Restriction map for
pFN21AB6623
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HpaI KasI KpnI
KspI MamI MflI MluI MspA1I
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 10 CGCG CG^CG
AccIII 1199 TCCGGA T^CCGGA
AfaI 83 GTAC GT^AC
Asp700I 109 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 218 TGGCCA TGG^CCA
BfaI 691 CTAG C^TAG
BglI 220 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 778 CTGGAG CTGGAG(16/14)
BsaWI 1199 TCCGGA [AT]^CCGG[AT]
BseAI 1199 TCCGGA T^CCGGA
BseRI 1102 GAGGAG GAGGAG(10/8)
BspEI 1199 TCCGGA T^CCGGA
BspMI 602 ACCTGC ACCTGC(4/8)
BsrDI 513 CATTGC GCAATG(2/0)
BstUI 10 CGCG CG^CG
CfoI 909 GCGC GCG^C
DsaI 1016 CCATGG C^C[GA][CT]GG
EaeI 218 TGGCCA [CT]^GGCC[GA]
EarI 901 GAAGAG CTCTTC(1/4)
FauI 156 CCCGC CCCGC(4/6)
HaeII 908 AGCGCC [GA]GCGC^[CT]
HapII 1200 CCGG C^CGG
HgaI 430 GCGTC GACGC(5/10)
HhaI 909 GCGC GCG^C
HinP1I 909 GCGC G^CGC
HincII 367 GTCAAC GT[CT]^[GA]AC
HindII 367 GTCAAC GT[CT]^[GA]AC
HindIII 253 AAGCTT A^AGCTT
HpaII 1200 CCGG C^CGG
Ksp632I 901 GAAGAG CTCTTC(1/4)
MaeI 691 CTAG C^TAG
MaeII 164 ACGT A^CGT
MfeI 203 CAATTG C^AATTG
MluNI 218 TGGCCA TGG^CCA
MroI 1199 TCCGGA T^CCGGA
MscI 218 TGGCCA TGG^CCA
MseI 197 TTAA T^TAA
MspI 1200 CCGG C^CGG
MunI 203 CAATTG C^AATTG
MvnI 10 CGCG CG^CG
NcoI 1016 CCATGG C^CATGG
NlaIV 1196 GGGTCC GG[ACGT]^[ACGT]CC
PflMI 210 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 700 CTGCAG CTGCA^G
RsaI 83 GTAC GT^AC
SfcI 700 CTGCAG C^T[GA][CT]AG
TfiI 967 GAATC G^A[AT]TC
Tru9I 197 TTAA T^TAA
Tsp45I 1226 GTGAC ^GT[GC]AC
TspRI 975 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 210 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 109 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 75 AAATTT [GA]^AATT[CT]
784 AAATTT
ApoI 75 AAATTT [GA]^AATT[CT]
784 AAATTT
AvaII 180 GGACC G^G[AT]CC
1197 GGTCC
BciVI 49 GGATAC GTATCC(6/5)
168 GGATAC
BsrI 669 ACTGG ACTGG(1/-1)
210 CCAGT
BstXI 329 CCAAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
518 CCAGACCTCTGG
Cac8I 426 GCTTGC GC[ACGT]^[ACGT]GC
911 GCCTGC
Cfr13I 180 GGACC G^G[ACGT]CC
1197 GGTCC
DdeI 248 CTAAG C^T[ACGT]AG
743 CTGAG
DpnII 297 GATC ^GATC
385 GATC
HphI 440 TCACC GGTGA(8/7)
1174 TCACC
MaeIII 929 GTAAC ^GT[ACGT]AC
1226 GTGAC
MboI 297 GATC ^GATC
385 GATC
MslI 27 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1017 CATGGAGATG
NdeII 297 GATC ^GATC
385 GATC
Sau3AI 297 GATC ^GATC
385 GATC
Sau96I 180 GGACC G^G[ACGT]CC
1197 GGTCC
SmlI 892 CTCAAG C^T[CT][GA]AG
916 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI BanII BbsI BbvI
BmyI BpuAI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MboII MnlI MvaI
MwoI NlaIII PleI ScrFI SfaNI
StyI TaqI TseI Tsp509I TthHB8I