Restriction map for
pFN21AB6566
(Linear) (Six-base) MAPSORT of: KIBB6566.seq Check: 5573 from: 1 to: 1200
KIBB6566.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
Acc65I G'GTAC_C
Cuts at: 0 344 1200
Size: 344 856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 661 821 845 892 1200
Size: 640 21 160 24 47 308
Fragments arranged by size:
640 308 160 47 24 21
AhdI GACnn_n'nnGTC
Cuts at: 0 1107 1200
Size: 1107 93
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1145 1200
Size: 1145 55
AlwNI CAG_nnn'CTG
Cuts at: 0 408 522 1020 1200
Size: 408 114 498 180
Fragments arranged by size:
498 408 180 114
ApaI G_GGCC'C
Cuts at: 0 368 1200
Size: 368 832
AvaI C'yCGr_G
Cuts at: 0 171 1150 1200
Size: 171 979 50
Fragments arranged by size:
979 171 50
BanI G'GyrC_C
Cuts at: 0 344 427 724 1200
Size: 344 83 297 476
Fragments arranged by size:
476 344 297 83
BanII G_rGCy'C
Cuts at: 0 118 368 999 1200
Size: 118 250 631 201
Fragments arranged by size:
631 250 201 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 968 1200
Size: 968 232
BbvCI CC'TCA_GC
Cuts at: 0 673 1200
Size: 673 527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 714 748 1131 1165 1200
Size: 714 34 383 34 35
Fragments arranged by size:
714 383 35 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 144 1200
Size: 144 1056
BclI T'GATC_A
Cuts at: 0 1022 1200
Size: 1022 178
BglI GCCn_nnn'nGGC
Cuts at: 0 56 304 478 1003 1200
Size: 56 248 174 525 197
Fragments arranged by size:
525 248 197 174 56
BlpI GC'TnA_GC
Cuts at: 0 940 1200
Size: 940 260
Bme1580I G_kGCm'C
Cuts at: 0 368 727 1200
Size: 368 359 473
Fragments arranged by size:
473 368 359
BmgBI CAC'GTC
Cuts at: 0 35 1200
Size: 35 1165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 429 619 850 1138 1200
Size: 322 107 190 231 288 62
Fragments arranged by size:
322 288 231 190 107 62
Bpu10I CC'TnA_GC
Cuts at: 0 673 1200
Size: 673 527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1200
Size: 937 263
BsaBI GATnn'nnATC
Cuts at: 0 607 1200
Size: 607 593
BsaHI Gr'CG_yC
Cuts at: 0 200 1200
Size: 200 1000
BsaWI w'CCGG_w
Cuts at: 0 788 1200
Size: 788 412
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 100 130 680 710 1200
Size: 100 30 550 30 490
Fragments arranged by size:
550 490 100 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 159 670 1200
Size: 159 511 530
Fragments arranged by size:
530 511 159
BseYI C'CCAG_C
Cuts at: 0 100 998 1040 1044 1200
Size: 100 898 42 4 156
Fragments arranged by size:
898 156 100 42 4
BsiEI CG_ry'CG
Cuts at: 0 126 267 1200
Size: 126 141 933
Fragments arranged by size:
933 141 126
BsmI GAATG_Cn'
Cuts at: 0 104 1200
Size: 104 1096
BsmBI CGTCTCn'nnnn_
Cuts at: 0 502 1200
Size: 502 698
Bsp1286I G_dGCh'C
Cuts at: 0 118 368 727 999 1200
Size: 118 250 359 272 201
Fragments arranged by size:
359 272 250 201 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 416 866 902 1200
Size: 416 450 36 298
Fragments arranged by size:
450 416 298 36
BsrBI CCG'CTC
Cuts at: 0 718 1071 1200
Size: 718 353 129
Fragments arranged by size:
718 353 129
BssHII G'CGCG_C
Cuts at: 0 95 294 985 1200
Size: 95 199 691 215
Fragments arranged by size:
691 215 199 95
BstYI r'GATC_y
Cuts at: 0 332 740 1200
Size: 332 408 460
Fragments arranged by size:
460 408 332
BtgI C'CryG_G
Cuts at: 0 126 1200
Size: 126 1074
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1064 1200
Size: 1064 136
EaeI y'GGCC_r
Cuts at: 0 29 123 308 584 776 849 1200
Size: 29 94 185 276 192 73 351
Fragments arranged by size:
351 276 192 185 94 73 29
EagI C'GGCC_G
Cuts at: 0 123 1200
Size: 123 1077
EarI CTCTTCn'nnn_
Cuts at: 0 620 1200
Size: 620 580
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 135 1200
Size: 135 1065
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 429 619 640 661 821 845 850
Size: 322 107 190 21 21 160 24 5
Cuts at: 850 892 1138 1200
Size: 42 246 62
Fragments arranged by size:
322 246 190 160 107 62 42 24
21 21 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 286 1150 1200
Size: 286 864 50
Fragments arranged by size:
864 286 50
EcoO109I rG'GnC_Cy
Cuts at: 0 176 358 365 405 1194 1200
Size: 176 182 7 40 789 6
Fragments arranged by size:
789 182 176 40 7 6
FspI TGC'GCA
Cuts at: 0 526 559 1200
Size: 526 33 641
Fragments arranged by size:
641 526 33
HaeII r_GCGC'y
Cuts at: 0 1021 1200
Size: 1021 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 680 712 916 948 1093 1125 1200
Size: 680 32 204 32 145 32 75
Fragments arranged by size:
680 204 145 75 32 32 32
KpnI G_GTAC'C
Cuts at: 0 348 1200
Size: 348 852
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1200
Size: 239 961
MscI TGG'CCA
Cuts at: 0 586 851 1200
Size: 586 265 349
Fragments arranged by size:
586 349 265
MslI CAynn'nnrTG
Cuts at: 0 532 1200
Size: 532 668
MspA1I CmG'CkG
Cuts at: 0 128 939 1002 1028 1044 1092 1200
Size: 128 811 63 26 16 48 108
Fragments arranged by size:
811 128 108 63 48 26 16
NotI GC'GGCC_GC
Cuts at: 0 123 1200
Size: 123 1077
NspI r_CATG'y
Cuts at: 0 756 1200
Size: 756 444
PfoI T'CCnGG_A
Cuts at: 0 401 445 1200
Size: 401 44 755
Fragments arranged by size:
755 401 44
PpuMI rG'GwC_Cy
Cuts at: 0 405 1194 1200
Size: 405 789 6
Fragments arranged by size:
789 405 6
PspOMI G'GGCC_C
Cuts at: 0 364 1200
Size: 364 836
PspXI vC'TCGA_Gb
Cuts at: 0 1150 1200
Size: 1150 50
PstI C_TGCA'G
Cuts at: 0 461 486 932 1200
Size: 461 25 446 268
Fragments arranged by size:
461 446 268 25
PvuII CAG'CTG
Cuts at: 0 939 1002 1044 1200
Size: 939 63 42 156
Fragments arranged by size:
939 156 63 42
SacII CC_GC'GG
Cuts at: 0 129 1200
Size: 129 1071
SapI GCTCTTCn'nnn_
Cuts at: 0 620 1200
Size: 620 580
SfcI C'TryA_G
Cuts at: 0 9 378 457 482 928 1200
Size: 9 369 79 25 446 272
Fragments arranged by size:
446 369 272 79 25 9
SmlI C'TyrA_G
Cuts at: 0 916 1150 1200
Size: 916 234 50
Fragments arranged by size:
916 234 50
StuI AGG'CCT
Cuts at: 0 50 617 1200
Size: 50 567 583
Fragments arranged by size:
583 567 50
StyI C'CwwG_G
Cuts at: 0 956 1190 1200
Size: 956 234 10
Fragments arranged by size:
956 234 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 607 1200
Size: 607 593
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 100 132 1200
Size: 100 32 1068
Fragments arranged by size:
1068 100 32
Tth111I GACn'n_nGTC
Cuts at: 0 210 1200
Size: 210 990
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 594 1200
Size: 594 606
XhoI C'TCGA_G
Cuts at: 0 1150 1200
Size: 1150 50
Enzymes that do cut:
Acc65I AcuI AhdI AlfI AlwNI ApaI AvaI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsmI BsmBI Bsp1286I BspMI
BsrBI BssHII BstYI BtgI BtgZI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KpnI
MmeI MscI MslI MspA1I NotI NspI PfoI PpuMI
PspOMI PspXI PstI PvuII SacII SapI SfcI SmlI
StuI StyI TaqII TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BfrBI BglII BmrI BmtI BplI
BsaI BsaAI BsgI BsiHKAI BsiWI BspEI BspHI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoICRI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacI
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI XbaI XmaI XmnI ZraI