(Circular) (Six-base) MAPSORT of: pFN21AB6607.seq Check: 5146 from: 1 to: 5932 pFN21AB6607 5932 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2380 2428 2380 Size: 48 5884 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3742 278 Size: 53 83 186 936 2206 2468 Fragments arranged by size: 2468 2206 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3275 1937 Size: 1338 4594 Acc65I G'GTAC_C Cuts at: 3253 3253 Size: 5932 AclI AA'CG_TT Cuts at: 1563 4059 4432 1563 Size: 2496 373 3063 Fragments arranged by size: 3063 2496 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 2745 2930 3162 3995 5379 5647 1632 Size: 407 706 185 232 833 1384 268 1917 Fragments arranged by size: 1917 1384 833 706 407 268 232 185 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5735 AflIII A'CryG_T Cuts at: 1203 4831 5808 1203 Size: 3628 977 1327 Fragments arranged by size: 3628 1327 977 AgeI A'CCGG_T Cuts at: 4763 4763 Size: 5932 AhdI GACnn_n'nnGTC Cuts at: 4661 4661 Size: 5932 AleI CACnn'nnGTG Cuts at: 1166 2321 1166 Size: 1155 4777 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2643 1184 Size: 1459 4473 AlwNI CAG_nnn'CTG Cuts at: 2282 2495 2771 3059 3675 5247 2282 Size: 213 276 288 616 1572 2967 Fragments arranged by size: 2967 1572 616 288 276 213 ApaLI G'TGCA_C Cuts at: 2550 3989 5145 2550 Size: 1439 1156 3337 Fragments arranged by size: 3337 1439 1156 ApoI r'AATT_y Cuts at: 1493 2834 3187 3241 3467 3619 5728 1493 Size: 1341 353 54 226 152 2109 1697 Fragments arranged by size: 2109 1697 1341 353 226 152 54 AseI AT'TA_AT Cuts at: 160 1032 2141 4486 160 Size: 872 1109 2345 1606 Fragments arranged by size: 2345 1606 1109 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 AvaI C'yCGr_G Cuts at: 1870 1943 2060 3257 1870 Size: 73 117 1197 4545 Fragments arranged by size: 4545 1197 117 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3146 3179 3146 Size: 33 5899 BamHI G'GATC_C Cuts at: 3262 3629 3262 Size: 367 5565 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3253 4708 618 Size: 325 217 627 1466 1455 1842 Fragments arranged by size: 1842 1466 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2752 3251 1391 Size: 1361 499 4072 Fragments arranged by size: 4072 1361 499 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2027 2751 928 Size: 971 128 724 4109 Fragments arranged by size: 4109 971 724 128 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2906 2940 4123 4157 1903 Size: 34 50 34 885 34 1183 34 3678 Fragments arranged by size: 3678 1183 885 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3824 5034 3824 Size: 1210 4722 BclI T'GATC_A Cuts at: 1548 1548 Size: 5932 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4543 136 Size: 107 122 71 631 605 2871 1525 Fragments arranged by size: 2871 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5927 5927 Size: 5932 BlpI GC'TnA_GC Cuts at: 3349 3349 Size: 5932 Bme1580I G_kGCm'C Cuts at: 1790 2554 3993 5149 1790 Size: 764 1439 1156 2573 Fragments arranged by size: 2573 1439 1156 764 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5932 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2754 3663 4621 Size: 416 53 83 399 24 1337 909 958 Cuts at: 4621 442 Size: 1753 Fragments arranged by size: 1753 1337 958 909 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5932 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2012 2370 2715 2838 4592 1239 Size: 773 358 345 123 1754 2579 Fragments arranged by size: 2579 1754 773 358 345 123 Bpu10I CC'TnA_GC Cuts at: 2328 2328 Size: 5932 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2715 4057 4922 5220 2715 Size: 1342 865 298 3427 Fragments arranged by size: 3427 1342 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4595 882 Size: 617 3096 2219 Fragments arranged by size: 3096 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3723 493 Size: 711 2519 2702 Fragments arranged by size: 2702 2519 711 BsaBI GATnn'nnATC Cuts at: 5926 5926 Size: 5932 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3739 4121 4756 275 Size: 53 83 186 936 2206 382 635 1451 Fragments arranged by size: 2206 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4365 4763 5037 5184 5595 4365 Size: 398 274 147 411 4702 Fragments arranged by size: 4702 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2734 2764 1178 Size: 30 165 30 1331 30 4346 Fragments arranged by size: 4346 1331 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2742 3036 3039 1186 Size: 892 664 294 3 4079 Fragments arranged by size: 4079 892 664 294 3 BseYI C'CCAG_C Cuts at: 5135 5631 5135 Size: 496 5436 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2400 2448 1163 Size: 573 664 48 4647 Fragments arranged by size: 4647 664 573 48 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3382 4143 5171 1540 Size: 275 186 1381 761 1028 2301 Fragments arranged by size: 2301 1381 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2554 3993 4078 5149 2554 Size: 1439 85 1071 3337 Fragments arranged by size: 3337 1439 1071 85 BsmI GAATG_Cn' Cuts at: 3443 3536 3443 Size: 93 5839 BsmBI CGTCTCn'nnnn_ Cuts at: 2060 2060 Size: 5932 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2554 2752 3251 3993 4078 5149 1391 Size: 399 764 198 499 742 85 1071 2174 Fragments arranged by size: 2174 1071 764 742 499 399 198 85 BspEI T'CCGG_A Cuts at: 5595 5595 Size: 5932 BspHI T'CATG_A Cuts at: 3821 5551 3821 Size: 1730 4202 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 1999 2120 2380 2422 2428 3287 844 Size: 1155 121 260 42 6 859 3489 Fragments arranged by size: 3489 1155 859 260 121 42 6 BsrBI CCG'CTC Cuts at: 1394 1823 3819 1394 Size: 429 1996 3507 Fragments arranged by size: 3507 1996 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4427 4601 65 Size: 1183 3179 174 1396 Fragments arranged by size: 3179 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4576 4763 1700 Size: 110 2766 187 2869 Fragments arranged by size: 2869 2766 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5932 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5932 BssSI C'ACGA_G Cuts at: 3992 5004 3992 Size: 1012 4920 BstAPI GCAn_nnn'nTGC Cuts at: 794 2549 2771 794 Size: 1755 222 3955 Fragments arranged by size: 3955 1755 222 BstBI TT'CG_AA Cuts at: 4736 4736 Size: 5932 BstEII G'GTnAC_C Cuts at: 1184 2011 2224 1184 Size: 827 213 4892 Fragments arranged by size: 4892 827 213 BstXI CCAn_nnnn'nTGG Cuts at: 2323 2323 Size: 5932 BstYI r'GATC_y Cuts at: 1972 3262 3629 4014 4031 5483 5569 5581 5927 Size: 1290 367 385 17 1452 86 12 346 Cuts at: 5927 1972 Size: 1977 Fragments arranged by size: 1977 1452 1290 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3096 3096 Size: 5932 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5380 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5193 Fragments arranged by size: 5193 683 56 BtsI GCAGTG_nn' Cuts at: 2247 2673 2772 3046 3448 4242 4262 2247 Size: 426 99 274 402 794 20 3917 Fragments arranged by size: 3917 794 426 402 274 99 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5932 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5897 DraI TTT'AAA Cuts at: 3236 3236 Size: 5932 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2566 4939 809 Size: 507 1250 2373 1802 Fragments arranged by size: 2373 1802 1250 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3379 4268 5897 Size: 54 1425 50 275 18 1549 889 1629 Cuts at: 5897 8 Size: 43 Fragments arranged by size: 1629 1549 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3379 1537 Size: 275 1567 4090 Fragments arranged by size: 4090 1567 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3862 1353 Size: 2509 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4519 4893 5039 387 Size: 1064 138 2930 374 146 1280 Fragments arranged by size: 2930 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2012 2039 2370 2715 2745 2838 2930 Size: 393 380 27 331 345 30 93 92 Cuts at: 2930 3162 3995 4592 5379 5647 1239 Size: 232 833 597 787 268 1524 Fragments arranged by size: 1524 833 787 597 393 380 345 331 268 232 93 92 30 27 EcoRI G'AATT_C Cuts at: 3241 3619 3241 Size: 378 5554 FspI TGC'GCA Cuts at: 4438 4438 Size: 5932 HaeII r_GCGC'y Cuts at: 1678 5079 1678 Size: 3401 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2489 2521 2582 2614 4573 Size: 32 135 32 917 32 61 32 1959 Cuts at: 4573 4605 4647 4679 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1959 917 135 61 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2722 3276 3522 669 Size: 988 281 784 554 246 3079 Fragments arranged by size: 3079 988 784 554 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5932 HpaI GTT'AAC Cuts at: 3522 3522 Size: 5932 KpnI G_GTAC'C Cuts at: 3257 3257 Size: 5932 MfeI C'AATT_G Cuts at: 3531 3531 Size: 5932 MluI A'CGCG_T Cuts at: 5808 5808 Size: 5932 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2360 5046 5230 1305 Size: 1055 2686 184 2007 Fragments arranged by size: 2686 2007 1055 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4110 Fragments arranged by size: 4110 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2321 2600 3890 4249 4408 518 Size: 648 1155 279 1290 359 159 2042 Fragments arranged by size: 2042 1290 1155 648 359 279 159 MspA1I CmG'CkG Cuts at: 1606 2777 3348 4025 5173 5418 1606 Size: 1171 571 677 1148 245 2120 Fragments arranged by size: 2120 1171 1148 677 571 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5380 NdeI CA'TA_TG Cuts at: 387 3072 387 Size: 2685 3247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5932 NotI GC'GGCC_GC Cuts at: 3379 3379 Size: 5932 NspI r_CATG'y Cuts at: 1132 1229 3290 4835 1132 Size: 97 2061 1545 2229 Fragments arranged by size: 2229 2061 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5932 PciI A'CATG_T Cuts at: 4831 4831 Size: 5932 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5932 PmeI GTTT'AAAC Cuts at: 3236 3236 Size: 5932 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4001 4033 5514 5546 4001 Size: 32 1481 32 4387 Fragments arranged by size: 4387 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5932 PsiI TTA'TAA Cuts at: 3502 3502 Size: 5932 PstI C_TGCA'G Cuts at: 830 2452 2776 2782 3284 830 Size: 1622 324 6 502 3478 Fragments arranged by size: 3478 1622 502 324 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5932 PvuII CAG'CTG Cuts at: 2777 2777 Size: 5932 SalI G'TCGA_C Cuts at: 1936 3274 1936 Size: 1338 4594 SbfI CC_TGCA'GG Cuts at: 3284 3284 Size: 5932 ScaI AGT'ACT Cuts at: 2743 4180 5892 2743 Size: 1437 1712 2783 Fragments arranged by size: 2783 1712 1437 SfcI C'TryA_G Cuts at: 826 1045 2292 2448 2772 2778 2912 3111 3209 Size: 219 1247 156 324 6 134 199 98 Cuts at: 3209 3280 4415 5096 5287 826 Size: 71 1135 681 191 1471 Fragments arranged by size: 1471 1247 1135 681 324 219 199 191 156 134 98 71 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5932 SmaI CCC'GGG Cuts at: 1872 3259 1872 Size: 1387 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2730 4036 4937 5199 820 Size: 197 926 787 1306 901 262 1553 Fragments arranged by size: 1553 1306 926 901 787 262 197 SnaBI TAC'GTA Cuts at: 493 3723 493 Size: 3230 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5932 SphI G_CATG'C Cuts at: 1132 3290 1132 Size: 2158 3774 SspI AAT'ATT Cuts at: 5 52 3856 5 Size: 47 3804 2081 Fragments arranged by size: 3804 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5932 StyI C'CwwG_G Cuts at: 513 1065 2352 3371 513 Size: 552 1287 1019 3074 Fragments arranged by size: 3074 1287 1019 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2511 3959 3976 4129 4314 4759 325 Size: 1255 931 1448 17 153 185 445 1498 Fragments arranged by size: 1498 1448 1255 931 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2501 2741 4178 Size: 274 80 33 51 1443 524 240 1437 Cuts at: 4178 5890 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1437 524 274 240 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5892 5924 5892 Size: 32 5900 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5932 XbaI T'CTAG_A Cuts at: 3268 3268 Size: 5932 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2320 2320 Size: 5932 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5932 XmaI C'CCGG_G Cuts at: 1870 3257 1870 Size: 1387 4545 XmnI GAAnn'nnTTC Cuts at: 2997 4061 2997 Size: 1064 4868 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3740 276 Size: 53 83 186 936 2206 2468 Fragments arranged by size: 2468 2206 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI