(Circular) (Six-base) MAPSORT of: pFN21AB6613.seq Check: 8043 from: 1 to: 5941 pFN21AB6613 5941 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2271 3284 1937 Size: 334 1013 4594 Fragments arranged by size: 4594 1013 334 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4004 5388 5656 1632 Size: 2372 1384 268 1917 Fragments arranged by size: 2372 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 2036 1166 Size: 870 5071 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2054 2453 1184 Size: 870 399 4672 Fragments arranged by size: 4672 870 399 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2954 2986 2954 Size: 32 5909 AlwNI CAG_nnn'CTG Cuts at: 3684 5256 3684 Size: 1572 4369 ApaI G_GGCC'C Cuts at: 3138 3138 Size: 5941 ApaLI G'TGCA_C Cuts at: 2126 3218 3998 5154 2126 Size: 1092 780 1156 2913 Fragments arranged by size: 2913 1156 1092 780 ApoI r'AATT_y Cuts at: 1493 2111 3250 3476 3628 5737 1493 Size: 618 1139 226 152 2109 1697 Fragments arranged by size: 2109 1697 1139 618 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2265 2474 2611 3191 3266 1870 Size: 73 83 239 209 137 580 75 4545 Fragments arranged by size: 4545 580 239 209 137 83 75 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 2900 3033 3262 4717 Size: 325 217 627 243 870 133 229 1455 Cuts at: 4717 618 Size: 1842 Fragments arranged by size: 1842 1455 870 627 325 243 229 217 133 BanII G_rGCy'C Cuts at: 1391 2066 2266 3138 3260 1391 Size: 675 200 872 122 4072 Fragments arranged by size: 4072 872 675 200 122 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4970 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2410 3833 5043 2410 Size: 1423 1210 3308 Fragments arranged by size: 3308 1423 1210 BclI T'GATC_A Cuts at: 1548 2659 1548 Size: 1111 4830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4552 136 Size: 107 122 71 631 605 2880 1525 Fragments arranged by size: 2880 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2406 5936 2406 Size: 3530 2411 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2033 2130 3038 3138 3222 4002 5158 1790 Size: 243 97 908 100 84 780 1156 2573 Fragments arranged by size: 2573 1156 908 780 243 100 97 84 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2642 3672 4630 Size: 416 53 83 399 24 1225 1030 958 Cuts at: 4630 442 Size: 1753 Fragments arranged by size: 1753 1225 1030 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2428 2428 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2462 2825 4601 1239 Size: 1223 363 1776 2579 Fragments arranged by size: 2579 1776 1223 363 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4066 4931 5229 4066 Size: 865 298 4778 Fragments arranged by size: 4778 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3147 4604 882 Size: 617 1648 1457 2219 Fragments arranged by size: 2219 1648 1457 617 BsaAI yAC'GTr Cuts at: 493 1204 2206 2641 3732 493 Size: 711 1002 435 1091 2702 Fragments arranged by size: 2702 1091 1002 711 435 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3000 3748 4130 4765 Size: 53 83 186 936 1467 748 382 635 Cuts at: 4765 275 Size: 1451 Fragments arranged by size: 1467 1451 936 748 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2505 2826 4374 4772 5046 5193 5604 2505 Size: 321 1548 398 274 147 411 2842 Fragments arranged by size: 2842 1548 411 398 321 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2177 2207 1178 Size: 30 165 30 774 30 4912 Fragments arranged by size: 4912 774 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3119 1186 Size: 1933 4008 BseYI C'CCAG_C Cuts at: 2978 3037 5144 5640 2978 Size: 59 2107 496 3279 Fragments arranged by size: 3279 2107 496 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2728 3257 1163 Size: 573 992 529 3847 Fragments arranged by size: 3847 992 573 529 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 2827 3095 3391 4152 5180 Size: 275 186 21 805 268 296 761 1028 Cuts at: 5180 1540 Size: 2301 Fragments arranged by size: 2301 1028 805 761 296 275 268 186 21 BsiHKAI G_wGCw'C Cuts at: 2130 2304 2432 3222 4002 4087 5158 2130 Size: 174 128 790 780 85 1071 2913 Fragments arranged by size: 2913 1071 790 780 174 128 85 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 BsmBI CGTCTCn'nnnn_ Cuts at: 2729 2729 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2066 2130 2266 2304 2432 3038 Size: 399 243 33 64 136 38 128 606 Cuts at: 3038 3138 3222 3260 4002 4087 5158 1391 Size: 100 84 38 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 606 399 243 136 128 100 85 84 64 38 38 33 BspEI T'CCGG_A Cuts at: 2505 5604 2505 Size: 3099 2842 BspHI T'CATG_A Cuts at: 2335 3830 5560 2335 Size: 1495 1730 2716 Fragments arranged by size: 2716 1730 1495 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2097 3188 3229 3296 844 Size: 1253 1091 41 67 3489 Fragments arranged by size: 3489 1253 1091 67 41 BsrBI CCG'CTC Cuts at: 1394 1823 3828 1394 Size: 429 2005 3507 Fragments arranged by size: 3507 2005 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2520 4436 4610 65 Size: 1183 1272 1916 174 1396 Fragments arranged by size: 1916 1396 1272 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4585 4772 1700 Size: 110 2775 187 2869 Fragments arranged by size: 2869 2775 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 2531 794 Size: 1737 4204 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstXI CCAn_nnnn'nTGG Cuts at: 2791 3082 2791 Size: 291 5650 BstYI r'GATC_y Cuts at: 1972 2406 3271 3638 4023 4040 5492 5578 5590 Size: 434 865 367 385 17 1452 86 12 Cuts at: 5590 5936 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 865 434 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 513 Size: 552 939 30 4420 Fragments arranged by size: 4420 939 552 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2258 487 Size: 683 56 1032 4170 Fragments arranged by size: 4170 1032 683 56 BtsI GCAGTG_nn' Cuts at: 3457 4251 4271 3457 Size: 794 20 5127 Fragments arranged by size: 5127 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 2371 3245 2371 Size: 874 5067 DraIII CAC_nnn'GTG Cuts at: 3033 3033 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2216 4948 809 Size: 507 900 2732 1802 Fragments arranged by size: 2732 1802 900 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2052 3388 Size: 54 1425 50 275 18 189 33 1336 Cuts at: 3388 4277 5906 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1336 889 275 189 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2019 3388 1537 Size: 275 207 1369 4090 Fragments arranged by size: 4090 1369 275 207 EarI CTCTTCn'nnn_ Cuts at: 1353 2075 2864 3871 1353 Size: 722 789 1007 3423 Fragments arranged by size: 3423 1007 789 722 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2462 2825 4004 4601 5388 5656 1239 Size: 393 830 363 1179 597 787 268 1524 Fragments arranged by size: 1524 1179 830 787 597 393 363 268 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2161 2875 2161 Size: 714 5227 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 5088 1678 Size: 3410 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2708 2740 4582 4614 4656 Size: 32 135 32 1136 32 1842 32 42 Cuts at: 4656 4688 1373 Size: 32 2626 Fragments arranged by size: 2626 1842 1136 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 2320 748 Size: 1572 4369 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2796 3075 5055 5239 1305 Size: 1491 279 1980 184 2007 Fragments arranged by size: 2007 1980 1491 279 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4119 Fragments arranged by size: 4119 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 2435 3059 3213 3899 4258 4417 Size: 648 870 399 624 154 686 359 159 Cuts at: 4417 518 Size: 2042 Fragments arranged by size: 2042 870 686 648 624 399 359 159 154 MspA1I CmG'CkG Cuts at: 1606 2036 3357 4034 5182 5427 1606 Size: 430 1321 677 1148 245 2120 Fragments arranged by size: 2120 1321 1148 677 430 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4450 Fragments arranged by size: 4450 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 2019 3388 2019 Size: 1369 4572 NspI r_CATG'y Cuts at: 1132 1229 3299 4844 1132 Size: 97 2070 1545 2229 Fragments arranged by size: 2229 2070 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PmlI CAC'GTG Cuts at: 2206 2206 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 3134 3134 Size: 5941 PspXI vC'TCGA_Gb Cuts at: 2265 2265 Size: 5941 PstI C_TGCA'G Cuts at: 830 2094 3293 830 Size: 1264 1199 3478 Fragments arranged by size: 3478 1264 1199 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 RsrII CG'GwC_CG Cuts at: 2767 2767 Size: 5941 SacII CC_GC'GG Cuts at: 2037 2037 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SapI GCTCTTCn'nnn_ Cuts at: 2075 2075 Size: 5941 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SexAI A'CCwGG_T Cuts at: 2118 2118 Size: 5941 SfcI C'TryA_G Cuts at: 826 1045 2090 3289 4424 5105 5296 826 Size: 219 1045 1199 1135 681 191 1471 Fragments arranged by size: 1471 1199 1135 1045 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2028 3193 3268 1872 Size: 156 1165 75 4545 Fragments arranged by size: 4545 1165 156 75 SmlI C'TyrA_G Cuts at: 820 1017 1943 2265 4045 4946 5208 820 Size: 197 926 322 1780 901 262 1553 Fragments arranged by size: 1780 1553 926 901 322 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 2192 1517 Size: 675 5266 StyI C'CwwG_G Cuts at: 513 1065 2004 3075 3380 513 Size: 552 939 1071 305 3074 Fragments arranged by size: 3074 1071 939 552 305 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3968 3985 4138 4323 4768 325 Size: 1255 2388 17 153 185 445 1498 Fragments arranged by size: 2388 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2607 3018 4187 Size: 274 80 33 51 1443 630 411 1169 Cuts at: 4187 5899 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1169 630 411 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2203 2728 3145 2203 Size: 525 417 4999 Fragments arranged by size: 4999 525 417 XhoI C'TCGA_G Cuts at: 1943 2265 1943 Size: 322 5619 XmaI C'CCGG_G Cuts at: 1870 2026 3191 3266 1870 Size: 156 1165 75 4545 Fragments arranged by size: 4545 1165 156 75 XmnI GAAnn'nnTTC Cuts at: 2858 4070 2858 Size: 1212 4729 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PsrI PvuII SacI SanDI SfiI SfoI SrfI SwaI