(Circular) (Six-base) MAPSORT of: pFN21AB6658.seq Check: 6036 from: 1 to: 6058 pFN21AB6658 6058 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2878 2878 Size: 6058 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2106 3027 3114 3868 Size: 53 83 186 936 570 921 87 754 Cuts at: 3868 278 Size: 2468 Fragments arranged by size: 2468 936 921 754 570 186 87 83 53 AccI GT'mk_AC Cuts at: 1937 3027 3401 1937 Size: 1090 374 4594 Fragments arranged by size: 4594 1090 374 Acc65I G'GTAC_C Cuts at: 3379 3379 Size: 6058 AclI AA'CG_TT Cuts at: 1563 4185 4558 1563 Size: 2622 373 3063 Fragments arranged by size: 3063 2622 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3093 4121 5505 5773 1632 Size: 1461 1028 1384 268 1917 Fragments arranged by size: 1917 1461 1384 1028 268 AfeI AGC'GCT Cuts at: 3078 3078 Size: 6058 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 4957 5934 1203 Size: 3754 977 1327 Fragments arranged by size: 3754 1327 977 AgeI A'CCGG_T Cuts at: 4889 4889 Size: 6058 AhdI GACnn_n'nnGTC Cuts at: 2062 3115 4787 2062 Size: 1053 1672 3333 Fragments arranged by size: 3333 1672 1053 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6058 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6058 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2222 2254 2222 Size: 32 6026 AlwNI CAG_nnn'CTG Cuts at: 2482 2698 3801 5373 2482 Size: 216 1103 1572 3167 Fragments arranged by size: 3167 1572 1103 216 ApaI G_GGCC'C Cuts at: 2036 2476 2036 Size: 440 5618 ApaLI G'TGCA_C Cuts at: 3251 4115 5271 3251 Size: 864 1156 4038 Fragments arranged by size: 4038 1156 864 ApoI r'AATT_y Cuts at: 1493 3367 3593 3745 5854 1493 Size: 1874 226 152 2109 1697 Fragments arranged by size: 2109 1874 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4612 160 Size: 872 3580 1606 Fragments arranged by size: 3580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2450 2670 3084 3287 3383 1870 Size: 73 70 437 220 414 203 96 4545 Fragments arranged by size: 4545 437 414 220 203 96 73 70 BamHI G'GATC_C Cuts at: 3388 3755 3388 Size: 367 5691 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 3160 3379 4834 618 Size: 325 217 627 516 857 219 1455 1842 Fragments arranged by size: 1842 1455 857 627 516 325 219 217 BanII G_rGCy'C Cuts at: 1391 2036 2476 2535 2631 2802 3377 1391 Size: 645 440 59 96 171 575 4072 Fragments arranged by size: 4072 645 575 440 171 96 59 BbeI G_GCGC'C Cuts at: 2307 2307 Size: 6058 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2098 2611 928 Size: 971 199 513 4375 Fragments arranged by size: 4375 971 513 199 BbvCI CC'TCA_GC Cuts at: 2441 2895 2441 Size: 454 5604 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4249 4283 1903 Size: 34 2312 34 3678 Fragments arranged by size: 3678 2312 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3950 5160 3950 Size: 1210 4848 BclI T'GATC_A Cuts at: 1548 2313 1548 Size: 765 5293 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4669 136 Size: 107 122 71 631 605 2997 1525 Fragments arranged by size: 2997 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2388 6053 2388 Size: 3665 2393 BlpI GC'TnA_GC Cuts at: 2084 3475 2084 Size: 1391 4667 Bme1580I G_kGCm'C Cuts at: 1790 2036 2193 2476 3165 3242 3255 4119 5275 Size: 246 157 283 689 77 13 864 1156 Cuts at: 5275 1790 Size: 2573 Fragments arranged by size: 2573 1156 864 689 283 246 157 77 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2563 3789 4747 Size: 416 53 83 399 24 1146 1226 958 Cuts at: 4747 442 Size: 1753 Fragments arranged by size: 1753 1226 1146 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6058 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2696 3343 2696 Size: 647 5411 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2843 2933 3254 3313 4718 1239 Size: 1604 90 321 59 1405 2579 Fragments arranged by size: 2579 1604 1405 321 90 59 Bpu10I CC'TnA_GC Cuts at: 2441 2529 2895 2441 Size: 88 366 5604 Fragments arranged by size: 5604 366 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4183 5048 5346 4183 Size: 865 298 4895 Fragments arranged by size: 4895 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3289 4721 882 Size: 617 1790 1432 2219 Fragments arranged by size: 2219 1790 1432 617 BsaAI yAC'GTr Cuts at: 493 1204 2113 3849 493 Size: 711 909 1736 2702 Fragments arranged by size: 2702 1736 909 711 BsaBI GATnn'nnATC Cuts at: 6052 6052 Size: 6058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 2304 3024 3111 Size: 53 83 186 936 570 201 720 87 Cuts at: 3111 3207 3865 4247 4882 275 Size: 96 658 382 635 1451 Fragments arranged by size: 1451 936 720 658 635 570 382 201 186 96 87 83 53 BsaWI w'CCGG_w Cuts at: 4491 4889 5163 5310 5721 4491 Size: 398 274 147 411 4828 Fragments arranged by size: 4828 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2117 2120 2147 2150 1178 Size: 30 165 30 714 3 27 3 5086 Fragments arranged by size: 5086 714 165 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2141 2585 1186 Size: 907 48 444 4659 Fragments arranged by size: 4659 907 444 48 BseYI C'CCAG_C Cuts at: 2593 3245 5261 5757 2593 Size: 652 2016 496 2894 Fragments arranged by size: 2894 2016 652 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2399 2669 3298 1163 Size: 573 663 270 629 3923 Fragments arranged by size: 3923 663 629 573 270 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3357 3508 4269 5297 1540 Size: 275 186 1356 151 761 1028 2301 Fragments arranged by size: 2301 1356 1028 761 275 186 151 BsiHKAI G_wGCw'C Cuts at: 2802 3255 4119 4204 5275 2802 Size: 453 864 85 1071 3585 Fragments arranged by size: 3585 1071 864 453 85 BsmI GAATG_Cn' Cuts at: 3569 3662 3569 Size: 93 5965 BsmBI CGTCTCn'nnnn_ Cuts at: 2210 2210 Size: 6058 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2193 2476 2535 2631 2802 3165 Size: 399 246 157 283 59 96 171 363 Cuts at: 3165 3242 3255 3377 4119 4204 5275 1391 Size: 77 13 122 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 363 283 246 171 157 122 96 85 77 59 13 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 3947 5677 3947 Size: 1730 4328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2878 3413 844 Size: 2034 535 3489 Fragments arranged by size: 3489 2034 535 BsrBI CCG'CTC Cuts at: 1394 1823 2259 3945 1394 Size: 429 436 1686 3507 Fragments arranged by size: 3507 1686 436 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4553 4727 65 Size: 1183 3305 174 1396 Fragments arranged by size: 3305 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2409 3209 4702 4889 1700 Size: 110 599 800 1493 187 2869 Fragments arranged by size: 2869 1493 800 599 187 110 BsrGI T'GTAC_A Cuts at: 96 2784 96 Size: 2688 3370 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6058 BssSI C'ACGA_G Cuts at: 2522 4118 5130 2522 Size: 1596 1012 3450 Fragments arranged by size: 3450 1596 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2374 2698 3225 794 Size: 1580 324 527 3627 Fragments arranged by size: 3627 1580 527 324 BstBI TT'CG_AA Cuts at: 4862 4862 Size: 6058 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6058 BstXI CCAn_nnnn'nTGG Cuts at: 2044 2152 2044 Size: 108 5950 BstYI r'GATC_y Cuts at: 1972 2388 2709 3388 3755 4140 4157 5609 5695 Size: 416 321 679 367 385 17 1452 86 Cuts at: 5695 5707 6053 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 679 416 385 367 346 321 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3197 3197 Size: 6058 BtgI C'CryG_G Cuts at: 513 1065 2004 2172 2234 2298 2488 2760 2769 Size: 552 939 168 62 64 190 272 9 Cuts at: 2769 513 Size: 3802 Fragments arranged by size: 3802 939 552 272 190 168 64 62 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2863 487 Size: 683 56 1637 3682 Fragments arranged by size: 3682 1637 683 56 BtsI GCAGTG_nn' Cuts at: 2138 3574 4368 4388 2138 Size: 1436 794 20 3808 Fragments arranged by size: 3808 1436 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6023 DraI TTT'AAA Cuts at: 3362 3362 Size: 6058 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2224 5065 809 Size: 507 908 2841 1802 Fragments arranged by size: 2841 1802 908 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2205 2502 Size: 54 1425 50 275 18 285 90 297 Cuts at: 2502 2652 2805 2943 3505 4394 6023 8 Size: 150 153 138 562 889 1629 43 Fragments arranged by size: 1629 1425 889 562 297 285 275 153 150 138 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3505 1537 Size: 275 1693 4090 Fragments arranged by size: 4090 1693 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3988 1353 Size: 2635 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4645 5019 5165 387 Size: 1064 138 3056 374 146 1280 Fragments arranged by size: 3056 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2800 2800 Size: 6058 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2843 2933 3093 3254 3313 4121 4718 Size: 393 1211 90 160 161 59 808 597 Cuts at: 4718 5505 5773 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1211 808 787 597 393 268 161 160 90 59 EcoNI CCTnn'n_nnAGG Cuts at: 2423 3196 2423 Size: 773 5285 EcoO109I rG'GnC_Cy Cuts at: 2033 2472 2776 2938 3283 3310 2033 Size: 439 304 162 345 27 4781 Fragments arranged by size: 4781 439 345 304 162 27 EcoRI G'AATT_C Cuts at: 3367 3745 3367 Size: 378 5680 FspI TGC'GCA Cuts at: 4564 4564 Size: 6058 HaeII r_GCGC'y Cuts at: 1678 2307 2753 3080 5205 1678 Size: 629 446 327 2125 2531 Fragments arranged by size: 2531 2125 629 446 327 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2417 2449 3101 Size: 32 135 32 476 32 337 32 652 Cuts at: 3101 3133 4699 4731 4773 4805 1373 Size: 32 1566 32 42 32 2626 Fragments arranged by size: 2626 1566 652 476 337 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3402 3648 669 Size: 988 281 1464 246 3079 Fragments arranged by size: 3079 1464 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6058 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KasI G'GCGC_C Cuts at: 2303 2303 Size: 6058 KpnI G_GTAC'C Cuts at: 3383 3383 Size: 6058 MfeI C'AATT_G Cuts at: 3657 3657 Size: 6058 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 2685 3132 3204 5172 5356 1305 Size: 903 477 447 72 1968 184 2007 Fragments arranged by size: 2007 1968 903 477 447 184 72 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 2654 2807 10 Size: 54 1425 343 285 537 153 3261 Fragments arranged by size: 3261 1425 537 343 285 153 54 MslI CAynn'nnrTG Cuts at: 518 1166 2547 2660 3274 4016 4375 4534 518 Size: 648 1381 113 614 742 359 159 2042 Fragments arranged by size: 2042 1381 742 648 614 359 159 113 MspA1I CmG'CkG Cuts at: 1606 3474 4151 5299 5544 1606 Size: 1868 677 1148 245 2120 Fragments arranged by size: 2120 1868 1148 677 245 NaeI GCC'GGC Cuts at: 2411 3211 2411 Size: 800 5258 NarI GG'CG_CC Cuts at: 2304 2304 Size: 6058 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4567 Fragments arranged by size: 4567 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6058 NgoMIV G'CCGG_C Cuts at: 2409 3209 2409 Size: 800 5258 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6058 NotI GC'GGCC_GC Cuts at: 3505 3505 Size: 6058 NspI r_CATG'y Cuts at: 1132 1229 2905 3178 3223 3273 3416 4961 1132 Size: 97 1676 273 45 50 143 1545 2229 Fragments arranged by size: 2229 1676 1545 273 143 97 50 45 PasI CC'CwG_GG Cuts at: 1348 2179 1348 Size: 831 5227 PciI A'CATG_T Cuts at: 4957 4957 Size: 6058 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6058 PfoI T'CCnGG_A Cuts at: 2066 2066 Size: 6058 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PmlI CAC'GTG Cuts at: 2113 2113 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4127 4159 5640 5672 4127 Size: 32 1481 32 4513 Fragments arranged by size: 4513 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2776 2938 3283 2776 Size: 162 345 5551 Fragments arranged by size: 5551 345 162 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6058 PsiI TTA'TAA Cuts at: 3628 3628 Size: 6058 PspOMI G'GGCC_C Cuts at: 2032 2472 2032 Size: 440 5618 PstI C_TGCA'G Cuts at: 830 2283 2588 2703 3410 830 Size: 1453 305 115 707 3478 Fragments arranged by size: 3478 1453 707 305 115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 SacI G_AGCT'C Cuts at: 2802 2802 Size: 6058 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SanDI GG'GwC_CC Cuts at: 2776 3283 2776 Size: 507 5551 SbfI CC_TGCA'GG Cuts at: 3410 3410 Size: 6058 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SfcI C'TryA_G Cuts at: 826 1045 2279 2584 2699 3406 4541 5222 5413 Size: 219 1234 305 115 707 1135 681 191 Cuts at: 5413 826 Size: 1471 Fragments arranged by size: 1471 1234 1135 707 681 305 219 191 115 SfoI GGC'GCC Cuts at: 2305 2305 Size: 6058 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6058 SmaI CCC'GGG Cuts at: 1872 2015 3385 1872 Size: 143 1370 4545 Fragments arranged by size: 4545 1370 143 SmlI C'TyrA_G Cuts at: 820 1017 1943 2670 4162 5063 5325 820 Size: 197 926 727 1492 901 262 1553 Fragments arranged by size: 1553 1492 926 901 727 262 197 SnaBI TAC'GTA Cuts at: 493 3849 493 Size: 3356 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 3223 3416 1132 Size: 2091 193 3774 Fragments arranged by size: 3774 2091 193 SspI AAT'ATT Cuts at: 5 52 3982 5 Size: 47 3930 2081 Fragments arranged by size: 3930 2081 47 StuI AGG'CCT Cuts at: 1517 3191 1517 Size: 1674 4384 StyI C'CwwG_G Cuts at: 513 1065 2004 2808 3497 513 Size: 552 939 804 689 3074 Fragments arranged by size: 3074 939 804 689 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4085 4102 4255 4440 4885 325 Size: 1255 2505 17 153 185 445 1498 Fragments arranged by size: 2505 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2784 4304 6016 Size: 274 80 33 51 1443 807 1520 1712 Cuts at: 6016 96 Size: 138 Fragments arranged by size: 1712 1520 1443 807 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2117 2120 2149 2152 6018 6050 2117 Size: 3 29 3 3866 32 2125 Fragments arranged by size: 3866 2125 32 29 3 3 Tth111I GACn'n_nGTC Cuts at: 1316 2345 2642 1316 Size: 1029 297 4732 Fragments arranged by size: 4732 1029 297 XbaI T'CTAG_A Cuts at: 3394 3394 Size: 6058 XhoI C'TCGA_G Cuts at: 1943 2670 1943 Size: 727 5331 XmaI C'CCGG_G Cuts at: 1870 2013 3383 1870 Size: 143 1370 4545 Fragments arranged by size: 4545 1370 143 XmnI GAAnn'nnTTC Cuts at: 4187 4187 Size: 6058 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2104 3025 3112 3866 Size: 53 83 186 936 570 921 87 754 Cuts at: 3866 276 Size: 2468 Fragments arranged by size: 2468 936 921 754 570 186 87 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI