Restriction map for
pFN21AB6647
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MaeII
MamI MluI MroI MseI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI StyI SwaI Tru9I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 349 GTCTAC GT^[AC][GT]AC
AfaI 1199 GTAC GT^AC
AflIII 427 ACATGT A^C[GA][CT]GT
AspHI 65 GTGCTC G[AT]GC[AT]^C
AspI 324 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 590 CCCGGG C^[CT]CG[GA]G
AviII 659 TGCGCA TGC^GCA
BalI 905 TGGCCA TGG^CCA
BbvI 4 GCTGC GCAGC(8/12)
BfaI 1021 CTAG C^TAG
BsaI 322 GAGACC GGTCTC(1/5)
BsaWI 290 TCCGGT [AT]^CCGG[AT]
BsgI 1154 GTGCAG GTGCAG(16/14)
BsiHKAI 65 GTGCTC G[AT]GC[AT]^C
BsoBI 590 CCCGGG C^[CT]CG[GA]G
BspLU11I 427 ACATGT A^CATGT
BsrDI 301 GCAATG GCAATG(2/0)
BssSI 227 CTCGTG CACGAG(-5/-1)
BstEII 579 GGTGACC G^GT[ACGT]ACC
BstPI 579 GGTGACC G^GT[ACGT]ACC
CfoI 660 GCGC GCG^C
DrdI 426 GACATGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 921 CCACGG C^C[GA][CT]GG
EaeI 905 TGGCCA [CT]^GGCC[GA]
EcoO65I 579 GGTGACC G^GT[ACGT]ACC
FspI 659 TGCGCA TGC^GCA
HhaI 660 GCGC GCG^C
HinP1I 660 GCGC G^CGC
HindIII 176 AAGCTT A^AGCTT
HphI 579 GGTGA GGTGA(8/7)
MaeI 1021 CTAG C^TAG
MfeI 715 CAATTG C^AATTG
MluNI 905 TGGCCA TGG^CCA
MscI 905 TGGCCA TGG^CCA
MslI 472 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 715 CAATTG C^AATTG
NlaIII 428 CATG CATG^
NspI 427 ACATGT [GA]CATG^[CT]
PflFI 324 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 185 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 897 CAGCTG CAG^CTG
RsaI 1199 GTAC GT^AC
SapI 552 GCTCTTC GCTCTTC(1/4)
SbfI 451 CCTGCAGG CCTGCA^GG
ScaI 1198 AGTACT AGT^ACT
SmaI 590 CCCGGG CCC^GGG
Sse8387I 451 CCTGCAGG CCTGCA^GG
TaqI 962 TCGA T^CGA
TseI 4 GCTGC G^C[AT]GC
Tsp45I 580 GTGAC ^GT[GC]AC
Tth111I 324 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 962 TCGA T^CGA
XmaCI 590 CCCGGG C^CCGGG
XmaI 590 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwNI 139 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
989 CAGGCTCTG
AocI 877 CCTCAGG CC^T[ACGT]AGG
1180 CCTGAGG
ApaI 119 GGGCCC GGGCC^C
925 GGGCCC
BamHI 269 GGATCC G^GATCC
1037 GGATCC
BanI 293 GGTGCC G^G[CT][GA]CC
492 GGTGCC
BpmI 703 CTGGAG CTGGAG(16/14)
1202 CTGGAG
BstXI 354 CCAGATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
413 CCAATAAGGTGG
Bsu36I 877 CCTCAGG CC^T[ACGT]AGG
1180 CCTGAGG
Eco81I 877 CCTCAGG CC^T[ACGT]AGG
1180 CCTGAGG
MaeIII 580 GTGAC ^GT[ACGT]AC
763 GTAAC
MspA1I 139 CAGCGG C[AC]G^C[GT]G
897 CAGCTG
PleI 459 GACTC GAGTC(4/5)
341 GAGTC
SfaNI 950 GCATC GCATC(5/9)
956 GCATC
StuI 636 AGGCCT AGG^CCT
1047 AGGCCT
Tsp509I 375 AATT ^AATT
716 AATT
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIV PflMI PstI Sau3AI Sau96I
ScrFI SfcI TfiI TspRI Van91I
XhoII