(Circular) (Six-base) MAPSORT of: pFN21AB6669.seq Check: 1416 from: 1 to: 5980 pFN21AB6669 5980 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3323 1937 Size: 1386 4594 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2481 2706 2792 3159 4043 5427 5695 1632 Size: 849 225 86 367 884 1384 268 1917 Fragments arranged by size: 1917 1384 884 849 367 268 225 86 AfeI AGC'GCT Cuts at: 2178 3072 2178 Size: 894 5086 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5783 AflIII A'CryG_T Cuts at: 1203 2417 4879 5856 1203 Size: 1214 2462 977 1327 Fragments arranged by size: 2462 1327 1214 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 2418 4709 2418 Size: 2291 3689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5980 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5980 AlwNI CAG_nnn'CTG Cuts at: 2870 3723 5295 2870 Size: 853 1572 3555 Fragments arranged by size: 3555 1572 853 ApaLI G'TGCA_C Cuts at: 3238 4037 5193 3238 Size: 799 1156 4025 Fragments arranged by size: 4025 1156 799 ApoI r'AATT_y Cuts at: 1493 2320 3082 3289 3515 3667 5776 1493 Size: 827 762 207 226 152 2109 1697 Fragments arranged by size: 2109 1697 827 762 226 207 152 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 2044 3305 1870 Size: 73 101 1261 4545 Fragments arranged by size: 4545 1261 101 73 BamHI G'GATC_C Cuts at: 3310 3677 3310 Size: 367 5613 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2218 3301 4756 618 Size: 325 217 627 431 1083 1455 1842 Fragments arranged by size: 1842 1455 1083 627 431 325 217 BanII G_rGCy'C Cuts at: 1391 2928 2984 3299 1391 Size: 1537 56 315 4072 Fragments arranged by size: 4072 1537 315 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5009 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3119 3153 4171 4205 1903 Size: 34 1182 34 1018 34 3678 Fragments arranged by size: 3678 1182 1018 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3872 5082 3872 Size: 1210 4770 BclI T'GATC_A Cuts at: 1548 1548 Size: 5980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2786 4591 136 Size: 107 122 71 631 605 1114 1805 1525 Fragments arranged by size: 1805 1525 1114 631 605 122 107 71 BglII A'GATC_T Cuts at: 2040 2607 5975 2040 Size: 567 3368 2045 Fragments arranged by size: 3368 2045 567 BlpI GC'TnA_GC Cuts at: 3397 3397 Size: 5980 Bme1580I G_kGCm'C Cuts at: 1790 3242 4041 5197 1790 Size: 1452 799 1156 2573 Fragments arranged by size: 2573 1452 1156 799 BmgBI CAC'GTC Cuts at: 1318 3237 1318 Size: 1919 4061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2463 3711 4669 Size: 416 53 83 399 24 1046 1248 958 Cuts at: 4669 442 Size: 1753 Fragments arranged by size: 1753 1248 1046 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5980 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2788 2788 Size: 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2760 4640 1239 Size: 1521 1880 2579 Fragments arranged by size: 2579 1880 1521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2750 4105 4970 5268 2750 Size: 1355 865 298 3462 Fragments arranged by size: 3462 1355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3196 4643 882 Size: 617 1697 1447 2219 Fragments arranged by size: 2219 1697 1447 617 BsaAI yAC'GTr Cuts at: 493 1204 2946 3771 493 Size: 711 1742 825 2702 Fragments arranged by size: 2702 1742 825 711 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3787 4169 4804 275 Size: 53 83 186 936 2254 382 635 1451 Fragments arranged by size: 2254 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4413 4811 5085 5232 5643 4413 Size: 398 274 147 411 4750 Fragments arranged by size: 4750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5755 Fragments arranged by size: 5755 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 1186 Size: 1069 4911 BseYI C'CCAG_C Cuts at: 5183 5679 5183 Size: 496 5484 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3087 3199 1163 Size: 573 1351 112 3944 Fragments arranged by size: 3944 1351 573 112 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3430 4191 5219 1540 Size: 275 186 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2906 2963 2984 3242 4041 4126 5197 2034 Size: 872 57 21 258 799 85 1071 2817 Fragments arranged by size: 2817 1071 872 799 258 85 57 21 BsmI GAATG_Cn' Cuts at: 3034 3491 3584 3034 Size: 457 93 5430 Fragments arranged by size: 5430 457 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2906 2928 2963 2984 3242 3299 Size: 399 244 872 22 35 21 258 57 Cuts at: 3299 4041 4126 5197 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 872 742 399 258 244 85 57 35 22 21 BspEI T'CCGG_A Cuts at: 5643 5643 Size: 5980 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3335 844 Size: 2491 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3867 1394 Size: 429 2044 3507 Fragments arranged by size: 3507 2044 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2563 4475 4649 65 Size: 1183 1315 1912 174 1396 Fragments arranged by size: 1912 1396 1315 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4624 4811 1700 Size: 110 2814 187 2869 Fragments arranged by size: 2869 2814 187 110 BsrGI T'GTAC_A Cuts at: 96 2110 96 Size: 2014 3966 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 4040 5052 4040 Size: 1012 4968 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5980 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5980 BstYI r'GATC_y Cuts at: 1972 2040 2607 3310 3677 4062 4079 5531 5617 Size: 68 567 703 367 385 17 1452 86 Cuts at: 5617 5629 5975 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 703 567 385 367 346 86 68 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4489 Fragments arranged by size: 4489 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5241 Fragments arranged by size: 5241 683 56 BtsI GCAGTG_nn' Cuts at: 2032 3211 3496 4290 4310 2032 Size: 1179 285 794 20 3702 Fragments arranged by size: 3702 1179 794 285 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5945 DraI TTT'AAA Cuts at: 2326 3284 2326 Size: 958 5022 DraIII CAC_nnn'GTG Cuts at: 2234 2234 Size: 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3427 4316 5945 Size: 54 1425 50 275 18 1597 889 1629 Cuts at: 5945 8 Size: 43 Fragments arranged by size: 1629 1597 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3427 1537 Size: 275 1615 4090 Fragments arranged by size: 4090 1615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2619 3910 1353 Size: 1266 1291 3423 Fragments arranged by size: 3423 1291 1266 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3007 4567 4941 5087 387 Size: 1064 138 1418 1560 374 146 1280 Fragments arranged by size: 1560 1418 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2982 2982 Size: 5980 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2481 2706 2760 2792 3159 4043 4640 Size: 393 849 225 54 32 367 884 597 Cuts at: 4640 5427 5695 1239 Size: 787 268 1524 Fragments arranged by size: 1524 884 849 787 597 393 367 268 225 54 32 EcoNI CCTnn'n_nnAGG Cuts at: 2919 2919 Size: 5980 EcoO109I rG'GnC_Cy Cuts at: 2487 2487 Size: 5980 EcoRI G'AATT_C Cuts at: 3082 3289 3667 3082 Size: 207 378 5395 Fragments arranged by size: 5395 378 207 FspI TGC'GCA Cuts at: 4486 4486 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 2180 3074 5127 1678 Size: 502 894 2053 2531 Fragments arranged by size: 2531 2053 894 502 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2404 2436 4621 4653 4695 Size: 32 135 32 832 32 2185 32 42 Cuts at: 4695 4727 1373 Size: 32 2626 Fragments arranged by size: 2626 2185 832 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3324 3570 669 Size: 988 281 1386 246 3079 Fragments arranged by size: 3079 1386 988 281 246 HindIII A'AGCT_T Cuts at: 748 3197 748 Size: 2449 3531 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2056 5094 5278 1305 Size: 751 3038 184 2007 Fragments arranged by size: 3038 2007 751 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4158 Fragments arranged by size: 4158 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2873 3938 4297 4456 518 Size: 648 1707 1065 359 159 2042 Fragments arranged by size: 2042 1707 1065 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2674 2884 3396 4073 5221 5466 1606 Size: 1068 210 512 677 1148 245 2120 Fragments arranged by size: 2120 1148 1068 677 512 245 210 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4489 Fragments arranged by size: 4489 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5980 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 2421 3338 4883 1132 Size: 97 1192 917 1545 2229 Fragments arranged by size: 2229 1545 1192 917 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5980 PciI A'CATG_T Cuts at: 2417 4879 2417 Size: 2462 3518 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5980 PfoI T'CCnGG_A Cuts at: 2760 2760 Size: 5980 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2487 2487 Size: 5980 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 2309 3550 2309 Size: 1241 4739 PstI C_TGCA'G Cuts at: 830 2484 3332 830 Size: 1654 848 3478 Fragments arranged by size: 3478 1654 848 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 PvuII CAG'CTG Cuts at: 2674 2674 Size: 5980 SacI G_AGCT'C Cuts at: 2984 2984 Size: 5980 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SfcI C'TryA_G Cuts at: 826 1045 2480 2491 3328 4463 5144 5335 826 Size: 219 1435 11 837 1135 681 191 1471 Fragments arranged by size: 1471 1435 1135 837 681 219 191 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 3307 1872 Size: 1435 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2729 4084 4985 5247 820 Size: 197 926 786 1355 901 262 1553 Fragments arranged by size: 1553 1355 926 901 786 262 197 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SspI AAT'ATT Cuts at: 5 52 2660 3904 5 Size: 47 2608 1244 2081 Fragments arranged by size: 2608 2081 1244 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5980 StyI C'CwwG_G Cuts at: 513 1065 2004 2985 3419 513 Size: 552 939 981 434 3074 Fragments arranged by size: 3074 981 939 552 434 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2860 2946 4007 4024 4177 4362 4807 Size: 1255 1280 86 1061 17 153 185 445 Cuts at: 4807 325 Size: 1498 Fragments arranged by size: 1498 1280 1255 1061 445 185 153 86 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2110 4226 5938 Size: 274 80 33 51 1443 133 2116 1712 Cuts at: 5938 96 Size: 138 Fragments arranged by size: 2116 1712 1443 274 138 133 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3232 3264 5940 5972 3232 Size: 32 2676 32 3240 Fragments arranged by size: 3240 2676 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5980 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5980 XmaI C'CCGG_G Cuts at: 1870 3305 1870 Size: 1435 4545 XmnI GAAnn'nnTTC Cuts at: 2805 4109 2805 Size: 1304 4676 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI