(Circular) (Six-base) MAPSORT of: pFN21AB6637.seq Check: 693 from: 1 to: 5959 pFN21AB6637 5959 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2164 2468 2993 2995 3077 3132 4022 5406 Size: 532 304 525 2 82 55 890 1384 Cuts at: 5406 5674 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 890 532 525 304 268 82 55 2 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 2920 4688 2920 Size: 1768 4191 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2051 2065 1184 Size: 867 14 5078 Fragments arranged by size: 5078 867 14 AlwNI CAG_nnn'CTG Cuts at: 2863 3702 5274 2863 Size: 839 1572 3548 Fragments arranged by size: 3548 1572 839 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 2317 3268 3494 3646 5755 1493 Size: 824 951 226 152 2109 1697 Fragments arranged by size: 2109 1697 951 824 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 3161 3284 1870 Size: 73 1218 123 4545 Fragments arranged by size: 4545 1218 123 73 BamHI G'GATC_C Cuts at: 2080 3289 3656 2080 Size: 1209 367 4383 Fragments arranged by size: 4383 1209 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2728 3049 3280 4735 618 Size: 325 217 627 941 321 231 1455 1842 Fragments arranged by size: 1842 1455 941 627 325 321 231 217 BanII G_rGCy'C Cuts at: 1391 3278 1391 Size: 1887 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2004 2111 2919 3001 3181 928 Size: 971 105 107 808 82 180 3706 Fragments arranged by size: 3706 971 808 180 107 105 82 BbvCI CC'TCA_GC Cuts at: 2076 2435 2076 Size: 359 5600 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4150 4184 1903 Size: 34 50 34 2129 34 3678 Fragments arranged by size: 3678 2129 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 2125 1548 Size: 577 5382 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4570 136 Size: 107 122 71 631 605 2898 1525 Fragments arranged by size: 2898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 4020 5176 1790 Size: 2230 1156 2573 Fragments arranged by size: 2573 2230 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2231 3002 3690 Size: 416 53 83 399 24 814 771 688 Cuts at: 3690 4648 442 Size: 958 1753 Fragments arranged by size: 1753 958 814 771 688 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2390 4619 1239 Size: 1088 63 2229 2579 Fragments arranged by size: 2579 2229 1088 63 Bpu10I CC'TnA_GC Cuts at: 2076 2435 2076 Size: 359 5600 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2556 4084 4949 5247 2556 Size: 1528 865 298 3268 Fragments arranged by size: 3268 1528 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2656 4622 882 Size: 617 1157 1966 2219 Fragments arranged by size: 2219 1966 1157 617 BsaAI yAC'GTr Cuts at: 493 1204 2557 2704 3750 493 Size: 711 1353 147 1046 2702 Fragments arranged by size: 2702 1353 1046 711 147 BsaBI GATnn'nnATC Cuts at: 2130 5953 2130 Size: 3823 2136 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3766 4148 4783 275 Size: 53 83 186 936 2233 382 635 1451 Fragments arranged by size: 2233 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2338 4392 4790 5064 5211 5622 2338 Size: 2054 398 274 147 411 2675 Fragments arranged by size: 2675 2054 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3202 3232 1178 Size: 30 165 30 1799 30 3905 Fragments arranged by size: 3905 1799 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 3059 1186 Size: 1177 696 4086 Fragments arranged by size: 4086 1177 696 BseYI C'CCAG_C Cuts at: 2787 3119 5162 5658 2787 Size: 332 2043 496 3088 Fragments arranged by size: 3088 2043 496 332 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5386 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4020 4105 5176 4020 Size: 85 1071 4803 Fragments arranged by size: 4803 1071 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3278 4020 4105 5176 1391 Size: 399 1488 742 85 1071 2174 Fragments arranged by size: 2174 1488 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2338 5622 2338 Size: 3284 2675 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3314 844 Size: 2470 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2268 2428 2821 3022 4454 4628 65 Size: 1183 1020 160 393 201 1432 174 1396 Fragments arranged by size: 1432 1396 1183 1020 393 201 174 160 BsrFI r'CCGG_y Cuts at: 1700 1810 2417 4603 4790 1700 Size: 110 607 2186 187 2869 Fragments arranged by size: 2869 2186 607 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 2020 4019 5031 2020 Size: 1999 1012 2948 Fragments arranged by size: 2948 1999 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 2713 1184 Size: 1529 4430 BstXI CCAn_nnnn'nTGG Cuts at: 2902 2902 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2080 