(Circular) (Six-base) MAPSORT of: pFN21AB6578.seq Check: 4592 from: 1 to: 5911 pFN21AB6578 5911 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2931 2931 Size: 5911 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2277 3721 278 Size: 53 83 186 936 741 1444 2468 Fragments arranged by size: 2468 1444 936 741 186 83 53 AccI GT'mk_AC Cuts at: 1937 3254 1937 Size: 1317 4594 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 4038 4411 1563 Size: 2475 373 3063 Fragments arranged by size: 3063 2475 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2749 2898 3974 5358 5626 1632 Size: 1117 149 1076 1384 268 1917 Fragments arranged by size: 1917 1384 1117 1076 268 149 AfeI AGC'GCT Cuts at: 2807 2807 Size: 5911 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5714 AflIII A'CryG_T Cuts at: 1203 4810 5787 1203 Size: 3607 977 1327 Fragments arranged by size: 3607 1327 977 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5911 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5911 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2319 2351 2319 Size: 32 5879 AlwNI CAG_nnn'CTG Cuts at: 2089 2949 3097 3654 5226 2089 Size: 860 148 557 1572 2774 Fragments arranged by size: 2774 1572 860 557 148 ApaI G_GGCC'C Cuts at: 2052 2422 3056 3162 2052 Size: 370 634 106 4801 Fragments arranged by size: 4801 634 370 106 ApaLI G'TGCA_C Cuts at: 2447 2626 3968 5124 2447 Size: 179 1342 1156 3234 Fragments arranged by size: 3234 1342 1156 179 ApoI r'AATT_y Cuts at: 1493 3220 3446 3598 5707 1493 Size: 1727 226 152 2109 1697 Fragments arranged by size: 2109 1727 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 2051 3154 3236 1870 Size: 73 108 1103 82 4545 Fragments arranged by size: 4545 1103 108 82 73 BamHI G'GATC_C Cuts at: 2587 3241 3608 2587 Size: 654 367 4890 Fragments arranged by size: 4890 654 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2163 3232 4687 618 Size: 325 217 627 376 1069 1455 1842 Fragments arranged by size: 1842 1455 1069 627 376 325 217 BanII G_rGCy'C Cuts at: 1391 2052 2422 2639 2859 3036 3056 3162 3230 Size: 661 370 217 220 177 20 106 68 Cuts at: 3230 1391 Size: 4072 Fragments arranged by size: 4072 661 370 220 217 177 106 68 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2974 928 Size: 971 1075 3865 Fragments arranged by size: 3865 1075 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4102 4136 1903 Size: 34 2165 34 3678 Fragments arranged by size: 3678 2165 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 5013 3803 Size: 1210 4701 BclI T'GATC_A Cuts at: 1548 2995 1548 Size: 1447 4464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2499 2958 3040 Size: 107 122 71 631 605 827 459 82 Cuts at: 3040 4522 136 Size: 1482 1525 Fragments arranged by size: 1525 1482 827 631 605 459 122 107 82 71 BglII A'GATC_T Cuts at: 5906 5906 Size: 5911 BlpI GC'TnA_GC Cuts at: 3328 3328 Size: 5911 Bme1580I G_kGCm'C Cuts at: 1790 2052 2166 2422 2451 2630 3056 3139 3162 Size: 262 114 256 29 179 426 83 23 Cuts at: 3162 3972 5128 1790 Size: 810 1156 2573 Fragments arranged by size: 2573 1156 810 426 262 256 179 114 83 29 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5911 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2461 3164 3642 Size: 416 53 83 399 24 1044 703 478 Cuts at: 3642 4600 442 Size: 958 1753 Fragments arranged by size: 1753 1044 958 703 478 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5911 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3133 3133 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2510 4571 1239 Size: 1271 2061 2579 Fragments arranged by size: 2579 2061 1271 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4036 4901 5199 4036 Size: 865 298 4748 Fragments arranged by size: 4748 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2682 4574 882 Size: 617 1183 1892 2219 Fragments arranged by size: 2219 1892 1183 617 BsaAI yAC'GTr Cuts at: 493 1204 3702 493 Size: 711 2498 2702 Fragments arranged by size: 2702 2498 711 BsaBI GATnn'nnATC Cuts at: 5905 5905 Size: 5911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2274 3718 4100 4735 Size: 53 83 186 936 741 1444 382 635 Cuts at: 4735 275 Size: 1451 Fragments arranged by size: 1451 1444 936 741 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4344 4742 5016 5163 5574 4344 Size: 398 274 147 411 4681 Fragments arranged by size: 4681 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2369 2399 1178 Size: 30 165 30 966 30 4690 Fragments arranged by size: 4690 966 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 2210 2371 2390 2426 1186 Size: 1012 12 161 19 36 4671 Fragments arranged by size: 4671 1012 161 36 19 12 BseYI C'CCAG_C Cuts at: 3055 3060 3064 5114 5610 3055 Size: 5 4 2050 496 3356 Fragments arranged by size: 3356 2050 496 5 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2337 2811 1163 Size: 573 601 474 4263 Fragments arranged by size: 4263 601 573 474 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2152 3361 4122 5150 1540 Size: 275 186 151 1209 761 1028 2301 Fragments arranged by size: 2301 1209 1028 761 275 186 151 BsiHKAI G_wGCw'C Cuts at: 2451 2630 3132 3972 4057 5128 2451 Size: 179 502 840 85 1071 3234 Fragments arranged by size: 3234 1071 840 502 179 85 BsmI GAATG_Cn' Cuts at: 3422 3515 3422 Size: 93 5818 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2166 2422 2451 2630 2639 2859 Size: 399 262 114 256 29 179 9 220 Cuts at: 2859 3036 3056 3132 3139 3162 3230 3972 4057 Size: 177 20 76 7 23 68 742 85 Cuts at: 4057 5128 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 262 256 220 179 177 114 85 76 68 29 23 20 9 7 BspEI T'CCGG_A Cuts at: 5574 5574 Size: 5911 BspHI T'CATG_A Cuts at: 3800 5530 3800 Size: 1730 4181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2931 3076 3266 844 Size: 2087 145 190 3489 Fragments arranged by size: 3489 2087 190 145 BsrBI CCG'CTC Cuts at: 1394 1823 2416 3798 1394 Size: 429 593 1382 3507 Fragments arranged by size: 3507 1382 593 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4406 4580 65 Size: 1183 3158 174 1396 Fragments arranged by size: 3158 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4555 4742 1700 Size: 110 2745 187 2869 Fragments arranged by size: 2869 2745 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5911 BssHII G'CGCG_C Cuts at: 1925 2188 1925 Size: 263 5648 BssSI C'ACGA_G Cuts at: 3029 3971 4983 3029 Size: 942 1012 3957 Fragments arranged by size: 3957 1012 942 BstAPI GCAn_nnn'nTGC Cuts at: 794 2949 794 Size: 2155 3756 BstBI TT'CG_AA Cuts at: 4715 4715 Size: 5911 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5911 BstXI CCAn_nnnn'nTGG Cuts at: 2266 2830 2266 Size: 564 5347 BstYI r'GATC_y Cuts at: 1972 2587 3241 3608 3993 4010 5462 5548 5560 Size: 615 654 367 385 17 1452 86 12 Cuts at: 5560 5906 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 654 615 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5359 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5172 Fragments arranged by size: 5172 683 56 BtsI GCAGTG_nn' Cuts at: 2307 2950 3427 4221 4241 2307 Size: 643 477 794 20 3977 Fragments arranged by size: 3977 794 643 477 20 ClaI AT'CG_AT Cuts at: 1554 2706 1554 Size: 1152 4759 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5876 DraI TTT'AAA Cuts at: 3215 3215 Size: 5911 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4918 809 Size: 507 3602 1802 Fragments arranged by size: 3602 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 2310 3183 Size: 54 1425 50 275 18 250 230 873 Cuts at: 3183 3358 4247 5876 8 Size: 175 889 1629 43 Fragments arranged by size: 1629 1425 889 873 275 250 230 175 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3358 1537 Size: 275 1546 4090 Fragments arranged by size: 4090 1546 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2403 3107 3170 3841 1353 Size: 1050 704 63 671 3423 Fragments arranged by size: 3423 1050 704 671 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 1999 2162 2195 2285 4498 4872 Size: 1064 138 410 163 33 90 2213 374 Cuts at: 4872 5018 387 Size: 146 1280 Fragments arranged by size: 2213 1280 1064 410 374 163 146 138 90 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2510 2749 2898 3974 4571 5358 5626 Size: 393 878 239 149 1076 597 787 268 Cuts at: 5626 1239 Size: 1524 Fragments arranged by size: 1524 1076 878 787 597 393 268 239 149 EcoO109I rG'GnC_Cy Cuts at: 3052 3158 3159 3052 Size: 106 1 5804 Fragments arranged by size: 5804 106 1 EcoRI G'AATT_C Cuts at: 3220 3598 3220 Size: 378 5533 FspI TGC'GCA Cuts at: 4417 4417 Size: 5911 HaeII r_GCGC'y Cuts at: 1678 2465 2809 5058 1678 Size: 787 344 2249 2531 Fragments arranged by size: 2531 2249 787 344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4552 4584 4626 4658 1373 Size: 32 135 32 2980 32 42 32 2626 Fragments arranged by size: 2980 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3255 3501 669 Size: 988 281 1317 246 3079 Fragments arranged by size: 3079 1317 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5911 HpaI GTT'AAC Cuts at: 3501 3501 Size: 5911 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 2735 3510 2735 Size: 775 5136 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2580 5025 5209 1305 Size: 1275 2445 184 2007 Fragments arranged by size: 2445 2007 1275 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2312 10 Size: 54 1425 343 480 3609 Fragments arranged by size: 3609 1425 480 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2517 3869 4228 4387 518 Size: 648 1351 1352 359 159 2042 Fragments arranged by size: 2042 1352 1351 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2341 2383 2443 2530 3064 3327 4004 5152 Size: 735 42 60 87 534 263 677 1148 Cuts at: 5152 5397 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 735 677 534 263 245 87 60 42 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5359 NdeI CA'TA_TG Cuts at: 387 387 Size: 5911 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5911 NotI GC'GGCC_GC Cuts at: 3358 3358 Size: 5911 NspI r_CATG'y Cuts at: 1132 1229 2506 3269 4814 1132 Size: 97 1277 763 1545 2229 Fragments arranged by size: 2229 1545 1277 763 97 PasI CC'CwG_GG Cuts at: 1348 3037 1348 Size: 1689 4222 PciI A'CATG_T Cuts at: 4810 4810 Size: 5911 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5911 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3980 4012 5493 5525 3980 Size: 32 1481 32 4366 Fragments arranged by size: 4366 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2952 1613 Size: 1339 4572 PsiI TTA'TAA Cuts at: 3481 3481 Size: 5911 PspOMI G'GGCC_C Cuts at: 2048 2418 3052 3158 2048 Size: 370 634 106 4801 Fragments arranged by size: 4801 634 370 106 PstI C_TGCA'G Cuts at: 830 2238 2469 3263 830 Size: 1408 231 794 3478 Fragments arranged by size: 3478 1408 794 231 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 PvuII CAG'CTG Cuts at: 2530 3064 2530 Size: 534 5377 SalI G'TCGA_C Cuts at: 1936 3253 1936 Size: 1317 4594 SapI GCTCTTCn'nnn_ Cuts at: 3107 3107 Size: 5911 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SfcI C'TryA_G Cuts at: 826 1045 2234 2465 3259 4394 5075 5266 826 Size: 219 1189 231 794 1135 681 191 1471 Fragments arranged by size: 1471 1189 1135 794 681 231 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5911 SmaI CCC'GGG Cuts at: 1872 2053 3156 3238 1872 Size: 181 1103 82 4545 Fragments arranged by size: 4545 1103 181 82 SmlI C'TyrA_G Cuts at: 820 1017 1943 4015 4916 5178 820 Size: 197 926 2072 901 262 1553 Fragments arranged by size: 2072 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 2506 3269 1132 Size: 1374 763 3774 Fragments arranged by size: 3774 1374 763 SrfI GCCC'GGGC Cuts at: 2053 2053 Size: 5911 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 2786 1517 Size: 1269 4642 StyI C'CwwG_G Cuts at: 513 1065 3350 513 Size: 552 2285 3074 Fragments arranged by size: 3074 2285 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2579 3056 3938 3955 4108 4293 4738 Size: 1255 999 477 882 17 153 185 445 Cuts at: 4738 325 Size: 1498 Fragments arranged by size: 1498 1255 999 882 477 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4157 5869 96 Size: 274 80 33 51 1443 2180 1712 138 Fragments arranged by size: 2180 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5871 5903 5871 Size: 32 5879 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5911 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5911 XmaI C'CCGG_G Cuts at: 1870 2051 3154 3236 1870 Size: 181 1103 82 4545 Fragments arranged by size: 4545 1103 181 82 XmnI GAAnn'nnTTC Cuts at: 4040 4040 Size: 5911 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2275 3719 276 Size: 53 83 186 936 741 1444 2468 Fragments arranged by size: 2468 1444 936 741 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SwaI XcmI