(Circular) (Six-base) MAPSORT of: pFN21AB6606.seq Check: 344 from: 1 to: 5998 pFN21AB6606 5998 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2072 2072 Size: 5998 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3341 1937 Size: 1404 4594 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2624 4061 5445 5713 1632 Size: 992 1437 1384 268 1917 Fragments arranged by size: 1917 1437 1384 992 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 4897 5874 1203 Size: 3694 977 1327 Fragments arranged by size: 3694 1327 977 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 4727 4727 Size: 5998 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5998 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2307 1184 Size: 1123 4875 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2528 2560 2528 Size: 32 5966 AlwNI CAG_nnn'CTG Cuts at: 2164 3133 3741 5313 2164 Size: 969 608 1572 2849 Fragments arranged by size: 2849 1572 969 608 ApaI G_GGCC'C Cuts at: 2873 2873 Size: 5998 ApaLI G'TGCA_C Cuts at: 4055 5211 4055 Size: 1156 4842 ApoI r'AATT_y Cuts at: 1493 2288 3307 3533 3685 5794 1493 Size: 795 1019 226 152 2109 1697 Fragments arranged by size: 2109 1697 1019 795 226 152 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 2034 2118 2652 3195 3242 3323 1870 Size: 73 91 84 534 543 47 81 4545 Fragments arranged by size: 4545 543 534 91 84 81 73 47 BamHI G'GATC_C Cuts at: 3123 3328 3695 3123 Size: 205 367 5426 Fragments arranged by size: 5426 367 205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2212 2239 3319 4774 Size: 325 217 627 273 152 27 1080 1455 Cuts at: 4774 618 Size: 1842 Fragments arranged by size: 1842 1455 1080 627 325 273 217 152 27 BanII G_rGCy'C Cuts at: 1391 2873 3204 3317 1391 Size: 1482 331 113 4072 Fragments arranged by size: 4072 1482 331 113 BbeI G_GCGC'C Cuts at: 2243 2243 Size: 5998 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2416 3064 3166 3267 928 Size: 971 517 648 102 101 3659 Fragments arranged by size: 3659 971 648 517 102 101 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2254 2288 4189 4223 1903 Size: 34 317 34 1901 34 3678 Fragments arranged by size: 3678 1901 317 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 1548 Size: 5998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2831 4609 136 Size: 107 122 71 631 605 1159 1778 1525 Fragments arranged by size: 1778 1525 1159 631 605 122 107 71 BglII A'GATC_T Cuts at: 2559 5993 2559 Size: 3434 2564 BlpI GC'TnA_GC Cuts at: 3415 3415 Size: 5998 Bme1580I G_kGCm'C Cuts at: 1790 2217 2652 2873 4059 5215 1790 Size: 427 435 221 1186 1156 2573 Fragments arranged by size: 2573 1186 1156 435 427 221 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5998 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2639 3729 4687 Size: 416 53 83 399 24 1222 1090 958 Cuts at: 4687 442 Size: 1753 Fragments arranged by size: 1753 1222 1090 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2519 3026 3188 3245 4658 1239 Size: 1280 507 162 57 1413 2579 Fragments arranged by size: 2579 1413 1280 507 162 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2573 4123 4988 5286 2573 Size: 1550 865 298 3285 Fragments arranged by size: 3285 1550 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3134 4661 882 Size: 617 1635 1527 2219 Fragments arranged by size: 2219 1635 1527 617 BsaAI yAC'GTr Cuts at: 493 1204 3789 493 Size: 711 2585 2702 Fragments arranged by size: 2702 2585 711 BsaBI GATnn'nnATC Cuts at: 2558 5992 2558 Size: 3434 2564 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2240 2963 3805 4187 Size: 53 83 186 936 707 723 842 382 Cuts at: 4187 4822 275 Size: 635 1451 Fragments arranged by size: 1451 936 842 723 707 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2179 4431 4829 5103 5250 5661 2179 Size: 2252 398 274 147 411 2516 Fragments arranged by size: 2516 2252 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2528 2558 1178 Size: 30 165 30 1125 30 4618 Fragments arranged by size: 4618 1125 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2957 2984 3031 3242 1186 Size: 1771 27 47 211 3942 Fragments arranged by size: 3942 1771 211 47 27 BseYI C'CCAG_C Cuts at: 2010 2171 2784 3024 5201 5697 2010 Size: 161 613 240 2177 496 2311 Fragments arranged by size: 2311 2177 613 496 240 161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2303 2790 1163 Size: 573 567 487 4371 Fragments arranged by size: 4371 573 567 487 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 4209 5237 1540 Size: 275 186 1447 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2301 3097 3204 4059 4144 5215 2301 Size: 796 107 855 85 1071 3084 Fragments arranged by size: 3084 1071 855 796 107 85 BsmI GAATG_Cn' Cuts at: 3509 3602 3509 Size: 93 5905 BsmBI CGTCTCn'nnnn_ Cuts at: 2531 2954 2531 Size: 423 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2301 2652 2873 3097 3204 3317 Size: 399 427 84 351 221 224 107 113 Cuts at: 3317 4059 4144 5215 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 427 399 351 224 221 113 107 85 84 BspEI T'CCGG_A Cuts at: 5661 5661 Size: 5998 BspHI T'CATG_A Cuts at: 2625 3097 3887 5617 2625 Size: 472 790 1730 3006 Fragments arranged by size: 3006 1730 790 472 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2072 2667 3279 3353 844 Size: 1228 595 612 74 3489 Fragments arranged by size: 3489 1228 612 595 74 BsrBI CCG'CTC Cuts at: 1394 1823 2374 3885 1394 Size: 429 551 1511 3507 Fragments arranged by size: 3507 1511 551 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4493 4667 65 Size: 1183 3245 174 1396 Fragments arranged by size: 3245 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2109 4642 4829 1700 Size: 110 299 2533 187 2869 Fragments arranged by size: 2869 2533 299 187 110 BsrGI T'GTAC_A Cuts at: 96 2388 96 Size: 2292 3706 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 4058 5070 4058 Size: 1012 4986 BstAPI GCAn_nnn'nTGC Cuts at: 794 2281 2305 794 Size: 1487 24 4487 Fragments arranged by size: 4487 1487 24 BstBI TT'CG_AA Cuts at: 4802 4802 Size: 5998 BstEII G'GTnAC_C Cuts at: 1184 2728 1184 Size: 1544 4454 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2209 3085 2029 Size: 180 876 4942 Fragments arranged by size: 4942 876 180 BstYI r'GATC_y Cuts at: 1972 2559 2776 3123 3162 3328 3695 4080 4097 Size: 587 217 347 39 166 367 385 17 Cuts at: 4097 5549 5635 5647 5993 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 587 385 367 347 346 217 166 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2515 3168 513 Size: 552 1450 653 3343 Fragments arranged by size: 3343 1450 653 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5259 Fragments arranged by size: 5259 683 56 BtsI GCAGTG_nn' Cuts at: 2128 3514 4308 4328 2128 Size: 1386 794 20 3798 Fragments arranged by size: 3798 1386 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5963 DraI TTT'AAA Cuts at: 3302 3302 Size: 5998 DraIII CAC_nnn'GTG Cuts at: 2305 2521 2305 Size: 216 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2682 3445 4334 Size: 54 1425 50 275 18 852 763 889 Cuts at: 4334 5963 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 852 763 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2952 3928 1353 Size: 1599 976 3423 Fragments arranged by size: 3423 1599 976 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3167 4585 4959 5105 387 Size: 1064 138 1578 1418 374 146 1280 Fragments arranged by size: 1578 1418 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3202 3202 Size: 5998 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2519 2624 3026 3188 3245 4061 4658 Size: 393 887 105 402 162 57 816 597 Cuts at: 4658 5445 5713 1239 Size: 787 268 1524 Fragments arranged by size: 1524 887 816 787 597 402 393 268 162 105 57 EcoNI CCTnn'n_nnAGG Cuts at: 2663 3242 2663 Size: 579 5419 EcoO109I rG'GnC_Cy Cuts at: 2049 2101 2122 2224 2377 2656 2668 2869 2870 Size: 52 21 102 153 279 12 201 1 Cuts at: 2870 3065 3130 2049 Size: 195 65 4917 Fragments arranged by size: 4917 279 201 195 153 102 65 52 21 12 1 EcoRI G'AATT_C Cuts at: 3307 3685 3307 Size: 378 5620 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2284 2440 2284 Size: 156 5842 FspI TGC'GCA Cuts at: 2348 4504 2348 Size: 2156 3842 HaeII r_GCGC'y Cuts at: 1678 2243 5145 1678 Size: 565 2902 2531 Fragments arranged by size: 2902 2531 565 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2800 2832 4639 4671 4713 Size: 32 135 32 1228 32 1807 32 42 Cuts at: 4713 4745 1373 Size: 32 2626 Fragments arranged by size: 2626 1807 1228 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2635 3342 3588 669 Size: 988 281 697 707 246 3079 Fragments arranged by size: 3079 988 707 697 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5998 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KasI G'GCGC_C Cuts at: 2239 2239 Size: 5998 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5112 5296 1305 Size: 3807 184 2007 Fragments arranged by size: 3807 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2684 10 Size: 54 1425 343 852 3324 Fragments arranged by size: 3324 1425 852 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3083 3956 4315 4474 518 Size: 648 1917 873 359 159 2042 Fragments arranged by size: 2042 1917 873 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2141 2398 2998 3414 4091 5239 5484 1606 Size: 535 257 600 416 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 600 535 416 257 245 NaeI GCC'GGC Cuts at: 2111 2111 Size: 5998 NarI GG'CG_CC Cuts at: 2240 2240 Size: 5998 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5446 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NgoMIV G'CCGG_C Cuts at: 2109 2109 Size: 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 3356 4901 1132 Size: 97 2127 1545 2229 Fragments arranged by size: 2229 2127 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5998 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5096 PfoI T'CCnGG_A Cuts at: 2496 2496 Size: 5998 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2528 2560 4067 4099 5580 5612 2528 Size: 32 1507 32 1481 32 2914 Fragments arranged by size: 2914 1507 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2101 2224 2377 2656 2668 2101 Size: 123 153 279 12 5431 Fragments arranged by size: 5431 279 153 123 12 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PspOMI G'GGCC_C Cuts at: 2869 2869 Size: 5998 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2969 3001 2969 Size: 32 5966 PstI C_TGCA'G Cuts at: 830 2135 2277 3138 3220 3276 3350 830 Size: 1305 142 861 82 56 74 3478 Fragments arranged by size: 3478 1305 861 142 82 74 56 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 PvuII CAG'CTG Cuts at: 2141 2398 2998 2141 Size: 257 600 5141 Fragments arranged by size: 5141 600 257 SacI G_AGCT'C Cuts at: 3204 3204 Size: 5998 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SbfI CC_TGCA'GG Cuts at: 3138 3350 3138 Size: 212 5786 ScaI AGT'ACT Cuts at: 4246 5958 4246 Size: 1712 4286 SexAI A'CCwGG_T Cuts at: 2739 2739 Size: 5998 SfcI C'TryA_G Cuts at: 826 1045 2044 2131 2273 3134 3216 3272 3346 Size: 219 999 87 142 861 82 56 74 Cuts at: 3346 4481 5162 5353 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 999 861 681 219 191 142 87 82 74 56 SfiI GGCCn_nnn'nGGCC Cuts at: 2831 2831 Size: 5998 SfoI GGC'GCC Cuts at: 2241 2241 Size: 5998 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 2654 3197 3325 1872 Size: 782 543 128 4545 Fragments arranged by size: 4545 782 543 128 SmlI C'TyrA_G Cuts at: 820 1017 1943 2588 4102 5003 5265 820 Size: 197 926 645 1514 901 262 1553 Fragments arranged by size: 1553 1514 926 901 645 262 197 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3922 5 Size: 47 3870 2081 Fragments arranged by size: 3870 2081 47 StuI AGG'CCT Cuts at: 1517 2040 1517 Size: 523 5475 StyI C'CwwG_G Cuts at: 513 1065 2202 2415 3437 513 Size: 552 1137 213 1022 3074 Fragments arranged by size: 3074 1137 1022 552 213 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4025 4042 4195 4380 4825 325 Size: 1255 2445 17 153 185 445 1498 Fragments arranged by size: 2445 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2388 4244 5956 Size: 274 80 33 51 1443 411 1856 1712 Cuts at: 5956 96 Size: 138 Fragments arranged by size: 1856 1712 1443 411 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5958 5990 5958 Size: 32 5966 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2206 2590 3151 2206 Size: 384 561 5053 Fragments arranged by size: 5053 561 384 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5998 XmaI C'CCGG_G Cuts at: 1870 2652 3195 3323 1870 Size: 782 543 128 4545 Fragments arranged by size: 4545 782 543 128 XmnI GAAnn'nnTTC Cuts at: 4127 4127 Size: 5998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SapI SrfI SwaI