236 5481 Size: 575 430 6 292 502 677 1148 245 Cuts at: 5481 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 575 502 430 292 245 6 NaeI GCC'GGC Cuts at: 2955 3070 2955 Size: 115 5880 NarI GG'CG_CC Cuts at: 2957 2957 Size: 5995 NcoI C'CATG_G Cuts at: 513 1065 2004 2313 513 Size: 552 939 309 4195 Fragments arranged by size: 4195 939 552 309 NdeI CA'TA_TG Cuts at: 387 387 Size: 5995 NgoMIV G'CCGG_C Cuts at: 2953 3068 2953 Size: 115 5880 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 3265 3353 4898 1132 Size: 97 2036 88 1545 2229 Fragments arranged by size: 2229 2036 1545 97 88 PasI CC'CwG_GG Cuts at: 1348 2928 1348 Size: 1580 4415 PciI A'CATG_T Cuts at: 4894 4894 Size: 5995 PflMI CCAn_nnn'nTGG Cuts at: 1108 2458 1108 Size: 1350 4645 PfoI T'CCnGG_A Cuts at: 2451 2451 Size: 5995 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 5577 5609 4064 Size: 32 1481 32 4450 Fragments arranged by size: 4450 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2215 2462 2215 Size: 247 5748 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5995 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PspOMI G'GGCC_C Cuts at: 2334 2334 Size: 5995 PstI C_TGCA'G Cuts at: 830 2207 2865 3347 830 Size: 1377 658 482 3478 Fragments arranged by size: 3478 1377 658 482 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 PvuII CAG'CTG Cuts at: 2617 2909 2617 Size: 292 5703 SacI G_AGCT'C Cuts at: 2175 3288 2175 Size: 1113 4882 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SanDI GG'GwC_CC Cuts at: 2462 2462 Size: 5995 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SfcI C'TryA_G Cuts at: 826 1045 2203 2422 2861 3343 4478 5159 5350 Size: 219 1158 219 439 482 1135 681 191 Cuts at: 5350 826 Size: 1471 Fragments arranged by size: 1471 1158 1135 681 482 439 219 219 191 SfoI GGC'GCC Cuts at: 2958 2958 Size: 5995 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 3322 1872 Size: 1450 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2920 4099 5000 5262 820 Size: 197 926 977 1179 901 262 1553 Fragments arranged by size: 1553 1179 977 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3786 493 Size: 3293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 3353 1132 Size: 2221 3774 SspI AAT'ATT Cuts at: 5 52 3919 5 Size: 47 3867 2081 Fragments arranged by size: 3867 2081 47 StuI AGG'CCT Cuts at: 1517 2048 1517 Size: 531 5464 StyI C'CwwG_G Cuts at: 513 1065 2004 2313 2403 3434 513 Size: 552 939 309 90 1031 3074 Fragments arranged by size: 3074 1031 939 552 309 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2096 2891 4022 4039 4192 4377 4822 Size: 1255 516 795 1131 17 153 185 445 Cuts at: 4822 325 Size: 1498 Fragments arranged by size: 1498 1255 1131 795 516 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4241 5953 96 Size: 274 80 33 51 1443 2264 1712 138 Fragments arranged by size: 2264 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2366 2398 5955 5987 2366 Size: 32 3557 32 2374 Fragments arranged by size: 3557 2374 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 3331 3331 Size: 5995 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3110 3110 Size: 5995 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 3320 1870 Size: 1450 4545 XmnI GAAnn'nnTTC Cuts at: 4124 4124 Size: 5995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI