(Circular) (Six-base) MAPSORT of: pFN21AB6127.seq Check: 3794 from: 1 to: 6466 pFN21AB6127 6466 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3183 4276 278 Size: 53 83 186 936 1647 1093 2468 Fragments arranged by size: 2468 1647 1093 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3809 1937 Size: 1872 4594 Acc65I G'GTAC_C Cuts at: 3787 3787 Size: 6466 AclI AA'CG_TT Cuts at: 1563 4593 4966 1563 Size: 3030 373 3063 Fragments arranged by size: 3063 3030 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3599 4529 5913 6181 1632 Size: 1967 930 1384 268 1917 Fragments arranged by size: 1967 1917 1384 930 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6269 AflIII A'CryG_T Cuts at: 1203 2772 3129 5365 6342 1203 Size: 1569 357 2236 977 1327 Fragments arranged by size: 2236 1569 1327 977 357 AgeI A'CCGG_T Cuts at: 5297 5297 Size: 6466 AhdI GACnn_n'nnGTC Cuts at: 2773 5195 2773 Size: 2422 4044 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6466 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6466 AlwNI CAG_nnn'CTG Cuts at: 2629 2926 3238 4209 5781 2629 Size: 297 312 971 1572 3314 Fragments arranged by size: 3314 1572 971 312 297 ApaI G_GGCC'C Cuts at: 2104 3525 2104 Size: 1421 5045 ApaLI G'TGCA_C Cuts at: 4523 5679 4523 Size: 1156 5310 ApoI r'AATT_y Cuts at: 1493 3775 4001 4153 6262 1493 Size: 2282 226 152 2109 1697 Fragments arranged by size: 2282 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5020 160 Size: 872 3988 1606 Fragments arranged by size: 3988 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 AvaI C'yCGr_G Cuts at: 1870 1943 2097 2472 3062 3791 1870 Size: 73 154 375 590 729 4545 Fragments arranged by size: 4545 729 590 375 154 73 BamHI G'GATC_C Cuts at: 3796 4163 3796 Size: 367 6099 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2170 2840 3058 3161 3787 Size: 325 217 627 383 670 218 103 626 Cuts at: 3787 5242 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 670 627 626 383 325 218 217 103 BanII G_rGCy'C Cuts at: 1391 2104 2262 3372 3465 3525 3785 1391 Size: 713 158 1110 93 60 260 4072 Fragments arranged by size: 4072 1110 713 260 158 93 60 BbeI G_GCGC'C Cuts at: 2174 3062 3165 2174 Size: 888 103 5475 Fragments arranged by size: 5475 888 103 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2231 3090 928 Size: 971 332 859 4304 Fragments arranged by size: 4304 971 859 332 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4657 4691 1903 Size: 34 2720 34 3678 Fragments arranged by size: 3678 2720 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4358 5568 4358 Size: 1210 5256 BclI T'GATC_A Cuts at: 1548 2232 1548 Size: 684 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2313 2964 3390 Size: 107 122 71 631 605 641 651 426 Cuts at: 3390 5077 136 Size: 1687 1525 Fragments arranged by size: 1687 1525 651 641 631 605 426 122 107 71 BglII A'GATC_T Cuts at: 6461 6461 Size: 6466 BlpI GC'TnA_GC Cuts at: 3026 3883 3026 Size: 857 5609 Bme1580I G_kGCm'C Cuts at: 1790 2104 3525 4527 5683 1790 Size: 314 1421 1002 1156 2573 Fragments arranged by size: 2573 1421 1156 1002 314 BmgBI CAC'GTC Cuts at: 1318 3043 1318 Size: 1725 4741 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3616 4197 5155 Size: 416 53 83 399 24 2199 581 958 Cuts at: 5155 442 Size: 1753 Fragments arranged by size: 2199 1753 958 581 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6466 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2047 2633 3035 3409 5126 1239 Size: 808 586 402 374 1717 2579 Fragments arranged by size: 2579 1717 808 586 402 374 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2740 3320 4591 5456 5754 2740 Size: 580 1271 865 298 3452 Fragments arranged by size: 3452 1271 865 580 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5129 882 Size: 617 3630 2219 Fragments arranged by size: 3630 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3550 4257 493 Size: 711 2346 707 2702 Fragments arranged by size: 2702 2346 711 707 BsaBI GATnn'nnATC Cuts at: 6460 6460 Size: 6466 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2171 2847 2967 3059 Size: 53 83 186 936 638 676 120 92 Cuts at: 3059 3162 3180 3264 4273 4655 5290 275 Size: 103 18 84 1009 382 635 1451 Fragments arranged by size: 1451 1009 936 676 638 635 382 186 120 103 92 84 83 53 18 BsaWI w'CCGG_w Cuts at: 4899 5297 5571 5718 6129 4899 Size: 398 274 147 411 5236 Fragments arranged by size: 5236 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2062 2092 2634 2664 2876 Size: 30 165 30 659 30 542 30 212 Cuts at: 2876 2906 1178 Size: 30 4738 Fragments arranged by size: 4738 659 542 212 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2655 2825 1186 Size: 1469 170 4827 Fragments arranged by size: 4827 1469 170 BseYI C'CCAG_C Cuts at: 2163 3464 3513 5669 6165 2163 Size: 1301 49 2156 496 2464 Fragments arranged by size: 2464 2156 1301 496 49 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2040 2058 2270 2422 2555 2570 2867 Size: 573 304 18 212 152 133 15 297 Cuts at: 2867 2939 2963 3494 3581 1163 Size: 72 24 531 87 4048 Fragments arranged by size: 4048 573 531 304 297 212 152 133 87 72 24 18 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2991 3453 3916 4677 5705 1540 Size: 275 186 990 462 463 761 1028 2301 Fragments arranged by size: 2301 1028 990 761 463 462 275 186 BsiHKAI G_wGCw'C Cuts at: 4527 4612 5683 4527 Size: 85 1071 5310 Fragments arranged by size: 5310 1071 85 BsmI GAATG_Cn' Cuts at: 2668 3977 4070 2668 Size: 1309 93 5064 Fragments arranged by size: 5064 1309 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2262 3372 3465 3525 3785 4527 Size: 399 314 158 1110 93 60 260 742 Cuts at: 4527 4612 5683 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1110 1071 742 399 314 260 158 93 85 60 BspEI T'CCGG_A Cuts at: 6129 6129 Size: 6466 BspHI T'CATG_A Cuts at: 4355 6085 4355 Size: 1730 4736 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3420 3821 844 Size: 2576 401 3489 Fragments arranged by size: 3489 2576 401 BsrBI CCG'CTC Cuts at: 1394 1823 2485 2582 2637 3721 4353 1394 Size: 429 662 97 55 1084 632 3507 Fragments arranged by size: 3507 1084 662 632 429 97 55 BsrDI GCAATG_nn' Cuts at: 65 1248 2611 4961 5135 65 Size: 1183 1363 2350 174 1396 Fragments arranged by size: 2350 1396 1363 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2092 2932 3087 3409 5110 5297 1700 Size: 110 282 840 155 322 1701 187 2869 Fragments arranged by size: 2869 1701 840 322 282 187 155 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6466 BssHII G'CGCG_C Cuts at: 1925 3030 3032 3067 3069 3071 1925 Size: 1105 2 35 2 2 5320 Fragments arranged by size: 5320 1105 35 2 2 2 BssSI C'ACGA_G Cuts at: 2288 2588 3197 4526 5538 2288 Size: 300 609 1329 1012 3216 Fragments arranged by size: 3216 1329 1012 609 300 BstAPI GCAn_nnn'nTGC Cuts at: 794 2926 3238 794 Size: 2132 312 4022 Fragments arranged by size: 4022 2132 312 BstBI TT'CG_AA Cuts at: 3673 5270 3673 Size: 1597 4869 BstEII G'GTnAC_C Cuts at: 1184 2935 1184 Size: 1751 4715 BstYI r'GATC_y Cuts at: 1972 2625 3796 4163 4548 4565 6017 6103 6115 Size: 653 1171 367 385 17 1452 86 12 Cuts at: 6115 6461 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1171 653 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2569 3211 3247 3447 513 Size: 552 1504 642 36 200 3532 Fragments arranged by size: 3532 1504 642 552 200 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2223 2872 487 Size: 683 56 997 649 4081 Fragments arranged by size: 4081 997 683 649 56 BtsI GCAGTG_nn' Cuts at: 2070 2429 3982 4776 4796 2070 Size: 359 1553 794 20 3740 Fragments arranged by size: 3740 1553 794 359 20 ClaI AT'CG_AT Cuts at: 1554 3531 1554 Size: 1977 4489 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6431 DraI TTT'AAA Cuts at: 3770 3770 Size: 6466 DraIII CAC_nnn'GTG Cuts at: 2840 2840 Size: 6466 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2615 3611 5473 809 Size: 507 1299 996 1862 1802 Fragments arranged by size: 1862 1802 1299 996 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2305 2691 2988 Size: 54 1425 50 275 18 475 386 297 Cuts at: 2988 3201 3450 3913 4802 6431 8 Size: 213 249 463 889 1629 43 Fragments arranged by size: 1629 1425 889 475 463 386 297 275 249 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2988 3450 3913 1537 Size: 275 1176 462 463 4090 Fragments arranged by size: 4090 1176 463 462 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2208 4396 1353 Size: 855 2188 3423 Fragments arranged by size: 3423 2188 855 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3197 5053 5427 5573 387 Size: 1064 138 1608 1856 374 146 1280 Fragments arranged by size: 1856 1608 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2633 3035 3409 3599 4529 5126 Size: 393 415 586 402 374 190 930 597 Cuts at: 5126 5913 6181 1239 Size: 787 268 1524 Fragments arranged by size: 1524 930 787 597 586 415 402 393 374 268 190 EcoNI CCTnn'n_nnAGG Cuts at: 2372 2786 3427 2372 Size: 414 641 5411 Fragments arranged by size: 5411 641 414 EcoO109I rG'GnC_Cy Cuts at: 2101 3521 2101 Size: 1420 5046 EcoRI G'AATT_C Cuts at: 3775 4153 3775 Size: 378 6088 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2887 2887 Size: 6466 FspI TGC'GCA Cuts at: 3224 4972 3224 Size: 1748 4718 HaeII r_GCGC'y Cuts at: 1678 2174 3023 3062 3165 5613 1678 Size: 496 849 39 103 2448 2531 Fragments arranged by size: 2531 2448 849 496 103 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2582 2614 2759 2791 3476 Size: 32 135 32 1010 32 145 32 685 Cuts at: 3476 3508 5107 5139 5181 5213 1373 Size: 32 1599 32 42 32 2626 Fragments arranged by size: 2626 1599 1010 685 145 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3121 3810 4056 669 Size: 988 281 1183 689 246 3079 Fragments arranged by size: 3079 1183 988 689 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6466 HpaI GTT'AAC Cuts at: 4056 4056 Size: 6466 KasI G'GCGC_C Cuts at: 2170 3058 3161 2170 Size: 888 103 5475 Fragments arranged by size: 5475 888 103 KpnI G_GTAC'C Cuts at: 3791 3791 Size: 6466 MfeI C'AATT_G Cuts at: 4065 4065 Size: 6466 MluI A'CGCG_T Cuts at: 2772 6342 2772 Size: 3570 2896 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2487 2691 5580 5764 1305 Size: 1182 204 2889 184 2007 Fragments arranged by size: 2889 2007 1182 204 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2307 3203 10 Size: 54 1425 343 475 896 3273 Fragments arranged by size: 3273 1425 896 475 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3305 4424 4783 4942 518 Size: 648 2139 1119 359 159 2042 Fragments arranged by size: 2139 2042 1119 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2167 2284 2562 2571 2881 2977 3055 3235 Size: 561 117 278 9 310 96 78 180 Cuts at: 3235 3449 3513 3882 4559 5707 5952 1606 Size: 214 64 369 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 561 369 310 278 245 214 180 117 96 78 64 9 NaeI GCC'GGC Cuts at: 2094 3411 2094 Size: 1317 5149 NarI GG'CG_CC Cuts at: 2171 3059 3162 2171 Size: 888 103 5475 Fragments arranged by size: 5475 888 103 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5914 NdeI CA'TA_TG Cuts at: 387 387 Size: 6466 NgoMIV G'CCGG_C Cuts at: 2092 3409 2092 Size: 1317 5149 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6466 NotI GC'GGCC_GC Cuts at: 3913 3913 Size: 6466 NspI r_CATG'y Cuts at: 1132 1229 3824 5369 1132 Size: 97 2595 1545 2229 Fragments arranged by size: 2595 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2345 1348 Size: 997 5469 PciI A'CATG_T Cuts at: 5365 5365 Size: 6466 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6466 PfoI T'CCnGG_A Cuts at: 3540 3540 Size: 6466 PmeI GTTT'AAAC Cuts at: 3770 3770 Size: 6466 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4535 4567 6048 6080 4535 Size: 32 1481 32 4921 Fragments arranged by size: 4921 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6466 PsiI TTA'TAA Cuts at: 4036 4036 Size: 6466 PspOMI G'GGCC_C Cuts at: 2100 3521 2100 Size: 1421 5045 PstI C_TGCA'G Cuts at: 830 2334 2373 3818 830 Size: 1504 39 1445 3478 Fragments arranged by size: 3478 1504 1445 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6466 PvuII CAG'CTG Cuts at: 2167 3235 2167 Size: 1068 5398 SacII CC_GC'GG Cuts at: 2572 3450 2572 Size: 878 5588 SalI G'TCGA_C Cuts at: 1936 3808 1936 Size: 1872 4594 SbfI CC_TGCA'GG Cuts at: 2373 3818 2373 Size: 1445 5021 ScaI AGT'ACT Cuts at: 2030 4714 6426 2030 Size: 2684 1712 2070 Fragments arranged by size: 2684 2070 1712 SfcI C'TryA_G Cuts at: 826 1045 2330 2369 2908 3814 4949 5630 5821 Size: 219 1285 39 539 906 1135 681 191 Cuts at: 5821 826 Size: 1471 Fragments arranged by size: 1471 1285 1135 906 681 539 219 191 39 SfoI GGC'GCC Cuts at: 2172 3060 3163 2172 Size: 888 103 5475 Fragments arranged by size: 5475 888 103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6466 SmaI CCC'GGG Cuts at: 1872 2099 3793 1872 Size: 227 1694 4545 Fragments arranged by size: 4545 1694 227 SmlI C'TyrA_G Cuts at: 820 1017 1943 2719 3335 4570 5471 5733 820 Size: 197 926 776 616 1235 901 262 1553 Fragments arranged by size: 1553 1235 926 901 776 616 262 197 SnaBI TAC'GTA Cuts at: 493 4257 493 Size: 3764 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6466 SphI G_CATG'C Cuts at: 1132 3824 1132 Size: 2692 3774 SrfI GCCC'GGGC Cuts at: 2099 2099 Size: 6466 SspI AAT'ATT Cuts at: 5 52 4390 5 Size: 47 4338 2081 Fragments arranged by size: 4338 2081 47 StuI AGG'CCT Cuts at: 1517 2219 2717 2792 1517 Size: 702 498 75 5191 Fragments arranged by size: 5191 702 498 75 StyI C'CwwG_G Cuts at: 513 1065 2119 2646 3905 513 Size: 552 1054 527 1259 3074 Fragments arranged by size: 3074 1259 1054 552 527 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3776 4493 4510 4663 4848 5293 325 Size: 1255 2196 717 17 153 185 445 1498 Fragments arranged by size: 2196 1498 1255 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2028 3105 4712 Size: 274 80 33 51 1443 51 1077 1607 Cuts at: 4712 6424 96 Size: 1712 138 Fragments arranged by size: 1712 1607 1443 1077 274 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2062 2094 2634 2666 2876 2908 6426 6458 2062 Size: 32 540 32 210 32 3518 32 2070 Fragments arranged by size: 3518 2070 540 210 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6466 XbaI T'CTAG_A Cuts at: 3802 3802 Size: 6466 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6466 XmaI C'CCGG_G Cuts at: 1870 2097 3791 1870 Size: 227 1694 4545 Fragments arranged by size: 4545 1694 227 XmnI GAAnn'nnTTC Cuts at: 4595 4595 Size: 6466 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3181 4274 276 Size: 53 83 186 936 1647 1093 2468 Fragments arranged by size: 2468 1647 1093 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI XcmI