2349 3289 3656 4041 4058 5510 5596 Size: 108 269 940 367 385 17 1452 86 Cuts at: 5596 5608 5954 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 940 385 367 346 269 108 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5407 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2230 487 Size: 683 56 1004 4216 Fragments arranged by size: 4216 1004 683 56 BtsI GCAGTG_nn' Cuts at: 3475 4269 4289 3475 Size: 794 20 5145 Fragments arranged by size: 5145 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 2647 3263 2647 Size: 616 5343 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3406 4295 5924 Size: 54 1425 50 275 18 1576 889 1629 Cuts at: 5924 8 Size: 43 Fragments arranged by size: 1629 1576 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2523 2685 2969 3230 3889 1353 Size: 1170 162 284 261 659 3423 Fragments arranged by size: 3423 1170 659 284 261 162 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2164 2327 2390 2468 2993 2995 3077 Size: 393 532 163 63 78 525 2 82 Cuts at: 3077 3132 4022 4619 5406 5674 1239 Size: 55 890 597 787 268 1524 Fragments arranged by size: 1524 890 787 597 532 525 393 268 163 82 78 63 55 2 EcoNI CCTnn'n_nnAGG Cuts at: 2075 2438 2075 Size: 363 5596 EcoO109I rG'GnC_Cy Cuts at: 2311 2443 2311 Size: 132 5827 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 EcoRV GAT'ATC Cuts at: 2887 2887 Size: 5959 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 5106 1678 Size: 3428 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2906 2938 4600 4632 4674 Size: 32 135 32 1334 32 1662 32 42 Cuts at: 4674 4706 1373 Size: 32 2626 Fragments arranged by size: 2626 1662 1334 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2512 2990 3303 3549 669 Size: 988 281 574 478 313 246 3079 Fragments arranged by size: 3079 988 574 478 313 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2235 5073 5257 1305 Size: 930 2838 184 2007 Fragments arranged by size: 2838 2007 930 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4137 Fragments arranged by size: 4137 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3146 3917 4276 4435 518 Size: 648 1980 771 359 159 2042 Fragments arranged by size: 2042 1980 771 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2197 2410 2787 3375 4052 5200 5445 1606 Size: 591 213 377 588 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 591 588 377 245 213 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5407 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 2755 3317 4862 1132 Size: 97 1526 562 1545 2229 Fragments arranged by size: 2229 1545 1526 562 97 PasI CC'CwG_GG Cuts at: 1348 2086 2272 1348 Size: 738 186 5035 Fragments arranged by size: 5035 738 186 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PfoI T'CCnGG_A Cuts at: 2304 2364 2901 2304 Size: 60 537 5362 Fragments arranged by size: 5362 537 60 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PmlI CAC'GTG Cuts at: 2704 2704 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3155 3187 4028 4060 5541 5573 3155 Size: 32 841 32 1481 32 3541 Fragments arranged by size: 3541 1481 841 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 3311 830 Size: 2481 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 2197 2410 2787 2197 Size: 213 377 5369 Fragments arranged by size: 5369 377 213 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SapI GCTCTTCn'nnn_ Cuts at: 2969 2969 Size: 5959 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SexAI A'CCwGG_T Cuts at: 2709 2709 Size: 5959 SfcI C'TryA_G Cuts at: 826 1045 2250 2855 3307 4442 5123 5314 826 Size: 219 1205 605 452 1135 681 191 1471 Fragments arranged by size: 1471 1205 1135 681 605 452 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2571 4063 4964 5226 820 Size: 197 926 628 1492 901 262 1553 Fragments arranged by size: 1553 1492 926 901 628 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 2046 1517 Size: 529 5430 StyI C'CwwG_G Cuts at: 513 1065 2280 3066 3398 513 Size: 552 1215 786 332 3074 Fragments arranged by size: 3074 1215 786 552 332 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2151 2779 2894 3266 3986 4003 4156 Size: 1255 571 628 115 372 720 17 153 Cuts at: 4156 4341 4786 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 720 628 571 445 372 185 153 115 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4205 5917 96 Size: 274 80 33 51 1443 2228 1712 138 Fragments arranged by size: 2228 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2320 2352 3225 3257 5919 5951 2320 Size: 32 873 32 2662 32 2328 Fragments arranged by size: 2662 2328 873 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI