(Circular) (Six-base) MAPSORT of: pFN21AB6676.seq Check: 2602 from: 1 to: 6067 pFN21AB6676 6067 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3877 278 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3410 1937 Size: 1473 4594 Acc65I G'GTAC_C Cuts at: 3388 3388 Size: 6067 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2379 4130 5514 5782 1632 Size: 747 1751 1384 268 1917 Fragments arranged by size: 1917 1751 1384 747 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 4966 5943 1203 Size: 3763 977 1327 Fragments arranged by size: 3763 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6067 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3116 1184 Size: 1932 4135 AlwNI CAG_nnn'CTG Cuts at: 2020 2083 3810 5382 2020 Size: 63 1727 1572 2705 Fragments arranged by size: 2705 1727 1572 63 ApaI G_GGCC'C Cuts at: 2311 2797 2311 Size: 486 5581 ApaLI G'TGCA_C Cuts at: 4124 5280 4124 Size: 1156 4911 ApoI r'AATT_y Cuts at: 1493 2363 3054 3376 3602 3754 5863 1493 Size: 870 691 322 226 152 2109 1697 Fragments arranged by size: 2109 1697 870 691 322 226 152 AseI AT'TA_AT Cuts at: 160 1032 4621 160 Size: 872 3589 1606 Fragments arranged by size: 3589 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 2789 3392 1870 Size: 73 846 603 4545 Fragments arranged by size: 4545 846 603 73 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3388 4843 618 Size: 325 217 627 1601 1455 1842 Fragments arranged by size: 1842 1601 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2061 2311 2343 2797 3309 3386 1391 Size: 670 250 32 454 512 77 4072 Fragments arranged by size: 4072 670 512 454 250 77 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2694 2775 928 Size: 971 795 81 4220 Fragments arranged by size: 4220 971 795 81 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3182 3216 4258 4292 1903 Size: 34 50 34 1161 34 1042 34 3678 Fragments arranged by size: 3678 1161 1042 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3959 5169 3959 Size: 1210 4857 BclI T'GATC_A Cuts at: 1548 1548 Size: 6067 BfrBI ATG'CAT Cuts at: 2588 2588 Size: 6067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3251 4678 136 Size: 107 122 71 631 605 1579 1427 1525 Fragments arranged by size: 1579 1525 1427 631 605 122 107 71 BglII A'GATC_T Cuts at: 6062 6062 Size: 6067 BlpI GC'TnA_GC Cuts at: 2060 3484 2060 Size: 1424 4643 Bme1580I G_kGCm'C Cuts at: 1790 2311 2797 4128 5284 1790 Size: 521 486 1331 1156 2573 Fragments arranged by size: 2573 1331 1156 521 486 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3798 4756 442 Size: 416 53 83 399 24 2381 958 1753 Fragments arranged by size: 2381 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2489 2509 3374 4727 1239 Size: 1250 20 865 1353 2579 Fragments arranged by size: 2579 1353 1250 865 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3050 4192 5057 5355 3050 Size: 1142 865 298 3762 Fragments arranged by size: 3762 1142 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4730 882 Size: 617 3231 2219 Fragments arranged by size: 3231 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3858 493 Size: 711 2654 2702 Fragments arranged by size: 2702 2654 711 BsaBI GATnn'nnATC Cuts at: 2043 6061 2043 Size: 4018 2049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3207 3874 4256 4891 Size: 53 83 186 936 1674 667 382 635 Cuts at: 4891 275 Size: 1451 Fragments arranged by size: 1674 1451 936 667 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2413 2973 4500 4898 5172 5319 5730 2413 Size: 560 1527 398 274 147 411 2750 Fragments arranged by size: 2750 1527 560 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5842 Fragments arranged by size: 5842 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3002 1186 Size: 1816 4251 BseYI C'CCAG_C Cuts at: 5270 5766 5270 Size: 496 5571 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5494 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2061 2283 3095 3309 4128 4213 5284 2061 Size: 222 812 214 819 85 1071 2844 Fragments arranged by size: 2844 1071 819 812 222 214 85 BsmI GAATG_Cn' Cuts at: 3578 3671 3578 Size: 93 5974 BsmBI CGTCTCn'nnnn_ Cuts at: 2734 2734 Size: 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2283 2311 2343 2797 3095 3309 Size: 399 271 222 28 32 454 298 214 Cuts at: 3309 3386 4128 4213 5284 1391 Size: 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 454 399 298 271 222 214 85 77 32 28 BspEI T'CCGG_A Cuts at: 2413 5730 2413 Size: 3317 2750 BspHI T'CATG_A Cuts at: 3956 5686 3956 Size: 1730 4337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3422 844 Size: 2578 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3008 3954 1394 Size: 429 1185 946 3507 Fragments arranged by size: 3507 1185 946 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4562 4736 65 Size: 1183 3314 174 1396 Fragments arranged by size: 3314 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4711 4898 1700 Size: 110 2901 187 2869 Fragments arranged by size: 2901 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2031 96 Size: 1935 4132 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6067 BssSI C'ACGA_G Cuts at: 2763 4127 5139 2763 Size: 1364 1012 3691 Fragments arranged by size: 3691 1364 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6067 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6067 BstYI r'GATC_y Cuts at: 1972 2038 2079 2109 3397 3764 4149 4166 5618 Size: 66 41 30 1288 367 385 17 1452 Cuts at: 5618 5704 5716 6062 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1288 385 367 346 86 66 41 30 17 12 BtgI C'CryG_G Cuts at: 513 1065 3049 513 Size: 552 1984 3531 Fragments arranged by size: 3531 1984 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 3583 4377 4397 3583 Size: 794 20 5253 Fragments arranged by size: 5253 794 20 ClaI AT'CG_AT Cuts at: 1554 3192 1554 Size: 1638 4429 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 2983 3371 2983 Size: 388 5679 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3514 4403 6032 Size: 54 1425 50 275 18 1684 889 1629 Cuts at: 6032 8 Size: 43 Fragments arranged by size: 1684 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2331 2499 2646 2732 2800 3997 1353 Size: 978 168 147 86 68 1197 3423 Fragments arranged by size: 3423 1197 978 168 147 86 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3269 4654 5028 5174 387 Size: 1064 138 1680 1385 374 146 1280 Fragments arranged by size: 1680 1385 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2059 3307 2059 Size: 1248 4819 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 2489 2509 3374 4130 4727 5514 Size: 393 747 110 20 865 756 597 787 Cuts at: 5514 5782 1239 Size: 268 1524 Fragments arranged by size: 1524 865 787 756 747 597 393 268 110 20 EcoNI CCTnn'n_nnAGG Cuts at: 3299 3299 Size: 6067 EcoO109I rG'GnC_Cy Cuts at: 2307 2793 2307 Size: 486 5581 EcoRI G'AATT_C Cuts at: 2363 3054 3376 3754 2363 Size: 691 322 378 4676 Fragments arranged by size: 4676 691 378 322 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 5214 1678 Size: 3536 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2462 2494 4708 4740 4782 Size: 32 135 32 890 32 2214 32 42 Cuts at: 4782 4814 1373 Size: 32 2626 Fragments arranged by size: 2626 2214 890 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3411 3657 669 Size: 988 281 1473 246 3079 Fragments arranged by size: 3079 1473 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6067 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KpnI G_GTAC'C Cuts at: 3392 3392 Size: 6067 MfeI C'AATT_G Cuts at: 3666 3666 Size: 6067 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2004 2137 3144 5181 5365 1305 Size: 699 133 1007 2037 184 2007 Fragments arranged by size: 2037 2007 1007 699 184 133 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4245 Fragments arranged by size: 4245 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3098 4025 4384 4543 518 Size: 648 1932 927 359 159 2042 Fragments arranged by size: 2042 1932 927 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2729 3340 3483 4160 5308 5553 1606 Size: 1123 611 143 677 1148 245 2120 Fragments arranged by size: 2120 1148 1123 677 611 245 143 NcoI C'CATG_G Cuts at: 513 1065 3049 513 Size: 552 1984 3531 Fragments arranged by size: 3531 1984 552 NdeI CA'TA_TG Cuts at: 387 2584 387 Size: 2197 3870 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NsiI A_TGCA'T Cuts at: 2590 2590 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 3103 3245 3425 4970 1132 Size: 97 1874 142 180 1545 2229 Fragments arranged by size: 2229 1874 1545 180 142 97 PasI CC'CwG_GG Cuts at: 1348 3295 1348 Size: 1947 4120 PciI A'CATG_T Cuts at: 4966 4966 Size: 6067 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6067 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6067 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PspOMI G'GGCC_C Cuts at: 2307 2793 2307 Size: 486 5581 PstI C_TGCA'G Cuts at: 830 2409 3205 3419 830 Size: 1579 796 214 3478 Fragments arranged by size: 3478 1579 796 214 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2729 3340 2729 Size: 611 5456 SacI G_AGCT'C Cuts at: 2061 3309 2061 Size: 1248 4819 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SapI GCTCTTCn'nnn_ Cuts at: 2331 2331 Size: 6067 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 4315 6027 4315 Size: 1712 4355 SfcI C'TryA_G Cuts at: 826 1045 2405 3201 3211 3236 3415 4550 5231 Size: 219 1360 796 10 25 179 1135 681 Cuts at: 5231 5422 826 Size: 191 1471 Fragments arranged by size: 1471 1360 1135 796 681 219 191 179 25 10 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3065 4171 5072 5334 820 Size: 197 926 1122 1106 901 262 1553 Fragments arranged by size: 1553 1122 1106 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3103 3245 3425 1132 Size: 1971 142 180 3774 Fragments arranged by size: 3774 1971 180 142 SspI AAT'ATT Cuts at: 5 52 2542 3991 5 Size: 47 2490 1449 2081 Fragments arranged by size: 2490 2081 1449 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6067 StyI C'CwwG_G Cuts at: 513 1065 2748 3049 3249 3506 513 Size: 552 1683 301 200 257 3074 Fragments arranged by size: 3074 1683 552 301 257 200 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2230 2887 4094 4111 4264 4449 4894 Size: 1255 650 657 1207 17 153 185 445 Cuts at: 4894 325 Size: 1498 Fragments arranged by size: 1498 1255 1207 657 650 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 4313 6025 Size: 274 80 33 51 1443 54 2282 1712 Cuts at: 6025 96 Size: 138 Fragments arranged by size: 2282 1712 1443 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6027 6059 6027 Size: 32 6035 Tth111I GACn'n_nGTC Cuts at: 1316 2944 1316 Size: 1628 4439 XbaI T'CTAG_A Cuts at: 2260 3403 2260 Size: 1143 4924 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3292 3292 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6067 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 3084 4196 3084 Size: 1112 4955 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3875 276 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI