(Circular) (Six-base) MAPSORT of: pFN21AB6645.seq Check: 1061 from: 1 to: 5962 pFN21AB6645 5962 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2246 2246 Size: 5962 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2165 3010 4025 5409 5677 1632 Size: 533 845 1015 1384 268 1917 Fragments arranged by size: 1917 1384 1015 845 533 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 2130 4861 5838 1203 Size: 927 2731 977 1327 Fragments arranged by size: 2731 1327 977 927 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2852 3173 1184 Size: 1668 321 3973 Fragments arranged by size: 3973 1668 321 AlwNI CAG_nnn'CTG Cuts at: 3705 5277 3705 Size: 1572 4390 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 3271 3497 3649 5758 1493 Size: 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 3287 1870 Size: 73 1344 4545 Fragments arranged by size: 4545 1344 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2522 2638 2731 3283 4738 Size: 325 217 627 735 116 93 552 1455 Cuts at: 4738 618 Size: 1842 Fragments arranged by size: 1842 1455 735 627 552 325 217 116 93 BanII G_rGCy'C Cuts at: 1391 2226 3281 1391 Size: 835 1055 4072 Fragments arranged by size: 4072 1055 835 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2539 2603 2984 3002 928 Size: 971 640 64 381 18 3888 Fragments arranged by size: 3888 971 640 381 64 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3109 3143 4153 4187 1903 Size: 34 1172 34 1010 34 3678 Fragments arranged by size: 3678 1172 1010 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3123 5957 3123 Size: 2834 3128 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 2197 2850 3152 4023 5179 1790 Size: 407 653 302 871 1156 2573 Fragments arranged by size: 2573 1156 871 653 407 302 BmgBI CAC'GTC Cuts at: 1318 2908 1318 Size: 1590 4372 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3199 3693 4651 Size: 416 53 83 399 24 1782 494 958 Cuts at: 4651 442 Size: 1753 Fragments arranged by size: 1782 1753 958 494 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2900 4622 1239 Size: 1661 1722 2579 Fragments arranged by size: 2579 1722 1661 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2879 2951 3203 4087 4952 5250 2879 Size: 72 252 884 865 298 3591 Fragments arranged by size: 3591 884 865 298 252 72 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2573 2772 4625 882 Size: 617 1074 199 1853 2219 Fragments arranged by size: 2219 1853 1074 617 199 BsaAI yAC'GTr Cuts at: 493 1204 2932 3753 493 Size: 711 1728 821 2702 Fragments arranged by size: 2702 1728 821 711 BsaBI GATnn'nnATC Cuts at: 2678 5956 2678 Size: 3278 2684 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 4151 4786 275 Size: 53 83 186 936 2236 382 635 1451 Fragments arranged by size: 2236 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2117 2147 2684 2714 1178 Size: 30 165 30 714 30 537 30 4426 Fragments arranged by size: 4426 714 537 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 2417 2783 2816 3242 3245 1186 Size: 1228 3 366 33 426 3 3903 Fragments arranged by size: 3903 1228 426 366 33 3 3 BseYI C'CCAG_C Cuts at: 2111 2219 2815 3097 5165 5661 2111 Size: 108 596 282 2068 496 2412 Fragments arranged by size: 2412 2068 596 496 282 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2812 1163 Size: 573 1076 4313 Fragments arranged by size: 4313 1076 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2856 3412 4173 5201 1540 Size: 275 186 855 556 761 1028 2301 Fragments arranged by size: 2301 1028 855 761 556 275 186 BsiHKAI G_wGCw'C Cuts at: 4023 4108 5179 4023 Size: 85 1071 4806 Fragments arranged by size: 4806 1071 85 BsiWI C'GTAC_G Cuts at: 2928 2928 Size: 5962 BsmI GAATG_Cn' Cuts at: 2320 3473 3566 2320 Size: 1153 93 4716 Fragments arranged by size: 4716 1153 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2197 2226 2850 3152 3281 4023 4108 Size: 399 407 29 624 302 129 742 85 Cuts at: 4108 5179 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 624 407 399 302 129 85 29 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 3851 5581 3851 Size: 1730 4232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2246 2790 3317 844 Size: 1402 544 527 3489 Fragments arranged by size: 3489 1402 544 527 BsrBI CCG'CTC Cuts at: 1394 1823 3849 1394 Size: 429 2026 3507 Fragments arranged by size: 3507 2026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2205 3145 4457 4631 65 Size: 1183 957 940 1312 174 1396 Fragments arranged by size: 1396 1312 1183 957 940 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4606 4793 1700 Size: 110 2796 187 2869 Fragments arranged by size: 2869 2796 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 2262 794 Size: 1468 4494 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 3073 1184 Size: 1889 4073 BstXI CCAn_nnnn'nTGG Cuts at: 2212 2654 2212 Size: 442 5520 BstYI r'GATC_y Cuts at: 1972 2937 3036 3123 3292 3659 4044 4061 5513 Size: 965 99 87 169 367 385 17 1452 Cuts at: 5513 5599 5611 5957 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 965 385 367 346 169 99 87 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3225 3225 Size: 5962 BtgI C'CryG_G Cuts at: 513 1065 2082 2850 3144 513 Size: 552 1017 768 294 3331 Fragments arranged by size: 3331 1017 768 552 294 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3184 487 Size: 683 56 1958 3265 Fragments arranged by size: 3265 1958 683 56 BtsI GCAGTG_nn' Cuts at: 3478 4272 4292 3478 Size: 794 20 5148 Fragments arranged by size: 5148 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2537 4969 809 Size: 507 1221 2432 1802 Fragments arranged by size: 2432 1802 1221 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2676 2755 2853 Size: 54 1425 50 275 18 846 79 98 Cuts at: 2853 3180 3409 4298 5927 8 Size: 327 229 889 1629 43 Fragments arranged by size: 1629 1425 889 846 327 275 229 98 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2853 3409 1537 Size: 275 1041 556 4090 Fragments arranged by size: 4090 1041 556 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3892 1353 Size: 2539 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2900 3010 4025 4622 5409 5677 Size: 393 533 735 110 1015 597 787 268 Cuts at: 5677 1239 Size: 1524 Fragments arranged by size: 1524 1015 787 735 597 533 393 268 110 EcoNI CCTnn'n_nnAGG Cuts at: 2378 2378 Size: 5962 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2197 2197 Size: 5962 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 5109 1678 Size: 3431 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2684 2716 4603 4635 4677 Size: 32 135 32 1112 32 1887 32 42 Cuts at: 4677 4709 1373 Size: 32 2626 Fragments arranged by size: 2626 1887 1112 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3195 5076 5260 1305 Size: 1890 1881 184 2007 Fragments arranged by size: 2007 1890 1881 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2678 3182 10 Size: 54 1425 343 846 504 2790 Fragments arranged by size: 2790 1425 846 504 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 2324 3920 4279 4438 518 Size: 648 1044 114 1596 359 159 2042 Fragments arranged by size: 2042 1596 1044 648 359 159 114 MspA1I CmG'CkG Cuts at: 1606 3378 4055 5203 5448 1606 Size: 1772 677 1148 245 2120 Fragments arranged by size: 2120 1772 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5410 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2134 3178 3320 4865 1132 Size: 97 905 1044 142 1545 2229 Fragments arranged by size: 2229 1545 1044 905 142 97 PasI CC'CwG_GG Cuts at: 1348 2190 1348 Size: 842 5120 PciI A'CATG_T Cuts at: 2130 4861 2130 Size: 2731 3231 PflMI CCAn_nnn'nTGG Cuts at: 1108 3070 1108 Size: 1962 4000 PfoI T'CCnGG_A Cuts at: 2877 2877 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2585 1613 Size: 972 4990 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PstI C_TGCA'G Cuts at: 830 2436 2490 3314 830 Size: 1606 54 824 3478 Fragments arranged by size: 3478 1606 824 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2432 2486 3310 4445 5126 5317 826 Size: 219 1387 54 824 1135 681 191 1471 Fragments arranged by size: 1471 1387 1135 824 681 219 191 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2894 2966 3218 4066 4967 5229 Size: 197 926 951 72 252 848 901 262 Cuts at: 5229 820 Size: 1553 Fragments arranged by size: 1553 951 926 901 848 262 252 197 72 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 2373 1517 Size: 856 5106 StyI C'CwwG_G Cuts at: 513 1065 3401 513 Size: 552 2336 3074 Fragments arranged by size: 3074 2336 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3989 4006 4159 4344 4789 325 Size: 1255 2409 17 153 185 445 1498 Fragments arranged by size: 2409 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4208 5920 96 Size: 274 80 33 51 1443 2231 1712 138 Fragments arranged by size: 2231 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5922 5954 5922 Size: 32 5930 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2080 2080 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 2015 2156 4091 2015 Size: 141 1935 3886 Fragments arranged by size: 3886 1935 141 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI