(Circular) (Six-base) MAPSORT of: pFN21AB6623.seq Check: 8973 from: 1 to: 5944 pFN21AB6623 5944 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3754 278 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3287 1937 Size: 1350 4594 Acc65I G'GTAC_C Cuts at: 3265 3265 Size: 5944 AclI AA'CG_TT Cuts at: 1563 4071 4444 1563 Size: 2508 373 3063 Fragments arranged by size: 3063 2508 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2133 2318 4007 5391 5659 1632 Size: 501 185 1689 1384 268 1917 Fragments arranged by size: 1917 1689 1384 501 268 185 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5747 AflIII A'CryG_T Cuts at: 1203 4843 5820 1203 Size: 3640 977 1327 Fragments arranged by size: 3640 1327 977 AgeI A'CCGG_T Cuts at: 4775 4775 Size: 5944 AhdI GACnn_n'nnGTC Cuts at: 4673 4673 Size: 5944 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5944 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5944 AlwNI CAG_nnn'CTG Cuts at: 3687 5259 3687 Size: 1572 4372 ApaLI G'TGCA_C Cuts at: 4001 5157 4001 Size: 1156 4788 ApoI r'AATT_y Cuts at: 1493 2080 2789 3253 3479 3631 5740 1493 Size: 587 709 464 226 152 2109 1697 Fragments arranged by size: 2109 1697 709 587 464 226 152 AseI AT'TA_AT Cuts at: 160 1032 4498 160 Size: 872 3466 1606 Fragments arranged by size: 3466 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 AvaI C'yCGr_G Cuts at: 1870 1943 3269 1870 Size: 73 1326 4545 Fragments arranged by size: 4545 1326 73 BamHI G'GATC_C Cuts at: 3274 3641 3274 Size: 367 5577 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3265 4720 618 Size: 325 217 627 1478 1455 1842 Fragments arranged by size: 1842 1478 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2050 2148 2351 3263 1391 Size: 659 98 203 912 4072 Fragments arranged by size: 4072 912 659 203 98 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2336 2603 2750 2832 3049 3165 928 Size: 971 437 267 147 82 217 116 3707 Fragments arranged by size: 3707 971 437 267 217 147 116 82 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4135 4169 1903 Size: 34 2198 34 3678 Fragments arranged by size: 3678 2198 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2048 2167 3836 5046 2048 Size: 119 1669 1210 2946 Fragments arranged by size: 2946 1669 1210 119 BclI T'GATC_A Cuts at: 1548 1548 Size: 5944 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2231 4555 136 Size: 107 122 71 631 605 559 2324 1525 Fragments arranged by size: 2324 1525 631 605 559 122 107 71 BglII A'GATC_T Cuts at: 5939 5939 Size: 5944 BlpI GC'TnA_GC Cuts at: 3361 3361 Size: 5944 Bme1580I G_kGCm'C Cuts at: 1790 3068 4005 5161 1790 Size: 1278 937 1156 2573 Fragments arranged by size: 2573 1278 1156 937 BmgBI CAC'GTC Cuts at: 1318 2170 1318 Size: 852 5092 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2209 3675 4633 Size: 416 53 83 399 24 792 1466 958 Cuts at: 4633 442 Size: 1753 Fragments arranged by size: 1753 1466 958 792 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5944 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3189 3189 Size: 5944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2804 4604 1239 Size: 1565 1800 2579 Fragments arranged by size: 2579 1800 1565 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2882 2906 4069 4934 5232 2882 Size: 24 1163 865 298 3594 Fragments arranged by size: 3594 1163 865 298 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4607 882 Size: 617 3108 2219 Fragments arranged by size: 3108 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3735 493 Size: 711 2531 2702 Fragments arranged by size: 2702 2531 711 BsaBI GATnn'nnATC Cuts at: 5938 5938 Size: 5944 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3751 4133 4768 275 Size: 53 83 186 936 2218 382 635 1451 Fragments arranged by size: 2218 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3204 4377 4775 5049 5196 5607 3204 Size: 1173 398 274 147 411 3541 Fragments arranged by size: 3541 1173 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5719 Fragments arranged by size: 5719 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3122 1186 Size: 1936 4008 BseYI C'CCAG_C Cuts at: 5147 5643 5147 Size: 496 5448 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5371 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3394 4155 5183 1540 Size: 275 186 1393 761 1028 2301 Fragments arranged by size: 2301 1393 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4005 4090 5161 4005 Size: 85 1071 4788 Fragments arranged by size: 4788 1071 85 BsmI GAATG_Cn' Cuts at: 3455 3548 3455 Size: 93 5851 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2148 2351 3068 3263 4005 4090 Size: 399 260 98 203 717 195 742 85 Cuts at: 4090 5161 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 717 399 260 203 195 98 85 BspEI T'CCGG_A Cuts at: 3204 5607 3204 Size: 2403 3541 BspHI T'CATG_A Cuts at: 3833 5563 3833 Size: 1730 4214 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2616 3299 844 Size: 1772 683 3489 Fragments arranged by size: 3489 1772 683 BsrBI CCG'CTC Cuts at: 1394 1823 3831 1394 Size: 429 2008 3507 Fragments arranged by size: 3507 2008 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2517 4439 4613 65 Size: 1183 1269 1922 174 1396 Fragments arranged by size: 1922 1396 1269 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4588 4775 1700 Size: 110 2778 187 2869 Fragments arranged by size: 2869 2778 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5944 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5944 BssSI C'ACGA_G Cuts at: 4004 5016 4004 Size: 1012 4932 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5944 BstBI TT'CG_AA Cuts at: 4748 4748 Size: 5944 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5944 BstXI CCAn_nnnn'nTGG Cuts at: 2341 2530 2341 Size: 189 5755 BstYI r'GATC_y Cuts at: 1972 3274 3641 4026 4043 5495 5581 5593 5939 Size: 1302 367 385 17 1452 86 12 346 Cuts at: 5939 1972 Size: 1977 Fragments arranged by size: 1977 1452 1302 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3021 513 Size: 552 1956 3436 Fragments arranged by size: 3436 1956 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5205 Fragments arranged by size: 5205 683 56 BtsI GCAGTG_nn' Cuts at: 3460 4254 4274 3460 Size: 794 20 5130 Fragments arranged by size: 5130 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5944 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5909 DraI TTT'AAA Cuts at: 3248 3248 Size: 5944 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4951 809 Size: 507 3635 1802 Fragments arranged by size: 3635 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 3391 4280 Size: 54 1425 50 275 18 393 1168 889 Cuts at: 4280 5909 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1168 889 393 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3391 1537 Size: 275 1579 4090 Fragments arranged by size: 4090 1579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2901 3874 1353 Size: 1548 973 3423 Fragments arranged by size: 3423 1548 973 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4531 4905 5051 387 Size: 1064 138 2942 374 146 1280 Fragments arranged by size: 2942 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2133 2318 2804 4007 4604 5391 5659 Size: 393 501 185 486 1203 597 787 268 Cuts at: 5659 1239 Size: 1524 Fragments arranged by size: 1524 1203 787 597 501 486 393 268 185 EcoRI G'AATT_C Cuts at: 3253 3631 3253 Size: 378 5566 FspI TGC'GCA Cuts at: 4450 4450 Size: 5944 HaeII r_GCGC'y Cuts at: 1678 2917 5091 1678 Size: 1239 2174 2531 Fragments arranged by size: 2531 2174 1239 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 2957 2989 4585 Size: 32 135 32 809 32 544 32 1596 Cuts at: 4585 4617 4659 4691 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1596 809 544 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2374 3288 3534 669 Size: 988 281 436 914 246 3079 Fragments arranged by size: 3079 988 914 436 281 246 HindIII A'AGCT_T Cuts at: 748 2258 748 Size: 1510 4434 HpaI GTT'AAC Cuts at: 3534 3534 Size: 5944 KpnI G_GTAC'C Cuts at: 3269 3269 Size: 5944 MfeI C'AATT_G Cuts at: 2208 3543 2208 Size: 1335 4609 MluI A'CGCG_T Cuts at: 5820 5820 Size: 5944 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2967 3000 5058 5242 1305 Size: 1662 33 2058 184 2007 Fragments arranged by size: 2058 2007 1662 184 33 MscI TGG'CCA Cuts at: 10 64 1489 1832 2225 10 Size: 54 1425 343 393 3729 Fragments arranged by size: 3729 1425 393 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 3026 3902 4261 4420 518 Size: 648 870 990 876 359 159 2042 Fragments arranged by size: 2042 990 876 870 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3360 4037 5185 5430 1606 Size: 1754 677 1148 245 2120 Fragments arranged by size: 2120 1754 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3021 513 Size: 552 1956 3436 Fragments arranged by size: 3436 1956 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5944 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5944 NotI GC'GGCC_GC Cuts at: 3391 3391 Size: 5944 NspI r_CATG'y Cuts at: 1132 1229 3302 4847 1132 Size: 97 2073 1545 2229 Fragments arranged by size: 2229 2073 1545 97 PasI CC'CwG_GG Cuts at: 1348 2050 1348 Size: 702 5242 PciI A'CATG_T Cuts at: 4843 4843 Size: 5944 PflMI CCAn_nnn'nTGG Cuts at: 1108 2221 1108 Size: 1113 4831 PfoI T'CCnGG_A Cuts at: 2958 2958 Size: 5944 PmeI GTTT'AAAC Cuts at: 3248 3248 Size: 5944 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4013 4045 5526 5558 4013 Size: 32 1481 32 4399 Fragments arranged by size: 4399 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5944 PsiI TTA'TAA Cuts at: 2319 3514 2319 Size: 1195 4749 PstI C_TGCA'G Cuts at: 830 2709 3296 830 Size: 1879 587 3478 Fragments arranged by size: 3478 1879 587 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5944 SalI G'TCGA_C Cuts at: 1936 3286 1936 Size: 1350 4594 SbfI CC_TGCA'GG Cuts at: 3296 3296 Size: 5944 ScaI AGT'ACT Cuts at: 4192 5904 4192 Size: 1712 4232 SfcI C'TryA_G Cuts at: 826 1045 2705 3292 4427 5108 5299 826 Size: 219 1660 587 1135 681 191 1471 Fragments arranged by size: 1660 1471 1135 681 587 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5944 SmaI CCC'GGG Cuts at: 1872 3271 1872 Size: 1399 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2897 2921 4048 4949 5211 820 Size: 197 926 954 24 1127 901 262 1553 Fragments arranged by size: 1553 1127 954 926 901 262 197 24 SnaBI TAC'GTA Cuts at: 493 3735 493 Size: 3242 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5944 SphI G_CATG'C Cuts at: 1132 3302 1132 Size: 2170 3774 SspI AAT'ATT Cuts at: 5 52 3868 5 Size: 47 3816 2081 Fragments arranged by size: 3816 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5944 StyI C'CwwG_G Cuts at: 513 1065 2229 2334 3021 3383 513 Size: 552 1164 105 687 362 3074 Fragments arranged by size: 3074 1164 687 552 362 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2107 2202 3971 3988 4141 4326 4771 Size: 1255 527 95 1769 17 153 185 445 Cuts at: 4771 325 Size: 1498 Fragments arranged by size: 1769 1498 1255 527 445 185 153 95 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4190 5902 96 Size: 274 80 33 51 1443 2213 1712 138 Fragments arranged by size: 2213 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5904 5936 5904 Size: 32 5912 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5944 XbaI T'CTAG_A Cuts at: 3280 3280 Size: 5944 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5944 XmaI C'CCGG_G Cuts at: 1870 3269 1870 Size: 1399 4545 XmnI GAAnn'nnTTC Cuts at: 2118 4073 2118 Size: 1955 3989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3752 276 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI