(Circular) (Six-base) MAPSORT of: pFN21AB6647.seq Check: 5038 from: 1 to: 5917 pFN21AB6647 5917 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2355 3260 1937 Size: 418 905 4594 Fragments arranged by size: 4594 905 418 Acc65I G'GTAC_C Cuts at: 3238 3238 Size: 5917 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2132 2617 3980 5364 5632 1632 Size: 500 485 1363 1384 268 1917 Fragments arranged by size: 1917 1384 1363 500 485 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 2432 4816 5793 1203 Size: 1229 2384 977 1327 Fragments arranged by size: 2384 1327 1229 977 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5917 AlwNI CAG_nnn'CTG Cuts at: 2149 2999 3660 5232 2149 Size: 850 661 1572 2834 Fragments arranged by size: 2834 1572 850 661 ApaI G_GGCC'C Cuts at: 2128 2934 2128 Size: 806 5111 ApaLI G'TGCA_C Cuts at: 3974 5130 3974 Size: 1156 4761 ApoI r'AATT_y Cuts at: 1493 3226 3452 3604 5713 1493 Size: 1733 226 152 2109 1697 Fragments arranged by size: 2109 1733 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4471 160 Size: 872 3439 1606 Fragments arranged by size: 3439 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 2595 3242 1870 Size: 73 652 647 4545 Fragments arranged by size: 4545 652 647 73 BamHI G'GATC_C Cuts at: 2274 3042 3247 3614 2274 Size: 768 205 367 4577 Fragments arranged by size: 4577 768 367 205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2298 2497 3238 4693 618 Size: 325 217 627 511 199 741 1455 1842 Fragments arranged by size: 1842 1455 741 627 511 325 217 199 BanII G_rGCy'C Cuts at: 1391 2128 2559 2934 3236 1391 Size: 737 431 375 302 4072 Fragments arranged by size: 4072 737 431 375 302 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4946 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4108 4142 1903 Size: 34 2171 34 3678 Fragments arranged by size: 3678 2171 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3809 5019 3809 Size: 1210 4707 BclI T'GATC_A Cuts at: 1548 1548 Size: 5917 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4528 136 Size: 107 122 71 631 605 2856 1525 Fragments arranged by size: 2856 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5912 5912 Size: 5917 BlpI GC'TnA_GC Cuts at: 3334 3334 Size: 5917 Bme1580I G_kGCm'C Cuts at: 1790 2128 2835 2934 3978 5134 1790 Size: 338 707 99 1044 1156 2573 Fragments arranged by size: 2573 1156 1044 707 338 99 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5917 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3648 4606 442 Size: 416 53 83 399 24 2231 958 1753 Fragments arranged by size: 2231 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2729 3228 4577 1239 Size: 1490 499 1349 2579 Fragments arranged by size: 2579 1490 1349 499 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4042 4907 5205 4042 Size: 865 298 4754 Fragments arranged by size: 4754 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2321 4580 882 Size: 617 822 2259 2219 Fragments arranged by size: 2259 2219 822 617 BsaAI yAC'GTr Cuts at: 493 1204 3708 493 Size: 711 2504 2702 Fragments arranged by size: 2702 2504 711 BsaBI GATnn'nnATC Cuts at: 5911 5911 Size: 5917 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3724 4106 4741 275 Size: 53 83 186 936 2191 382 635 1451 Fragments arranged by size: 2191 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2295 4350 4748 5022 5169 5580 2295 Size: 2055 398 274 147 411 2632 Fragments arranged by size: 2632 2055 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2019 2049 2318 2348 2505 Size: 30 165 30 616 30 269 30 157 Cuts at: 2505 2535 3201 3231 1178 Size: 30 666 30 3864 Fragments arranged by size: 3864 666 616 269 165 157 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2223 2616 2726 2793 2993 3126 3131 3164 Size: 1037 393 110 67 200 133 5 33 Cuts at: 3164 3167 1186 Size: 3 3936 Fragments arranged by size: 3936 1037 393 200 133 110 67 33 5 3 BseYI C'CCAG_C Cuts at: 2142 2590 2793 2849 3016 5120 5616 2142 Size: 448 203 56 167 2104 496 2443 Fragments arranged by size: 2443 2104 496 448 203 167 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3180 1163 Size: 573 1444 3900 Fragments arranged by size: 3900 1444 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3367 4128 5156 1540 Size: 275 186 1366 761 1028 2301 Fragments arranged by size: 2301 1366 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2074 3978 4063 5134 2074 Size: 1904 85 1071 2857 Fragments arranged by size: 2857 1904 1071 85 BsmI GAATG_Cn' Cuts at: 3428 3521 3428 Size: 93 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2128 2559 2835 2934 3236 3978 Size: 399 284 54 431 276 99 302 742 Cuts at: 3978 4063 5134 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 431 399 302 284 276 99 85 54 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 3806 5536 3806 Size: 1730 4187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3272 844 Size: 2428 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3804 1394 Size: 429 1981 3507 Fragments arranged by size: 3507 1981 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2313 4412 4586 65 Size: 1183 1065 2099 174 1396 Fragments arranged by size: 2099 1396 1183 1065 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4561 4748 1700 Size: 110 2751 187 2869 Fragments arranged by size: 2869 2751 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 2232 3977 4989 2232 Size: 1745 1012 3160 Fragments arranged by size: 3160 1745 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5917 BstBI TT'CG_AA Cuts at: 4721 4721 Size: 5917 BstEII G'GTnAC_C Cuts at: 1184 2584 1184 Size: 1400 4517 BstXI CCAn_nnnn'nTGG Cuts at: 2366 2425 2366 Size: 59 5858 BstYI r'GATC_y Cuts at: 1972 2274 2605 3042 3247 3614 3999 4016 5468 Size: 302 331 437 205 367 385 17 1452 Cuts at: 5468 5554 5566 5912 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 437 385 367 346 331 302 205 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2883 3186 2883 Size: 303 5614 BtgI C'CryG_G Cuts at: 513 1065 2004 2926 513 Size: 552 939 922 3504 Fragments arranged by size: 3504 939 922 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5178 Fragments arranged by size: 5178 683 56 BtsI GCAGTG_nn' Cuts at: 2564 3433 4227 4247 2564 Size: 869 794 20 4234 Fragments arranged by size: 4234 869 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 3221 3221 Size: 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2437 4924 809 Size: 507 1121 2487 1802 Fragments arranged by size: 2487 1802 1121 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2910 3364 4253 Size: 54 1425 50 275 18 1080 454 889 Cuts at: 4253 5882 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1080 889 454 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3364 1537 Size: 275 1552 4090 Fragments arranged by size: 4090 1552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2261 2564 3141 3847 1353 Size: 908 303 577 706 3423 Fragments arranged by size: 3423 908 706 577 303 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4504 4878 5024 387 Size: 1064 138 2915 374 146 1280 Fragments arranged by size: 2915 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2617 2729 3228 3980 4577 5364 Size: 393 500 485 112 499 752 597 787 Cuts at: 5364 5632 1239 Size: 268 1524 Fragments arranged by size: 1524 787 752 597 500 499 485 393 268 112 EcoNI CCTnn'n_nnAGG Cuts at: 2550 2874 3189 2550 Size: 324 315 5278 Fragments arranged by size: 5278 324 315 EcoO109I rG'GnC_Cy Cuts at: 2124 2125 2191 2213 2879 2931 3127 3212 2124 Size: 1 66 22 666 52 196 85 4829 Fragments arranged by size: 4829 666 196 85 66 52 22 1 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 FspI TGC'GCA Cuts at: 2666 4423 2666 Size: 1757 4160 HaeII r_GCGC'y Cuts at: 1678 5064 1678 Size: 3386 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2318 2337 2350 2369 2378 Size: 32 135 32 746 19 13 19 9 Cuts at: 2378 2410 2866 2898 4558 4590 4632 4664 1373 Size: 32 456 32 1660 32 42 32 2626 Fragments arranged by size: 2626 1660 746 456 135 42 32 32 32 32 32 32 19 19 13 9 HincII GTy'rAC Cuts at: 669 1657 1938 3261 3507 669 Size: 988 281 1323 246 3079 Fragments arranged by size: 3079 1323 988 281 246 HindIII A'AGCT_T Cuts at: 748 2181 748 Size: 1433 4484 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KpnI G_GTAC'C Cuts at: 3242 3242 Size: 5917 MfeI C'AATT_G Cuts at: 2720 3516 2720 Size: 796 5121 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2005 2361 2553 3156 5031 5215 1305 Size: 700 356 192 603 1875 184 2007 Fragments arranged by size: 2007 1875 700 603 356 192 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2912 10 Size: 54 1425 343 1080 3015 Fragments arranged by size: 3015 1425 1080 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2481 3875 4234 4393 518 Size: 648 1315 1394 359 159 2042 Fragments arranged by size: 2042 1394 1315 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2146 2904 3333 4010 5158 5403 1606 Size: 540 758 429 677 1148 245 2120 Fragments arranged by size: 2120 1148 758 677 540 429 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4426 Fragments arranged by size: 4426 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 2436 3275 4820 1132 Size: 97 1207 839 1545 2229 Fragments arranged by size: 2229 1545 1207 839 97 PasI CC'CwG_GG Cuts at: 1348 2203 2217 3037 1348 Size: 855 14 820 4228 Fragments arranged by size: 4228 855 820 14 PciI A'CATG_T Cuts at: 2432 4816 2432 Size: 2384 3533 PflMI CCAn_nnn'nTGG Cuts at: 1108 2149 2725 2741 1108 Size: 1041 576 16 4284 Fragments arranged by size: 4284 1041 576 16 PfoI T'CCnGG_A Cuts at: 2277 2277 Size: 5917 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2191 2191 Size: 5917 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5917 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PspOMI G'GGCC_C Cuts at: 2124 2930 2124 Size: 806 5111 PstI C_TGCA'G Cuts at: 830 2461 2571 2995 3269 830 Size: 1631 110 424 274 3478 Fragments arranged by size: 3478 1631 424 274 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 PvuII CAG'CTG Cuts at: 2904 2904 Size: 5917 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SapI GCTCTTCn'nnn_ Cuts at: 2564 2564 Size: 5917 SbfI CC_TGCA'GG Cuts at: 2461 3269 2461 Size: 808 5109 ScaI AGT'ACT Cuts at: 3205 4165 5877 3205 Size: 960 1712 3245 Fragments arranged by size: 3245 1712 960 SfcI C'TryA_G Cuts at: 826 1045 2457 2567 2626 2899 2991 3265 4400 Size: 219 1412 110 59 273 92 274 1135 Cuts at: 4400 5081 5272 826 Size: 681 191 1471 Fragments arranged by size: 1471 1412 1135 681 274 273 219 191 110 92 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 2597 3244 1872 Size: 725 647 4545 Fragments arranged by size: 4545 725 647 SmlI C'TyrA_G Cuts at: 820 1017 1943 4021 4922 5184 820 Size: 197 926 2078 901 262 1553 Fragments arranged by size: 2078 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 2643 3054 1517 Size: 1126 411 4380 Fragments arranged by size: 4380 1126 411 StyI C'CwwG_G Cuts at: 513 1065 2004 3356 513 Size: 552 939 1352 3074 Fragments arranged by size: 3074 1352 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2023 2930 3944 3961 4114 4299 4744 Size: 1255 443 907 1014 17 153 185 445 Cuts at: 4744 325 Size: 1498 Fragments arranged by size: 1498 1255 1014 907 445 443 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3203 4163 5875 Size: 274 80 33 51 1443 1226 960 1712 Cuts at: 5875 96 Size: 138 Fragments arranged by size: 1712 1443 1226 960 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2019 2051 2505 2537 5877 5909 2019 Size: 32 454 32 3340 32 2027 Fragments arranged by size: 3340 2027 454 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2332 1316 Size: 1016 4901 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5917 XmaI C'CCGG_G Cuts at: 1870 2595 3242 1870 Size: 725 647 4545 Fragments arranged by size: 4545 725 647 XmnI GAAnn'nnTTC Cuts at: 4046 4046 Size: 5917 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI