Restriction map for
pFN21AB6566
(Circular) (Six-base) MAPSORT of: pFN21AB6566.seq Check: 9689 from: 1 to: 5905
pFN21AB6566 5905 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 2349 3226 2349
Size: 877 5028
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2645 2666 2826 2850 2897 3968 5352 5620
Size: 1013 21 160 24 47 1071 1384 268
Cuts at: 5620 1632
Size: 1917
Fragments arranged by size:
1917 1384 1071 1013 268 160 47 24
21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 4804 5781 1203
Size: 3601 977 1327
Fragments arranged by size:
3601 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 3112 4634 3112
Size: 1522 4383
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3150 1184
Size: 1966 3939
AlwNI CAG_nnn'CTG
Cuts at: 2413 2527 3025 3648 5220 2413
Size: 114 498 623 1572 3098
Fragments arranged by size:
3098 1572 623 498 114
ApaI G_GGCC'C
Cuts at: 2373 2373
Size: 5905
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 3214 3440 3592 5701 1493
Size: 1721 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 2176 3155 3230 1870
Size: 73 233 979 75 4545
Fragments arranged by size:
4545 979 233 75 73
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2349 2432 2729 3226 4681
Size: 325 217 627 562 83 297 497 1455
Cuts at: 4681 618
Size: 1842
Fragments arranged by size:
1842 1455 627 562 497 325 297 217
83
BanII G_rGCy'C
Cuts at: 1391 2123 2373 3004 3224 1391
Size: 732 250 631 220 4072
Fragments arranged by size:
4072 732 631 250 220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2973 928
Size: 971 1074 3860
Fragments arranged by size:
3860 1074 971
BbvCI CC'TCA_GC
Cuts at: 2678 2678
Size: 5905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2719 2753 3136 3170 4096 4130 1903
Size: 34 782 34 383 34 926 34 3678
Fragments arranged by size:
3678 926 782 383 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2149 3797 5007 2149
Size: 1648 1210 3047
Fragments arranged by size:
3047 1648 1210
BclI T'GATC_A
Cuts at: 1548 3027 1548
Size: 1479 4426
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2061 2309 2483
Size: 107 122 71 631 605 389 248 174
Cuts at: 2483 3008 4516 136
Size: 525 1508 1525
Fragments arranged by size:
1525 1508 631 605 525 389 248 174
122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 2945 3322 2945
Size: 377 5528
Bme1580I G_kGCm'C
Cuts at: 1790 2373 2732 3966 5122 1790
Size: 583 359 1234 1156 2573
Fragments arranged by size:
2573 1234 1156 583 359
BmgBI CAC'GTC
Cuts at: 1318 2040 1318
Size: 722 5183
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3636 4594 442
Size: 416 53 83 399 24 2219 958 1753
Fragments arranged by size:
2219 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2434 2624 2855 3143 4565 1239
Size: 1088 107 190 231 288 1422 2579
Fragments arranged by size:
2579 1422 1088 288 231 190 107
Bpu10I CC'TnA_GC
Cuts at: 2678 2678
Size: 5905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2942 4030 4895 5193 2942
Size: 1088 865 298 3654
Fragments arranged by size:
3654 1088 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 2612 5899 2612
Size: 3287 2618
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2205 3712 4094 4729
Size: 53 83 186 936 672 1507 382 635
Cuts at: 4729 275
Size: 1451
Fragments arranged by size:
1507 1451 936 672 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2793 4338 4736 5010 5157 5568 2793
Size: 1545 398 274 147 411 3130
Fragments arranged by size:
3130 1545 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2105 2135 2685 2715 1178
Size: 30 165 30 702 30 550 30 4368
Fragments arranged by size:
4368 702 550 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2164 2675 1186
Size: 978 511 4416
Fragments arranged by size:
4416 978 511
BseYI C'CCAG_C
Cuts at: 2105 3003 3045 3049 5108 5604 2105
Size: 898 42 4 2059 496 2406
Fragments arranged by size:
2406 2059 898 496 42 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5332
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2131 2272 3355 4116 5144 1540
Size: 275 186 130 141 1083 761 1028 2301
Fragments arranged by size:
2301 1083 1028 761 275 186 141 130
BsiHKAI G_wGCw'C
Cuts at: 3966 4051 5122 3966
Size: 85 1071 4749
Fragments arranged by size:
4749 1071 85
BsmI GAATG_Cn'
Cuts at: 2109 3416 3509 2109
Size: 1307 93 4505
Fragments arranged by size:
4505 1307 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2507 2507
Size: 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2373 2732 3004 3224 3966 4051
Size: 399 333 250 359 272 220 742 85
Cuts at: 4051 5122 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 359 333 272 250
220 85
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2421 2871 2907 3260 844
Size: 1577 450 36 353 3489
Fragments arranged by size:
3489 1577 450 353 36
BsrBI CCG'CTC
Cuts at: 1394 1823 2723 3076 3792 1394
Size: 429 900 353 716 3507
Fragments arranged by size:
3507 900 716 429 353
BsrDI GCAATG_nn'
Cuts at: 65 1248 4400 4574 65
Size: 1183 3152 174 1396
Fragments arranged by size:
3152 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4549 4736 1700
Size: 110 2739 187 2869
Fragments arranged by size:
2869 2739 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 2100 2299 2990 1925
Size: 175 199 691 4840
Fragments arranged by size:
4840 691 199 175
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2337 2745 3235 3602 3987 4004 5456 5542
Size: 365 408 490 367 385 17 1452 86
Cuts at: 5542 5554 5900 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 490 408 385 367 365 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2131 513
Size: 552 1066 4287
Fragments arranged by size:
4287 1066 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3069 487
Size: 683 56 1843 3323
Fragments arranged by size:
3323 1843 683 56
BtsI GCAGTG_nn'
Cuts at: 3421 4215 4235 3421
Size: 794 20 5091
Fragments arranged by size:
5091 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2128 2313
Size: 54 1425 50 275 18 204 94 185
Cuts at: 2313 2589 2781 2854 3352 4241 5870 8
Size: 276 192 73 498 889 1629 43
Fragments arranged by size:
1629 1425 889 498 276 275 204 192
185 94 73 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2128 3352 1537
Size: 275 316 1224 4090
Fragments arranged by size:
4090 1224 316 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2625 3835 1353
Size: 1272 1210 3423
Fragments arranged by size:
3423 1272 1210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2140 4492 4866 5012 387
Size: 1064 138 551 2352 374 146 1280
Fragments arranged by size:
2352 1280 1064 551 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2434 2624 2645 2666 2826 2850
Size: 393 695 107 190 21 21 160 24
Cuts at: 2850 2855 2897 3143 3968 4565 5352 5620 1239
Size: 5 42 246 825 597 787 268 1524
Fragments arranged by size:
1524 825 787 695 597 393 268 246
190 160 107 42 24 21 21 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2291 3155 2291
Size: 864 5041
EcoO109I rG'GnC_Cy
Cuts at: 2181 2363 2370 2410 3199 2181
Size: 182 7 40 789 4887
Fragments arranged by size:
4887 789 182 40 7
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FspI TGC'GCA
Cuts at: 2531 2564 4411 2531
Size: 33 1847 4025
Fragments arranged by size:
4025 1847 33
HaeII r_GCGC'y
Cuts at: 1678 3026 5052 1678
Size: 1348 2026 2531
Fragments arranged by size:
2531 2026 1348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2685 2717 2921 2953 3098
Size: 32 135 32 1113 32 204 32 145
Cuts at: 3098 3130 4546 4578 4620 4652 1373
Size: 32 1416 32 42 32 2626
Fragments arranged by size:
2626 1416 1113 204 145 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3249 3495 669
Size: 988 281 1311 246 3079
Fragments arranged by size:
3079 1311 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KpnI G_GTAC'C
Cuts at: 2353 3230 2353
Size: 877 5028
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 5019 5203 1305
Size: 939 2775 184 2007
Fragments arranged by size:
2775 2007 939 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2591 2856 10
Size: 54 1425 343 759 265 3059
Fragments arranged by size:
3059 1425 759 343 265 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2537 3863 4222 4381 518
Size: 648 1371 1326 359 159 2042
Fragments arranged by size:
2042 1371 1326 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2133 2944 3007 3033 3049 3097 3321 3998
Size: 527 811 63 26 16 48 224 677
Cuts at: 3998 5146 5391 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 811 677 527 245 224 63
48 26 16
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5353
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 2128 3352 2128
Size: 1224 4681
NspI r_CATG'y
Cuts at: 1132 1229 2761 3263 4808 1132
Size: 97 1532 502 1545 2229
Fragments arranged by size:
2229 1545 1532 502 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PfoI T'CCnGG_A
Cuts at: 2406 2450 2406
Size: 44 5861
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2410 3199 2410
Size: 789 5116
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2369 2369
Size: 5905
PspXI vC'TCGA_Gb
Cuts at: 3155 3155
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2466 2491 2937 3257 830
Size: 1636 25 446 320 3478
Fragments arranged by size:
3478 1636 446 320 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 2944 3007 3049 2944
Size: 63 42 5800
Fragments arranged by size:
5800 63 42
SacII CC_GC'GG
Cuts at: 2134 2134
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SapI GCTCTTCn'nnn_
Cuts at: 2625 2625
Size: 5905
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2014 2383 2462 2487 2933 3253 4388
Size: 219 969 369 79 25 446 320 1135
Cuts at: 4388 5069 5260 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 969 681 446 369 320 219
191 79 25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2921 3155 4009 4910 5172 820
Size: 197 926 978 234 854 901 262 1553
Fragments arranged by size:
1553 978 926 901 854 262 234 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 2055 2622 1517
Size: 538 567 4800
Fragments arranged by size:
4800 567 538
StyI C'CwwG_G
Cuts at: 513 1065 2961 3195 3344 513
Size: 552 1896 234 149 3074
Fragments arranged by size:
3074 1896 552 234 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2612 3932 3949 4102 4287 4732 325
Size: 1255 1032 1320 17 153 185 445 1498
Fragments arranged by size:
1498 1320 1255 1032 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4151 5863 96
Size: 274 80 33 51 1443 2174 1712 138
Fragments arranged by size:
2174 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2105 2137 5865 5897 2105
Size: 32 3728 32 2113
Fragments arranged by size:
3728 2113 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2215 1316
Size: 899 5006
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2599 2599
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 3155 1943
Size: 1212 4693
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PsrI RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI